Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,462 T→A 6.2% intergenic (+17/+70) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4620TA 6.2% 1148.9 / 7.8 395intergenic (+17/+70)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (83/252);  new base A (31/2);  total (140/255)
Fisher's exact test for biased strand distribution p-value = 2.08e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 403 total aligned reads displayed.

TCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAG  >  NC_000913/3959313‑3959611
                                                                                                                                                     |                                                                                                                                                     
tCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCt                                                                                                                                                       <  2:7581740/150‑1 (MQ=255)
  cGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCcaca                                                                                                                                                     >  2:4707106/1‑150 (MQ=255)
     aaGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAccc                                                                                                                                                  <  2:5918065/150‑1 (MQ=255)
        gcgcGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGa                                                                                                                                               <  2:8394709/150‑1 (MQ=255)
          gcgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAcc                                                                                                                                             <  2:1309508/150‑1 (MQ=255)
            ggTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCct                                                                                                                                           >  1:5106530/1‑150 (MQ=255)
             gTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAc                                                                                                                                                    >  2:6119381/1‑140 (MQ=255)
             gTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCctc                                                                                                                                          <  1:6800057/150‑1 (MQ=255)
                gATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTcc                                                                                                                                       <  1:1820027/150‑1 (MQ=255)
                  tAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCGTCACCCCGACCCtctcact                                                                                                                                     <  2:2084514/150‑4 (MQ=255)
                  tAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCca                                                                                                                                     >  1:1416793/1‑150 (MQ=255)
                  tAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCTCCca                                                                                                                                     >  2:5634883/1‑150 (MQ=255)
                   aaCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcac                                                                                                                                    <  1:6462933/150‑1 (MQ=255)
                    aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                   <  1:7695299/150‑1 (MQ=255)
                    aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                   <  1:5327015/150‑1 (MQ=255)
                     cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                                  <  1:7765249/150‑1 (MQ=255)
                     cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                                  <  1:2449579/150‑1 (MQ=255)
                     cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                                  <  1:5432652/150‑1 (MQ=255)
                      gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagg                                                                                                                                 <  2:5150317/150‑1 (MQ=255)
                        gCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACaggga                                                                                                                               <  1:2962845/150‑1 (MQ=255)
                           aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagag                                                                                                                            <  1:4046873/150‑1 (MQ=255)
                           aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagag                                                                                                                            <  1:8258222/150‑1 (MQ=255)
                              gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                         <  1:2243030/150‑1 (MQ=255)
                               cGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagggag                                                                                                                        <  2:7320409/150‑1 (MQ=255)
                                 tGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaa                                                                                                                      <  1:1474615/150‑1 (MQ=255)
                                   aTGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaaa                                                                                                                    <  1:4684594/150‑1 (MQ=255)
                                      tgAACGACGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAacac                                                                                                                 <  1:5316798/150‑1 (MQ=255)
                                      tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTATGTGCGCGATGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAacac                                                                                                                 <  1:3773482/150‑1 (MQ=255)
                                      tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGCGAAAacac                                                                                                                 >  2:2027190/1‑150 (MQ=255)
                                      tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAacac                                                                                                                 <  2:3334566/150‑1 (MQ=255)
                                      tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACACCCACCGGGCGGGGGGGAAAaaac                                                                                                                 >  2:4099553/1‑150 (MQ=255)
                                        aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                               <  1:4434785/150‑1 (MQ=255)
                                         aCGTAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCa                                                                                                              <  2:7930084/150‑1 (MQ=255)
                                            aaGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCCCCCACTCCCCCCGGGCGCGGGCGACAACACACCAgg                                                                                                           >  1:7380803/1‑150 (MQ=255)
                                             aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                          <  2:3095836/150‑1 (MQ=255)
                                                gATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCtt                                                                                                       <  2:3498696/150‑1 (MQ=255)
                                                  ttCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtct                                                                                                     <  2:2511749/150‑1 (MQ=255)
                                                   tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtctc                                                                                                    <  2:3827538/150‑1 (MQ=255)
                                                        gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                               <  2:2781499/150‑1 (MQ=255)
                                                        gCCATGCGAGTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                               <  1:7187570/150‑1 (MQ=255)
                                                           aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                            >  2:1426683/1‑150 (MQ=255)
                                                           aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                            <  1:45421/150‑1 (MQ=255)
                                                           aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                            <  2:39707/150‑1 (MQ=255)
                                                            tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAg                                                                                           <  1:3540747/150‑1 (MQ=255)
                                                              cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                         <  1:8195135/150‑1 (MQ=255)
                                                              cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                         <  1:8670906/150‑1 (MQ=255)
                                                               gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGcc                                                                                        <  1:2820720/150‑1 (MQ=255)
                                                               gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGcc                                                                                        <  1:4609218/150‑1 (MQ=255)
                                                               gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCCCAGGGTGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGTAGGcc                                                                                        >  1:4938484/1‑150 (MQ=39)
                                                               gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACAACCACAGGGGGTGGGAGAAAAAACACAAGGCCTTCTACAGGAGAAGGcc                                                                                        >  1:1706460/1‑150 (MQ=255)
                                                               gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACAACCACAGGGAGAGGGCGAAAAAACGAAAGGCCATATCCAGGAGAAGGcc                                                                                        >  1:5577341/1‑150 (MQ=255)
                                                                agtGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAACCGTAGTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCGGGAGAAGGCCt                                                                                       <  2:7176451/148‑1 (MQ=255)
                                                                aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCCCCCCCCCCCACAACCCCACGGGGAGGGGGAAAAAAAAAAAAGCCATCTACAGGAGAAGGcca                                                                                       >  1:5184531/1‑149 (MQ=255)
                                                                 ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCtt                                                                                      <  1:6044317/150‑1 (MQ=255)
                                                                        ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAcc                                                                                                                                             >  1:7576951/1‑88 (MQ=255)
                                                                        ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTgagtgtt                                                                               <  1:7052155/150‑7 (MQ=255)
                                                                            ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                                           >  1:8375544/1‑150 (MQ=255)
                                                                             gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                                          <  2:8348178/150‑1 (MQ=255)
                                                                              taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGAACCCCGACCCTCTCCCACCCGGCGAGGGAGAAAACACACAATGCCGTATCCGGGAGAAGGCCGGGCGATTATTTGCg                                                                         >  2:7065611/1‑150 (MQ=255)
                                                                              taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCGAACCCCCAGGGGGAGGGAGAAAAAAAACAAGGCCTTCGCCGGGAGAAGGCCGTGGTATTAGTTTCg                                                                         >  2:6693330/1‑150 (MQ=255)
                                                                               aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                        <  1:3682206/150‑1 (MQ=255)
                                                                                 gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                      <  2:374085/150‑1 (MQ=255)
                                                                                 gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCCCCCCAACCCCCAGCGAGGGGGCGAAAACACTCAAGGCCATCTCCGGGGGAAGGGCGTGCGATTATTTgggggt                                                                      >  1:1604526/1‑148 (MQ=255)
                                                                                     cggcgcGGCTATATGACAGATATGAACCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                                                  <  2:3605280/148‑1 (MQ=255)
                                                                                      tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACCGGGAGAGGGAGAAAACACTCAAGGCCGTCAACTGGAGAACGCCTTGCGAGTAGTTGCGGTACCGcc                                                                 >  2:4538730/1‑149 (MQ=37)
                                                                                      tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGGTACGTGCGCGCTGATGCCCGCACCCCGCCCCGCTCCCCCCGGGGGAGGGCGAAAACACGCAAGGCCGTCTCCCGGCGGAGGCCGGTCTATTTTTTGCGGGACAGcc                                                                 >  2:110342/1‑149 (MQ=255)
                                                                                        cgcgGCTATATGACAGATATGAACCGTATTGCTGTTGCGGGTTAAGTGCGCGCGGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTCGTTGCGGTACAGCAcc                                                               <  2:1000638/150‑1 (MQ=255)
                                                                                         gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGTGAAAACACTCAAGGCCTTCTCCTGGAGAAGGGCGTGCTATTAGTTGCGGTACAGCTCCt                                                              >  1:6521264/1‑150 (MQ=38)
                                                                                             cTATATGACAGAGATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                          <  1:8624074/150‑1 (MQ=255)
                                                                                                   gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCtt                                                             <  1:5780597/141‑1 (MQ=255)
                                                                                                   gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCCCCCCCACCCACACCCCCCGGGGCAGGGGGAAAAAACCCAACGCCATCACCGGGCGAAGGCCGGGCGATTAGGTGCGGGCCAGCCCCATCATGAggtg                                                    >  2:1702682/1‑150 (MQ=255)
                                                                                                    aCAGATCTGAAACGTATGGCTGTTGCGGGTTAAGTGCGCGCGGATGCCCGCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCTGGAGAAGGCCGTGCTATGAGTTGCGGTACAGCACCTTAATGATGTGa                                                   <  2:437056/150‑1 (MQ=38)
                                                                                                     cAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat                                                  <  2:6179444/150‑1 (MQ=255)
                                                                                                      aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCAACCAAAACCACAGGGCGAGGGGGAAAACAAACAAGGCCATCACCTGGAGAAGGCCATTCTAATAGTTGCGGTAAAGCACCATAATGATTTGata                                                 >  2:6017038/1‑150 (MQ=255)
                                                                                                         tatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                                              <  1:4715390/150‑1 (MQ=255)
                                                                                                             aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCACACCCCCCCCCTATCCCCCCCGGGGCGGGGGGAAACACGCCAGGCCTTCTCCGGGCGCAGGCCGTGCGATTAGTTGCGGTACAGCCCCGTAATGGTGGGGTATCCGAAc                                          >  2:7505614/1‑150 (MQ=255)
                                                                                                             aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGCGAGGGCGAAAACACTCAAGGCCATCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAc                                          >  2:4325544/1‑150 (MQ=255)
                                                                                                             aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTAACCCCGACACTCTCCCACACGGTGAGGGTGAAAACACGCAAGGCCTTCTGCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAAc                                          >  2:5548863/1‑150 (MQ=255)
                                                                                                                 gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCTCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                                      >  2:6462464/1‑150 (MQ=255)
                                                                                                                  tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                     <  1:6218988/150‑1 (MQ=255)
                                                                                                                  tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                     <  1:7834718/150‑1 (MQ=255)
                                                                                                                  tATTGCTGTTGCGGGGTAAGTGCGCGCTGATGCCCTCACCCCGACCCTATCCCACAGGGAGAGGGAGAAAAAACACAAGGCCGTCTCCTGGGGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGGACTGGgt                                     >  2:5724387/1‑150 (MQ=38)
                                                                                                                    gatcTGTGGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                                   <  1:4179190/147‑1 (MQ=255)
                                                                                                                       cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa                                <  2:6853957/150‑1 (MQ=255)
                                                                                                                         gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc                              <  2:5412758/150‑1 (MQ=255)
                                                                                                                          ttGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACAGGGCCAGGGAGAAAACACTCAACGCCATCGCCGGGAGAAGGCCGTGCTAATAGTTGCGGTACAGCACCATAAAGATGTGATATCCCAACAGGGTGGGcgatc                             >  2:4179190/1‑146 (MQ=255)
                                                                                                                          ttGCGGGGTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCGGGCGGAGGCCGTGCTATTAGTTGCGGGACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                             >  2:7974726/1‑150 (MQ=38)
                                                                                                                           tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg                            <  1:6614677/150‑1 (MQ=255)
                                                                                                                             cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                          <  1:4321255/150‑1 (MQ=255)
                                                                                                                             cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                          <  2:5090213/150‑1 (MQ=255)
                                                                                                                             cGGGTTAAGTGCGCGCTGATGCCCGCAACCCCACCCACCACCCCACGGAGCGGGAGAAAACACACAAAGCCATCTCCGGGAGAAGGCCGTGCTATTAGTTTCGGGAAACCACCCTAATGATTTGATATACCAACACGGTGTGCAGCGGGc                          >  2:8169192/1‑150 (MQ=255)
                                                                                                                               ggTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCACCCACAGGGAGAGGGAGAAAACACACAAGGCCTTCTACTGGATAAGGCCGTGCTATTAGTTGCGGTACACCACCTTAATTATGTGATATACGAACTGGGTGTTCAGCGGGCCg                        >  2:7818319/1‑150 (MQ=37)
                                                                                                                               ggTTAAGTGCGCGCTGATGCCCACACCCCCACCCACAACCACAGGGCGAGGGAGAAAACACAAAAGGCCTTCACCAGGGGAAGGCCTTGCGATTAGTTGCGGTACAGCACCAAAAAGATGTGATAAACCAACAGGGTGTTCCCCGGGCCg                        >  2:426339/1‑150 (MQ=255)
                                                                                                                                  tAAGTGCGCGCTGATGCCCGCAACCCGAACCACTACCCCAGGGAGAGGGAGAAAAAAAGCAACGCCATATCCTGGAGAAGGCCGTGCTATTATTTGCGGTAAAGCACCTTAATGATGTGTTATACGAACAGGGTGTGCAGCCGGCCCGTc                     >  2:4909828/1‑150 (MQ=255)
                                                                                                                                    aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                   <  1:1011507/150‑1 (MQ=255)
                                                                                                                                         gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa              <  2:6734906/150‑1 (MQ=255)
                                                                                                                                         gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa              <  1:3286916/150‑1 (MQ=255)
                                                                                                                                              gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc         <  2:2669281/150‑1 (MQ=255)
                                                                                                                                                   ccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACa    <  2:2248607/150‑1 (MQ=255)
                                                                                                                                                    cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACaa   >  2:2888659/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAG  >  NC_000913/3959313‑3959611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: