Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,531,873 T→A 5.6% L189F (TTA→TTT)  yhgE ← DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,531,8730TA 5.6% 1511.5 / 7.9 476L189F (TTA→TTT) yhgEDUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (141/277);  new base A (39/2);  total (197/279)
Fisher's exact test for biased strand distribution p-value = 3.20e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 479 total aligned reads displayed.

TAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATG  >  NC_000913/3531725‑3532022
                                                                                                                                                    |                                                                                                                                                     
  aaCGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCCGCGCGGTAACCAGACCTAAc                                                                                                                                                    >  2:4512678/1‑150 (MQ=255)
    cGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGAATGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGt                                                                                                                                                  >  2:737843/1‑150 (MQ=255)
     ggCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACGGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCAAAAGTg                                                                                                                                                 >  2:8430618/1‑150 (MQ=255)
      gCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTga                                                                                                                                                <  2:5626429/150‑1 (MQ=255)
      gCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTga                                                                                                                                                <  2:7061225/150‑1 (MQ=255)
      gCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTga                                                                                                                                                <  2:1995141/150‑1 (MQ=255)
      gCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTga                                                                                                                                                <  1:2518843/150‑1 (MQ=255)
        aGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTgaga                                                                                                                                              <  2:7888359/150‑1 (MQ=255)
          ggTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAACCAGAACTAACGTGAGata                                                                                                                                            >  2:6398118/1‑150 (MQ=255)
               tgatAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACTTTACTGTTTTCGCGCGAGGATCAACCCCAGCGCGGTAACCACACCGAACGTGATaaaaaaaa                                                                                                                                       >  1:6069883/1‑150 (MQ=255)
                 atAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACCGGCCGGTGTTCGCGCCCGGCTCACCCCCCGCCCGGGAACCAGACCGCACGCGAGATACATAAAc                                                                                                                                     >  2:33707/1‑150 (MQ=255)
                  tAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCCGCGCGGTAACCAGACCTAACGTGAGATAAATAAAcc                                                                                                                                    >  1:5502140/1‑150 (MQ=255)
                      cATCTGGGTTAGCAATGATAATAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAGGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGTTAACCAGACCTAACGTGAGATAAATAAACCAGTc                                                                                                                                <  1:8156676/150‑1 (MQ=255)
                       aTCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGtag                                                                                                                               >  2:1906038/1‑148 (MQ=255)
                        tctgggTTAGCAATTATAATAACGGCAGCAACCCCGTGGCGATTAACTTGAACAACTTTTTAATAGAGTCTATTAAAGGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGGGGTAACCAGACATAACGTGAGATAAATAAACCAGTCgg                                                                                                                              <  1:1025204/147‑1 (MQ=255)
                        tCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAACCAGACCAAACGTGAGATAAATAAAACAGTCgg                                                                                                                              >  2:5363390/1‑150 (MQ=255)
                          aGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGtt                                                                                                                            <  2:5933939/150‑1 (MQ=255)
                                 gCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACGGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCaaaaa                                                                                                                     >  1:4345336/1‑150 (MQ=255)
                                    aTGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAaga                                                                                                                  <  2:1669742/150‑1 (MQ=255)
                                     tGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAagag                                                                                                                 <  1:1711828/150‑1 (MQ=255)
                                      gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGc                                                                                                                <  2:3543353/150‑1 (MQ=255)
                                        tACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGGTGCAAAAAAGAGCGt                                                                                                              >  1:3967392/1‑150 (MQ=255)
                                          cTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTAt                                                                                                            <  2:5988830/150‑1 (MQ=255)
                                          cTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACGGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTAt                                                                                                            >  1:1439089/1‑150 (MQ=255)
                                           tAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAAAGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGGAtt                                                                                                           >  2:4870872/1‑150 (MQ=255)
                                             aCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGGGACTGTGTTCGCGCGAGGATCACCGCCCGCGCGGGAACCAGACCGAACGGGAGATAAATAAACCCGTCGGGTGCAACAAAGAGCGGATTGa                                                                                                         >  1:7560374/1‑150 (MQ=255)
                                             aCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAAGAGAGTCGATTAAACGTGAATTTTTTAACGCGATGAACAACCCCAGCGCGGTAACCAGACCAAAAGTGAGATAAATAAACCAGTCCGTTGCAAAAAAGAGCGTaatta                                                                                                         >  1:613934/1‑146 (MQ=255)
                                                 cagcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCCCCAGCGCGGTAACCAGACCAAACGTGAGATAAAAAAAACAGTCGGTTGCAAAAAAGAGCGTATTTaaaaa                                                                                                     >  2:3024820/1‑150 (MQ=255)
                                                  agcaACCCCGTGGCTATTAACGTGAAAAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGTTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAAc                                                                                                    <  1:8068668/150‑1 (MQ=255)
                                                  agcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAAc                                                                                                    <  1:563358/150‑1 (MQ=255)
                                                  agcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGGAACCAGACCTAACGTGAGATAAATAAACCAGTCGGGTGCAAAAAAGAGCGTATTGAAAAAc                                                                                                    >  2:5762848/1‑150 (MQ=255)
                                                  agcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACGGTGTTAGCGCGAGGAAAACCGCCAGCGCGGTAACCAGACCGAACGGGAGATAAATAAACCAGTCGGGTGCAAAAAAGAGCGGATTTTAAAAc                                                                                                    >  2:5190060/1‑150 (MQ=255)
                                                    caACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGt                                                                                                  <  1:6463752/150‑1 (MQ=255)
                                                      aCCCCGTGGCGATTAACGTGAACATCTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATTAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGa                                                                                                <  2:4991777/150‑1 (MQ=255)
                                                      aCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGa                                                                                                <  2:4714290/150‑1 (MQ=255)
                                                      aCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGa                                                                                                <  2:8031826/150‑1 (MQ=255)
                                                       ccccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGAt                                                                                               >  2:1116609/1‑150 (MQ=255)
                                                        cccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGCTTGCAAAAAAGAGCGTATTGAAAAACGTGATg                                                                                              <  2:6645390/150‑1 (MQ=255)
                                                        cccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATCACAGCCAGCGCGGTAAACAGAACTAACGTGAGATAAATAAACCAGTAGGTTGCAAAAAAGTGCGTAATTAAAAAAGTGATg                                                                                              >  1:4649769/1‑150 (MQ=255)
                                                           gTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTTACTGTTTTCGCGCGTGGATCACCGCCAGCGCGGTAACCAGAACAAACGTGTGTTAAAAAAAACAGTCGGTTGCAAAAAATATCGTATTGAAAAACGTGTTGAca                                                                                           >  1:2952024/1‑150 (MQ=255)
                                                            tGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCcaa                                                                                          <  1:1193720/150‑1 (MQ=255)
                                                            tGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCcaa                                                                                          <  1:241229/150‑1 (MQ=255)
                                                            tGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATAACCGCCACCGCCGTAACCACACCAAACGTGAGATAAATAAACCAGTCGGTTGGAAAAAAGAGCGTATTGAAAAAACTTATGCcaa                                                                                          >  2:1496144/1‑150 (MQ=255)
                                                                gATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTAGCGCGAGGATAACCCCCAGCGCGGTAACCAGACCGAACGTGAGATAAATAAAACAGTCGGTTGCAAAAAAGAGCGTATTGTAAAACGTGATGCCAAAAAg                                                                                      >  2:3861865/1‑150 (MQ=255)
                                                                  ttAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGtt                                                                                    <  2:5536483/150‑1 (MQ=255)
                                                                    aaCGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTa                                                                                  <  1:6416553/150‑1 (MQ=255)
                                                                    aaCGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTa                                                                                  <  1:4489097/150‑1 (MQ=255)
                                                                     aCGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGATGATCACCGCCAGCGCGGTAACCAGACCAAAAGTGAGATAAAAAAAACACGCGGTTGCAAAAAATAGCGTATTGTAAAAAGTGTTTACCACAAGTTTaa                                                                                 >  1:7199888/1‑150 (MQ=255)
                                                                      cGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTaaa                                                                                <  2:4621919/150‑1 (MQ=255)
                                                                       gTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACAGTGTTAGCGCGAGGATCACCCCCAGCGCGGTAACCAGACCAAAAGTGAGATAAATAAAACAGTCGGTTTCAAAAAAGAGCGTATTGAAAAACCTGATGCCAACAAGTTTaaaa                                                                               >  1:4909357/1‑149 (MQ=255)
                                                                          aacaacTTTTGAATAGAGTCGATTAAAACTGACTGTGTTCCCCCGAGGATCAACCCCACCGCCGTAACCACACCAAAAGTGAGATAAATAAAACACTACGTTTCAAAAAAGAGCGGATTGAAAAAACTGATGCCAACAAGTTTAaacaaa                                                                            >  2:7747789/1‑149 (MQ=255)
                                                                             aacTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGAACACCCCCAGCGCGGTAAACAGACCAAACGAGAGATAAAAAAACCACTCGGTTGAAAAAAAAAACCAATTGAAAAAAGTGATTCCAAAAACTTTAaacaacaaa                                                                         >  2:257346/1‑147 (MQ=255)
                                                                                ttttGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCAAAAGTGAGATAAAAAAACCAGTCGGTTTCAAAAAAGAGCGTATTGTAAAACGTGATGCCAACAAGTTTaaaaaaataacac                                                                      >  2:7529128/1‑143 (MQ=255)
                                                                                       tAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCAAACCTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTAAAAAAAGTGATGCCAACAACTTTAAACAACACACACCACACTa                                                               >  2:629151/1‑150 (MQ=255)
                                                                                         ttgTCGATTAAAGGTTAGTGTGTTGGCGCTTGGAACACCGCCTGCGCTGTAACCTCACCTAAGTTTATATATATAAACCAGTCGTTTTAAAAAAAGTTCGTATTTAAAATGGTTATGCAAACATTTTTAAACAACTCACTCCACAGTaaa                                                             <  1:6579443/148‑1 (MQ=255)
                                                                                         ttgTCGATTAAAGGTTACTGTGTGCGCGCGTGTATCACCGCCAGCGCGGTAACCAGACCTACCGTGATATAAAAAAACCAGTGGGTTGAAAAAAATAGCGTATTTAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTaaa                                                             <  1:3458181/148‑1 (MQ=255)
                                                                                         gtgTCGTTTAAACGTGAGTGTGTTCGCGCGTGGATCACCGCCAGCGGGGTAACCAGACCTATCGTGAGATAAATAAACCTGTCGGTTGCAAAAAAGAGCGTTTTGAAAAAGGTGATGCCAACATGTTTAAACAACTCACTCCACAGTaaa                                                             <  1:945868/148‑1 (MQ=255)
                                                                                          agTCTATTAAACGTGACTGTGTTCGCGCGAGGATCCCCGCCTGCGCGGTAACCAGACCTAACTTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAATTTTAAACAACTCACTCCACAGTaaaa                                                            <  1:7770858/150‑1 (MQ=255)
                                                                                            tCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGc                                                          >  1:5113349/1‑150 (MQ=255)
                                                                                            tCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGc                                                          >  1:7712588/1‑150 (MQ=255)
                                                                                              gATTAAACGTGAGTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTACCGTTAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAc                                                        <  1:8487679/150‑1 (MQ=255)
                                                                                              gATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAc                                                        <  2:4171458/150‑1 (MQ=255)
                                                                                               aTTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGACGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAcc                                                       >  1:3132084/1‑150 (MQ=255)
                                                                                                ttAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCa                                                      <  1:1309201/150‑1 (MQ=255)
                                                                                                ttAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCa                                                      >  2:82776/1‑150 (MQ=255)
                                                                                                ttAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCa                                                      <  2:3994577/150‑1 (MQ=255)
                                                                                                 tAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAg                                                     >  1:5719099/1‑150 (MQ=255)
                                                                                                     cGTGAGTGTGTTCGCGCGAGGAACACCGCCAGCGCGGTAACCAGACCTAACGTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCAc                                                 <  1:7350497/150‑1 (MQ=255)
                                                                                                     cGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCAc                                                 >  1:3261700/1‑150 (MQ=255)
                                                                                                       tGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGt                                               <  2:5310062/150‑1 (MQ=255)
                                                                                                        gACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTc                                              <  2:6158610/150‑1 (MQ=255)
                                                                                                         aCTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCa                                             >  1:5570829/1‑150 (MQ=255)
                                                                                                          cTGTGTTCGCGCGAGGATCACCGCCAGCGCTGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCaa                                            <  1:5387522/150‑1 (MQ=255)
                                                                                                               ttCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCAt                                       >  1:3709229/1‑150 (MQ=255)
                                                                                                                tCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCAtt                                      <  1:6939608/150‑1 (MQ=255)
                                                                                                                  gcgcgAGGATCACCGCCAGCGCGGTAACCATACCTAACGTGAGTTAAATAAACCTGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTgg                                    <  1:7393438/150‑1 (MQ=255)
                                                                                                                   cgcgAGGATCACCGCCAGCGCGGTACCCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGc                                   <  1:2584632/150‑1 (MQ=255)
                                                                                                                     cgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCga                                 <  2:6854412/150‑1 (MQ=255)
                                                                                                                     cgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCga                                 <  2:2277680/150‑1 (MQ=255)
                                                                                                                      gAGGATCACCGCCAGCGCGGTAACCAGACCTACCGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCgag                                <  1:4168865/150‑1 (MQ=255)
                                                                                                                      gAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCgag                                >  1:2244921/1‑150 (MQ=255)
                                                                                                                       aGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCgaga                               >  2:1225720/1‑150 (MQ=255)
                                                                                                                        ggATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaa                              <  2:872836/150‑1 (MQ=255)
                                                                                                                          aTCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCACTGGCGAGaaaa                            >  2:724913/1‑150 (MQ=255)
                                                                                                                           tCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaaaaa                           >  2:2801167/1‑150 (MQ=255)
                                                                                                                              ccGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACCGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCa                        >  1:1904255/1‑150 (MQ=255)
                                                                                                                              ccGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCa                        <  2:4564703/150‑1 (MQ=255)
                                                                                                                              ccGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCa                        >  2:368331/1‑150 (MQ=255)
                                                                                                                                 ccAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCa                     >  2:3135483/1‑150 (MQ=255)
                                                                                                                                  cAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                    <  2:6798369/150‑1 (MQ=255)
                                                                                                                                  cAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTAAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                    <  1:629151/150‑1 (MQ=255)
                                                                                                                                     cgcgGTAACCAGACCTAACGTGATATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTaa                 <  1:3840922/150‑1 (MQ=255)
                                                                                                                                     cgcgGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTaa                 <  1:6789808/150‑1 (MQ=255)
                                                                                                                                     cgcgGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTaa                 <  1:5062683/150‑1 (MQ=255)
                                                                                                                                      gcgGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAat                <  1:4763882/150‑1 (MQ=255)
                                                                                                                                              cAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTAc        >  1:2084654/1‑150 (MQ=255)
                                                                                                                                               aGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACg       >  1:5803741/1‑150 (MQ=255)
                                                                                                                                                   cTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTAt   >  1:5929259/1‑150 (MQ=255)
                                                                                                                                                   cTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTAt   <  2:4404948/150‑1 (MQ=255)
                                                                                                                                                   cTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTAt   >  1:839726/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGTCGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATG  >  NC_000913/3531725‑3532022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑