Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,526 T→G 6.8% intergenic (+81/+6) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5260TG 6.8% 1262.6 / 2.6 401intergenic (+81/+6)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (169/183);  new base G (38/3);  total (215/186)
Fisher's exact test for biased strand distribution p-value = 8.75e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 412 total aligned reads displayed.

ATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCC  >  NC_000913/3959377‑3959675
                                                                                                                                                     |                                                                                                                                                     
 ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCtt                                                                                                                                                      <  2:3614659/150‑1 (MQ=255)
   gAGCAGGTAGGTAAGACACTGCGCGGCTATATTACAGATATTAACCGTATTTCTTTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGACTTATCCTGTAGAAGGCGTTGc                                                                                                                                                    <  2:5008880/150‑1 (MQ=37)
   gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACACCCCCAGGGAGAGGGAGAAAAAACTCAAGGCCTTCTCCTGGAGAACGCCGTGc                                                                                                                                                    >  2:1024831/1‑150 (MQ=39)
   gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTAACCCCCACCCGCAACCCCCGGGAGAGGGAGAAAACAAAAAAGGCCTTATCCTGGTGAAGGCCTTgg                                                                                                                                                    >  1:4692317/1‑149 (MQ=255)
     gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa                                                                                                                                                  <  2:2040231/150‑1 (MQ=255)
        ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTgagtgtt                                                                                                                                               <  1:7052155/150‑7 (MQ=255)
        ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAACCCGACCCACACCCACACGGAGAGGGAGAAAACAAACAAGGCCTTATCCGGGAGAAGGCCCTGCTAATa                                                                                                                                               >  2:4233630/1‑150 (MQ=255)
              taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGAACCCCGACCCTCTCCCACCCGGCGAGGGAGAAAACACACAATGCCGTATCCGGGAGAAGGCCGGGCGATTATTTGCg                                                                                                                                         >  2:7065611/1‑150 (MQ=255)
               aaGAAACTGCGCGGCTATATGACAGATATGAAACGGATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCCCCCTCTCCCACACGGCGAGGGAGAAAACACACAAGGCCATCAACGGGAGAAGGCCGTGCGAATAGTTTCgg                                                                                                                                        >  2:7542123/1‑150 (MQ=255)
                 gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCCCCCCAACCCCCAGCGAGGGGGCGAAAACACTCAAGGCCATCTCCGGGGGAAGGGCGTGCGATTATTTgggggt                                                                                                                                      >  1:1604526/1‑148 (MQ=255)
                   aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                                                                                                    <  2:3155883/150‑1 (MQ=255)
                      tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCGACCCTCAACCACAGGGAGAGGGAGAAAACAATCAATGCCATATCCTGGAGAAGGCCGAGGTATTAGTTGCGGTgcagca                                                                                                                                 >  1:5103722/1‑150 (MQ=37)
                         gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCAAAACCACAGGGCGAGGGGGAAAAAACACAACGCCTACTCCTGGGGGAGGGCGGGCTATTAGTTTGGGTACAGCaacg                                                                                                                              >  1:6835395/1‑147 (MQ=255)
                           ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGGGAGGGCGAAAACACTCAAGGCCTTCTCCTGGAGTAGGCCTTGATATTAGTTGCGGGACCGCACCTTa                                                                                                                            >  2:7470607/1‑150 (MQ=38)
                            gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                                                                                                           <  1:3317679/150‑1 (MQ=255)
                             cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                                                                                          <  1:5200595/150‑1 (MQ=255)
                              tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACTCCCACAGGGGGAGGGAGAAAACACACAAAGCCATATCCAGGAGAAGGCCTTGCTATTATTTGCGGTACAGCAccgaaatt                                                                                                                         >  1:8344731/1‑144 (MQ=37)
                                 atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                                                                      <  2:3444597/150‑1 (MQ=255)
                                 atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                                                                      <  1:5525713/150‑1 (MQ=255)
                                  tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACAACCCCACCCACTCCCCCAGGGAGAGGGAGAAAACACACAACGCCGAATCCAGGAGAAGGGCTTGCTAATAATTTCGGTAAAGCACCATaaatatgg                                                                                                                     >  2:6731432/1‑144 (MQ=255)
                                    aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                                                                   <  2:4270494/150‑1 (MQ=255)
                                     cAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCCCCCACAACCACCGGGGGGGGGAGAAAACACACAAGGCCGTCTCCGGGTGAAGGCCGGGCGATTAGTTTCGGTACAGCACCATAATGTtgtggt                                                                                                                  >  2:8105113/1‑148 (MQ=255)
                                      aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCAACCAAAACCACAGGGCGAGGGGGAAAACAAACAAGGCCATCACCTGGAGAAGGCCATTCTAATAGTTGCGGTAAAGCACCATAATGATTTGata                                                                                                                 >  2:6017038/1‑150 (MQ=255)
                                        atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                                                               <  1:3623930/150‑1 (MQ=255)
                                         tatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                                                                                                              <  1:4715390/150‑1 (MQ=255)
                                           tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                                                                                                            <  1:1431437/150‑1 (MQ=255)
                                             aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATTCCCTACCCCCCACCCTCTCCAACAGGGAGAGCGAGAAAACACTCAAGGCCTTATCCTGGAGAAGGCCATGCTATTAGTTGAGGGACAGCACCATAATGAAGTGATATCCGAAc                                                                                                          >  2:8596023/1‑150 (MQ=255)
                                              aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGGGAGGGAGAAAACACTCAAGGCCATCGCCTGGAGAAGGCCTTGCGATTAGTTGCGGTAAAGCACCATAATGATGTGTTATCCGAAcg                                                                                                         >  2:5247306/1‑149 (MQ=38)
                                                cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTTCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                                       <  2:6613797/150‑1 (MQ=255)
                                                cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                                       <  1:7713732/150‑1 (MQ=255)
                                                cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                                       <  2:1685358/150‑1 (MQ=255)
                                                  tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                                                                     <  1:5721772/150‑1 (MQ=255)
                                                  tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                                                                     <  2:5110333/150‑1 (MQ=255)
                                                  tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                                                                     <  1:7834718/150‑1 (MQ=255)
                                                  tATTGCTGTTGCGGGGTAAGTGCGCGCTGATGCCCTCACCCCGACCCTATCCCACAGGGAGAGGGAGAAAAAACACAAGGCCGTCTCCTGGGGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGGACTGGgt                                                                                                     >  2:5724387/1‑150 (MQ=38)
                                                    gatcTGTGGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                                                                                                   <  1:4179190/147‑1 (MQ=255)
                                                          tttcGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGTGGGAGAAAACACTCTAGGCCTTCTCCTGGAGAAGGCCTTGATATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                                             <  1:4417415/147‑1 (MQ=255)
                                                          ttGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACAGGGCCAGGGAGAAAACACTCAACGCCATCGCCGGGAGAAGGCCGTGCTAATAGTTGCGGTACAGCACCATAAAGATGTGATATCCCAACAGGGTGGGcgatc                                                                                             >  2:4179190/1‑146 (MQ=255)
                                                           tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg                                                                                            <  1:6614677/150‑1 (MQ=255)
                                                             cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                                                                          <  1:5634883/150‑1 (MQ=255)
                                                               ggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCCCGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg                                                                                        <  2:4331535/150‑1 (MQ=255)
                                                                gTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGCGAGGGAGAAAACACACAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGGGGGCCgg                                                                                       >  2:5422039/1‑150 (MQ=255)
                                                                  tAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAACACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                                                                                     <  1:3813357/150‑1 (MQ=255)
                                                                    aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                                                   <  1:1011507/150‑1 (MQ=255)
                                                                       gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                                                                <  1:2869879/150‑1 (MQ=255)
                                                                       gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                                                                <  1:1730459/150‑1 (MQ=255)
                                                                           gcTGATGCCCACACCCCCACCCACACCCACCGGGAGAGGGCGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTTCTAATAGTTTCGGTACACCACCATAATTATGTGATAAACGAACGGGGTGTGCCGCGGGCCGGTCGGCTCCAGt                                                                            >  2:8398980/1‑150 (MQ=255)
                                                                                   ccTCCCCCCGACCCTCTCCCACAGGGAGCGGGAGAAAACACTCAAGGCCTTCGCCGGGAGAAGGCCGGGCGATTAGGTGCGGGACAGCACCTGCAGGATGGGATAGCCGAACGGGGTGTGCCGCGGGCCGGTCGGCTCCAGTACCGGACa                                                                    >  2:7169796/1‑150 (MQ=255)
                                                                                    cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACaa                                                                   >  2:2888659/1‑150 (MQ=255)
                                                                                     tCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAag                                                                  <  2:3584459/150‑1 (MQ=255)
                                                                                     tCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAag                                                                  >  2:4005803/1‑150 (MQ=255)
                                                                                       aCCCCGACCCTCTCCCACAGGGAGATGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAagag                                                                <  1:4233630/150‑1 (MQ=255)
                                                                                       aCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAagag                                                                <  2:3706849/150‑1 (MQ=255)
                                                                                         cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                                                              >  2:1979754/1‑150 (MQ=255)
                                                                                         cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                                                              <  1:4470246/150‑1 (MQ=255)
                                                                                          ccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                                       >  2:6478375/1‑108 (MQ=255)
                                                                                           cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                                            <  1:874015/150‑1 (MQ=255)
                                                                                           cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                                            <  1:3510453/150‑1 (MQ=255)
                                                                                           cGACCCTCGCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTAGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                                            <  2:1901431/150‑1 (MQ=255)
                                                                                              cccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa                                                         <  1:5966746/150‑1 (MQ=255)
                                                                                               ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc                                                        >  1:7474413/1‑150 (MQ=255)
                                                                                               ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc                                                        >  1:7290551/1‑150 (MQ=255)
                                                                                                ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt                                                       >  2:4622542/1‑150 (MQ=255)
                                                                                                     ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                                                  >  2:7875641/1‑150 (MQ=255)
                                                                                                        caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa                                               <  2:6289219/150‑1 (MQ=255)
                                                                                                         agggagagggagAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAAc                                              >  1:1233650/1‑150 (MQ=255)
                                                                                                          gggagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACg                                             >  1:1899929/1‑150 (MQ=255)
                                                                                                           tgagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGc                                            >  2:1318093/2‑150 (MQ=255)
                                                                                                           ggagtgggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGc                                            <  1:6157590/150‑1 (MQ=255)
                                                                                                           ggagagggagaAAACACTCTAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATCTCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTATAGCGAACGc                                            >  2:5830422/1‑150 (MQ=255)
                                                                                                            gagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGcc                                           >  2:1861193/1‑150 (MQ=255)
                                                                                                             agagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCg                                          >  2:2213932/1‑150 (MQ=255)
                                                                                                              gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                                         >  1:137108/1‑150 (MQ=255)
                                                                                                              gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                                         >  2:2439094/1‑150 (MQ=255)
                                                                                                              gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                                         >  2:6879076/1‑150 (MQ=255)
                                                                                                                gggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGaa                                       <  1:6514858/150‑1 (MQ=255)
                                                                                                                    gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                                   >  2:2720531/1‑150 (MQ=255)
                                                                                                                    gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                                   >  2:6820378/1‑150 (MQ=255)
                                                                                                                    gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                                   >  1:4809605/1‑150 (MQ=255)
                                                                                                                    gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                                   >  1:6013342/1‑150 (MQ=255)
                                                                                                                    gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                                   <  2:1517484/150‑1 (MQ=255)
                                                                                                                    gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                                   >  2:4519688/1‑150 (MQ=255)
                                                                                                                     aaaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGTACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                                  <  1:5232848/150‑1 (MQ=255)
                                                                                                                       aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACCGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTCCCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg                                >  2:8329547/1‑150 (MQ=255)
                                                                                                                       aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg                                >  1:2649287/1‑150 (MQ=255)
                                                                                                                        acacTCACTGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGa                               <  2:7308348/150‑1 (MQ=255)
                                                                                                                          acTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGAcc                             >  1:3703806/1‑150 (MQ=255)
                                                                                                                            tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCCGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt                           >  1:2085017/1‑150 (MQ=255)
                                                                                                                            gcccggCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt                           <  1:8065790/146‑1 (MQ=255)
                                                                                                                              aaGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTgg                         >  2:3807755/1‑150 (MQ=255)
                                                                                                                               cggCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGc                        <  1:6730239/149‑1 (MQ=255)
                                                                                                                                   cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa                    <  1:426407/150‑1 (MQ=255)
                                                                                                                                    ttCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAAt                   >  1:2486515/1‑150 (MQ=255)
                                                                                                                                       tcCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCa                >  1:6964123/1‑150 (MQ=255)
                                                                                                                                        ccTGGTGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGATCTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACAATATGACCCTGGCGTAATTCAc               <  2:6201037/150‑1 (MQ=255)
                                                                                                                                        ccTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAc               <  2:555933/150‑1 (MQ=255)
                                                                                                                                         cTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAcc              >  1:7888546/1‑150 (MQ=255)
                                                                                                                                           ggAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTa            <  2:4692317/150‑1 (MQ=255)
                                                                                                                                                 ggCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCAc      <  1:8446798/150‑1 (MQ=255)
                                                                                                                                                    cTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCAccgc   >  1:3088625/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCC  >  NC_000913/3959377‑3959675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: