Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,427 C→A 22.8% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4270CA22.8% 783.9 / 81.6 346Q433K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (81/176);  new base A (83/5);  total (165/181)
Fisher's exact test for biased strand distribution p-value = 7.14e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 356 total aligned reads displayed.

TTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCC  >  NC_000913/2470278‑2470576
                                                                                                                                                     |                                                                                                                                                     
ttGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTc                                                                                                                                                       <  1:5844471/150‑1 (MQ=255)
 tGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCa                                                                                                                                                      <  2:7851815/150‑1 (MQ=255)
  gtagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTaaa                                                                                                                                                     >  2:2772233/1‑150 (MQ=255)
  gtagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaa                                                                                                                                                     <  2:6612246/150‑1 (MQ=255)
       aTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATaaa                                                                                                                                                >  1:6335403/1‑150 (MQ=255)
        tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAACAAAt                                                                                                                                               >  1:5512377/1‑150 (MQ=255)
          tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATTAAATAATTAAAAGAAAtac                                                                                                                                             >  1:6806823/1‑148 (MQ=255)
          tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAACa                                                                                                                                             >  1:3071438/1‑150 (MQ=255)
           ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                            <  2:3569016/150‑1 (MQ=255)
           ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                            <  2:1871873/150‑1 (MQ=255)
                  gAATATCTCATTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                     <  1:4621141/150‑1 (MQ=255)
                  gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGTAATAAAGACAATa                                                                                                                                     >  1:7836062/1‑150 (MQ=255)
                  gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACaaaa                                                                                                                                     >  2:6083824/1‑148 (MQ=255)
                  gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAATAAAGAAAATa                                                                                                                                     >  2:1709172/1‑150 (MQ=255)
                   aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                    <  1:5383238/150‑1 (MQ=255)
                   aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATCAAGACAATat                                                                                                                                    >  1:4900936/1‑149 (MQ=255)
                        ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCTTTTTTTTTATTTTTTTAATAATTAAAATTAATAAAGAAAAtttgtttt                                                                                                                               >  1:4928393/1‑143 (MQ=255)
                        ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAAAAGCGAtt                                                                                                                               >  2:7946305/1‑149 (MQ=255)
                        ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAAAAAAGAAAAAAACGAtt                                                                                                                               >  1:7144166/1‑149 (MQ=255)
                         tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGt                                                                                                                              >  1:3385812/1‑150 (MQ=255)
                           aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATTTaa                                                                                                                            >  2:7086723/1‑150 (MQ=255)
                           aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAAAAAGACAAAAGCGATGTaa                                                                                                                            >  1:4259865/1‑150 (MQ=255)
                            aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                           <  2:7034477/150‑1 (MQ=255)
                              tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                                                                                                         <  2:2278409/150‑1 (MQ=255)
                               ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                        <  2:55926/150‑1 (MQ=255)
                                 aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                                                                                                      <  1:2576519/150‑1 (MQ=255)
                                   gtgtCATTTTTCGGGGAATGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                    <  1:7006656/150‑1 (MQ=255)
                                   gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                    <  1:7237175/150‑1 (MQ=255)
                                   gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                    <  1:3989269/150‑1 (MQ=255)
                                   gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTAAAAAAAAAAAATAAAAAAGCGATGTAAAggcagaa                                                                                                                    >  1:6033477/1‑144 (MQ=255)
                                      tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                 <  2:4515183/150‑1 (MQ=255)
                                      tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAATACAAAAGCGATGTAAAGCACAAAGtt                                                                                                                 >  2:4391754/1‑150 (MQ=255)
                                      tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                 >  2:4694049/1‑150 (MQ=255)
                                         tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAAAAGCGATGTAAAGCACAAAGTTTaa                                                                                                              >  2:7262014/1‑150 (MQ=255)
                                          ttttCGGGGAAGGATATAGATATTTAAGCAATCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                             <  1:7368122/150‑1 (MQ=255)
                                          ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                             <  2:4889681/150‑1 (MQ=255)
                                               ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAATAAATAAAGACAAAAGCGATGTAAAGCACGAAGTTTAAAtttag                                                                                                        >  1:2947886/1‑147 (MQ=255)
                                                gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                       <  1:5139272/150‑1 (MQ=255)
                                                       atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATAAAATAACGATGTAAAGCACGAAGTTTAAATTTCGCGCCCATc                                                                                                >  1:8531665/1‑150 (MQ=255)
                                                       atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACATTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATc                                                                                                <  2:4957965/150‑1 (MQ=255)
                                                           agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                            >  2:2186699/1‑150 (MQ=255)
                                                           agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAATAAAGAAAAAAGAGATGTAAAGCACAAAGTTTAAATTTCGCGCCaaaaaatg                                                                                            >  2:7637893/1‑144 (MQ=255)
                                                           agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTTTAAAATAAATAAAAGAAAAAAACAAAAAAGCGGAGTAAAACAAAAAGTTTTAATTTAGCGCCCATCAAgg                                                                                            >  1:1972148/1‑150 (MQ=255)
                                                            gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAAAAAAAAAGAAAAAAGCGATGTAAAGCACAAAGTTTAAATTTCGCGCCAAGCagtgc                                                                                           >  1:7941121/1‑146 (MQ=255)
                                                             agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcg                                                                                          <  2:2528108/150‑1 (MQ=255)
                                                               aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcg                                                                                        <  2:5111574/150‑1 (MQ=255)
                                                               aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcg                                                                                        <  2:8370907/150‑1 (MQ=255)
                                                                 ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGca                                                                                      <  2:5245257/150‑1 (MQ=255)
                                                                 ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                      <  2:6391782/150‑1 (MQ=255)
                                                                  tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac                                                                                     <  2:5571846/150‑1 (MQ=255)
                                                                     gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAAGACAAAATCGATGTAAAAAACAAAGTTTTAATTTAGCGCCAAACATTGCGCGCCCaaa                                                                                  >  1:4748115/1‑149 (MQ=255)
                                                                      caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAAAAGCGATGTAAAGCAAAAAGTTTAAAATTCGCGCCAATAAATGCGCGCACaaac                                                                                 >  2:5304776/1‑148 (MQ=255)
                                                                        agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                               <  2:8236702/150‑1 (MQ=255)
                                                                        agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAAAAAAAAAAAAAAAAAAAAAAAGAGATTTAAATCACAAAGTTTTAAATTCGCGCCAATCATTTCGCGCACAAACta                                                                               >  2:8067674/1‑150 (MQ=255)
                                                                           atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                            <  2:442386/150‑1 (MQ=255)
                                                                                  tttattttATTTTTCGTTCTACATTTGATTATAAAAAACAGTATTTTTTTTAATTTATAAAATATTTAAAATAAATCAAGACAATAGCTATTTAAAGCACTAATTTTAATTTGCGCGCCAATCTGGGCGCGCACAAGCTATAATACCAAc                                                                     <  1:2131128/143‑1 (MQ=255)
                                                                                  tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTTAAAAAAATAAAAGAAAAAAAGACAAAAGAGAAGTAAAGCACAAAAAATAAATATGGCGCCCAACAAGGCGCGCACCCGCTAAAATACCAAc                                                                     >  1:8654978/1‑150 (MQ=255)
                                                                                      aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTaa                                                                 <  2:7524943/150‑1 (MQ=255)
                                                                                          ttttttCGTTCCACTTTTTCTTAAAAAAAACATTTTTTTTTTTAATTTATAAAATAATTCAAACAAATAAAGACAATAGCGATGTAAAGAAATAATTTTAAATTGCGCGCCAATCAGGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                             <  1:2361294/149‑1 (MQ=255)
                                                                                           tttttCGTTCGACCTTTGATTATACATAACAGTCGTTTTTTTAAGTTATAAAATAATTAAAATAAATAAAGACAATAGCGAAGTAAAGCACAAAGTTTAAATTGCGCCCCAATCAAGGCGCGCACAAGCTATAATACCAACCTAAATtcg                                                            >  2:6813405/1‑149 (MQ=255)
                                                                                            ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                           <  2:4905043/150‑1 (MQ=255)
                                                                                                 gagcgACCCTTGCTTATCCAAACCAGTCGTTGATTTAATTTATAAAATAATTCAAAGAAACCAAGACAATAGCGATGTAAAGCACTACGTTTAAATGGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                      <  2:6048524/147‑1 (MQ=255)
                                                                                                  ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAAAAGCGAAGTAAAGCACTAAGATTAAATTTCGCGCCAAACAAGGCGCGCACAAGCTATAATAACAAACAAATTTatccaccac                                                     >  2:1556681/1‑148 (MQ=255)
                                                                                                   tcgACCTTTTATTATACATAACAGTCTTTTTTTTATTTTATAAAATAATTAAAATAAATCAAGAAAATAGCGATGTAAAGCACTATTTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCctct                                                    <  1:3253313/150‑1 (MQ=255)
                                                                                                      aCCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGGTTAAAATTCGCGCCCATAAAGGCGCGCCCCAGCAATAATAACAACCAAAATTATCCTCCGCTTag                                                 >  2:1806313/1‑150 (MQ=255)
                                                                                                       ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                <  2:3365508/150‑1 (MQ=255)
                                                                                                        cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATCAAGACAAAATCGATTTAAAGCACTAAGTTTAAAATGCGCGCCCATCATGGCGCGCACAAGCAATAAAACCAACCAAATTTATACTCCGCTTagag                                               >  2:3125857/1‑150 (MQ=255)
                                                                                                           tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                            <  2:5660633/150‑1 (MQ=255)
                                                                                                              ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCGTTGTAAAGGAGTAATTTTAAATTGCGGGGCAATGATGGGGCGCGCAAGGTATTTTAGCAACCTAATTTGTGCTCCTATTTGAGTgtgtt                                         >  2:2130026/1‑146 (MQ=255)
                                                                                                                ataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAACAAAACAAGACCAAACCCATGTAAAGCACAAACTTTAAAATGCGCGCCAAAAAAGGCGCGCACAACCAAAAAAACCAACCAAAATTCTCCACCACATAGAGTGACaata                                       >  2:2612165/1‑150 (MQ=255)
                                                                                                                ataCATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAACAAGACAAAAGCGATGTAAAGCACGAAGTTTAAATTGCCCGCCAATCATGGCGCGCACAAGCTATAAAACCAACCAAATTTCTCCTCCACATAGAGTGACtata                                       >  2:6487039/1‑150 (MQ=255)
                                                                                                                  aCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAACCTATAATACCAACCTAAATTCTACTCCACTTAGAGTGACTatata                                     >  2:2057655/1‑149 (MQ=255)
                                                                                                                  aCATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAAAAACACAAAATCGATGTAAAACACTAAGTTTAAAATGAGCGCCCATCATGGCGCGCACCAGCTATAAAAACAAACAAAATTCTACTCCACATAGAGTGACTATAtc                                     >  2:2435350/1‑150 (MQ=255)
                                                                                                                   cATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATATCGATGTAAAGCACAAAGTTTAAATTTAGCGCCAATAAAGGCGCGCACAAGCTATAATACAAAACTAATTTATACTACTCTTAGATTTACTATAtct                                    >  2:1254875/1‑150 (MQ=255)
                                                                                                                    aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                   <  2:8393671/150‑1 (MQ=255)
                                                                                                                    aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                   <  2:6894840/150‑1 (MQ=255)
                                                                                                                     tAACATTCTTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGATATAATACCAACCTAATTTCTCCTCCTCTTATAGTGACTATATCTcc                                  <  2:2947886/150‑1 (MQ=255)
                                                                                                                     tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                  <  2:15893/150‑1 (MQ=255)
                                                                                                                      aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                 <  2:4486506/150‑1 (MQ=255)
                                                                                                                           tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGAACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                            >  2:4209185/1‑150 (MQ=255)
                                                                                                                             gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                          <  1:1557165/150‑1 (MQ=255)
                                                                                                                             gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                          >  1:1169972/1‑150 (MQ=255)
                                                                                                                                 ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                      <  1:3684238/150‑1 (MQ=255)
                                                                                                                                  tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                     <  1:6370488/150‑1 (MQ=255)
                                                                                                                                  tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                     <  2:8565928/150‑1 (MQ=255)
                                                                                                                                  tatAATTTATAAAAAAATACAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTACAGTGACTATATCTCCTGATAGAATTGCg                     <  2:8141114/148‑1 (MQ=255)
                                                                                                                                   ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                    >  1:2869948/1‑150 (MQ=255)
                                                                                                                                       tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAATCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                <  2:7714651/150‑1 (MQ=255)
                                                                                                                                       tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTCGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                <  2:4070347/150‑1 (MQ=255)
                                                                                                                                       tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                <  1:8317757/150‑1 (MQ=255)
                                                                                                                                        ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGGTAGAATTGCGGTATTg               >  1:6092408/1‑150 (MQ=255)
                                                                                                                                         tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa              <  1:2645700/150‑1 (MQ=255)
                                                                                                                                           taAGATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGAACTAAGTTTAAATTGCGCGCCAAGCAGGGCGCGAAAAAGCAATAAAAAAAACCAAATATCTAATAAACTAAGAGTGACTAAATCACCTGAAAGAATAGCGGTaaatact            >  1:1794047/1‑144 (MQ=255)
                                                                                                                                            aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGTATTGCGGTATTGACTa           <  2:3474222/150‑1 (MQ=255)
                                                                                                                                             aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt          >  2:1468803/1‑150 (MQ=255)
                                                                                                                                              aataatTCAAAGAAATCAAGACAATAGCGTTGTAAAGCTCTATGTTTAATTTGCGGGGCTATCATGGCGCGCACTAGCTATAATACCAACCTAATTTCTCGTGCTCTTTGAGTGACTATATCTGGTGATAGAATTGCGGTTTTgtgtatg         >  2:5451360/1‑144 (MQ=255)
                                                                                                                                                  atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaan     <  1:4459785/150‑2 (MQ=255)
                                                                                                                                                   ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg    >  2:4831153/1‑150 (MQ=255)
                                                                                                                                                    tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc   >  1:5275601/1‑150 (MQ=255)
                                                                                                                                                     cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc  >  1:4711936/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCC  >  NC_000913/2470278‑2470576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: