Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,481,750 C→A 5.9% A143S (GCT→TCT)  emrY ← putative multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,481,7500CA 5.9% 1801.3 / 19.3 525A143S (GCT→TCT) emrYputative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (258/220);  new base A (2/45);  total (260/265)
Fisher's exact test for biased strand distribution p-value = 2.66e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 534 total aligned reads displayed.

TAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAA  >  NC_000913/2481601‑2481899
                                                                                                                                                     |                                                                                                                                                     
taagGTTAAGCAAAATGTCAGGACGATAATCCCCATAGGGACAATGAATAAAAATATCCAACCCCACCTAAACTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGc                                                                                                                                                       <  2:369669/150‑1 (MQ=255)
  agGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCaa                                                                                                                                                     <  2:4857968/150‑1 (MQ=255)
       aaGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGtt                                                                                                                                                <  2:5681818/150‑1 (MQ=255)
          cATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCtt                                                                                                                                             <  1:6343415/150‑1 (MQ=255)
          cATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCtt                                                                                                                                             >  2:4995166/1‑150 (MQ=255)
          cATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCtt                                                                                                                                             >  2:2321077/1‑150 (MQ=255)
          cATAAAGTCAGGACGATAAACCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCtt                                                                                                                                             >  1:2851417/1‑150 (MQ=255)
           aTAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCttt                                                                                                                                            >  1:8463278/1‑150 (MQ=255)
             aaTGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCttttt                                                                                                                                          >  2:3882048/1‑150 (MQ=255)
              aTGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCtttttt                                                                                                                                         >  1:5774056/1‑150 (MQ=255)
              aTGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCtttttt                                                                                                                                         <  2:6219200/150‑1 (MQ=255)
                  cAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTgg                                                                                                                                     <  2:6939833/150‑1 (MQ=255)
                   aGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGc                                                                                                                                    >  2:2561514/1‑150 (MQ=255)
                    ggACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCtttttt                                                                                                                                         <  1:4191019/144‑1 (MQ=255)
                    ggACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCg                                                                                                                                   >  1:5207871/1‑150 (MQ=255)
                      aCGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGa                                                                                                                                 <  2:6623117/150‑1 (MQ=255)
                      aCGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGa                                                                                                                                 <  1:976272/150‑1 (MQ=255)
                        gATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATa                                                                                                                               >  1:7987175/1‑150 (MQ=255)
                          taaaacccATAGGGACATTGATTAAAAATATCAAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAAAAACGGCCCACATATCGGAGCGATAAACACGGTCATTGACCATAATGCCAGAGAAAATGTTCTTTTTTCTGGCGTATAAt                                                                                                                             >  1:4994479/6‑150 (MQ=255)
                           aaTCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAAtt                                                                                                                            <  1:3453033/150‑1 (MQ=255)
                           aaTCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAAtt                                                                                                                            <  1:1019389/150‑1 (MQ=255)
                              ccccATAGGGACATTGATTAAATATATCCAACCCCCGCTAACGTTATCACAAATATAACCGCCCAAGATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATTATTCCt                                                                                                                         <  2:5615274/150‑1 (MQ=255)
                              ccccATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCt                                                                                                                         <  1:1515282/150‑1 (MQ=255)
                                 cATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaa                                                                                                                      <  1:7854314/150‑1 (MQ=255)
                                 cATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaa                                                                                                                      >  1:2515087/1‑150 (MQ=255)
                                  aTAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaat                                                                                                                     <  2:8110823/150‑1 (MQ=255)
                                  aTAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaat                                                                                                                     <  1:7706528/150‑1 (MQ=255)
                                   tAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaata                                                                                                                    <  2:2003111/150‑1 (MQ=255)
                                   tAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaata                                                                                                                    <  1:5571293/150‑1 (MQ=255)
                                    aGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaataa                                                                                                                   >  2:4449295/1‑150 (MQ=255)
                                      ggACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCACTATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaataata                                                                                                                 <  2:6329220/150‑1 (MQ=255)
                                        aCATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATaaa                                                                                                               >  2:3529565/1‑150 (MQ=255)
                                        aCATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATaaa                                                                                                               <  1:4035815/150‑1 (MQ=255)
                                                tAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGa                                                                                                       >  1:5741603/1‑150 (MQ=255)
                                                  aaaaTATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACa                                                                                                     >  1:5683153/1‑150 (MQ=255)
                                                  aaaaTATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACa                                                                                                     >  1:5005276/1‑150 (MQ=255)
                                                   aaaTATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAg                                                                                                    >  2:8589934/1‑150 (MQ=255)
                                                        tCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGaa                                                                                               >  1:7275905/1‑150 (MQ=255)
                                                          cAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAAtt                                                                                             <  1:1796871/150‑1 (MQ=255)
                                                          cAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTCTGACAGTGGAAta                                                                                             >  1:5307378/1‑149 (MQ=255)
                                                               ccAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACgg                                                                                        >  1:3112033/1‑150 (MQ=255)
                                                               ccAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGACCACATATCGGAGCGATAATCACGGTCATTGACCCGAATGCCAGCGCACATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACgg                                                                                        >  2:4242789/1‑150 (MQ=255)
                                                                  gCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGccc                                                                                     >  2:1683297/1‑150 (MQ=255)
                                                                  gCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGccc                                                                                     <  2:601971/150‑1 (MQ=255)
                                                                    tAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCg                                                                                   >  2:5204703/1‑150 (MQ=255)
                                                                     aaaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGc                                                                                  >  1:2357900/1‑150 (MQ=255)
                                                                        gTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCAt                                                                               >  1:4338586/1‑150 (MQ=255)
                                                                         ttATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCAtt                                                                              >  1:586642/1‑150 (MQ=255)
                                                                           aTCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTaa                                                                            >  2:8274137/1‑150 (MQ=255)
                                                                             cacaAATATAACCGCCCAATACCGGCCCACATATCGGAGCTATAATCACGTTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGTAATTAACGGCCCCGCCATTAAcc                                                                          <  1:3870000/150‑1 (MQ=255)
                                                                              acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                         >  1:6891304/1‑150 (MQ=255)
                                                                              acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                         <  2:7037342/150‑1 (MQ=255)
                                                                              acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                         >  2:4066730/1‑150 (MQ=255)
                                                                               caAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGTAATTAACGGCCCCGCCATTAAcccc                                                                        <  1:661418/150‑1 (MQ=255)
                                                                               caAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAcccc                                                                        <  2:2449473/150‑1 (MQ=255)
                                                                                aaaTATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCt                                                                       >  2:7799648/1‑150 (MQ=255)
                                                                                aaaTATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCt                                                                       >  2:848687/1‑150 (MQ=255)
                                                                                 aaTATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTg                                                                      <  2:1919236/150‑1 (MQ=255)
                                                                                   tataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGaa                                                                    >  1:4852350/1‑150 (MQ=255)
                                                                                   tataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGACATTGACCATAATGCCAGAGAAAATTTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGaa                                                                    <  1:3671811/150‑1 (MQ=255)
                                                                                      aaCCGCCCAATATCGGCCAACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTATTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGa                                                                 <  1:2131006/150‑1 (MQ=255)
                                                                                        ccGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGAct                                                               >  1:2028610/1‑150 (MQ=255)
                                                                                        ccGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGAct                                                               >  1:1368420/1‑150 (MQ=255)
                                                                                         cGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActc                                                              >  1:2924274/1‑150 (MQ=255)
                                                                                         cGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActc                                                              >  1:7395792/1‑150 (MQ=255)
                                                                                         cGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActc                                                              >  2:7380068/1‑150 (MQ=255)
                                                                                         cGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGCGGAATTAACGGCCCCGCCATTAACCCCTGAACGActc                                                              >  1:5981536/1‑150 (MQ=255)
                                                                                           cccAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTa                                                            >  2:1844338/1‑150 (MQ=255)
                                                                                               atctCGGCCCACATATCGGTGCGATATTCCCGGTCATTGCCCATATTCCCCGAGCAAATGTTCTTTTTTTTGGGGGATAATCCCTTAATATTAAACTCTGTTACAGTGGTTTTAACGGCCCGGCATTTAACCCCTGAACGACTCTaaaaa                                                        <  2:2997235/147‑1 (MQ=255)
                                                                                               atatCGGCCCACATATCGTAGCGATATTCACGGTCATTTACCATAATGCCAGAGAAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaaaa                                                        <  2:4921483/150‑1 (MQ=255)
                                                                                               atatCGGCCCACATATCGGAGCGATAATCAGGGTCATTGACCATAATGCCAGAGAAATTTTTCTTTTTTCTGGGGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaaaa                                                        <  2:1316859/150‑1 (MQ=255)
                                                                                               atatCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTcaaaa                                                        >  2:4359201/1‑150 (MQ=255)
                                                                                                tatCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATTTTCTTTTTTCTGGCGGATAATTCATTATTAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCTTTAACCCCTGAACGACTCTaaaaaa                                                       <  2:3577471/150‑1 (MQ=255)
                                                                                                     gCCCACATATCGTAGCGATAATCACGGTCTTTGACCATAAGGCCAGAGCAAATGTTCTTTTTTCTGGGGTTTAATTCCTTATTATTAAACTCTGTGACTGTGGAATTAACGGCCCCGCATTTAACCCCTGAACGACTCTAAAAAATATCa                                                  <  2:7544457/150‑1 (MQ=255)
                                                                                                     gCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTGAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCAGAACGACTCTAAAAAATATCa                                                  >  1:7126288/1‑150 (MQ=255)
                                                                                                      cccACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAg                                                 >  2:8662873/1‑150 (MQ=255)
                                                                                                        cacaTATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGca                                               >  1:4566811/1‑150 (MQ=255)
                                                                                                          caTATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGcaca                                             >  2:1144700/1‑150 (MQ=255)
                                                                                                                ggAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGa                                       >  1:8264251/1‑150 (MQ=255)
                                                                                                                  tgCGTTAATAACGGTAATTGACCATAATGCCAGATAAAATTTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTTACTGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAtt                                     <  2:7226228/149‑1 (MQ=255)
                                                                                                                  tgCGATAATCACGGTCATTGACCATAATGCCAGAGAAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGTAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAtt                                     <  2:4583351/149‑1 (MQ=255)
                                                                                                                   gCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTg                                    >  2:574504/1‑150 (MQ=255)
                                                                                                                     gATAATCACGGTCATTTACCATAATGCAATATAAATTGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTTTGACTGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                  <  2:4722232/150‑1 (MQ=255)
                                                                                                                          tCACGTTCTTTTACCATAATGCCTGAGCAATTGTTCTTTTTTCTGGGGTATATTTCCTTAATAATAAACTCTTTGACTGTGTATTTAAGGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATa                             <  1:4764579/150‑1 (MQ=255)
                                                                                                                          tCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATa                             >  2:2823740/1‑150 (MQ=255)
                                                                                                                            aCGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAgg                           >  2:3253124/1‑150 (MQ=255)
                                                                                                                            aCGGGCATTGACCATAATGCCAGAGCAAATGTTCATTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATAAAACCCTGAACGACTCTAAAAAATATCATCACAACAAGAGTGGTCGATAgc                           >  2:5436783/1‑149 (MQ=255)
                                                                                                                             cGGTAATTTACCAAAATGCCTGATAATATGTTTTTTTTTCTGGCTTATAATTCCTTAATATTAAACTCTTTGACTGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGc                          <  1:6729669/150‑1 (MQ=255)
                                                                                                                              ggTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCt                         >  1:6241355/1‑150 (MQ=255)
                                                                                                                                   ttGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTGCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAATAAATATCAGCACATCAAGATTGGTCGATAGGCTAcaca                    >  1:5478942/1‑150 (MQ=255)
                                                                                                                                    tGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAt                   >  1:2168025/1‑150 (MQ=255)
                                                                                                                                      aCCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTa                 >  1:1126552/1‑150 (MQ=255)
                                                                                                                                           aaTGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa            >  1:8625780/1‑150 (MQ=255)
                                                                                                                                                catagCAAATGTTCTTTTTTCTGGCGGATATTTCATTAATAATAAACTCTGTTACTGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAg       <  1:6496394/147‑1 (MQ=255)
                                                                                                                                                 agagCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGt                                                                                                   <  1:4644728/57‑1 (MQ=255)
                                                                                                                                                 agagCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGc      >  2:8380756/1‑150 (MQ=255)
                                                                                                                                                 agagAAAATGTTCTTTTTTGGGGCGGATATTTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGc      <  1:1669140/150‑1 (MQ=255)
                                                                                                                                                    gCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaa   >  2:1530529/1‑150 (MQ=255)
                                                                                                                                                     cAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaa  >  2:8022407/1‑150 (MQ=255)
                                                                                                                                                     cAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaa  >  1:1263578/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAA  >  NC_000913/2481601‑2481899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: