Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,507 G→T 8.5% S77* (TCA→TAA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5070GT 8.5% 988.7 / 9.2 341S77* (TCA→TAA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (161/136);  new base T (12/32);  total (173/168)
Fisher's exact test for biased strand distribution p-value = 1.07e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

CGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAA  >  NC_000913/238358‑238656
                                                                                                                                                      |                                                                                                                                                     
     gggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAtttt                                                                                                                                                  >  1:6216715/1‑150 (MQ=255)
       gTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTttat                                                                                                                                                <  1:7414064/150‑1 (MQ=255)
            aaTTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTTTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATct                                                                                                                                           >  1:8648389/1‑149 (MQ=255)
             aTTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgt                                                                                                                                          <  1:2204007/150‑1 (MQ=255)
              ttAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgta                                                                                                                                         <  1:6851780/150‑1 (MQ=255)
               tAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaa                                                                                                                                        <  2:8413834/150‑1 (MQ=255)
               tAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaa                                                                                                                                        <  1:5210325/150‑1 (MQ=255)
                     tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                                  <  2:886879/150‑1 (MQ=255)
                     tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                                  <  1:1404156/150‑1 (MQ=255)
                       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                >  2:3842771/1‑150 (MQ=255)
                       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                >  1:4035731/1‑150 (MQ=255)
                        cATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTa                                                                                                                               <  1:4597607/150‑1 (MQ=255)
                          tctCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                             <  1:6001739/148‑1 (MQ=255)
                          tctCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                             <  2:6339685/148‑1 (MQ=255)
                            tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                           >  1:5543959/1‑150 (MQ=255)
                            tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                           >  2:502632/1‑150 (MQ=255)
                               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        >  1:3653853/1‑150 (MQ=255)
                               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        <  2:4356238/150‑1 (MQ=255)
                                gtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCTTAAGTAAAATTACATGAata                                                                                                                       >  2:8553392/1‑150 (MQ=255)
                                     aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  >  1:3940984/1‑150 (MQ=255)
                                     aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  >  2:6586166/1‑150 (MQ=255)
                                       aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaa                                                                                                                <  2:3496153/150‑1 (MQ=255)
                                         ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAg                                                                                                              >  2:118800/1‑150 (MQ=255)
                                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                        >  1:8557504/1‑150 (MQ=255)
                                                 tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                      <  2:5367760/150‑1 (MQ=255)
                                                   aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                    >  2:6784178/1‑150 (MQ=255)
                                                   aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                    >  1:729173/1‑150 (MQ=255)
                                                      gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGa                                                                                                 >  1:2381583/1‑150 (MQ=255)
                                                        gCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaaa                                                                                               <  2:7199651/150‑1 (MQ=255)
                                                           aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                            <  2:4186032/150‑1 (MQ=255)
                                                             tACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGaa                                                                                          <  2:7101969/150‑1 (MQ=255)
                                                              actaTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                         <  2:7625205/147‑1 (MQ=255)
                                                              aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                         >  1:1730908/1‑150 (MQ=255)
                                                              aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                         >  1:6104813/1‑150 (MQ=255)
                                                                   aGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAg                                                                                    >  1:6450423/1‑150 (MQ=255)
                                                                     cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTTTGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                  <  2:2710101/150‑1 (MQ=255)
                                                                     cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                  <  2:7871781/150‑1 (MQ=255)
                                                                     cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                  >  2:5145167/1‑150 (MQ=255)
                                                                     attttattaAAAATATAATTGTTCGGTGTTTTTTTTTGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTGTTTAATATTTATTTTTTTTTCTTATTAAAAATTAAATTAATTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGaa                                                                                  <  1:1965291/147‑1 (MQ=255)
                                                                      tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAAt                                                                                 >  2:6969468/1‑150 (MQ=255)
                                                                         tAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCaa                                                                              <  2:6278728/150‑1 (MQ=255)
                                                                           gTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                            >  1:7797092/1‑150 (MQ=255)
                                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                            >  2:4543087/1‑150 (MQ=255)
                                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                           >  2:1760016/1‑150 (MQ=255)
                                                                              aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                         >  1:7878980/1‑150 (MQ=255)
                                                                              aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATtc                                                                         >  1:2889199/1‑150 (MQ=255)
                                                                                    taAGTGTTCGCTGTTTTGTTATGTCTGTGGCGAAATGGACTACCTTCTTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                   <  2:8157075/150‑1 (MQ=255)
                                                                                    taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                   <  2:1881080/150‑1 (MQ=255)
                                                                                     atgtgTTAGCTTTTTTGTTTTGTCGGTGGATAATTTGACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCTTAATAAAAATTACATTAATATTAAAAATTTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                                  <  1:3142258/146‑1 (MQ=255)
                                                                                     aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                                  >  2:2611032/1‑150 (MQ=255)
                                                                                               tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAAATAAATGAATATTTAAAAAGGGAAACCAAATGAAACCCAAGCACAGAATCAAAAAAAAACAATCAAAAAATAtt                                                        >  1:3500800/1‑150 (MQ=255)
                                                                                                gTATTTTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                       <  1:2043773/150‑1 (MQ=255)
                                                                                                gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                       >  2:5142705/1‑150 (MQ=255)
                                                                                                 ttttGTTATGTCTGTGGCTAAATTGACTACATCCGTTTTTTTGAAAAATAATGATTTTATTATCGAAATTAAAATTACATTAATATTTAAATTGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                      <  1:690674/148‑1 (MQ=255)
                                                                                                 tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACACAAAAAACAATCTACCATCATAAAATAATTc                                                      >  1:8128701/1‑150 (MQ=255)
                                                                                                  aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAAACGAAGCACAGAAAAAACAATATCCAAAAAAAAAAAAtttac                                                     >  1:294887/1‑148 (MQ=255)
                                                                                                   ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCg                                                    >  2:1526909/1‑150 (MQ=255)
                                                                                                     tttttGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTTTTTAATAATTATTTTATTATCTTAATTAAAATTAATTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                  <  1:1525931/146‑1 (MQ=255)
                                                                                                     gTTATGTCTGTGGCTAAATTGACTACATTCTTTTTTTTGATTAAGAATTATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                  <  1:1114398/150‑1 (MQ=255)
                                                                                                     gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATTATTTTATTTTCGTAAGTAAAATTAAATGAATAGTTAAAAAGGAAAACGAAATTAAAAAGAATCAAAGGAAAAAAAATATACAATAAAAAAATATTTACGtt                                                  >  1:7129131/1‑149 (MQ=255)
                                                                                                     gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATAGTAAGTAAAATTACATGAAAAATTAAAAAGGAAAAACACATGAAAACGAAGCACAGAATCAAAAATAACCAAAAATAAAAAATTTCCGTg                                                  >  1:2788420/1‑150 (MQ=255)
                                                                                                     gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                  <  2:8296127/150‑1 (MQ=255)
                                                                                                       tATTTCTGTGGCGAAATTTACTCCCTTCGTTTTTTTTATTAAGAATTTTTTTATTATCGTAATTAAATTTAAATTAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                <  2:3710414/150‑1 (MQ=255)
                                                                                                        aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                               >  2:5730955/1‑150 (MQ=255)
                                                                                                         tGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGc                                              >  2:590756/1‑150 (MQ=255)
                                                                                                         tGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGc                                              >  2:5005249/1‑150 (MQ=255)
                                                                                                             tgtgGCGAAATTGACTACCTTCGTGTTTTTGTTTTAGAATTTTTTTATTATCGTAATTAAAATTAAAAGAATATTTAAAAAGGGAAAAGACAAGAAAAACAAGCCCACAAAAAACAAACTACAAAAAAAAAAAATTTACGTGGTGCCttt                                          >  1:47654/1‑150 (MQ=255)
                                                                                                               tgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                        >  2:2943498/1‑150 (MQ=255)
                                                                                                                  cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAGTttatta                                     >  2:8037665/1‑150 (MQ=255)
                                                                                                                    aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  1:1092658/150‑1 (MQ=255)
                                                                                                                    aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  1:1869125/150‑1 (MQ=255)
                                                                                                                      aTTGACTACCTTCTTTTTTTTTATTAATAATTATTTTATTATCGTATTTAAAATTACTTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                 <  1:5251052/150‑1 (MQ=255)
                                                                                                                        tGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAat                               <  2:5556636/150‑1 (MQ=255)
                                                                                                                            tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                           >  2:8492582/1‑150 (MQ=255)
                                                                                                                             aCCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                          <  1:797167/150‑1 (MQ=255)
                                                                                                                             aCCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                          <  1:8317174/150‑1 (MQ=255)
                                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                         <  1:5669526/150‑1 (MQ=255)
                                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                         <  2:7933667/150‑1 (MQ=255)
                                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCAAAGAATCAACATTCTCCAATGATAAAATATTTAGGTTGAGGATTTTATTATTTAATATagag                         >  2:4478619/1‑150 (MQ=255)
                                                                                                                                ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATATAGGt                       >  2:7789486/1‑150 (MQ=255)
                                                                                                                                 tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAATCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGtt                      >  1:7884332/1‑150 (MQ=255)
                                                                                                                                     tttttttGATTAAGAATTATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                   <  1:4707909/150‑1 (MQ=255)
                                                                                                                                     tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                   <  2:2161697/150‑1 (MQ=255)
                                                                                                                                        ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                <  2:2039633/150‑1 (MQ=255)
                                                                                                                                         tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc               >  2:4056422/1‑150 (MQ=255)
                                                                                                                                          ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGTATTTTATTATTGAATATAGAGGTTTAACTCCg              >  1:2622356/1‑150 (MQ=255)
                                                                                                                                          ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg              <  2:1244618/150‑1 (MQ=255)
                                                                                                                                             aTTAAGAATTATTTTATTATCTTAAGTAAAATTACATTTATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa           <  1:502632/150‑1 (MQ=255)
                                                                                                                                             aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa           >  1:8204645/1‑150 (MQ=255)
                                                                                                                                              ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa          >  1:3059763/1‑150 (MQ=255)
                                                                                                                                              ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa          >  2:282311/1‑150 (MQ=255)
                                                                                                                                               taataaTGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa         <  2:3002303/150‑1 (MQ=255)
                                                                                                                                                aaGAATGATTTTATTATCGTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa        <  2:2380797/150‑1 (MQ=255)
                                                                                                                                                  gAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa      <  1:4130985/150‑1 (MQ=255)
                                                                                                                                                  gAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa      <  2:6103956/150‑1 (MQ=255)
                                                                                                                                                   aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc     <  2:730652/150‑1 (MQ=255)
                                                                                                                                                   aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc     >  2:3222212/1‑150 (MQ=255)
                                                                                                                                                    aTGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa    <  2:5309800/150‑1 (MQ=255)
                                                                                                                                                     tGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa   >  2:4708462/1‑150 (MQ=255)
                                                                                                                                                      gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa  >  1:4421755/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAA  >  NC_000913/238358‑238656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: