Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 843,216 G→T 9.8% M295I (ATG→ATT rlmF → 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913843,2160GT 9.8% 1329.9 / 26.2 438M295I (ATG→ATTrlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (152/228);  new base T (56/2);  total (208/230)
Fisher's exact test for biased strand distribution p-value = 6.48e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 447 total aligned reads displayed.

CAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTT  >  NC_000913/843067‑843365
                                                                                                                                                       |                                                                                                                                                     
cAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATg                                                                                                                                                       <  1:3340386/150‑1 (MQ=255)
  ggTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGaa                                                                                                                                                     <  1:6475211/150‑1 (MQ=255)
  ggTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCGGGACCTTTATGaa                                                                                                                                                     >  2:6969031/1‑150 (MQ=255)
   gagATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGATGGACGTGGGCGCGGTGAAGGTTGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAac                                                                                                                                                    <  1:1820313/148‑1 (MQ=255)
   gTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGCCCTTTATGAac                                                                                                                                                    >  1:3700579/1‑150 (MQ=255)
   gTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTGCTGGACCGTTATGTac                                                                                                                                                    >  2:82919/1‑150 (MQ=255)
      aTGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAacgac                                                                                                                                                 <  2:2024070/150‑1 (MQ=255)
        gtgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCATTATGAacgacgc                                                                                                                                               >  2:3579359/1‑149 (MQ=255)
         tgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTTTTGGCGGGACCTTTATTAAACACGag                                                                                                                                              >  2:344408/1‑150 (MQ=255)
         tgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCGGGACCGTTATGAACGACGag                                                                                                                                              >  2:415645/1‑150 (MQ=255)
           gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagc                                                                                                                                             >  2:761747/1‑150 (MQ=255)
           gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagca                                                                                                                                            <  2:859174/150‑1 (MQ=255)
           gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTGCTGGACCTTTATGAAAGACGagca                                                                                                                                            >  2:7925846/1‑150 (MQ=255)
                aaTCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGcgcc                                                                                                                                       <  1:7391376/149‑1 (MQ=255)
                 aTCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGcgccg                                                                                                                                      <  2:7166611/150‑1 (MQ=255)
                 aTCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGcgccg                                                                                                                                      <  2:610218/150‑1 (MQ=255)
                    aCTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGccgccg                                                                                                                                   >  2:5347208/1‑150 (MQ=255)
                       ggTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGttt                                                                                                                                <  1:8220954/150‑1 (MQ=255)
                       ggTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTGCAGGACCGTTATTTAACACGAGCACCgccccccatt                                                                                                                                >  1:8540025/1‑146 (MQ=255)
                          aTCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGt                                                                                                                             <  1:4223597/150‑1 (MQ=255)
                          aTCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGGGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGt                                                                                                                             <  1:4169150/150‑1 (MQ=255)
                             tcGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCaa                                                                                                                          >  2:6842740/1‑150 (MQ=255)
                             tcGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTGCCTGGGCCGTTATTAACGACCAGCAGCGCCCCCGGTTTGTCaa                                                                                                                          >  1:7232954/1‑150 (MQ=255)
                              cGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAAt                                                                                                                         <  1:3121740/150‑1 (MQ=255)
                               gtggtgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGGGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATc                                                                                                                        <  1:8361041/150‑1 (MQ=255)
                                   tgAAAACTAACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATTAACGACGACCAGCGCCGCCGGTTTGTCAATAGccc                                                                                                                    >  1:5052127/1‑149 (MQ=255)
                                     aaaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGc                                                                                                                  <  2:7747904/150‑1 (MQ=255)
                                     aaaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGc                                                                                                                  <  1:747715/150‑1 (MQ=255)
                                     aaaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTGCTGGACCATTATTAAAGACGAGCCGCGCCGCCCTTTTGTGAAAAGCCAGc                                                                                                                  >  2:170464/1‑150 (MQ=255)
                                        aCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTCCGGGGCCGTTTTGTACGACGAGCAGCGCCGCCGGGTTGGGAATAGCCcgcggg                                                                                                               >  1:4931546/1‑148 (MQ=255)
                                         cTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCAGGACCATTATTAACCACGAGCCGCGCCCCCGATTTGTCAAAAGCCAGCGTTa                                                                                                              >  1:6018748/1‑150 (MQ=255)
                                          ttACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTaa                                                                                                             >  2:8436728/1‑150 (MQ=255)
                                             ccgccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGc                                                                                                          <  2:674671/150‑1 (MQ=255)
                                              cgccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCAGGGCCGTTATGAACGACGAGCCGCGCCGCCCTTTTGTCAAACGCCCGCCGTAAAGCg                                                                                                         >  1:2901542/1‑150 (MQ=255)
                                               gccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGt                                                                                                        <  1:445021/150‑1 (MQ=255)
                                               gccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCGGGACCTTTATGAACGACGAGCAGCGCCCCCCGTTTGTCAATCGCCAGCGGTAAATCGt                                                                                                        >  2:2241092/1‑150 (MQ=255)
                                                ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                                       <  1:8674456/150‑1 (MQ=255)
                                                ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                                       <  2:4106802/150‑1 (MQ=255)
                                                ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                                       <  2:9129997/150‑1 (MQ=255)
                                                   ttGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTGCAGGACCTTTATTAAAGACGAGCAGCGCCCCCCGTTTTTCAAAAGCCACCCGTAAAACggtggt                                                                                                    >  1:2331796/1‑150 (MQ=255)
                                                   ttGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGt                                                                                                    <  2:4736876/150‑1 (MQ=255)
                                                      tATCGTGCCCTGCCGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTcc                                                                                                 <  2:1230879/150‑1 (MQ=255)
                                                      tATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTcc                                                                                                 <  2:5688267/150‑1 (MQ=255)
                                                       aTCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTccc                                                                                                <  2:3454695/150‑1 (MQ=255)
                                                        tCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGGTATTTCCGGGACCATTATGAACCACCAGCCGCGCCCCCCTTTTGTCAAAACCCCCCCGTAAAGCGGTGGTTcccc                                                                                               >  1:9076562/1‑150 (MQ=255)
                                                        tCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGGTATTGCCGTGGACTTTATGAACGACGAGCCCCGCCGCCCGTTTGTAAATCGCCAGCGGTAAATCGTTGGGTaccc                                                                                               >  1:5548604/1‑150 (MQ=255)
                                                           tGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcgg                                                                                            <  1:1559851/150‑1 (MQ=255)
                                                               ccTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCAGGGCCATAATGTACCAACAGCACCCCCGCCCTGTTGTCAATAGCCAGCGGGAAAGAGTTGGTTGCCCcggcgg                                                                                         >  1:2715544/1‑150 (MQ=255)
                                                               ccTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcggcgg                                                                                         >  2:6143871/1‑150 (MQ=255)
                                                                   acggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCAGGCCCATTATTAACGAAGAGCAGCGCCCCCGTTTTTTCAAAAGCCACCCGTAAATCCGTGGTTACCCCCGCGGTAAc                                                                                     >  1:1392447/1‑150 (MQ=255)
                                                                   acggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTGTATTTGCTGGACCATTATGAAAGACGAGCAGCGCCGCCGTTTTTTCAATAGCCACCGTTAAAGCGTTGGTTACCCCCGCCGTAAc                                                                                     >  2:8813154/1‑150 (MQ=255)
                                                                    cggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTGCCGGGGCCGTTTTTAACCACGAGCAGCGCCCCCGGTTTTTCAAAAGCCACCGGTAAAACCTTGGTTCCCCCCCCGGGAAcc                                                                                    >  1:552332/1‑149 (MQ=255)
                                                                     ggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCATTATGAACCACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCCGCGGTAACgg                                                                                   >  2:7563941/1‑150 (MQ=255)
                                                                     ggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTGCGGGCCCGTTATGGACGACCCGCCGCGCCGCCGGTTTGTCAAACGCCCCCGGTAAAGCGGTGGTTCCCCCCGCGGGAACgg                                                                                   >  2:6519415/1‑150 (MQ=255)
                                                                         gTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCa                                                                               <  1:2197890/150‑1 (MQ=255)
                                                                              cgcgGTGACGGTGGTTAAAAAAGAGAGGGCCCAGGGGCAAAAGCCGAGTCGCTTTATTGCCTGGACCTTTATGACCGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCgg                                                                          <  2:3791117/150‑1 (MQ=255)
                                                                              cgcgGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCgg                                                                          <  2:8982031/150‑1 (MQ=255)
                                                                               gcgGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCggg                                                                         <  1:2385160/150‑1 (MQ=255)
                                                                                     aaGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTcgcg                                                                   <  1:6179993/150‑1 (MQ=255)
                                                                                        gtggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGccc                                                                <  1:344408/150‑1 (MQ=255)
                                                                                            ttAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAg                                                            <  2:5976542/150‑1 (MQ=255)
                                                                                            ttAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAg                                                            <  1:5967448/150‑1 (MQ=255)
                                                                                            ttAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAg                                                            <  2:3322215/150‑1 (MQ=255)
                                                                                            ttAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAg                                                            <  1:523256/150‑1 (MQ=255)
                                                                                             tAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGACCTTTATGAAACACGAGCACCCCCGCCGGTTTGTCCAACGCCAGCGTTAAATCGTTGGTTACCCCCGCGGGAACGGTTCAGCCGGGGTCGCGCCCGGAgc                                                           >  2:4375476/1‑150 (MQ=255)
                                                                                             tAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgc                                                           <  1:404381/150‑1 (MQ=255)
                                                                                              aaaaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgca                                                          <  1:5347991/150‑1 (MQ=255)
                                                                                              aaaaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgca                                                          <  2:1010049/150‑1 (MQ=255)
                                                                                                aaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCAGGACCGTTATGAAAGACGAGCAGCGCCGCCCTTTTGTCAATCGCCCGCGGTAAAGCGTTGGTTCCCCCCGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgcagg                                                        >  2:8174863/1‑150 (MQ=255)
                                                                                                aaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgcagg                                                        <  1:6864961/150‑1 (MQ=255)
                                                                                                aaaaGAGAGGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgcagg                                                        <  2:6285985/150‑1 (MQ=255)
                                                                                                 aaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCGGGACCTTTATGAACGACGCCCAGCGCCGCCGGTTTGTCAATCGCCCGCGTTAAAGCGTTGGTTCCCCCCGCGGTAACCGGTCAGCCCGGGTCGCGCCCGGCgcaggc                                                       >  2:8755688/1‑150 (MQ=255)
                                                                                                          gCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGa                                              <  2:6348175/150‑1 (MQ=255)
                                                                                                            ccAGGGGCAAAAGCAGAGTCGCGTTATTTCCGGGACCTTTATGAACGACGGGCAGCGCCCCCGGTTTGTCCATCGCCCGCGGTAAAGCGGTGGGTCCCCCGGCGGTAACGGGTCAGCCGGGGGCGCGCCCCGGGCAGGCCGCACCGGATa                                            >  2:2597406/1‑150 (MQ=255)
                                                                                                            ccAGGGGCAAAAGCAGAGTCGCGTTATTGCCAGGACCATTATGAACGACCAGCAGCGCCGCCCGTTTGTCCATAGCCAGCGGTAAAGCGGTGGTTCCCCCCGCGGTAACGGGTCAGCCCGGGGCGCGCCCGGAGCAGGCCCCACCAGATa                                            >  2:1067168/1‑150 (MQ=255)
                                                                                                              aGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCCGTATGAACGGCCCGCCGCGCCCCCGGTGTGTCCAACCCCACCGGTAAAGCCGGGGGTCCCCCCGCGGGAACCGGGCAGCCCGGGGCGCCCCCGGAGCCCGCAGCAccagcgaag                                          >  2:7385167/1‑143 (MQ=255)
                                                                                                               ggggCAAAAGCAGCGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGt                                         <  2:2459395/150‑1 (MQ=255)
                                                                                                                  gCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTg                                      <  1:2506996/150‑1 (MQ=255)
                                                                                                                  gCAAAAGCAGAGTCGCTTTAGTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTg                                      <  1:7762659/150‑1 (MQ=255)
                                                                                                                      aaGCAGAGTCGCGTTATTTCCGGGACCTTTATGAACCACGAGCAGCCCCGCCGTTTTGTCAATCGCCAGCGGTAAAGCCTTTGTTCCCCCCGCGGTAAACGTTCAGCCCGGGGCGCGCCCGGAGCAGGCACCACCTGATAAGTCTGCAca                                  >  2:3735698/1‑149 (MQ=255)
                                                                                                                       aGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTg                                 <  1:4826194/150‑1 (MQ=255)
                                                                                                                       aGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTg                                 <  1:637566/150‑1 (MQ=255)
                                                                                                                       aGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTg                                 <  1:7112368/150‑1 (MQ=255)
                                                                                                                         cAGAGTCGCTTTATTTCCTGGACCATTATGAAACACGAGCAGCGCCCCCCTTTTTTCAATACCCAGCGTTAAAGCGTTTGTTACCCCCGCGGTAACCGTTCATCCGGGGTCGCGCCCCGAGCAGGCAGCACCTGATAAGTCTGCACAGgc                               >  2:5159261/1‑150 (MQ=255)
                                                                                                                             gTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGgcgcgc                           <  2:4599146/150‑1 (MQ=255)
                                                                                                                                gCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGaa                        <  2:4885297/150‑1 (MQ=255)
                                                                                                                                  tttATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcg                      >  2:2862269/1‑150 (MQ=255)
                                                                                                                                  tttATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcg                      >  1:6728671/1‑150 (MQ=255)
                                                                                                                                  tttATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcg                      >  1:5773942/1‑150 (MQ=255)
                                                                                                                                    tATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgcc                    <  1:384958/150‑1 (MQ=255)
                                                                                                                                      ttGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgcccg                  >  2:1520559/1‑150 (MQ=255)
                                                                                                                                       tGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgcccgc                 >  2:4030719/1‑150 (MQ=255)
                                                                                                                                         ccTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCa               >  1:8064683/1‑150 (MQ=255)
                                                                                                                                         ccTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCa               >  1:327187/1‑150 (MQ=255)
                                                                                                                                             ggACCTTTATTAACGACGAGCAGCGCCGCGTTTTTGTAAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCGGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGt            <  1:7925846/150‑1 (MQ=255)
                                                                                                                                             ggACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGt            >  1:5675187/1‑150 (MQ=255)
                                                                                                                                              gACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTc           <  2:6649992/150‑1 (MQ=255)
                                                                                                                                              gACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTc           <  2:5437128/150‑1 (MQ=255)
                                                                                                                                               aCCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGTTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTTATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCg          <  1:1036809/150‑1 (MQ=255)
                                                                                                                                                  tttATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGaaa       >  1:119939/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
CAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTT  >  NC_000913/843067‑843365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: