Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,956 T→C 6.5% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9560TC 6.5% 1401.2 / 55.3 488intergenic (+354/+260)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base T (111/320);  new base C (16/14);  total (150/338)
Fisher's exact test for biased strand distribution p-value = 2.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 493 total aligned reads displayed.

CGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGG  >  NC_000913/3269807‑3270105
                                                                                                                                                     |                                                                                                                                                     
cgccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGt                                                                                                                                                       <  1:1404017/150‑1 (MQ=255)
  ccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaa                                                                                                                                                     >  1:8216836/1‑150 (MQ=255)
   cgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                                                                                                                    <  2:6701969/150‑1 (MQ=255)
   cgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                                                                                                                    <  1:3329418/150‑1 (MQ=255)
     cATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaaaa                                                                                                                                                  <  2:1853998/150‑1 (MQ=255)
      aTCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAc                                                                                                                                                 <  2:5190796/150‑1 (MQ=255)
        ccGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAccc                                                                                                                                               <  1:5962524/150‑1 (MQ=255)
         cGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccg                                                                                                                                              <  2:3144901/150‑1 (MQ=255)
          ggCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgc                                                                                                                                             <  2:609434/150‑1 (MQ=255)
           gCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgcc                                                                                                                                            <  2:9124884/150‑1 (MQ=255)
               gAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAg                                                                                                                                        <  2:8123565/150‑1 (MQ=255)
               gAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAg                                                                                                                                        <  2:6892329/150‑1 (MQ=255)
               gAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAg                                                                                                                                        >  1:8110160/1‑150 (MQ=255)
                aaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGc                                                                                                                                       <  1:7216133/150‑1 (MQ=255)
                  tAAAGCGCACTCAACAATCTGAAACCCGCCGGATCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                     <  2:7025093/150‑1 (MQ=255)
                  tAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                     >  1:7909440/1‑150 (MQ=255)
                  tAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                     <  2:2485807/150‑1 (MQ=255)
                    aaGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGt                                                                                                                                   >  1:6936841/1‑150 (MQ=255)
                     aGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtt                                                                                                                                  <  2:6602315/150‑1 (MQ=255)
                      gcgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttt                                                                                                                                 <  2:240899/150‑1 (MQ=255)
                       cgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtttt                                                                                                                                <  1:2149611/150‑1 (MQ=255)
                       cgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtttt                                                                                                                                <  1:4991543/150‑1 (MQ=255)
                           cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCACCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTaag                                                                                                                            >  2:1200645/1‑148 (MQ=255)
                            tCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                           >  2:3460644/1‑150 (MQ=255)
                               acaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                        <  2:3751462/150‑1 (MQ=255)
                                caaTCTGAAACCCGCCGTATCGGTTTATTACGTACAGCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAATAACCCGCCGAAGCGGGTTTTTACGTaaat                                                                                                                       <  2:6414790/150‑2 (MQ=14)
                                 aaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAc                                                                                                                      >  1:8676058/1‑150 (MQ=255)
                                  aTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACa                                                                                                                     >  2:2795134/1‑150 (MQ=255)
                                  aTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACa                                                                                                                     <  2:294466/150‑1 (MQ=255)
                                   tCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAg                                                                                                                    <  2:4527327/150‑1 (MQ=255)
                                   tCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAg                                                                                                                    <  2:889176/150‑1 (MQ=255)
                                     tGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGt                                                                                                                  <  2:2037241/150‑1 (MQ=255)
                                       aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACAGGTGa                                                                                                                >  2:4068013/1‑150 (MQ=255)
                                        aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                               >  2:4938016/1‑150 (MQ=255)
                                        aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaa                                                                                                               >  2:7700436/1‑150 (MQ=255)
                                          cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAc                                                                                                             <  2:2564972/150‑1 (MQ=255)
                                             gccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                          >  1:6184163/1‑150 (MQ=255)
                                              ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                         <  1:1228814/150‑1 (MQ=255)
                                              ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                         <  1:1542270/150‑1 (MQ=255)
                                               cgtaccGGTTTATTACGTACAACAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAcc                                                                                                        <  2:1373863/145‑1 (MQ=11)
                                                  aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                             <  2:5089796/126‑1 (MQ=255)
                                                     ggTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAg                                                                                                  <  1:6620544/150‑1 (MQ=255)
                                                      gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc                                                                                                 <  1:4245994/150‑1 (MQ=255)
                                                          attaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGct                                                                                             <  1:1006817/150‑1 (MQ=25)
                                                             aCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                          <  2:750657/150‑1 (MQ=255)
                                                              cGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttct                                                                                         <  2:8391038/150‑1 (MQ=255)
                                                                tACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                       <  1:3651715/150‑1 (MQ=40)
                                                                tACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                       <  2:4149081/150‑1 (MQ=40)
                                                                  cATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                     <  2:8261410/150‑1 (MQ=39)
                                                                   aTCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                    <  1:8504896/150‑1 (MQ=38)
                                                                    tCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                   >  2:383563/1‑150 (MQ=38)
                                                                     cAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt                                                                                  <  2:2725791/150‑1 (MQ=37)
                                                                             aCTGACCGATAAGCCTCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTc                                                                          <  1:6347597/150‑1 (MQ=37)
                                                                               tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAACCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGAAAAGCCGCGTTGTTTTTGGTGTAGGGTCGt                                                                        >  2:1581596/1‑150 (MQ=11)
                                                                                ttccGATCTGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                       <  1:2478495/148‑1 (MQ=255)
                                                                                 aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTgg                                                                      <  1:3365337/150‑1 (MQ=37)
                                                                                  ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGa                                                                     <  1:2422329/150‑1 (MQ=37)
                                                                                  ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGa                                                                     <  2:5785960/150‑1 (MQ=37)
                                                                                    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACa                                                                   <  1:1917836/150‑1 (MQ=37)
                                                                                    gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACa                                                                   <  2:6308365/150‑1 (MQ=37)
                                                                                         gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcat                                                              <  2:7880990/150‑1 (MQ=37)
                                                                                          ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAATAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGCTTTCTTTTTTGTATAGTGAAGAGGAAAGtcaat                                                             >  2:8471452/1‑148 (MQ=11)
                                                                                            gCTTTCTTTCGGGGTGTGTGTCGTGGACTGGGGTTCTTCTTTCGGGCCCTCCACAAGTTGAAACCCGCCGTAGCGGGTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGtcattca                                                           <  2:2262968/150‑1 (MQ=11)
                                                                                              tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATc                                                         <  1:3814339/150‑1 (MQ=37)
                                                                                                tctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCtt                                                       <  2:3516371/150‑1 (MQ=37)
                                                                                                 ctttTGGGTATAGTGTCGTGGACAGTCATTGATCTTTCTGCCCCTCCACCAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCttt                                                      <  1:6684494/150‑1 (MQ=255)
                                                                                                  ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTc                                                     <  1:797515/150‑1 (MQ=37)
                                                                                                   tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCt                                                    <  1:7709577/150‑1 (MQ=37)
                                                                                                   tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAACGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtat                                                                                <  2:4702311/122‑1 (MQ=25)
                                                                                                       ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGccc                                                <  2:4757534/150‑1 (MQ=37)
                                                                                                         tataTTGTCGTTGACAGACATTAATCTTTCTTCCCCTCCAAAAGTAAAAACCCGCCGAAGCGTGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCt                                              <  1:2028604/150‑1 (MQ=11)
                                                                                                         tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                    >  2:5395187/1‑112 (MQ=35)
                                                                                                          ataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                             <  1:2795134/150‑1 (MQ=37)
                                                                                                           taGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                            <  1:4666405/150‑1 (MQ=37)
                                                                                                              tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaa                                         <  1:8810502/150‑1 (MQ=37)
                                                                                                               gtCGTTGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                        <  1:5475629/150‑1 (MQ=37)
                                                                                                               gtCGTGGACTGTCAGTCTTCTTTCTGCCCCTCCAACAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                        <  1:4022598/150‑1 (MQ=255)
                                                                                                               gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                        <  1:5864878/150‑1 (MQ=37)
                                                                                                               gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                        <  1:5195228/150‑1 (MQ=37)
                                                                                                                  gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCa                                     >  1:894309/1‑150 (MQ=37)
                                                                                                                  gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCa                                     <  1:7409051/150‑1 (MQ=37)
                                                                                                                  gTGGACAGTCATTCATCTTTCTGACCCTCCAAAAGAAAAAACCCGACGAAGCGGGTATTTACGTAAAACAGGTGAAACTGAACGATAAGCCGATTTCTGTGGGGGATAGAGTCGGGGACAGTCATTCAGCTTTCTGCCGCTCCAGAAGCa                                     >  1:2904285/1‑150 (MQ=11)
                                                                                                                    ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                   <  1:3576201/150‑1 (MQ=37)
                                                                                                                    ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                   <  1:3064987/150‑1 (MQ=37)
                                                                                                                          tcattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                     >  1:507243/1‑46 (MQ=38)
                                                                                                                          tcattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                             <  1:255187/150‑1 (MQ=37)
                                                                                                                          tcattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                                                >  1:5721951/1‑115 (MQ=17)
                                                                                                                            attcatCTTTCTGCCCCTCCAACAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                           <  2:8006903/150‑1 (MQ=37)
                                                                                                                             ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGGGGAGAGTCCAGCATCATGCTGCCCCTCCAAAACCAAAAACccgccg                          >  2:5607957/1‑150 (MQ=11)
                                                                                                                                atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                       >  2:8802536/1‑150 (MQ=37)
                                                                                                                                 tCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATaa                                                                                                   >  1:16288/1‑73 (MQ=39)
                                                                                                                                   tttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                    <  2:7161834/150‑1 (MQ=37)
                                                                                                                                       tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt                                                                        >  2:7298541/1‑94 (MQ=40)
                                                                                                                                       tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                <  2:3921513/150‑1 (MQ=37)
                                                                                                                                           ccTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACACGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc            <  1:4375890/150‑1 (MQ=255)
                                                                                                                                           ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc            <  2:8930029/150‑1 (MQ=37)
                                                                                                                                              ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt               <  1:482588/144‑1 (MQ=37)
                                                                                                                                                aaaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa       >  2:4394532/1‑150 (MQ=37)
                                                                                                                                                 acaGTACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc      <  2:3312940/148‑1 (MQ=12)
                                                                                                                                                     tAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg  >  1:5313799/1‑150 (MQ=38)
                                                                                                                                                     |                                                                                                                                                     
CGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGG  >  NC_000913/3269807‑3270105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: