Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,630,528 T→G 10.7% Y129S (TAT→TCT)  ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,630,5280TG10.7% 1430.7 / 135.2 452Y129S (TAT→TCT) ydfIputative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (192/211);  new base G (26/23);  total (218/234)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 458 total aligned reads displayed.

CGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACATTTCC  >  NC_000913/1630379‑1630677
                                                                                                                                                     |                                                                                                                                                     
    aaaTGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGAccc                                                                                                                                                   <  2:7327016/150‑1 (MQ=255)
         cGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCttttt                                                                                                                                              >  2:1514342/1‑150 (MQ=255)
          gggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTc                                                                                                                                             >  1:9096021/1‑150 (MQ=255)
           ggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCg                                                                                                                                            <  2:3066441/150‑1 (MQ=255)
               gTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGa                                                                                                                                        <  2:6886051/150‑1 (MQ=255)
                tCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGAt                                                                                                                                       >  2:6975258/1‑150 (MQ=255)
                 ccTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGAtt                                                                                                                                      <  1:124367/150‑1 (MQ=255)
                   tGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGt                                                                                                                                    >  1:6852823/1‑150 (MQ=255)
                   tGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGt                                                                                                                                    >  2:6437247/1‑150 (MQ=255)
                    gCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTc                                                                                                                                   >  2:5023815/1‑150 (MQ=255)
                     ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGCAATACCCTTTGTCGGTGATTGTCa                                                                                                                                  >  1:7234527/1‑150 (MQ=255)
                     ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCa                                                                                                                                  >  1:2623236/1‑150 (MQ=255)
                     ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCa                                                                                                                                  <  1:5039546/150‑1 (MQ=255)
                     ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTCGGTGATTGTCag                                                                                                                                 >  2:1140276/1‑150 (MQ=255)
                       gCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGTGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCaga                                                                                                                                <  2:8368705/150‑1 (MQ=255)
                       gCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCaga                                                                                                                                <  2:3965162/150‑1 (MQ=255)
                       gCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCaga                                                                                                                                >  2:2031356/1‑150 (MQ=255)
                         tttACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCagaga                                                                                                                              <  1:3507499/150‑1 (MQ=255)
                          ttACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCagagag                                                                                                                             >  2:7385570/1‑150 (MQ=255)
                                cGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATc                                                                                                                       <  2:5926737/150‑1 (MQ=255)
                                 gAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcg                                                                                                                      <  1:4291669/150‑1 (MQ=255)
                                 gAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcg                                                                                                                      <  2:4539592/150‑1 (MQ=255)
                                 gAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcg                                                                                                                      >  1:7952178/1‑150 (MQ=255)
                                  aGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcgc                                                                                                                     <  1:5195347/150‑1 (MQ=255)
                                    ccAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGa                                                                                                                   >  1:7550979/1‑150 (MQ=255)
                                      aGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAttt                                                                                                                >  2:5544494/1‑150 (MQ=255)
                                        cgcCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTg                                                                                                               <  1:3109804/150‑1 (MQ=255)
                                          ccTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCg                                                                                                             <  2:5476937/150‑1 (MQ=255)
                                          ccTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCg                                                                                                             <  1:7798643/150‑1 (MQ=255)
                                           cTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCTGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCgg                                                                                                            >  2:935988/1‑150 (MQ=255)
                                             caacaaTCCCCCCGGTCGCCGTATTCGGCCGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGGCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtt                                                                                                          <  1:6017981/150‑1 (MQ=255)
                                             caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtt                                                                                                          <  2:298767/150‑1 (MQ=255)
                                             caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtt                                                                                                          >  1:1806087/1‑150 (MQ=255)
                                             caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtt                                                                                                          >  1:6055130/1‑150 (MQ=255)
                                              aacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCCTCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtgc                                                                                                         >  1:1393598/1‑148 (MQ=255)
                                                    cACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACAt                                                                                                   <  1:6471243/150‑1 (MQ=255)
                                                     aCCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATc                                                                                                  >  2:1378233/1‑150 (MQ=255)
                                                      ccccTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCg                                                                                                 <  2:2670402/150‑1 (MQ=255)
                                                             tGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCaa                                                                                          >  1:8756218/1‑150 (MQ=255)
                                                                tGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAacac                                                                                       <  1:6659259/150‑1 (MQ=255)
                                                                tGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAacac                                                                                       >  1:5093680/1‑150 (MQ=255)
                                                                tGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAacac                                                                                       >  2:8948894/1‑150 (MQ=255)
                                                                 gTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACg                                                                                      >  1:5955246/1‑150 (MQ=255)
                                                                 gTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACg                                                                                      <  2:1322876/150‑1 (MQ=255)
                                                                     tCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGttt                                                                                  <  1:185885/150‑1 (MQ=255)
                                                                      cGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTc                                                                                 <  2:5252834/150‑1 (MQ=255)
                                                                      cGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTc                                                                                 <  2:3902493/150‑1 (MQ=255)
                                                                         cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTaa                                                                              <  2:3790556/150‑1 (MQ=255)
                                                                         cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTaa                                                                              <  2:8806253/150‑1 (MQ=255)
                                                                          tggtggGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAg                                                                             >  1:2989465/1‑150 (MQ=255)
                                                                          tggtggGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAAACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAg                                                                             <  1:2102331/150‑1 (MQ=255)
                                                                            gtggGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGcc                                                                           <  1:5403626/150‑1 (MQ=255)
                                                                            gtggGGATTTTGCACGTCGACAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGcc                                                                           >  1:6280020/1‑150 (MQ=255)
                                                                             tggGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCa                                                                          <  2:9032732/150‑1 (MQ=255)
                                                                               gggATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGTGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatc                                                                        >  2:1054302/1‑150 (MQ=255)
                                                                                ggATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatct                                                                       >  2:6607702/1‑150 (MQ=255)
                                                                                   ttttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatctatc                                                                    <  2:7653767/150‑1 (MQ=255)
                                                                                    tttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatctatct                                                                   <  2:3517322/150‑1 (MQ=255)
                                                                                    tttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatctatct                                                                   <  2:6521752/150‑1 (MQ=255)
                                                                                         aCGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACg                                                              >  1:4818057/1‑150 (MQ=255)
                                                                                         aCGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACg                                                              >  1:8232573/1‑150 (MQ=255)
                                                                                              ggCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACATTCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTAAGTGaaa                                                         <  2:815734/150‑1 (MQ=255)
                                                                                              ggCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCaa                                                         <  1:890960/150‑1 (MQ=255)
                                                                                                     aCCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCtttt                                                  >  1:2413269/1‑150 (MQ=255)
                                                                                                      ccATCGGGTGATCGAGCATTAACTGTCTGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCttttt                                                 <  1:7376124/150‑1 (MQ=255)
                                                                                                       cATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCACGGCtttttt                                                >  1:1687722/1‑150 (MQ=255)
                                                                                                       cATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCtttttt                                                >  2:5873830/1‑150 (MQ=255)
                                                                                                        aTCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTa                                               >  1:1054700/1‑150 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              >  2:2153700/1‑150 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              <  2:6048579/150‑1 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              <  2:5593527/150‑1 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              <  2:1780450/150‑1 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTGTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              <  2:5728221/150‑1 (MQ=255)
                                                                                                          cGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAac                                             <  2:2519306/150‑1 (MQ=255)
                                                                                                           gggTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAacg                                            <  1:5681611/150‑1 (MQ=255)
                                                                                                           gggTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCACTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAacg                                            >  1:3919525/1‑150 (MQ=255)
                                                                                                            ggTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAacga                                           >  2:7973842/1‑150 (MQ=255)
                                                                                                             gTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAacgac                                          >  1:370968/1‑150 (MQ=255)
                                                                                                             gTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAacgac                                          <  2:6268697/150‑1 (MQ=255)
                                                                                                                 tCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCa                                      >  2:2457152/1‑150 (MQ=255)
                                                                                                                   gAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAac                                    >  2:7164221/1‑150 (MQ=255)
                                                                                                                   gAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAac                                    >  1:5607794/1‑150 (MQ=255)
                                                                                                                        ttAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGAcgc                               >  2:4263420/1‑150 (MQ=255)
                                                                                                                         tAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGAcgcg                              <  2:2784988/150‑1 (MQ=255)
                                                                                                                             tGTCCGGTCGCGGTAGAGTGTAAAGACCCTTTTTGGGTGTTTGTCTGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCAAACACGGTTTCTATGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGcc                          <  1:8498160/150‑1 (MQ=255)
                                                                                                                              gTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCg                         >  2:4747930/1‑150 (MQ=255)
                                                                                                                               tCCGGTCGCCGGAGAGTGGACATACCCTTGGTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACCCATCGCTGCCACCACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGt                        <  2:2367407/150‑1 (MQ=255)
                                                                                                                               tCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCTGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGTTTTCTAAGCCATCTATCTGTACGTGCATGGATTTTTTAACGACGCCAACGACGCGAGCCGt                        <  1:3792686/150‑1 (MQ=255)
                                                                                                                               tCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGt                        >  1:4207068/1‑150 (MQ=255)
                                                                                                                               tCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGt                        >  1:8395047/1‑150 (MQ=255)
                                                                                                                                  ggTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCca                     <  2:338846/150‑1 (MQ=255)
                                                                                                                                    tCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCcaca                   >  2:1679328/1‑150 (MQ=255)
                                                                                                                                        cGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTACCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATc               >  1:3217215/1‑150 (MQ=255)
                                                                                                                                              gTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGa         >  1:8328298/1‑150 (MQ=255)
                                                                                                                                               tGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGAc        >  2:4335350/1‑150 (MQ=255)
                                                                                                                                               tGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGAc        >  2:8297592/1‑150 (MQ=255)
                                                                                                                                                ggAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACa       >  2:4110177/1‑150 (MQ=255)
                                                                                                                                                 gAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACAt      >  1:6700548/1‑150 (MQ=255)
                                                                                                                                                    aTACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACATTTc   <  2:8715127/150‑1 (MQ=255)
                                                                                                                                                     tACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACATTTcc  <  2:637088/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACATTTCC  >  NC_000913/1630379‑1630677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: