Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,523 G→T 9.6% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5230GT 9.6% 967.5 / 16.6 352L72I (CTT→ATT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (180/122);  new base T (5/43);  total (186/166)
Fisher's exact test for biased strand distribution p-value = 5.00e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 372 total aligned reads displayed.

AGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238374‑238672
                                                                                                                                                      |                                                                                                                                                     
 gTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagt                                                                                                                                                      <  1:3540271/150‑1 (MQ=255)
     tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                                                  <  1:4019688/150‑1 (MQ=255)
       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAtt                                                                                                                                                >  2:3842770/1‑150 (MQ=255)
         atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTaa                                                                                                                                              >  1:8157075/1‑149 (MQ=255)
          tctCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             <  2:6339685/148‑1 (MQ=255)
          tagccTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGAGAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             <  1:6001582/147‑1 (MQ=255)
            tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                                           >  2:502632/1‑150 (MQ=255)
              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                         >  2:1665062/1‑150 (MQ=255)
              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                         <  1:3368668/150‑1 (MQ=255)
              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAattaaattaa                                                                                                                                         >  2:8192741/1‑150 (MQ=255)
               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        <  2:4356238/150‑1 (MQ=255)
               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        >  2:1452770/1‑150 (MQ=255)
                  gACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGACTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTATTATGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAtt                                                                                                                                     <  1:2732970/150‑1 (MQ=255)
                     aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                  <  1:7521621/150‑1 (MQ=255)
                         ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                              >  1:6148877/1‑150 (MQ=255)
                         ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                              >  2:119716/1‑150 (MQ=255)
                         ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                              >  2:8468433/1‑150 (MQ=255)
                              tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                         <  1:1499810/150‑1 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                        >  1:8557504/1‑150 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                        >  1:245906/1‑150 (MQ=255)
                                 tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                                      >  1:2181668/1‑150 (MQ=255)
                                  aaaGGTGCTATTACGATAGCTATTATTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                     <  2:8344138/150‑1 (MQ=255)
                                  aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                     <  2:7151521/150‑1 (MQ=255)
                                   aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                                    >  2:6784178/1‑150 (MQ=255)
                                      gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGa                                                                                                                 >  1:2381583/1‑150 (MQ=255)
                                       tGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaa                                                                                                                >  2:1125323/1‑150 (MQ=255)
                                          tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                             >  2:8577355/1‑150 (MQ=255)
                                           aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                                            <  2:4186032/150‑1 (MQ=255)
                                           aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                                            >  2:6424260/1‑150 (MQ=255)
                                              aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                                         >  1:6104813/1‑150 (MQ=255)
                                                    gCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGa                                                                                                   >  2:5523377/1‑150 (MQ=255)
                                                     cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                  <  2:7871781/150‑1 (MQ=255)
                                                     attttattaAAAATATAATTGTTCGGTGTTTTTTTTTGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTGTTTAATATTTATTTTTTTTTCTTATTAAAAATTAAATTAATTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGaa                                                                                                  <  1:1965291/147‑1 (MQ=255)
                                                      tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                 >  2:2623608/1‑150 (MQ=255)
                                                       aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATAGTAAGTAAAATAAAATTAATATTTAAAAAGGAAAAAGACATGAAAAACAAGCACAGAAta                                                                                                >  1:3005910/1‑149 (MQ=255)
                                                       aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                >  2:689842/1‑150 (MQ=255)
                                                         tAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCaa                                                                                              <  2:6278728/150‑1 (MQ=255)
                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                                            >  2:4543087/1‑150 (MQ=255)
                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                           >  2:1760017/1‑150 (MQ=255)
                                                              aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCCAAGCACAGAATCAACATtc                                                                                         >  1:7878543/1‑150 (MQ=255)
                                                              aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATtc                                                                                         >  1:2889199/1‑150 (MQ=255)
                                                                aaTATAAGATTTTGCTTTTTTTTTTTGTCTGTGGCTAATTTGACTACCTTGTTTTTTTTTATTAATAATTATTTTTTTATCGTAATTAAAATTACATTAAAATTAAAAATTGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                       <  1:3827429/150‑1 (MQ=255)
                                                                   tttatttttAGCTTTTTTTTTTTGTCTGGGGTTAATTTGACTACATTCTTTTTTTTTATTAATATTTATTTTATTATCTTAATTAAAATTAAATTAATATTAAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                    <  1:1550930/143‑1 (MQ=255)
                                                                   atatgttttAGCTTTTTTTTTTTGTCTGTGGCTAATTTGACTCCCTTCTTTTTTTTTATTAATATTTTTTTTATTATCTTATTTAAAATTACATTAATATTTAAAAATTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                    <  1:7981794/143‑1 (MQ=255)
                                                                   ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                    >  2:478431/1‑150 (MQ=255)
                                                                    taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                   >  2:2789646/1‑150 (MQ=255)
                                                                               tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTTATAATTATTTTTTTATAGTAAGTAAAATTAAAAGAAAATTTTAAAAGGAAAAAGACATTAAAACGAATCACAGAATAAAAAATATACAATCaaaaaaaaat                                                                        >  1:5550257/1‑146 (MQ=255)
                                                                                gTATTTTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                       <  1:2043773/150‑1 (MQ=255)
                                                                                gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                       >  2:5142705/1‑150 (MQ=255)
                                                                                 ttttGTTATGTCTGTGGCGAATTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTATGTAAAATTAAATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                      <  1:689423/148‑1 (MQ=255)
                                                                                 tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAATAATGATTTTATTATAGTAAGTAAAAATAAATGAATATTTAAAAAGGAAAAAGACAAGAAAACGAAGCACAGAAAAAACATTCAACAAACATAAAATATTTc                                                                      >  1:6908108/1‑150 (MQ=255)
                                                                                 tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAACCGAATCACAGAATCAACATTCAACAATCAAAAAATAttta                                                                      >  1:7225179/1‑149 (MQ=255)
                                                                                 tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACAAGAAACCGAAGCACAGAAACAACATTCTCCAAAAATAAAATATTTc                                                                      >  2:3696118/1‑150 (MQ=255)
                                                                                  aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAAACGAAGCACAGAAAAAACAATATCCAAAAAAAAAAAAtttac                                                                     >  1:294887/1‑148 (MQ=255)
                                                                                         tGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGc                                                              >  2:5005249/1‑150 (MQ=255)
                                                                                             tgtgGCGAAATTGACTACCTTCGTGTTTTTGTTTTAGAATTTTTTTATTATCGTAATTAAAATTAAAAGAATATTTAAAAAGGGAAAAGACAAGAAAAACAAGCCCACAAAAAACAAACTACAAAAAAAAAAAATTTACGTGGTGCCttt                                                          >  1:47654/1‑150 (MQ=255)
                                                                                               tgGCTAAATCGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                        <  2:2344259/150‑1 (MQ=255)
                                                                                               tgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                        >  2:2943498/1‑150 (MQ=255)
                                                                                                 gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                      >  2:2843902/1‑150 (MQ=255)
                                                                                                  cGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTTTTATAGGAAGTAAAATTACATGAATATTTAAAAAGGGAAAACACAAGAAACCGCAGCACAGAAAAAACAATATCCAAACAAAAAAAATTTACGTGGAGCATTttatta                                                     >  1:1254988/1‑150 (MQ=255)
                                                                                                  cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAGTttatta                                                     >  2:8037665/1‑150 (MQ=255)
                                                                                                  cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                                     >  2:5788499/1‑150 (MQ=255)
                                                                                                   gAACTTGACTACCTTCGTTTTTTTGATCAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                    <  2:5942321/150‑1 (MQ=255)
                                                                                                    aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  1:1869125/150‑1 (MQ=255)
                                                                                                      aTTGACTACCTTCTTTTTTTTTATTAATAATTATTTTATTATCGTATTTAAAATTACTTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                                 <  1:5251052/150‑1 (MQ=255)
                                                                                                       tttaCTACCTTCTTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                <  2:5163608/147‑1 (MQ=255)
                                                                                                          aCTACCTGCGTTTTTTTGATTAAGAATGATTTTCTTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAatat                                             <  1:4571459/150‑1 (MQ=255)
                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  2:7933667/150‑1 (MQ=255)
                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCAAAGAATCAACATTCTCCAATGATAAAATATTTAGGTTGAGGATTTTATTATTTAATATagag                                         >  2:4478619/1‑150 (MQ=255)
                                                                                                                ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATATAGGt                                       >  2:7789486/1‑150 (MQ=255)
                                                                                                                     tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                   <  2:2161697/150‑1 (MQ=255)
                                                                                                                        ttttCATTAACAATTATATAATTAACTAAATTAAAATAAAATCAAAATTAAAAAACTAAAACGACATGAAACCGAACCACAGAAACAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                <  2:2500182/150‑1 (MQ=255)
                                                                                                                         tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               >  2:4056291/1‑150 (MQ=255)
                                                                                                                             aTTAAGAATTATTTTATTATCTTAAGTAAAATTACATTTATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                           <  1:502632/150‑1 (MQ=255)
                                                                                                                             aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                           >  1:8204645/1‑150 (MQ=255)
                                                                                                                             aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                           >  2:3421264/1‑150 (MQ=255)
                                                                                                                               tAAGAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                         <  2:3002550/150‑1 (MQ=255)
                                                                                                                                aagaaagaATTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCAGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                        >  1:3127970/1‑150 (MQ=255)
                                                                                                                                  gAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                      <  1:4130985/150‑1 (MQ=255)
                                                                                                                                  gAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                      <  2:6103956/150‑1 (MQ=255)
                                                                                                                                  gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                      <  1:5753879/150‑1 (MQ=255)
                                                                                                                                   aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTGAAAAAc                     >  1:7881038/1‑150 (MQ=255)
                                                                                                                                   aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                     <  2:730652/150‑1 (MQ=255)
                                                                                                                                    aTGATTTTTTTATCGTAAGTAAAATTACATGAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                    <  2:5309803/150‑1 (MQ=255)
                                                                                                                                      taTTTTATTATCTTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  <  1:832920/149‑1 (MQ=255)
                                                                                                                                      gATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  <  2:5684674/150‑1 (MQ=255)
                                                                                                                                      gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  >  1:4421755/1‑150 (MQ=255)
                                                                                                                                      gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  >  1:7918069/1‑150 (MQ=255)
                                                                                                                                      gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  >  2:2015434/1‑150 (MQ=255)
                                                                                                                                       aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                 >  1:103209/1‑150 (MQ=255)
                                                                                                                                         tttATTATCTTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa               <  2:7488963/150‑1 (MQ=255)
                                                                                                                                          ttattaTCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaag              <  2:32006/150‑1 (MQ=255)
                                                                                                                                            attatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGACTATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa            >  2:7021766/1‑150 (MQ=255)
                                                                                                                                            attatCGAAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa            <  1:5136225/150‑1 (MQ=255)
                                                                                                                                              tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          >  1:1105611/1‑150 (MQ=255)
                                                                                                                                              tatCGTAAGTAAAACTACAAGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATCAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          <  1:5527909/150‑1 (MQ=255)
                                                                                                                                                 cGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa       <  2:5349609/150‑1 (MQ=255)
                                                                                                                                                 cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa       <  1:6391790/150‑1 (MQ=255)
                                                                                                                                                   taagtaaAATTACATTAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg     <  1:1665211/150‑1 (MQ=255)
                                                                                                                                                     agtaaATTTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa   <  2:896240/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238374‑238672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: