Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,436 C→A 12.5% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4360CA12.5% 1219.6 / 45.6 410Q436K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (131/211);  new base A (62/4);  total (195/215)
Fisher's exact test for biased strand distribution p-value = 1.77e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 418 total aligned reads displayed.

TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585
                                                                                                                                                    |                                                                                                                                                     
 ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATATAATAATTCAAAGAAATCaa                                                                                                                                                     <  1:5734562/150‑1 (MQ=255)
 ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                     <  2:5612675/150‑1 (MQ=255)
   ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                                   <  1:196107/150‑1 (MQ=255)
        gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                              <  2:8740213/150‑1 (MQ=255)
        gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                              <  1:1902470/150‑1 (MQ=255)
        gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                              >  2:680155/1‑150 (MQ=255)
         aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAg                                                                                                                                             >  2:2310645/1‑150 (MQ=255)
          atatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                                                                                                            <  1:6922189/150‑1 (MQ=255)
          atatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAAAAAAAAGAAAAAAAAACaaaaac                                                                                                                                            >  1:896790/1‑146 (MQ=255)
           tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                           <  2:9128671/150‑1 (MQ=255)
           tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGAAAAAAGCg                                                                                                                                           >  2:3434429/1‑150 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  2:1223555/150‑1 (MQ=255)
              ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAACAAGACAAAATCGATg                                                                                                                                        >  2:5226678/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                       <  2:4898334/150‑1 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAATCGATGt                                                                                                                                       >  2:2894313/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAACAAAACAAAATCGATGt                                                                                                                                       >  1:6729513/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATAAAGAAAAAAGCGATGt                                                                                                                                       >  2:5920639/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAATTCAAAGAAATCAAGACAAAAGAGATgg                                                                                                                                       >  1:2201882/1‑149 (MQ=255)
                  aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                                    <  1:7256970/150‑1 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGTATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                   <  2:7730318/150‑1 (MQ=255)
                    tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTTTTTATTAATTATTTTATAGTAATGAAGAGAATAGCGgtgttttgg                                                                                                                                  >  2:3703173/1‑145 (MQ=255)
                          tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAGCGATGTAAATCACAAAg                                                                                                                            >  2:3612404/1‑150 (MQ=255)
                          tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAAAAGCGATTTAAAGAACAAAg                                                                                                                            >  1:5834420/1‑150 (MQ=255)
                          tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATCAAGACAATAGCGATGGAAAGCACTAAg                                                                                                                            >  2:6594601/1‑150 (MQ=255)
                            tCATTTTTCGGGGTAGGAGATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                          <  1:7894643/150‑1 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGACAAAAGCGATGTAAAGCACTAAGtt                                                                                                                          >  2:4337944/1‑150 (MQ=255)
                            tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAATACAAAATCGATGTAAAGAACGAAAtt                                                                                                                          >  1:1997340/1‑150 (MQ=255)
                              aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCTTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAAAAAAAAAAAAAAAATCGAATTAAAGAACAAAGTTTa                                                                                                                        >  1:8453561/1‑150 (MQ=255)
                              aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTTAAAATAATTAAAAGAAAAAAATACAAAATCGATTTAAATCACTAAGTTTa                                                                                                                        >  1:7658815/1‑150 (MQ=255)
                                  ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                                    <  2:5293930/150‑1 (MQ=255)
                                    cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgc                                                                                                                  <  1:5604318/150‑1 (MQ=255)
                                     ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                                 <  2:5374426/150‑1 (MQ=255)
                                      gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                                <  2:3560157/150‑1 (MQ=255)
                                       ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                                                                                                               <  1:8136909/150‑1 (MQ=255)
                                       ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAACAAGACAATAGCGATGTAAAGCACTAACTTTAAAATtcgcc                                                                                                               >  2:9039830/1‑149 (MQ=255)
                                             atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTCAAAGAAATCAATACAAAAGCGATTTAAAGCACTAAGGTTAAATTTCGCGcccaaa                                                                                                         >  2:1973860/1‑146 (MQ=255)
                                                taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                      <  1:1736565/150‑1 (MQ=255)
                                                taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGACAAAAGCGATGTAAAACACGAATTTTAAATTTCGCGCCAAACaag                                                                                                      >  1:4412283/1‑148 (MQ=255)
                                                    gaTTTAAGCTAGGATTTATTTGGAATGTATCTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAATGAATTCAAGACAATAGCGATGTAAAGCTCTAAGTTTAAATTGCGCGCCAATGATGgcgc                                                                                                  <  2:5518900/150‑1 (MQ=255)
                                                     aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATAAAAGAAATAAAAACAATAAGGGGGGAAATCACGAAGTTTTAATTTCGCGCCCAACAGGgggcg                                                                                                 >  1:7975939/1‑150 (MQ=255)
                                                      tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAAATAAAATAAATAAAGAAAAAATCGATGTGAATCACAAATTTTATATTTGGGGCCGATAAGGgcgcgc                                                                                                >  1:5892250/1‑150 (MQ=255)
                                                      tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAAGAAAAAATCGATTTAAATCAATAATTTTAAATTTCGCGCCAAAAATGgcgcgc                                                                                                >  2:1820848/1‑150 (MQ=255)
                                                         aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATATAATAAAAAAATAAAAAAAAAAAAAACAAAAACCAAGTATAACACAAAATTTAAAAATAGCGCCAAACAAGgcgcccccc                                                                                             >  1:7017368/1‑145 (MQ=255)
                                                          agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                            <  2:7215636/150‑1 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAAAAAACATGTAAAAAACAAAGTTTAAATTTCGCGCCCCTAAAGGCGCGCACAAGcg                                                                                         >  2:1568615/1‑149 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAATAATTAAAAGAAAAAAAGAAAATAGCGATGTAAAGCAAAAAGTTTTAATTTCGCGCCAATCAAGGCGCGCACaacca                                                                                         >  2:8618359/1‑147 (MQ=255)
                                                               gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtat                                                                                       <  2:7326374/150‑1 (MQ=255)
                                                                 atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATATCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAAAAATGGCGCGCACAAGCTataa                                                                                     >  2:1936557/1‑150 (MQ=255)
                                                                  tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataat                                                                                    <  2:1639348/150‑1 (MQ=255)
                                                                    tatttGGAATGTTTTTTTCGTTCGACATTTTCTTATAAATAACTTTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAATACAATAGCGTTGTAAAGCACTATGTTTAACTTGCGCGCCAAACATGGCGCGCACAAGCTATAATAc                                                                                  <  1:4827469/150‑1 (MQ=255)
                                                                      tttGGATTGTATTTTTCGTTCGAACTTTGATTATACATAACTGTCGTTTTTTTAATTTATAAAATAATTCAAACAAATAAATACAATTGCGATTTAAATCACTATTTTTAAATTGCGCGCCATTCATGGCGCGCACAAGCTATAATACCa                                                                                <  1:3624506/150‑1 (MQ=255)
                                                                       ttGTAATTTATTTTTCGTTCGACCTTTGATTATAAATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                               <  1:6631383/150‑1 (MQ=255)
                                                                       ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAATCAAGAAAAAAGCGATGTAAATCACTAAGTTTAAATTTCGCGCCAATCATTGCGCGCACAAGCTATAATACCaa                                                                               >  2:2267949/1‑150 (MQ=255)
                                                                        tGGAATTTATTTTTCTTTCGACATTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCGATGTAAAGCAATAAGTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                              <  1:1040335/150‑1 (MQ=255)
                                                                          gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAACAAGACAAAAGCGATGTAAAGCACAAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCGATAATACCAACCt                                                                            >  2:1329620/1‑150 (MQ=255)
                                                                          gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAAGAAAATATCGATGTAAATCACAAAGTTTTAATTTAGCGCCAATAATTGCGCGCACAAGCTCAAATAACaaaca                                                                            >  2:3329168/1‑147 (MQ=255)
                                                                             tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                         <  2:6126546/150‑1 (MQ=255)
                                                                                 tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtttat                                                                     <  2:4427806/150‑3 (MQ=255)
                                                                                  ttttCGTTCTACCTTTGATTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATAATTACAATAGCTATTTAAACCACTATGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                    <  1:1709883/150‑1 (MQ=255)
                                                                                       gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAAAAGCGAAGTAAATCACTAAGTTTAAATTGCGCGCCAAACATGGCGCGCACAAGCTATAATACCAACCTAATTTctccacca                                                               >  2:572891/1‑149 (MQ=255)
                                                                                        ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAATTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCCCAATCAATAATACCAACCAAATTTctacacctc                                                              >  2:8417662/1‑150 (MQ=255)
                                                                                        ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATACAATAGCGATGTAAAGCACTAAGTTTAAATTTAGCGCCAATAATGGCGCGCACAAGCAATAAAAACAACCAAATTTctactcctc                                                              >  2:6366300/1‑150 (MQ=255)
                                                                                        ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAAAAAGAAAAAATCGATGTTAATCACTAAGTTTAAATTTGGCGCCAAACATTGCGCGCACAAGCGATAATAACAACCAAAATTatcctcctc                                                              >  2:3956841/1‑150 (MQ=255)
                                                                                             ccTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                         <  1:1821061/150‑1 (MQ=255)
                                                                                               tttGCGTATACATAACAGTCGTTTTTTTAATTCATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTGAAAGGGCGCGCCAAGCATGGCGCGCACAAGCTATAATACCAACCTAAGTTCTCCTCCTCTTAGAGt                                                       <  2:5040801/150‑1 (MQ=255)
                                                                                                 tGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATCCAAAGAAATCAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCACCCTAATTTCTCCTCCTCTTAGAGTGa                                                     <  1:1580593/150‑1 (MQ=255)
                                                                                                 tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGGGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCAGGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                     <  1:3820611/150‑1 (MQ=255)
                                                                                                 tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                     <  2:2073573/150‑1 (MQ=255)
                                                                                                  gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                    <  2:8059516/150‑1 (MQ=255)
                                                                                                  gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                    <  1:8309041/150‑1 (MQ=255)
                                                                                                   cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                                   <  2:8549810/150‑1 (MQ=255)
                                                                                                    ttATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                  <  1:2637424/150‑1 (MQ=255)
                                                                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                  <  1:2845706/150‑1 (MQ=255)
                                                                                                        aCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtc                                              >  2:753898/1‑150 (MQ=255)
                                                                                                           tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                           <  2:2727943/150‑1 (MQ=255)
                                                                                                              cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                        <  2:304715/150‑1 (MQ=255)
                                                                                                                   gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                   >  2:6871821/1‑150 (MQ=255)
                                                                                                                   gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                   >  1:3944476/1‑150 (MQ=255)
                                                                                                                    ttgttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                  >  1:1875275/4‑150 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGTTAGAATTGCg                              >  1:9077790/1‑150 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  1:3329168/150‑1 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  1:6133662/150‑1 (MQ=255)
                                                                                                                         ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                             >  1:5429101/1‑150 (MQ=255)
                                                                                                                          tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                            <  1:6305532/150‑1 (MQ=255)
                                                                                                                          tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                            >  2:7680855/1‑150 (MQ=255)
                                                                                                                            aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTCATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                          >  1:7087658/1‑150 (MQ=255)
                                                                                                                            aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                          >  1:2407635/1‑150 (MQ=255)
                                                                                                                              ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                        <  2:4192069/150‑1 (MQ=255)
                                                                                                                                ataAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                      <  1:499336/150‑1 (MQ=255)
                                                                                                                                 taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                     >  1:5313486/1‑150 (MQ=255)
                                                                                                                                      taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAGTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa                >  1:4606835/1‑150 (MQ=255)
                                                                                                                                          tCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATAGCGCGCCCCTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTCTATCTCCTGATAGACTTGCGGTAGTGACTCTCACATGc            >  1:472649/1‑150 (MQ=255)
                                                                                                                                           cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc           <  1:4146869/150‑1 (MQ=255)
                                                                                                                                           cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc           <  1:8681804/150‑1 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          <  1:4337944/150‑1 (MQ=255)
                                                                                                                                            aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc          >  1:1417676/1‑150 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         >  2:7048192/1‑150 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         >  2:6171045/1‑150 (MQ=255)
                                                                                                                                              aGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg        >  2:5213487/1‑150 (MQ=255)
                                                                                                                                                    cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt  <  1:3038384/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: