Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,718,314 A→C 5.2% V72G (GTT→GGT)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,718,3140AC 5.2% 1505.1 / 9.1 511V72G (GTT→GGT) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (293/176);  new base C (3/39);  total (296/215)
Fisher's exact test for biased strand distribution p-value = 7.21e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 541 total aligned reads displayed.

TCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAA  >  NC_000913/2718165‑2718463
                                                                                                                                                     |                                                                                                                                                      
 ctGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc                                                                                                                                                       <  1:4029978/150‑1 (MQ=255)
  tGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAcc                                                                                                                                                      <  2:3715927/150‑1 (MQ=255)
  tGGAAAAGTGCCTGACAAGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTATACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAcc                                                                                                                                                      >  2:5263899/1‑150 (MQ=255)
   ggAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                                                                                                    <  2:5940654/150‑1 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCgg                                                                                                                                                   >  2:6473273/1‑149 (MQ=255)
    gAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGAGCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                                                                                                   <  2:2174527/150‑1 (MQ=255)
     aaaaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTg                                                                                                                                                  >  2:7837755/1‑150 (MQ=255)
     aaaaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCAGTATCAATTAAACTTTTTCCACTGATGCCACCGTGATCGGACTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTg                                                                                                                                                  <  1:7715110/150‑1 (MQ=255)
         gTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGcc                                                                                                                                              >  1:3060947/1‑150 (MQ=255)
          tGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGACACCGGGCGccc                                                                                                                                             >  2:6169143/1‑149 (MQ=255)
              tGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGc                                                                                                                                         >  1:2773264/1‑150 (MQ=255)
                   tGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt                                                                                                                                   >  2:545129/1‑150 (MQ=255)
                       ttttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAACCCGTGCGCCACGGCGAATCTTcc                                                                                                                                >  2:6089353/1‑150 (MQ=255)
                             ccGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTg                                                                                                                          >  1:4641396/1‑150 (MQ=255)
                              cGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGc                                                                                                                         >  1:1011208/1‑150 (MQ=255)
                               gTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg                                                                                                                        >  2:501062/1‑150 (MQ=255)
                                   aCGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCa                                                                                                                    <  2:9131577/150‑1 (MQ=255)
                                   aCGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCa                                                                                                                    >  1:1967939/1‑150 (MQ=255)
                                     gcgGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACg                                                                                                                  >  2:6997159/1‑150 (MQ=255)
                                        ggTTTCTGCCCCACGCTGACCCGGCAACACTTCCGGAACAATAAAACTTTTACCAGTGATGCCACGGTGAGGGGCCTTTTCCGGCTTCTCATCACCCGGCGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg                                                                                                               <  2:3237781/150‑1 (MQ=255)
                                         gTTTCTTCCGCACGCTGACGACGCAACACTTCCGGACCAATAACACTTTTACCACTGATGCCACCGTGAGCGGCCTTTGCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGc                                                                                                              <  2:8336675/150‑1 (MQ=255)
                                          tttCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGACTCTTCCCACTTGCGGTCACGGGGCt                                                                                                             >  2:7540701/1‑150 (MQ=255)
                                          tttCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt                                                                                                             >  1:6453123/1‑150 (MQ=255)
                                             cttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc                                                                                                          >  2:2342946/1‑150 (MQ=255)
                                             cttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc                                                                                                          >  1:2332004/1‑150 (MQ=255)
                                              ttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct                                                                                                         >  1:620738/1‑150 (MQ=255)
                                              ttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct                                                                                                         >  2:5666862/1‑150 (MQ=255)
                                                  gcccGCTGACGCCGCAACCCGCCCGGATCAATAAAACTTTGACCCCGGAGGCCCCCGTGATCGGCCTTTCCCGGCGTCACACCACCCGGCGCGCGGGAAACGGTGCGCCACGGCGTATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt                                                                                                     <  2:8391803/147‑1 (MQ=255)
                                                  gCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt                                                                                                     >  1:4183209/1‑150 (MQ=255)
                                                    aCGCTGACCACGCAACACTTCGGCAAAATTAAAACTTTTACCCCTGATGCCACGGTGTGGGGCTTTTACCGGCTTCTAATCCCCCGGGGGGGGGTAAACCGGGGGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt                                                                                                   <  2:6293068/150‑1 (MQ=255)
                                                      gCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGt                                                                                                 >  1:8188716/1‑150 (MQ=255)
                                                               caaaCACTCCCGTAAAAAAAAAACTTTTACCACTTTGGCCACGGTTATCGGCATTTGCCGGCGTCAAAACCCCCGGCGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcggg                                                                                        <  1:5661225/148‑1 (MQ=255)
                                                                 aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                      >  1:7906165/1‑150 (MQ=255)
                                                                   cacTTCCGGAAAAAAAAAACTTTTACCACTGACGCCCCCGTGATCGGCCTTTTCGGGCGTCTCACCCCCGGGCGCGCGGCAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg                                                                                    <  1:1458502/150‑1 (MQ=255)
                                                                   cacTTCCGCACAAAAAAAACTTTCCCCCCTGTTCCCACCGTGATCGGCCTTTGCGGGCGTCTCACCACCGGGGGCGGGGGAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg                                                                                    <  1:5491409/150‑1 (MQ=255)
                                                                   cacGTCCGGATCAATAAAACTTTTACCCCTGATGCCACCGTTCTGGGCCTTTTCCGGCGTCTCATCCCCCGGCGGGCGGTAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg                                                                                    <  1:9009385/150‑1 (MQ=255)
                                                                    ccTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcgggg                                                                                   <  2:3106438/149‑1 (MQ=255)
                                                                     cTTCCGGAACAATAAAACTTTTACCCCTGATGCCACCGTGATCGGCCTTTTCGGGCGTCTCATCACCCGGCGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                                                  <  1:5980615/150‑1 (MQ=255)
                                                                      ttCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGa                                                                                >  1:1293515/1‑150 (MQ=255)
                                                                         cgtaacAAAAAAACTTTCACCCCTGAGGCCACGGTGTTCGGCCTTTTCGGGCGTCACACCCCCCGGCGCGGGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACg                                                                              <  1:294003/145‑1 (MQ=255)
                                                                          ggacaaaTAAAACTTTCACCCCGGAGCCCACCGGGAGCGGCCTTTTCCGGCGTCACACCCCCCGGCGCGGGGCAAACCGCGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                             <  2:178254/145‑1 (MQ=255)
                                                                          ggaaaaaTAAAACTTTTACCACTGCTGCCACGGTGATCGGCCTTTTCCGGCGTCTCACCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                             <  2:7382812/145‑1 (MQ=255)
                                                                          ggATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                             >  2:5612522/1‑150 (MQ=255)
                                                                          ggATAAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                             <  2:1342402/150‑1 (MQ=255)
                                                                          gcataaaTAAAACTTTTCCCACTGAGGCCACCGTTATCGGCCTTTACCGGCGTCTAATCACCCGGCGCGCGGGAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                             <  1:680980/145‑1 (MQ=255)
                                                                            aTCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGcc                                                                           >  1:3736002/1‑150 (MQ=255)
                                                                            aTCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGcc                                                                           >  1:6904054/1‑150 (MQ=255)
                                                                              cAAAAAAACTTTTACCACTGAGGCCACCGTTATGGGCCTTTCCGGGCGTCTCATCACCCGGGGCGCGGGAACCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                                                         <  2:1129860/150‑1 (MQ=255)
                                                                               aaTAAAACTTTTCCCACTGATCCCACCGTGATCGGCCTTTGCCGGCGTCTCATCCCCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                        <  2:3359762/150‑1 (MQ=255)
                                                                               aaTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                        >  2:2768252/1‑150 (MQ=255)
                                                                                aTAAAACTTTTACCCCTGAGGCCACCGTGATCGGCCTTTCCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                       <  2:9002306/150‑1 (MQ=255)
                                                                                aTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                       >  2:8503496/1‑150 (MQ=255)
                                                                                aTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                                                       >  2:2461717/1‑150 (MQ=255)
                                                                                 tAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGt                                                                      >  2:3038882/1‑150 (MQ=255)
                                                                                 tAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGt                                                                      >  2:7188724/1‑150 (MQ=255)
                                                                                      cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCa                                                                 >  2:4415423/1‑150 (MQ=255)
                                                                                        ttaaCCACTGATCCCCCCGTGATCGGCCTTGACCGGCGTCACAACCCCCGGCGCGCGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACg                                                               <  1:3596767/147‑1 (MQ=255)
                                                                                         ttACCACTGATGCCACCGTTATGGGCCTTGTCGGGCGTCTCATCCCCCGGCGCGCGGGACCCGGTGGGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACga                                                              <  2:2569922/150‑1 (MQ=255)
                                                                                         ttACCACTGATGCCACCGTTATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACga                                                              <  1:5312358/150‑1 (MQ=255)
                                                                                         ttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACga                                                              >  2:530632/1‑150 (MQ=255)
                                                                                           aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCAACACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACgaga                                                            <  2:4557279/150‑1 (MQ=255)
                                                                                               cTGATGCCACGGTGTTCGGCCTTTTCCGGCGTCACATCCCCCGGCGCGCGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                        <  1:7113791/150‑1 (MQ=255)
                                                                                               cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                        >  2:8662335/1‑150 (MQ=255)
                                                                                                tGATGCCACCGTGAGCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                       <  1:1831846/150‑1 (MQ=255)
                                                                                                tGAGGCCACGTTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGGGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                       <  2:1011208/150‑1 (MQ=255)
                                                                                                 gaggCCACGGTGATCGGCCTTTTCCGGCGTCTCAACACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTcc                                                      <  2:3580769/147‑1 (MQ=255)
                                                                                                  aggCCACCGTGATCGGCCTTTTCCGGCGTCTCACCCCCCGGCGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCt                                                     <  2:1967939/148‑1 (MQ=255)
                                                                                                  aTGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCt                                                     >  1:209243/1‑150 (MQ=255)
                                                                                                  aTGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCt                                                     >  1:3542779/1‑150 (MQ=255)
                                                                                                  aTGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCt                                                     >  1:8456058/1‑150 (MQ=255)
                                                                                                      cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAc                                                 >  2:8754198/1‑150 (MQ=255)
                                                                                                         cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcc                                              >  2:6593227/1‑150 (MQ=255)
                                                                                                             ttCGGCCTTTGCCGGGGTCTCACCACCCGGCGCGCGGTAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgcc                                          <  1:8821614/149‑1 (MQ=255)
                                                                                                             aTCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgcc                                          >  1:6658662/1‑150 (MQ=255)
                                                                                                                  ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                     >  2:4009287/1‑150 (MQ=255)
                                                                                                                    ttttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc                                   >  2:4075016/1‑150 (MQ=255)
                                                                                                                      ttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTccc                                 >  1:7226080/1‑150 (MQ=255)
                                                                                                                         cGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGt                              >  2:4251639/1‑150 (MQ=255)
                                                                                                                          ggCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTa                             <  2:2869039/150‑1 (MQ=255)
                                                                                                                            cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcg                           <  2:1392976/150‑1 (MQ=255)
                                                                                                                                tcatcaCCCGGCGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                       <  2:7294632/150‑1 (MQ=255)
                                                                                                                                tcatcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                       >  1:7399794/1‑150 (MQ=255)
                                                                                                                                tcatcaCCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                       <  1:2212615/150‑1 (MQ=255)
                                                                                                                                 catcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                      >  2:2065856/1‑150 (MQ=255)
                                                                                                                                  accccccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                     <  1:4391279/146‑1 (MQ=255)
                                                                                                                                     aCCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgtg                  <  2:1237534/150‑1 (MQ=255)
                                                                                                                                     aCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgtg                  <  2:5069285/150‑1 (MQ=255)
                                                                                                                                       ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTggg                >  1:6506571/1‑150 (MQ=255)
                                                                                                                                          gcgcgcGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTAc             <  2:5860925/150‑1 (MQ=255)
                                                                                                                                              gcgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGttt         >  1:2574167/1‑150 (MQ=255)
                                                                                                                                              cggggTAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGtt          <  2:4922155/148‑1 (MQ=255)
                                                                                                                                                 ggAACCCGTGCGCCACTGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTGCCCGGTACGCGGGTTGTGGGTACGTTTATc      <  1:5227883/150‑1 (MQ=255)
                                                                                                                                                 ggAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc      <  1:4837506/150‑1 (MQ=255)
                                                                                                                                                  gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                        >  1:838077/1‑83 (MQ=255)
                                                                                                                                                  gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa     <  2:476965/150‑1 (MQ=255)
                                                                                                                                                    aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa   >  1:3354063/1‑150 (MQ=255)
                                                                                                                                                    aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa   >  1:8026828/1‑150 (MQ=255)
                                                                                                                                                    aaCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa   <  1:2543176/150‑1 (MQ=255)
                                                                                                                                                     aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGaa  <  2:8103599/150‑1 (MQ=255)
                                                                                                                                                     aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGaa  <  2:4641396/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAA  >  NC_000913/2718165‑2718463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: