Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,537,327 G→T 5.5% intergenic (+39/‑58) greB → / → yhgF transcript cleavage factor/putative transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,3270GT 5.5% 1765.8 / 10.6 531intergenic (+39/‑58)greB/yhgFtranscript cleavage factor/putative transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base G (222/261);  new base T (46/2);  total (268/263)
Fisher's exact test for biased strand distribution p-value = 1.30e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 573 total aligned reads displayed.

CCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGG  >  NC_000913/3537178‑3537476
                                                                                                                                                     |                                                                                                                                                     
ccGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTg                                                                                                                                                       >  1:3024907/1‑150 (MQ=255)
 cGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATTGCTGGCAAATTTgc                                                                                                                                                      >  2:7125296/1‑150 (MQ=255)
 cGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgc                                                                                                                                                      <  1:6778616/150‑1 (MQ=255)
 cGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCAATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgc                                                                                                                                                      <  2:8349989/150‑1 (MQ=255)
   aTGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgcca                                                                                                                                                    <  1:729000/150‑1 (MQ=255)
   aTGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgcca                                                                                                                                                    <  2:6659993/150‑1 (MQ=255)
      gCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCAAAAATgccagcc                                                                                                                                                 <  2:8828432/150‑1 (MQ=255)
        ccGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGccagccag                                                                                                                                               >  2:5036036/1‑150 (MQ=255)
           cgcATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTcc                                                                                                                                            <  2:4496145/150‑1 (MQ=255)
             cATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGt                                                                                                                                          <  2:7877675/150‑1 (MQ=255)
              aTTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGta                                                                                                                                         <  1:8241815/150‑1 (MQ=255)
                 gCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATaa                                                                                                                                      <  2:7561446/150‑1 (MQ=255)
                   tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCGGATaaaa                                                                                                                                    >  1:7813385/1‑148 (MQ=255)
                   tGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACt                                                                                                                                    >  1:6481631/1‑150 (MQ=255)
                     aaaaaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTaa                                                                                                                                  >  1:1040484/1‑149 (MQ=255)
                       aaaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATcc                                                                                                                                >  1:4084923/1‑150 (MQ=255)
                       aaaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATcc                                                                                                                                >  2:5358735/1‑150 (MQ=255)
                        aaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATccc                                                                                                                               >  2:1788555/1‑150 (MQ=255)
                        aaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATccc                                                                                                                               >  2:7679210/1‑150 (MQ=255)
                          agaagTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCCTTTTTGCCAGCCAGGCCGTATAACTATCCCCt                                                                                                                             >  2:4290712/1‑150 (MQ=255)
                               tCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTCTCCCCTGAttt                                                                                                                        >  2:9168048/1‑150 (MQ=255)
                                 ggCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCGTATAACAATCCCCAGAttttt                                                                                                                      >  1:6642333/1‑150 (MQ=255)
                                 ggCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAttttt                                                                                                                      >  2:3067540/1‑150 (MQ=255)
                                 ggCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAttttt                                                                                                                      <  1:1590307/150‑1 (MQ=255)
                                 ggCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAttttt                                                                                                                      <  1:6274625/150‑1 (MQ=255)
                                  gCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGGTGGGATTTTTTCCAGCCAGTCAGTATTACTATACCCAGAtttttt                                                                                                                     >  1:5793583/1‑149 (MQ=255)
                                  gCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCACTCCGTATAACAATACCCAGAtttttt                                                                                                                     >  1:1113737/1‑149 (MQ=255)
                                  gCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCCTATAACTATCCCCAGAttttga                                                                                                                     >  2:2729570/1‑148 (MQ=255)
                                   cGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGa                                                                                                                    <  2:6488265/150‑1 (MQ=255)
                                     aTCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCCGTCCGTATAACTATACCCAGATTTTTGTTc                                                                                                                  >  1:1324991/1‑150 (MQ=255)
                                     aTCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATc                                                                                                                  <  1:3872615/150‑1 (MQ=255)
                                       cTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCg                                                                                                                <  2:3850254/150‑1 (MQ=255)
                                          gCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCGGGCATTTTTGCCAGCCACTCCGTATAACTATCCCCAGATTTTTGATACGaaa                                                                                                             >  2:8594660/1‑150 (MQ=255)
                                            ggtggtGTATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAg                                                                                                           <  2:3338085/150‑1 (MQ=255)
                                            ggtggtGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCAAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAg                                                                                                           >  2:5241198/1‑150 (MQ=255)
                                              tggtgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTACAGCCAGTACGTATAACTATACCCAGATTTTTTATAAGAAAAGAt                                                                                                         >  1:5559623/1‑150 (MQ=255)
                                                gtgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCGTATAAAAATCCCCAGATTTTTGTTCCGAAAAGATGa                                                                                                       >  2:3422608/1‑150 (MQ=255)
                                                gtgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGa                                                                                                       <  1:4968275/150‑1 (MQ=255)
                                                gtgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGa                                                                                                       <  2:8018491/150‑1 (MQ=255)
                                                 tgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGaa                                                                                                      <  1:3970421/150‑1 (MQ=255)
                                                 tgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGaa                                                                                                      <  2:7024747/150‑1 (MQ=255)
                                                     tACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCGTATAACTATCCCCAGATTTTTGATACGAAAAGATGAACTCa                                                                                                  >  2:289879/1‑150 (MQ=255)
                                                     tACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCACTCCGTATAACAAAACCCAGATTTTTTATCCGAAAAGATGAAcaca                                                                                                  >  1:716947/1‑150 (MQ=255)
                                                      aCCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCaa                                                                                                 <  1:4477891/150‑1 (MQ=255)
                                                      aCCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAAATATACCCAGATTTTTGTTCCGAAAAGATGAACTCaa                                                                                                 >  2:49224/1‑150 (MQ=255)
                                                           tGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCat                                                                                            <  1:3379933/150‑1 (MQ=255)
                                                           tGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCat                                                                                            <  1:1952084/150‑1 (MQ=255)
                                                            gCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCACTCCGTATAAATAACCCCAGATTTTTGTTCCGCAAAGATGAACTCAAAACatt                                                                                           >  2:4074549/1‑149 (MQ=255)
                                                                 ggAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGatgaa                                                                                      <  2:1305644/150‑1 (MQ=255)
                                                                  gAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCTTTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGatgaat                                                                                     <  1:1828485/150‑1 (MQ=255)
                                                                    aGCGAGCTGGTATGTTTATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGatgaatga                                                                                   <  2:8464532/150‑1 (MQ=255)
                                                                      cGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGAtt                                                                                 <  2:8145490/150‑1 (MQ=255)
                                                                           tGGTATGTTTATGCTATCTAGTACGTGAAACCGTAAGGAATAGTATTATCCTCCTGCCGATGGATGGCATTTTTGCCAGCCAGTCCTTATAACTATCACCTGATTTTTGATCCGAAATGATGAACTCAAACCATGATGAATGATTCGTTc                                                                            <  2:1059920/150‑1 (MQ=255)
                                                                           tGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGGATAACAATACCCAGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATTATTCGTTc                                                                            >  2:1633467/1‑150 (MQ=255)
                                                                             gTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATTGCTGGCAAGTTTGACAGCCAGGCCGTATAAATAAACCCAGATATTTGTTCAGAAAAGATGAACTAAAAACAAGAAGAATGATTAGTTcag                                                                          >  2:4363347/1‑148 (MQ=255)
                                                                             gTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTg                                                                          >  2:7212019/1‑150 (MQ=255)
                                                                              tATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCAAGATGAATGATTCGTTCTGc                                                                         >  2:8241333/1‑150 (MQ=255)
                                                                               aTGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGcc                                                                        <  1:2559521/150‑1 (MQ=255)
                                                                                tGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCg                                                                       <  1:2275164/150‑1 (MQ=255)
                                                                                  ttAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGTTGAACTCAAACCATGATGAATGATTCGTTCTGCCGCa                                                                     >  2:6771601/1‑150 (MQ=255)
                                                                                  ttAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGAGGGCGGGGATTTTTTGCAGCCACTCCGGATAAATAAACCCGGGTTTTTGTTGCGAGAAGATGTAATAAAACCAAGGTGGATGTTTGGTTCTGCCGcg                                                                     >  1:1759223/1‑149 (MQ=255)
                                                                                         tATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGc                                                              <  1:8588651/150‑1 (MQ=255)
                                                                                         tATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAAATATACCCGGATTTTTGATCCGCAAAGATTAACTCAAACCATGATGAATTATTCGTTCTGCCGCATTATTGc                                                              >  2:31576/1‑150 (MQ=255)
                                                                                            cGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCggg                                                           <  1:1706604/150‑1 (MQ=255)
                                                                                              aGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTg                                                         <  1:7125296/150‑1 (MQ=255)
                                                                                                tACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGaa                                                       <  1:5036438/150‑1 (MQ=255)
                                                                                                  cGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgc                                                                                                                                                      <  1:524544/53‑1 (MQ=255)
                                                                                                     gAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCa                                                  <  1:7938474/150‑1 (MQ=255)
                                                                                                       aaCCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAgg                                                <  1:2506699/150‑1 (MQ=255)
                                                                                                           gTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCAGGATGAATGATTCGTTCTGCCGCCTTATTGCGGGTGAAATTCAGgcgcg                                            >  2:5652830/1‑150 (MQ=255)
                                                                                                            tAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGgcgcgc                                           >  2:6569723/1‑150 (MQ=255)
                                                                                                               ggAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCg                                        >  2:1982555/1‑150 (MQ=255)
                                                                                                                gAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCCGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATTAACTCAAACCCTTATGTATGATTCGTTGTGGCGCCTTATTGCGGGTGAAATTCAGGCGCGCCCgg                                       >  2:5487376/1‑150 (MQ=255)
                                                                                                                 aaGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATTAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGa                                      >  2:5127300/1‑150 (MQ=255)
                                                                                                                    agTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACa                                   <  1:4704114/150‑1 (MQ=255)
                                                                                                                      tCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCCTATAACTATACCCAGATTTTTGATCCGAAAAGATGAACTCAAAACATGATGAATGATTGGTTGTGCCGCATTATTGCGGGTGAAATTAAGGCGCGCCCGGAACAgg                                 >  2:2496689/1‑150 (MQ=255)
                                                                                                                          aaCCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGa                             <  2:6881735/150‑1 (MQ=255)
                                                                                                                            cctcctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACg                           <  2:1553683/150‑1 (MQ=255)
                                                                                                                               cctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTg                        <  1:3730306/150‑1 (MQ=255)
                                                                                                                                   ggactggctggcAAAAATGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGt                    >  2:8693487/5‑150 (MQ=255)
                                                                                                                                    cGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGtt                   >  2:8966043/1‑150 (MQ=255)
                                                                                                                                    cGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCAGGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGtt                   >  2:628874/1‑150 (MQ=255)
                                                                                                                                    cGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTGTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGACCAGGTTGACGCTGCCGta                   >  2:6170168/1‑149 (MQ=255)
                                                                                                                                      aTGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCg                 >  2:2264047/1‑150 (MQ=255)
                                                                                                                                       tggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGc                <  1:2872139/150‑1 (MQ=255)
                                                                                                                                       tggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGc                <  1:8961748/150‑1 (MQ=255)
                                                                                                                                        ggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCATACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGcc               <  2:6008571/150‑1 (MQ=255)
                                                                                                                                          ctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTg             <  1:4595830/150‑1 (MQ=255)
                                                                                                                                           tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGcc               >  2:6430500/1‑147 (MQ=255)
                                                                                                                                           tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc            >  2:2983546/1‑150 (MQ=255)
                                                                                                                                           tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc            >  2:3531007/1‑150 (MQ=255)
                                                                                                                                               aTTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGa        >  1:7508464/1‑150 (MQ=255)
                                                                                                                                               aTTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGa        >  1:845040/1‑150 (MQ=255)
                                                                                                                                               aTTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGa        >  2:219265/1‑150 (MQ=255)
                                                                                                                                                tttttGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGAc       >  2:5796610/1‑150 (MQ=255)
                                                                                                                                                 aaatGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACg      >  1:7344913/4‑150 (MQ=255)
                                                                                                                                                  tttGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGa     >  2:4759544/1‑150 (MQ=255)
                                                                                                                                                    tGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAg   <  2:7405745/150‑1 (MQ=255)
                                                                                                                                                    tGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAg   >  2:3636969/1‑150 (MQ=255)
                                                                                                                                                    tGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGACGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAg   <  1:5383245/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGG  >  NC_000913/3537178‑3537476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: