Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,554 G→T 14.5% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5540GT14.5% 1314.8 / 83.1 432intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (52/308);  new base T (69/3);  total (121/311)
Fisher's exact test for biased strand distribution p-value = 1.67e-42
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 438 total aligned reads displayed.

TATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATAA  >  NC_000913/107406‑107703
                                                                                                                                                      |                                                                                                                                                     
tatGTGACCTGCCAGGACGACGCTGACCTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGGACTGGCATAACGACATTTATCCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGa                                                                                                                                                      <  1:5557680/150‑1 (MQ=255)
  tgtgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAAt                                                                                                                                                    >  2:2187772/1‑150 (MQ=255)
   gtgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTTG‑‑TTTTTTTTTGAAtt                                                                                                                                                   <  1:3970558/150‑1 (MQ=255)
    tgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTTTAttt                                                                                                                                                  >  1:7054626/1‑150 (MQ=255)
    tgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTTTAttt                                                                                                                                                  >  1:5702894/1‑150 (MQ=255)
     gACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAAtttt                                                                                                                                                 <  1:1610371/150‑1 (MQ=255)
     gACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAAtttt                                                                                                                                                 <  2:297066/150‑1 (MQ=255)
       ccTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTtat                                                                                                                                               <  2:5760127/150‑1 (MQ=255)
           ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAaga                                                                                                                                           <  1:8595381/150‑1 (MQ=255)
            cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAagag                                                                                                                                          <  1:2011150/150‑1 (MQ=255)
             cggACGACGCAGAACTGCCGTTGGCCTTCAACGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAAGTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGc                                                                                                                                         <  2:8091120/149‑1 (MQ=255)
             aGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTTTATTTTTTAataga                                                                                                                                         >  2:4147784/1‑149 (MQ=255)
                  gacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATaa                                                                                                                                    <  1:4819180/150‑1 (MQ=255)
                  gacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATaa                                                                                                                                    <  1:4696749/150‑1 (MQ=255)
                   acgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACACTCTCCGGCCTGGTGAACCAGTCT‑‑TTTTTTTTTGAATTTTATAAGAGCTATaaa                                                                                                                                   <  2:2304517/150‑1 (MQ=255)
                   acgCAGAACTGCCGTTGGCCTTCAAAGCGACTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATaaa                                                                                                                                   <  2:5512923/150‑1 (MQ=255)
                     gCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGTATTTTATAATAGCTATaaaaa                                                                                                                                 >  2:616042/1‑150 (MQ=255)
                     gCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTTTATTTTTTTATATCTATaaaaa                                                                                                                                 >  1:2312838/1‑150 (MQ=255)
                         aaCTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                                                                                             <  2:1119858/150‑1 (MQ=255)
                            tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGTATTTTAAAAGAGCTATAAAAAAACGTGc                                                                                                                          >  2:8318675/1‑150 (MQ=255)
                            tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                                                                                          <  2:1989824/150‑1 (MQ=255)
                                gTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTTAATTTTATAAATTCAATAAAAAAAAGTGCGAAc                                                                                                                      >  2:9053236/1‑150 (MQ=255)
                                gTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTTTATTTTTTAATATAAATAAAAAAAACTGCGaaa                                                                                                                      >  2:1611747/1‑149 (MQ=255)
                                   ggCCTTCAAAGCGCCTTCAGCTTTACTGGAATAACGAAATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCCGGTGAACCTGTCT‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt                                                                                                                   <  1:1756355/150‑1 (MQ=255)
                                   ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt                                                                                                                   <  2:5937395/150‑1 (MQ=255)
                                     ccTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                                                                                 <  2:3683667/150‑1 (MQ=255)
                                      cTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                                                                                <  2:8583019/150‑1 (MQ=255)
                                      cTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTTTATTTTTTAAAATCGATAAAAAAACGGGGGAACGcgggt                                                                                                                >  2:2655649/1‑146 (MQ=255)
                                          cccgcgcCTTCAGCTGTACTGGCATAACGACATTTATCCTGTCGTATAACAGTCGACTGGCAAATCTGGCCCTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGCGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                                                                                            <  1:2555721/147‑1 (MQ=255)
                                          accgcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAATATTCGACTGGCAAATCTGGCACTCTCGCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                                                                                            <  1:1424257/147‑1 (MQ=255)
                                           aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                                           <  1:5985896/150‑1 (MQ=255)
                                           aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                                           <  1:6095531/150‑1 (MQ=255)
                                           aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                                           <  1:759432/150‑1 (MQ=255)
                                            aGCGCCTACAGCTGTACTCGCATAACCACATTTATACTGTCGTATAAAATACCACTGCCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                                                                                          <  1:5300890/150‑1 (MQ=255)
                                             gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCTGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  1:3775090/150‑1 (MQ=255)
                                             gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  2:4595781/150‑1 (MQ=255)
                                             gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  1:1644944/150‑1 (MQ=255)
                                                 cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                                                                                     <  1:1112093/150‑1 (MQ=255)
                                                     aGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                                                                                 <  2:4252460/150‑1 (MQ=255)
                                                       cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                                                                                               <  2:3600132/150‑1 (MQ=255)
                                                       cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                                                                                               <  1:6206287/150‑1 (MQ=255)
                                                        tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCg                                                                                              <  1:8138913/150‑1 (MQ=255)
                                                          tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTTAAATTTATAAGAGCTATAAAAAAACGGGCGTAAGCTGTTTTGTTAAGCACGTTTgcgcc                                                                                            >  2:6858389/1‑149 (MQ=255)
                                                          tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                                                                                            <  1:8340159/150‑1 (MQ=255)
                                                          tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                                                                                            <  2:1834153/150‑1 (MQ=255)
                                                          tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                                                                                            <  1:7552654/150‑1 (MQ=255)
                                                                  tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTTTATTTTTTTAATTGTGTAAAAAACCGGGGGGACGGGGTTTTGGTTAGGACGTTTGCGCACAAcagtt                                                                                    >  2:1263696/1‑146 (MQ=255)
                                                                  tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt                                                                                    <  1:6416353/150‑1 (MQ=255)
                                                                  tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt                                                                                    <  1:9075311/150‑1 (MQ=255)
                                                                  tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt                                                                                    <  1:7138911/150‑1 (MQ=255)
                                                                         aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt                                                                             <  1:414185/150‑1 (MQ=255)
                                                                          tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTc                                                                            <  1:9003934/150‑1 (MQ=255)
                                                                          tttAGACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt                                                                             <  2:7512279/150‑1 (MQ=255)
                                                                               aCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCt                                                                       <  2:4993750/150‑1 (MQ=255)
                                                                                  gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg                                                                    <  2:3871096/150‑1 (MQ=255)
                                                                                    cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGa                                                                  <  1:3251744/150‑1 (MQ=255)
                                                                                    cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGa                                                                  <  1:7900371/150‑1 (MQ=255)
                                                                                     gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAc                                                                 <  2:4438779/150‑1 (MQ=255)
                                                                                         aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCa                                                             <  2:4954800/150‑1 (MQ=255)
                                                                                         aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCa                                                             <  1:6479537/150‑1 (MQ=255)
                                                                                           aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAgg                                                           <  2:5640715/150‑1 (MQ=255)
                                                                                                gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTa                                                      <  1:2899466/150‑1 (MQ=255)
                                                                                                 aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTaa                                                     <  1:5372981/150‑1 (MQ=255)
                                                                                                 aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTaa                                                     <  1:3238453/150‑1 (MQ=255)
                                                                                                  cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAAt                                                    <  1:128729/150‑1 (MQ=255)
                                                                                                  cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTCCATTCGTGCTGTGGACTGCAGGCTTTAAt                                                    <  1:8016233/150‑1 (MQ=255)
                                                                                                   tGGCAAATCTGGCACTCTCTCCGGCCTGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAATCGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATg                                                   <  2:8725031/150‑1 (MQ=255)
                                                                                                   tGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTGAATTTTATAAAATCTATAAAAAAACGTTGGAACGCGGGTTTGTTAATCAAATTTACACACAACCTAAAATCAATCGTGCTGGTGACTGCAGGCGTTTATg                                                   >  2:4715791/1‑150 (MQ=255)
                                                                                                    ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGa                                                  <  2:7094904/150‑1 (MQ=255)
                                                                                                    ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTTTATTTTATAAGAGCTAAAAAAAAACGGGCGGAAGCGGGTTTGTTTAGCACATTTACGGACAACATAACTTAATTAGTGGTGTGGGCTGCAGGCGTTaaagt                                                  >  2:4819294/1‑147 (MQ=255)
                                                                                                     gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTTAAATTTATAAGAGCGATAAAAAAAAGTGGGAACGCAGTTTTATTAATCAAATTTAAGCAAAAAATATATTAATTAGATGTGTGGAAAGCAGGCGTTaaatat                                                 >  2:6796257/1‑146 (MQ=255)
                                                                                                     gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTGTAATTTATAAAAGCTAAAAAAAAAAGTGCGGAAGCTGTTTTATTTATAAAATTTAAGCAAAAAATATAATAAAAAGTTCTGTGGACTGCAGGCATTAATTAt                                                 >  2:8072812/1‑150 (MQ=255)
                                                                                                       aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATaa                                               <  2:2259287/150‑1 (MQ=255)
                                                                                                       aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATaa                                               <  1:2265936/150‑1 (MQ=255)
                                                                                                                aCTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgc                                      <  2:328067/150‑1 (MQ=255)
                                                                                                                aCTCTCTCCGGCCAGGTGAACCAGTCG‑‑TGTTTTTTTTTATTTTTTAATATGTATAAAAAAACGTGCGGACGCGGTTTTGTTAATGAAATTTACGCACAAAATAAATTAAATACTGGGGTTGGATGCAGGCGTTAATGTTAAGATTtttgg                                      >  2:259303/1‑149 (MQ=255)
                                                                                                                 ctctctCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  1:6217103/150‑1 (MQ=255)
                                                                                                                  tctctcCGGCCAGGTGAACCAGTCGTTTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgc                                      <  1:3420938/150‑1 (MQ=255)
                                                                                                                  tctctcCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTTAATTTTATAAGAGCTAAAAAAAAAAGTGGGAACGCGGTTTTATTTATCACATTTAAGCACCAAAAAAATTAAATAGTGGTGTGGGCTGCCGGCTTTAATTTTAATATTTTTtcgc                                    >  2:6572130/1‑150 (MQ=255)
                                                                                                                  tctctcCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  1:6813588/150‑1 (MQ=255)
                                                                                                                    tctcCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTa                                  <  2:3469199/150‑1 (MQ=255)
                                                                                                                         ggCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATAc                             <  1:3148352/150‑1 (MQ=255)
                                                                                                                          gCCTGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACg                            <  2:2114264/150‑1 (MQ=255)
                                                                                                                            cAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGtt                          <  2:9161809/150‑1 (MQ=255)
                                                                                                                            cAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACCtt                          <  2:8374730/150‑1 (MQ=255)
                                                                                                                             aGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                         <  2:5174980/150‑1 (MQ=255)
                                                                                                                             aGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                         <  2:6690752/150‑1 (MQ=255)
                                                                                                                             aGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                         <  2:8902035/150‑1 (MQ=255)
                                                                                                                                tGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa                      <  2:7639559/150‑1 (MQ=255)
                                                                                                                                  aaCCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAata                    <  1:5036849/150‑1 (MQ=255)
                                                                                                                                   aCCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAatat                   <  1:4946242/150‑1 (MQ=255)
                                                                                                                                     cAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGa                 <  1:87838/150‑1 (MQ=255)
                                                                                                                                      aGTCG‑‑TTTTTTTTTGAAATTTATAAGAGCTATAAAAAAAGGTGCGAAAGCTGTTTTCTTAAGCAATTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAATGCAGGCTTTAATTATAAGGTTTTTTCGCTAAAAACGTTTGAAtattat                >  2:5811298/1‑150 (MQ=255)
                                                                                                                                        tCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAATATTTGTGCGCTAAATACGTTTGAATATGATCg              >  2:1665108/1‑150 (MQ=255)
                                                                                                                                          g‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCggg            >  1:4428977/1‑150 (MQ=255)
                                                                                                                                                ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        <  2:1413276/150‑1 (MQ=255)
                                                                                                                                                ttttttGAATTTTATAAGAGCTAAAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        >  2:7723248/1‑150 (MQ=255)
                                                                                                                                                 tttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGc       <  1:4581537/150‑1 (MQ=255)
                                                                                                                                                   tttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACAGCAGCCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCaa     >  2:7011982/1‑150 (MQ=255)
                                                                                                                                                      gAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGGTTGAATATGATCGGGATGGCACTaa  >  2:5323965/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATAA  >  NC_000913/107406‑107703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: