Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,768 T→C 6.8% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7680TC 6.8% 951.4 / 28.4 322intergenic (+34/+14)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (159/126);  new base C (10/10);  total (173/149)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

ATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGA  >  NC_000913/1864619‑1864917
                                                                                                                                                     |                                                                                                                                                       
 tgatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATc                                                                                                                                                        <  2:6370421/150‑1 (MQ=255)
   atgGCTACCAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCg                                                                                                                                                      <  2:4088929/150‑1 (MQ=255)
   atgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCg                                                                                                                                                      <  2:642645/150‑1 (MQ=255)
    tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACTCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgt                                                                                                                                                     <  2:2094427/150‑2 (MQ=255)
    tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                     <  2:5771826/150‑1 (MQ=255)
    tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                     <  1:6789885/150‑1 (MQ=255)
    tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                     <  1:2908988/150‑1 (MQ=255)
    tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                     <  1:1853937/150‑1 (MQ=255)
      gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGG‑‑ca                                                                                                                                                 <  2:5013066/150‑1 (MQ=255)
        tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑caca                                                                                                                                               >  2:8388676/1‑150 (MQ=255)
                  ttgtttgtGTAGAACCGGCTTACGCTTCATAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATcaca                                                                                                                                     <  2:2789629/150‑1 (MQ=255)
                     tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCTTTCGCGTTGCGAAACGTTAATTTAGGTTAATGTTGTGTGCGGGGTGCATTGCATCCGG‑‑CACACAACATCACACCa                                                                                                                                  <  2:8569783/150‑1 (MQ=255)
                     tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAAGCATCCGG‑‑CCCACAACATCACACCa                                                                                                                                  >  2:4714845/1‑150 (MQ=255)
                      ttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCat                                                                                                                                 >  1:2309493/1‑150 (MQ=255)
                           tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATCTCCa                                                                                                                            >  2:8278655/1‑150 (MQ=255)
                           tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCa                                                                                                                            >  1:2141514/1‑150 (MQ=255)
                              aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CCCACAACATCACACCATATCCcgcg                                                                                                                         >  2:2381819/1‑150 (MQ=255)
                              aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAgcg                                                                                                                         >  1:8087457/1‑150 (MQ=255)
                                   gCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGtt                                                                                                                    >  2:2505740/1‑150 (MQ=255)
                                     ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCCTATCCAGCGCAGtttt                                                                                                                  >  2:390921/1‑150 (MQ=255)
                                     ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGtttt                                                                                                                  >  2:9133834/1‑150 (MQ=255)
                                     ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGtttt                                                                                                                  <  2:6111410/150‑1 (MQ=255)
                                         gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTccg                                                                                                              >  1:7479395/1‑149 (MQ=255)
                                          cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCCTCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                             >  1:514035/1‑150 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCCGCGCAGTTTTTccggt                                                                                                            >  1:6646436/1‑147 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                            >  1:1741484/1‑150 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCAAATCCAGCGCAGTTTTTCCttt                                                                                                            >  1:4652253/1‑150 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCAAATCCAGCGCAGTTTTTCCttt                                                                                                            >  1:2041758/1‑150 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAAGCATCAGG‑‑CACACAACATCACACCAAAACCAGCGCAGTTTTTACttg                                                                                                            >  2:3839866/1‑149 (MQ=255)
                                            tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCtgtt                                                                                                           >  2:6282721/1‑150 (MQ=255)
                                             cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                          >  1:4468672/1‑150 (MQ=255)
                                                aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                                       >  1:9154679/1‑150 (MQ=255)
                                                   cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCAGTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                                    <  2:5232618/150‑1 (MQ=255)
                                                     cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                                  >  2:662376/1‑150 (MQ=255)
                                                         aaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATg                                                                                              >  2:3296697/1‑150 (MQ=255)
                                                          aGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                             <  2:7380327/150‑1 (MQ=255)
                                                          aGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                             <  2:926599/150‑1 (MQ=255)
                                                            tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                           >  1:7650333/1‑150 (MQ=255)
                                                            tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                           >  2:3623523/1‑150 (MQ=255)
                                                            tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCCGGTGCAATGCATCCCG‑‑CACACAACATCACACCAAATCCAGCGCCGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                           >  1:8484505/1‑150 (MQ=255)
                                                             gcccAAAGAGAAACCTGCACAGCGGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCtt                                                                                          <  2:4268181/148‑1 (MQ=255)
                                                              aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                                         >  2:5317442/1‑150 (MQ=255)
                                                               ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAAGCATCCGG‑‑CACACAACATAACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGCTATGCCTTAt                                                                                        >  1:8894213/1‑150 (MQ=255)
                                                                cAAATACAACCCTGCACATCTGGCCCAACCCACTCGCGTTGCTAACCTTTATTTTACTTTATTGTTGTGTGCCGGGTCCAATGCACCCGC‑‑AACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                       <  1:7320090/150‑1 (MQ=255)
                                                                  cccagaACCCGGCACCTCTGGCGCAACCCTTGCGCGTTGCTAAACGTTAATTTACTTTATTGTTGTGGGCGGGGTGAAATCCACCCGC‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                                     <  1:2589334/147‑1 (MQ=255)
                                                                   acacaaaCCTCCACAACACGCGCAAACCATACGCGTTGCGAAACGAAAAATAACCATAATCTTGTGTGCCGGGTGCAATCCAACCGC‑‑CACACAACATCACACCAAATCCAGCGCAGTTTTTCCTATTGGTGCCGGATATGCCTTATCCAg                                                                                    <  2:8952648/146‑1 (MQ=255)
                                                                    gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCGGTTGGTGCCGGATATGCCTTATCCAGc                                                                                   >  2:5600211/1‑150 (MQ=255)
                                                                     agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATCCGG‑‑CACACACCAACACACCAAATCCAGCGCAGTTTTTCCTGTTGGTGCCGGCTATGCCTTATCCAGCa                                                                                  >  1:4109270/1‑150 (MQ=255)
                                                                           cTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                     <  2:7521796/79‑1 (MQ=255)
                                                                           cTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTa                                                                            >  2:566509/1‑150 (MQ=255)
                                                                            tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGAATACCG‑‑CAAACAACAAAACACCAAATCCACCGCAGTTTTTACTTTTGGTGCCGGATATGCCAGATCCAGCCTAGCTaa                                                                           >  1:5398219/1‑150 (MQ=255)
                                                                            tGCACATCGGGCGAAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGAAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTaa                                                                           <  1:8296092/150‑1 (MQ=255)
                                                                             gCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAt                                                                          >  2:3043822/1‑150 (MQ=255)
                                                                             gCACATCTGGCGAAATCCATTCGCGTTGCGAAACTTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAt                                                                          <  1:531067/150‑1 (MQ=255)
                                                                              cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAtg                                                                         >  2:1375984/1‑149 (MQ=255)
                                                                               acaTCGGGCGAAATCCATTCGCGTTGCTAAACGTTAATTTACTTTATTGTTGTGGGCGGGGTGCAATGCAGCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTc                                                                        <  1:4207808/150‑1 (MQ=255)
                                                                                  tCTGGCGCAATCCTTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                                     <  2:1572247/150‑1 (MQ=255)
                                                                                  tCTGCCCCAATCCATACGCGTTCCCAAACCAAAATTAACGTTAATGTTGTGTCCCGGGTCCAATCCAACCGC‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                                     <  1:698112/150‑1 (MQ=255)
                                                                                   cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                    <  1:1356504/150‑1 (MQ=255)
                                                                                   cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                    <  1:6008031/150‑1 (MQ=255)
                                                                                     ggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTga                                                                  <  1:3539522/150‑1 (MQ=255)
                                                                                                ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGGGCAAATCATCCGG‑‑CACAAAACAACACACCAAAAACACCGCACGTTTTACGGTTGGTGCCGGATATTCCGTATCCAGCCTAGATAAATACGCGGAAGAAAGTTCGa                                                       >  1:932436/1‑150 (MQ=255)
                                                                                                ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                       >  2:7420751/1‑150 (MQ=255)
                                                                                                ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCCGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGGAGAAAGTTCGa                                                       >  2:4803493/1‑150 (MQ=255)
                                                                                                 tCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAc                                                      <  2:8103475/150‑1 (MQ=255)
                                                                                                     gTTGCGAAACGTTAATTTACGTTAATGTTGTGGGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                                  <  1:1439765/150‑1 (MQ=255)
                                                                                                       tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                                <  1:4918012/150‑1 (MQ=255)
                                                                                                         cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                              >  2:3347019/1‑150 (MQ=255)
                                                                                                         cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                              <  2:1707395/150‑1 (MQ=255)
                                                                                                           aaaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACATAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                            >  1:6135420/1‑150 (MQ=255)
                                                                                                            aaCGTTAATTTACGTTAATGTTGTGTGCCCGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCGAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAg                                           >  2:3187694/1‑150 (MQ=255)
                                                                                                             aCGTTAATTTACGTTATTGTTTTGTGCCGGGTGAAAGGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGc                                          <  1:7744740/150‑1 (MQ=255)
                                                                                                             aCGTTAATTTACGTTAATGTTGAGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGc                                          <  1:8375648/150‑1 (MQ=255)
                                                                                                               gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCg                                        >  2:2903468/1‑150 (MQ=255)
                                                                                                                       aCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtttt                                  <  2:6945502/150‑1 (MQ=255)
                                                                                                                        ctttAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                               <  1:3285405/148‑1 (MQ=255)
                                                                                                                        cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                               <  2:5996502/150‑1 (MQ=255)
                                                                                                                           tAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGa                            <  1:7163801/150‑1 (MQ=255)
                                                                                                                           tAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGa                            <  2:4518229/150‑1 (MQ=255)
                                                                                                                            aaTGTTGTGTGCCGGGTGCAATGCATACGG‑‑CACACAACATCACACCAAAACCAGCGCAGTTTTTACTTTTGGTGCCGGATATGCCGTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAATTTGTTTGAc                           >  2:7735794/1‑150 (MQ=255)
                                                                                                                                ttgtGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCCGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCACTGAAGAAAGTTCGACCTCAAGCACAGCCGCCTTTTGTTGGagctgg                       >  2:1841274/1‑145 (MQ=255)
                                                                                                                                ttgtGTGCCGGGTGCAATGCACCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                       <  1:8576496/150‑1 (MQ=255)
                                                                                                                                  gtgtgCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGc                     >  2:7949167/1‑150 (MQ=255)
                                                                                                                                   tgtgCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa                    >  1:3808471/1‑150 (MQ=255)
                                                                                                                                     tgCCGGATGCATTGCACCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt                  >  1:3634568/1‑150 (MQ=255)
                                                                                                                                       ccGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg                >  1:7232549/1‑150 (MQ=255)
                                                                                                                                       ccGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAACCGCATTTTGTTGGACATGGGCAATCg                >  1:4375399/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCATTGCACCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg                >  1:2634933/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCATTGCACCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg                >  1:1636818/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCATTGCACCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg                >  1:5549179/1‑150 (MQ=255)
                                                                                                                                         gggTGCAATGCAACCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg              <  1:6541525/150‑1 (MQ=255)
                                                                                                                                         gggTGAAATGCAACCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg              <  1:23247/150‑1 (MQ=255)
                                                                                                                                          ggTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg             <  2:944038/150‑1 (MQ=255)
                                                                                                                                           gTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc            <  1:8747349/150‑1 (MQ=255)
                                                                                                                                            tGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGcc           >  2:1131617/1‑150 (MQ=255)
                                                                                                                                                   cATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg    <  1:7851759/150‑1 (MQ=255)
                                                                                                                                                   cATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg    >  2:4357310/1‑150 (MQ=255)
                                                                                                                                                   cATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg    <  1:1164246/150‑1 (MQ=255)
                                                                                                                                                     tCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGa  >  2:2805065/1‑150 (MQ=255)
                                                                                                                                                     tCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTATTGGTGCCGGATATGCCTTATCCAGCAAAGCTAATTCCGCTGAAGAAAGTTCTACCTCAAGCACATCAGCATATTGTTGGACATGGGCAATCGTGGCCGCTTTTGGa  >  2:6746472/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
ATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGG‑‑CACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGA  >  NC_000913/1864619‑1864917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: