Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,227 A→C 6.5% intergenic (‑188/+180) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2270AC 6.5% 1133.7 / 5.6 386intergenic (‑188/+180)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base A (138/208);  new base C (34/2);  total (173/213)
Fisher's exact test for biased strand distribution p-value = 5.40e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 400 total aligned reads displayed.

AATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAG  >  NC_000913/1351078‑1351376
                                                                                                                                                      |                                                                                                                                                     
aaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                                                       <  1:1215279/150‑1 (MQ=255)
  tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                     <  1:8367822/150‑1 (MQ=255)
      gCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCACCACACCCAAAACCCCACCCCACAgggg                                                                                                                                                 >  2:2013213/1‑150 (MQ=255)
          ttCGTCGTTTTAGGAAACAGTACGAACAGATCAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTGTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAg                                                                                                                                             <  1:6605905/150‑1 (MQ=255)
           tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGTGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                            <  1:3688603/150‑1 (MQ=255)
           tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                            <  2:6584297/150‑1 (MQ=255)
           tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                            <  2:4358286/150‑1 (MQ=255)
           tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                            <  2:4129027/150‑1 (MQ=255)
              tcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                                                                         <  1:6942177/150‑1 (MQ=255)
                gtTTTAGTAAACAGTACGAACAGATCAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATCGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCGCCCCTCTGGGGAGAgggttag                                                                                                                                       <  2:3853306/150‑5 (MQ=255)
                 ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCg                                                                                                                                      >  2:9130356/1‑150 (MQ=255)
                  tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                                                                                     >  2:1123735/1‑150 (MQ=255)
                     aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                  <  2:3596212/150‑1 (MQ=255)
                     aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                  <  2:4280867/150‑1 (MQ=255)
                       tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                >  1:3871749/1‑150 (MQ=255)
                       tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCGCACCCAAACCCACTCCCCCGAGGGGCGAGGGGACCGTATtgt                                                                                                                                >  1:731675/1‑150 (MQ=255)
                         aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCGGATAGTGc                                                                                                                              >  2:8903031/1‑150 (MQ=255)
                         aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCGCACCCCCGAGGGGCGCGGGGGCCGGAGtgtgg                                                                                                                              >  1:1928711/1‑149 (MQ=255)
                            aGTACGAACAGATAACCGGTTATTATACTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                                                                           <  1:5885138/150‑1 (MQ=255)
                            aGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                                                                           <  1:1368177/150‑1 (MQ=255)
                                cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                       <  2:565334/150‑1 (MQ=255)
                                 gAACAGATAAACGGTTATTATCATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTg                                                                                                                      <  1:2663668/150‑1 (MQ=255)
                                 gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTg                                                                                                                      >  2:5522293/1‑150 (MQ=255)
                                   aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACCACCAAACCCACCCCCCCCAGGGGCGAGGGGACCCAAGTGTGGaaaaaatttt                                                                                                                    >  1:8608806/1‑143 (MQ=255)
                                    cataTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTa                                                                                                                   <  1:3216944/147‑1 (MQ=255)
                                    cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCTCACCCAAACACACAACCCCCAGGGGCGAGGGGACCCGAATGTGCAAATATAGTTa                                                                                                                   >  1:5772730/1‑150 (MQ=255)
                                     aGATAACCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                                                                                  <  2:4828013/150‑1 (MQ=255)
                                        tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAcccc                                                                                                               <  2:797396/150‑1 (MQ=255)
                                          aaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCAAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAg                                                                                                             <  1:3138919/150‑1 (MQ=255)
                                               ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACAACCAAACCCGCTCCCCCGAGGGGCGAGGGGACCGGATTGTGCAAATATTATTACCCCAGccaca                                                                                                        >  2:3714528/1‑150 (MQ=255)
                                                 attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCCCACCCAAAACCCCACCCCCGAGGGGCGCGGGGGCCGGATTGTGCCAAAATTGTTTACCCCCCaacaaa                                                                                                      >  2:8544693/1‑150 (MQ=255)
                                                   tataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaaca                                                                                                    <  1:7971249/150‑1 (MQ=255)
                                                   tataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCATATTGTGCAAATATTGTTACCCCAGCaacaaaca                                                                                                    <  2:7851934/150‑1 (MQ=255)
                                                   tataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACTCCCCAGAGGGGCGAGGGGACCGGAGTGTGCAAATATTGTTACCCCAGCaacaaaca                                                                                                    >  2:5920892/1‑150 (MQ=255)
                                                     taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                  <  1:8812816/150‑1 (MQ=255)
                                                     taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                  <  1:1059825/150‑1 (MQ=255)
                                                      aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCGTAATGTGCAAATATTGTTACCCCACCAAAAAAAAGGc                                                                                                 >  2:2915009/1‑150 (MQ=39)
                                                        tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCGCACCCTAACCCAAACCCCCGGGGGGCGAGGGGACCCGGATGTGCAAATAATGTTAACCCAACCACAAAAAGGCTc                                                                                               >  1:5318933/1‑150 (MQ=255)
                                                        tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGAAGGCTGCGGCAGGGGGGGCGCTTTTGCCCACCACCAAAACCAATCCCCCCAGGGGCGCGGGGACCCTATTTTGCGAAAAATTTTAACCCAACaacaaaaacgcga                                                                                               >  2:1577256/1‑143 (MQ=255)
                                                           aCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCAACCTAAACCTCTCCACAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACACCAGCAACAACCAGGAACATa                                                                                            >  2:1302779/1‑150 (MQ=255)
                                                            ccTGGCTGAGAGTAGCTATAGTTGCCAGGTCCCACCGGAGCAGGCTGCGGCAGGGGGGGCGCAATTCCCCTCACCCTAACCCACTCCCCACAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCACCAACAAACAGGCACATAc                                                                                           <  2:4389759/150‑1 (MQ=255)
                                                               ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                        <  1:7196541/150‑1 (MQ=255)
                                                                 cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGccc                                                                                      <  1:3520098/150‑1 (MQ=255)
                                                                   gtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCGCACCCCAAACCACACCCCACAGGGGCGAGGGGGCCCGAGTGTGCAAAAATTTTTACCCCCACAACAAAAACGCGCATACACccccc                                                                                    >  1:6169973/1‑149 (MQ=255)
                                                                     gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                  <  1:4710930/150‑1 (MQ=255)
                                                                     gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                  <  1:6305117/150‑1 (MQ=255)
                                                                     gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                  <  1:6764856/150‑1 (MQ=255)
                                                                       ggaGCGATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                <  2:9019071/148‑1 (MQ=255)
                                                                       gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                <  2:640923/150‑1 (MQ=255)
                                                                       gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                <  2:7515055/150‑1 (MQ=255)
                                                                       gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACTCCCCAGAGGGGCCTGGGGACCGGATTGTGCAAATAATGTTACCCCCGCAACAAACAGGCACATACAGCCCCAAAcc                                                                                >  2:2330711/1‑150 (MQ=255)
                                                                       gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCGAAACCACACCCCCCAGGGGGGCGGGGGCCCGATTTTTCAAAAATTTTTTACCCACCAAAAAAAAAGCGCAAAAAGCCCCAAAcc                                                                                >  1:8000354/1‑150 (MQ=255)
                                                                          gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCACCCCCCAACCCAACACCCCCACGGGCGGGGGGGCCCGTGTGGGGAAAAAATTTTTAACCCACAACAAAACCGCACATACAACCCCAAAcccca                                                                             >  1:6523427/1‑148 (MQ=255)
                                                                          gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCACACCCCACAGGGGCGAGGGGGCCGGATTGTGCAAATATTTTTAACCCAGCAACCAACAGGGGCATAAAGCCCCAAACCCtt                                                                             >  1:7698366/1‑150 (MQ=255)
                                                                            tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCGAACCCGCACCCCAGAGGGGCGAGGGGCCCCGATTGTGCAAATATTGTTACCCCACCAACAAACAGGCGCATACAGCCCCGAACCCATTc                                                                           >  2:862808/1‑150 (MQ=255)
                                                                            tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAACCCACACCCCAGATGGGCGGGGGGGCCCGATTTTGCAAAAATTTTTTCCCCCCCAAAAAACACGCGCAAACACCCCCCAAACCattg                                                                           >  1:7617692/1‑149 (MQ=255)
                                                                             ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCTCCCCAGAGGGGCGAGGGGACCGTAATGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                                                                          >  2:8432546/1‑150 (MQ=255)
                                                                              taGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCAt                                                                         >  2:5402064/1‑150 (MQ=255)
                                                                               aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATg                                                                        <  2:25075/150‑1 (MQ=255)
                                                                                gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                       <  1:4240215/150‑1 (MQ=255)
                                                                                gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                       <  1:2082633/150‑1 (MQ=255)
                                                                                 ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc                                                                      <  2:7643619/150‑1 (MQ=255)
                                                                                 ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCGCACCCTAAACCGCTCCCCAGAGGGGCGCGGGGGCCCTATTGTTCAAAAATTTTTACCCCAGCCACAAACAGGCGCATACAGCCCCCAACCCATTCAAGGc                                                                      >  2:3427281/1‑150 (MQ=255)
                                                                                   gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGa                                                                    <  1:7505331/150‑1 (MQ=255)
                                                                                    ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAAACCACACCCCCGAGGGGCGCGGGGCCCGGATTGTGCAAAAATTGTTTCCCCCCCAACAAAAAGGCGCAGACAGCCCCGAAACCATTAATGGCGag                                                                   >  2:1749193/1‑149 (MQ=255)
                                                                                    aaaGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCACTCCCCAGAGGGGCGAGGGGACCCTATTGTGCAAATAAAGTTACACCAGCAACAAACAGGCTAATACAGCCCCTAACCCATTCAATGCGAt                                                                   >  2:2128136/3‑150 (MQ=37)
                                                                                        gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCt                                                               <  2:6854922/150‑1 (MQ=255)
                                                                                           cGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTGCCCCAGCAACAAACAGGCTCAAACAGCCCCGAACCCTTTCATGGCGATGGCTggg                                                            >  2:8371396/1‑150 (MQ=255)
                                                                                             aCCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGACCCTAACCCTTTCATGGCGATGGCTGGGAc                                                          >  2:4921522/1‑150 (MQ=255)
                                                                                             aCCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAAACCACACCCCACAGGGGCGAGGGGGCCCTAATGTTCAAAAATTTTTAACCCAGCAAAAAACAGGCTCAAACAGCCCCAAACCCATTAAAGGCGATGGCTggggc                                                          >  2:66528/1‑148 (MQ=255)
                                                                                              ccGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACg                                                         <  2:6190294/150‑1 (MQ=255)
                                                                                                ggAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGt                                                       <  1:5198765/150‑1 (MQ=255)
                                                                                                 gAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                      <  1:1868976/150‑1 (MQ=255)
                                                                                                   gCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCa                                                    <  1:2584182/150‑1 (MQ=255)
                                                                                                    taGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCTAACCCACTCCCCAGAGGGGCGAGGGGACCCGATTGTGCAAATATTTTTAACCCAGCAACAAACAGGCTCAAACAGCCCCAAACCCTTTCATGGCGATGGCTGGGCCGGTTCAg                                                   >  2:7295330/2‑150 (MQ=38)
                                                                                                            ggCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGcc                                           <  2:1874996/150‑1 (MQ=255)
                                                                                                                 ggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAata                                      <  2:4579212/150‑1 (MQ=255)
                                                                                                                      gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                 <  2:6115357/150‑1 (MQ=255)
                                                                                                                         cTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAg                              <  1:8971431/150‑1 (MQ=255)
                                                                                                                                    aCCCTAACCCTCTCCCCAGAGGGGCGAGTGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACATTGCCGAATATTCTCCAGCACCGTCTCCa                   <  2:5489772/150‑1 (MQ=255)
                                                                                                                                     cccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCAt                  <  2:8148037/150‑1 (MQ=255)
                                                                                                                                       cTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGt                >  1:4366525/1‑150 (MQ=255)
                                                                                                                                       cTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGt                >  1:4105818/1‑150 (MQ=255)
                                                                                                                                          aCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtttt             <  1:5493341/150‑1 (MQ=255)
                                                                                                                                            ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca           <  1:1616541/150‑1 (MQ=255)
                                                                                                                                            ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca           >  1:4605819/1‑150 (MQ=255)
                                                                                                                                             ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac          <  2:3183414/150‑1 (MQ=255)
                                                                                                                                             ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac          >  1:8244780/1‑150 (MQ=255)
                                                                                                                                               tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAGTATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc         >  2:6455509/1‑150 (MQ=255)
                                                                                                                                               tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc         >  1:5378150/1‑150 (MQ=255)
                                                                                                                                               tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc         >  1:2354749/1‑150 (MQ=255)
                                                                                                                                                ctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacca        <  2:5279671/150‑1 (MQ=255)
                                                                                                                                                 tcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcaccac       >  1:3321300/1‑150 (MQ=255)
                                                                                                                                                   cccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaa     >  2:4095907/1‑150 (MQ=255)
                                                                                                                                                    ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGATGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac    <  1:9130356/150‑1 (MQ=255)
                                                                                                                                                    ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac    >  2:472304/1‑150 (MQ=255)
                                                                                                                                                     cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca   >  2:8270506/1‑150 (MQ=255)
                                                                                                                                                     cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca   >  2:2781979/1‑150 (MQ=255)
                                                                                                                                                      agagGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAg  <  2:7120875/150‑1 (MQ=255)
                                                                                                                                                      agagGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAg  <  1:2911023/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAG  >  NC_000913/1351078‑1351376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: