Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,427 C→A 15.9% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4270CA15.9% 1093.6 / 53.8 409Q433K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (109/219);  new base A (76/3);  total (187/222)
Fisher's exact test for biased strand distribution p-value = 5.75e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 419 total aligned reads displayed.

TGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCC  >  NC_000913/2470279‑2470576
                                                                                                                                                    |                                                                                                                                                     
tGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCa                                                                                                                                                      <  2:5201196/150‑1 (MQ=255)
 gtagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaa                                                                                                                                                     <  1:5483894/150‑1 (MQ=255)
  tagtaTCTCTTTTTGTAATATCTCAATTTTATGTGTCATTTTTCTGGGAAGGATATATAGATTTAAGAAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaaa                                                                                                                                                    <  2:2871407/150‑1 (MQ=255)
   agtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaaat                                                                                                                                                   >  1:6764137/1‑149 (MQ=255)
     taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGaa                                                                                                                                                 >  2:6198443/1‑150 (MQ=255)
       tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAtaaaataaataaaataaaa                                                                                                                                               >  2:2450047/1‑149 (MQ=255)
        ctctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAtaaaataaataaaataaaaa                                                                                                                                              >  2:2684159/1‑148 (MQ=255)
         tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATaaaataaataaaataaataa                                                                                                                                             >  2:8681804/1‑148 (MQ=255)
         tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAtaa                                                                                                                                             >  1:488741/1‑148 (MQ=255)
         tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAATTAaaataaataa                                                                                                                                             >  2:4855223/1‑148 (MQ=255)
          ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                            <  1:351885/150‑1 (MQ=255)
          ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                            <  2:5612675/150‑1 (MQ=255)
            ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                          <  1:7827734/150‑1 (MQ=255)
                ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGACAAt                                                                                                                                      >  1:4636437/1‑150 (MQ=255)
                 gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                     <  1:1902470/150‑1 (MQ=255)
                 gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                     <  2:9071342/150‑1 (MQ=255)
                   atatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                                                                                                   <  1:6922675/150‑1 (MQ=255)
                    tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                  <  2:9129173/150‑1 (MQ=255)
                     atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGAATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                 <  2:3175572/150‑1 (MQ=255)
                      tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                <  2:1223555/150‑1 (MQ=255)
                       ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGACAATAGCGATg                                                                                                                               >  1:2976149/1‑150 (MQ=255)
                        tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGACAAAAGAGATGt                                                                                                                              >  2:3038384/1‑150 (MQ=255)
                        tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATAAAGAAAAAAGCGATGt                                                                                                                              >  2:5920639/1‑150 (MQ=255)
                            ttttATGTGTCATTTTTCGGGGAAGTATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                          <  2:7730318/150‑1 (MQ=255)
                            ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                          <  1:1943997/150‑1 (MQ=255)
                                    gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAAAAAATACAAAAGCGATGTAAAGCACAAAGt                                                                                                                  >  2:5051177/1‑150 (MQ=255)
                                     tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                            <  1:7171996/123‑1 (MQ=255)
                                     tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGACAAAAGCGATGTAAAGCACTAAGtt                                                                                                                 >  2:4337944/1‑150 (MQ=255)
                                     tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAACAAGAAAAAAGAGATGTAAAGGACTAAGtt                                                                                                                 >  1:7557947/1‑150 (MQ=255)
                                        tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                              <  1:3700211/150‑1 (MQ=255)
                                         ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                             <  1:6342059/150‑1 (MQ=255)
                                           ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                           <  2:5293930/150‑1 (MQ=255)
                                               gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                       <  2:8455227/150‑1 (MQ=255)
                                                ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                                                                                                      <  1:8136909/150‑1 (MQ=255)
                                                    ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAAACAAGACAAAAGCCAGGTAAAGCACAAAGTTTAAAATTCGcccccc                                                                                                  >  1:1867582/1‑148 (MQ=255)
                                                      atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTCAAAGAAATCAATACAAAAGCGATTTAAAGCACTAAGGTTAAATTTCGCGcccaaa                                                                                                >  2:1973860/1‑146 (MQ=255)
                                                         taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                             <  1:1736565/150‑1 (MQ=255)
                                                           gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                           <  2:3443714/150‑1 (MQ=255)
                                                           gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATAAAGACAATAGCGATGTAAATCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                           >  2:3627680/1‑150 (MQ=255)
                                                           gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTGTTTAAATTATAAAAAAATTAAAAAAAAAAAAAAAAAAAGACAGGTAAAACACAAAGATTAAATTTAGCGCCCAACACGgc                                                                                           >  1:3313238/1‑150 (MQ=255)
                                                             gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                         <  2:6556550/150‑1 (MQ=255)
                                                             gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                         <  1:8641165/150‑1 (MQ=255)
                                                              aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcg                                                                                        <  2:1554781/150‑1 (MQ=255)
                                                              aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGAAAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcg                                                                                        >  2:5247739/1‑150 (MQ=255)
                                                              aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATACAATAGCGATGTAAAGCACAAAGTTTAAATTTCGCGCCAATCATGgcgcg                                                                                        >  2:6862224/1‑150 (MQ=255)
                                                              aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAATAAAAAATAGATGTAAATCACGAAGGTTAAATTTGGCGCCCAACCAGgcgcg                                                                                        >  1:984463/1‑150 (MQ=255)
                                                              aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTAAAAAATAAATAAAATAAAAAAAGAAAAAATACAATTAAAGCACTAAAATTAAAATTCGCGCCAAACaattaacg                                                                                        >  2:3338764/1‑143 (MQ=255)
                                                              aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATAAAAGAAATAAAAACAATAAGGGGGGAAATCACGAAGTTTTAATTTCGCGCCCAACAGGgggcg                                                                                        >  1:7975939/1‑150 (MQ=255)
                                                               tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAAGAAAAAATCGATTTAAATCAATAATTTTAAATTTCGCGCCAAAAATGgcgcgc                                                                                       >  2:1820848/1‑150 (MQ=255)
                                                               tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAAAAATTAAAAGAAATAAAGACAAAATCGATTTAAATCAAAAAGTTTAAATTTCGCGCCAAACATTgagcgc                                                                                       >  2:3563388/1‑150 (MQ=255)
                                                                   agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                   <  2:7215636/150‑1 (MQ=255)
                                                                    gcaagcaTTTATTTGGAATGTATTTTTCGGTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTCAAATCAAACAAGACAATAGCGAGTTAAACCACAAAGGTGTCAATGGGCGCCACTCATGGGGCGcccacg                                                                                  >  2:4930933/1‑148 (MQ=255)
                                                                      aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAATAATTAAAAGAAAAAAAGAAAATAGCGATGTAAAGCAAAAAGTTTTAATTTCGCGCCAATCAAGGCGCGCACaacca                                                                                >  2:8618359/1‑147 (MQ=255)
                                                                          atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATATCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAAAAATGGCGCGCACAAGCTataa                                                                            >  2:1936557/1‑150 (MQ=255)
                                                                           tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataat                                                                           <  2:1639348/150‑1 (MQ=255)
                                                                              atttGTATTTTATTTTTCGTTCGACTTTTGTTTATAAATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATTTAAAGAACTATGTTTAAATTGCGCGCAAATCATGGCGCGCACAAGCTATAATAcc                                                                        <  1:4803012/150‑1 (MQ=255)
                                                                               tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCa                                                                       <  2:2274745/150‑1 (MQ=255)
                                                                               tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAAAAGCGATTTAAAGCACTAAGTTTAAATTTCGCGCCAAACATGGCGCGCACAAGCGATAATACCa                                                                       >  2:8924545/1‑150 (MQ=255)
                                                                                ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCACGCACAAGCTATAATACCaa                                                                      <  2:7169424/150‑1 (MQ=255)
                                                                                ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAACAATAAAAAAGAGAATTAAAGGACAAAATTTAAATTTATCGCCAATAATTGGGCGCACCAGCGATAATACCaa                                                                      >  2:8556164/1‑150 (MQ=255)
                                                                                ttGGAATGTATTTTTCGTTCGACCTGTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                      <  2:676108/150‑1 (MQ=255)
                                                                                 tgtaaTTTATTTTTCTTTCGACCTTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATTGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                     <  1:5788762/147‑1 (MQ=255)
                                                                                 tGGATTTTATTTTTCGTTCGACCTTTCTTTATAAATAACTGTGTTTTTTTTAATTTATAAAAAAATTCAAATAAATCAAGACATTCGCTTTGTAAAGAACTATTTTTAATTTGCGCGCCAATCAGGGCGCGCACAAGCTATAATACCAAc                                                                     <  1:7064077/150‑1 (MQ=255)
                                                                                 tGGAATTTATTTTTCTTTCGACATTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCGATGTAAAGCAATAAGTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                     <  1:1040335/150‑1 (MQ=255)
                                                                                     aTGTTTTTTTCGTTCGACCTTTGCTTATAAATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCTATGTAAAGAAATAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTaa                                                                 <  1:4338923/150‑1 (MQ=255)
                                                                                      tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                <  2:6126546/150‑1 (MQ=255)
                                                                                       gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATAATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                               >  2:1325914/1‑150 (MQ=255)
                                                                                          tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                            <  2:4427791/150‑1 (MQ=255)
                                                                                           ttttCGTTCTACCTTTGATTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATAATTACAATAGCTATTTAAACCACTATGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                           <  1:1709883/150‑1 (MQ=255)
                                                                                           ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                           <  2:7671617/150‑1 (MQ=255)
                                                                                                gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAAAAGCGAAGTAAATCACTAAGTTTAAATTGCGCGCCAAACATGGCGCGCACAAGCTATAATACCAACCTAATTTctccacca                                                      >  2:572891/1‑149 (MQ=255)
                                                                                                 ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATACAATAGCGATGTAAAGCACTAAGTTTAAATTTAGCGCCAATAATGGCGCGCACAAGCAATAAAAACAACCAAATTTctactcctc                                                     >  2:6366300/1‑150 (MQ=255)
                                                                                                 ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAAAAAGAAAAAATCGATGTTAATCACTAAGTTTAAATTTGGCGCCAAACATTGCGCGCACAAGCGATAATAACAACCAAAATTatcctcctc                                                     >  2:3956841/1‑150 (MQ=255)
                                                                                                     aCCTTTGCTTATACATAACAGGCGTTTGTTTAATTTATAAAATAATTAAAATTTATCAAGACAATATCGATGGAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACCAGCTATAATAACAAACAAATTTATCCTCCTCTTag                                                 >  2:725248/1‑150 (MQ=255)
                                                                                                      ccTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                <  1:1821061/150‑1 (MQ=255)
                                                                                                          tGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATCCAAAGAAATCAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCACCCTAATTTCTCCTCCTCTTAGAGTGa                                            <  1:1580593/150‑1 (MQ=255)
                                                                                                           gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                           <  1:8309041/150‑1 (MQ=255)
                                                                                                             ttATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                         <  1:2637424/150‑1 (MQ=255)
                                                                                                             ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                         <  1:2845706/150‑1 (MQ=255)
                                                                                                                     aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                 <  2:7999416/150‑1 (MQ=255)
                                                                                                                            gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                          >  1:8917089/1‑150 (MQ=255)
                                                                                                                             tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                         >  2:7481268/1‑150 (MQ=255)
                                                                                                                                ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                      <  2:2123236/150‑1 (MQ=255)
                                                                                                                                 tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                     <  1:5669430/150‑1 (MQ=255)
                                                                                                                                 tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                     <  1:6133662/150‑1 (MQ=255)
                                                                                                                                     aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                 >  1:2407635/1‑150 (MQ=255)
                                                                                                                                       ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg               <  2:4192069/150‑1 (MQ=255)
                                                                                                                                        tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa              >  1:442399/1‑150 (MQ=255)
                                                                                                                                        tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa              >  1:2129325/1‑150 (MQ=255)
                                                                                                                                         ataAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc             <  1:499336/150‑1 (MQ=255)
                                                                                                                                         ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc             <  2:5523657/150‑1 (MQ=255)
                                                                                                                                          taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt            >  1:5313486/1‑150 (MQ=255)
                                                                                                                                            aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTAGTGACTAt          >  1:4017368/1‑150 (MQ=255)
                                                                                                                                                aatTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa      <  2:831067/150‑1 (MQ=255)
                                                                                                                                                 atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt     >  2:5393155/1‑150 (MQ=255)
                                                                                                                                                   tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc   <  1:1991215/150‑1 (MQ=255)
                                                                                                                                                   tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc   >  1:708767/1‑150 (MQ=255)
                                                                                                                                                   tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc   >  1:5858417/1‑150 (MQ=255)
                                                                                                                                                    cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc  >  1:6699943/1‑150 (MQ=255)
                                                                                                                                                    cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc  <  1:3921614/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCC  >  NC_000913/2470279‑2470576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: