Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,695 C→G 8.8% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,6950CG 8.8% 1651.6 / 37.8 517intergenic (+46/+56)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (290/171);  new base G (2/51);  total (292/225)
Fisher's exact test for biased strand distribution p-value = 3.62e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 542 total aligned reads displayed.

CCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATA  >  NC_000913/3608546‑3608844
                                                                                                                                                     |                                                                                                                                                     
 cacGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctct                                                                                                                                                      >  2:1728043/1‑150 (MQ=255)
  acGCTGTTCGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTGGCGCAGGAGATAAGGCAAACCGATCGCAACATTGCGCGCGATCGGTCCCCTCGCCCCTCTg                                                                                                                                                     <  1:4642345/150‑1 (MQ=255)
    gcggTTAGGGCTGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  2:7866271/147‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  2:9030744/150‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  2:8888503/150‑1 (MQ=255)
       gTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGgag                                                                                                                                                <  2:2569364/150‑1 (MQ=255)
         tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                              <  1:5113173/150‑1 (MQ=255)
         gaGGGAGGCCCGGGTTGGGGCGGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACCGCGAAGGCGTTAAGATTGTGGCGCAGGAGAGAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                              <  2:341595/149‑1 (MQ=255)
          aGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                                                                             <  1:9116919/150‑1 (MQ=255)
          aGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                                                                             >  1:5801744/1‑150 (MQ=255)
               tGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                                                                                        >  2:2375239/1‑150 (MQ=255)
                gACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                                       <  1:3567978/150‑1 (MQ=255)
                gACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                                       <  2:6951353/150‑1 (MQ=255)
                 aCCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                      <  1:4006448/150‑1 (MQ=255)
                 aCCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                      >  2:2107317/1‑150 (MQ=255)
                   cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGagggtgagggtg                                                                                                                                    >  1:9201236/1‑150 (MQ=255)
                    gggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                                                                                                   >  1:2470764/1‑150 (MQ=255)
                     ggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                  <  1:4207805/150‑1 (MQ=255)
                      gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                 <  1:553289/150‑1 (MQ=255)
                      gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                 <  1:6389659/150‑1 (MQ=255)
                             ctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAg                                                                                                                          >  2:1138693/1‑150 (MQ=255)
                             ctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAg                                                                                                                          >  1:4520657/1‑150 (MQ=255)
                              tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                         >  1:7946814/1‑150 (MQ=255)
                                gctgTGCTGGCAGATTGGGGGGCGAGGGTGTTGGTGCCTGCGTATGCTTTAAGTTTGTTTCGCAGGATTTATGGCTAACCGTTGGCAACTTTGTGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGAGGTTTAGGGTGTGTGTAAATggcg                                                                                                                       <  2:328536/147‑1 (MQ=255)
                                  aGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcggc                                                                                                                     >  2:8606885/1‑150 (MQ=255)
                                       tGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGa                                                                                                                >  2:6371666/1‑150 (MQ=255)
                                       tGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGa                                                                                                                >  2:3192595/1‑150 (MQ=255)
                                                 gggggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAg                                                                                                      >  1:905233/1‑150 (MQ=255)
                                                  ggggCGCCGGTGCTGGTGACAGCTAATGCTTTAATATTGTTGCGCAGGAGAAACGTCAAACCGATCGAACCTTTGCGCGCGATCGGTCCCCCCGCCCCTATGGGTTTTGGGTTAGGGTGTGGGCAAAAGGCGGCATCGAAGCCAATCAGc                                                                                                     <  2:2846681/150‑1 (MQ=255)
                                                  ggggCGAGGGGGGTGGTGACCGCGATTGCGTTATGATTGTGGCGCTGGAGATAAGGCAAACCGTTCGCAACATTGCGCGCGATCGGTCCCGTCGCCCCTGTGGGGTGCGGGTTTGGGGGCGGGGACAAGGCGGCATCGAAGCCAATCAGc                                                                                                     <  2:2923948/150‑1 (MQ=255)
                                                  ggggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGc                                                                                                     >  2:9115789/1‑150 (MQ=255)
                                                       gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCCATCAGCCCCTa                                                                                                >  2:5487574/1‑150 (MQ=255)
                                                       gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTa                                                                                                >  1:6988669/1‑150 (MQ=255)
                                                         gggtggtggtGCCCGCTATTGGTTTAAGTTTGTTGCGCTGGAGATACGGCAACCGGATCGAAACTTTGTGCGCGATGGGTCCCCTCGCCCCTGTGGGGAGTGGGTTTGGGTTTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATc                                                                                              <  2:395271/149‑1 (MQ=255)
                                                           gTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCaa                                                                                            >  1:1191743/1‑150 (MQ=255)
                                                            tgggggTGACAGAGATGGGGTTAATTTTGTTGCGCTGTATTTAAGGCAAACCGACCGCAACTTTGAGCGGGATCGGTCCCCCCGCCCCTCTGGGGTGTGGGTTGGGGGGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAAc                                                                                           <  2:7997048/146‑1 (MQ=255)
                                                              gtGGTGACAGCTACTGCTTTATGTTTGTTGCGCAGGATATAAGGCAAACCGATCGCAACTTTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGTGGGTTCGGGTGAGGTGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCg                                                                                         <  2:2708991/149‑1 (MQ=255)
                                                               tGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGc                                                                                        >  1:7340853/1‑150 (MQ=255)
                                                                    aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                   >  1:429852/1‑150 (MQ=255)
                                                                    aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                   >  2:4656676/1‑150 (MQ=255)
                                                                      aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                                 >  1:2342420/1‑150 (MQ=255)
                                                                      aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                                 >  2:5899032/1‑150 (MQ=255)
                                                                       gCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGa                                                                                >  1:4425220/1‑150 (MQ=255)
                                                                        cttctGCTTTAAGTTTGTGGCGCTGGAGATAAGGCAAACCTATCTCAAAATTGCGCGCGAGCGGCCCCCTCGCCCCTGTGGGGTGTGGGTTTGGGTGAGGTTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGaa                                                                               <  2:7087041/146‑1 (MQ=255)
                                                                        cGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGaa                                                                               >  2:5545911/1‑150 (MQ=255)
                                                                         gAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                                              >  2:5221453/1‑150 (MQ=255)
                                                                          cctGCGTTAAGATTGTTGCGCAGGAGATACGGCCAACCGATGGCAACATTGAGCGCGAGCGGACCCCCCGCCCCTCTGGGGTGTGGTTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATc                                                                             <  2:5103988/148‑1 (MQ=255)
                                                                          aaTGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATc                                                                             >  1:6168708/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  1:2976839/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  2:1394677/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  2:6861261/1‑150 (MQ=255)
                                                                                ttATGATTGGTGCGCTGGAGTTACGGAAAACCTATCGCAAATTTGCGCGCTATGGGTCCCCTCGCCCCTCGGGGGTGTGGGTTTGGGTGCGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                       <  1:8265637/150‑1 (MQ=255)
                                                                                 tAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAGAtcgg                                                                      >  1:3242777/1‑147 (MQ=255)
                                                                                   aGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGa                                                                    >  1:1015222/1‑150 (MQ=255)
                                                                                    gATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGAt                                                                   >  2:7300087/1‑150 (MQ=255)
                                                                                       ttttgCGCTGTATATAAGGAAAACCGATCGCAACTTTGTGCGCTATCGGTCCCCCCGCCCATCTGGGTAGTGGTTTAGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAg                                                                <  2:6603722/148‑1 (MQ=255)
                                                                                       tgttgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAg                                                                >  1:1431062/1‑150 (MQ=255)
                                                                                       tgttgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGAGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAg                                                                <  2:4947191/150‑1 (MQ=255)
                                                                                       tgtggCGCTGGAGATACGGCAAACCGACCGAAAAATTGTGCGCGAGCGGCCCCCTCGCCCCTCTGGGGTGTGTGTTTGGGTTTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAg                                                                <  1:3824925/150‑1 (MQ=255)
                                                                                          tgCGCTGTATATAAGGCAAACCGATCGCAACATTGAGCGCGATGGGTCCCCTCGCCCCTCTGGGGTGTGGTTTAGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCa                                                             <  2:459671/150‑1 (MQ=255)
                                                                                          tgCGCTGGATATATGGAAAACCTTTCGAAACTTTGAGCGCGTTCGGCCCCCTCGCCCCTCTGGGGTGTGGTTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCa                                                             <  1:5344138/150‑1 (MQ=255)
                                                                                            cgcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGt                                                           >  1:2567916/1‑150 (MQ=255)
                                                                                             gcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTc                                                          >  1:8953112/1‑150 (MQ=255)
                                                                                             gcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCAGTc                                                          >  2:5408253/1‑150 (MQ=255)
                                                                                                    aTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGt                                                   >  1:653061/1‑150 (MQ=255)
                                                                                                       aGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtctc                                                >  1:8771580/1‑150 (MQ=255)
                                                                                                        ggCAACCCGACCGAAACTTGGCGCGCGAGGGGGCCCCCCCCCCCTCGGGGTTGTGGGTTTGGGGGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                               <  2:6228063/150‑1 (MQ=255)
                                                                                                        ggCAAACCGATCGCAACATTGCGCGCTATCGGCCCCCTCGCCCCTCTGGGGTGTGGGTTCGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                               <  1:8112030/150‑1 (MQ=255)
                                                                                                        ggCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                               >  2:4820825/1‑150 (MQ=255)
                                                                                                        ggCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                               >  1:8643393/1‑150 (MQ=255)
                                                                                                        ggCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                               >  1:7473476/1‑150 (MQ=255)
                                                                                                        ggCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                               >  1:1285936/1‑150 (MQ=255)
                                                                                                         gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                              >  1:6836527/1‑150 (MQ=255)
                                                                                                           aaaCCTATCGAAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTCGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                            <  1:8471971/150‑1 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGCGCGCTATCGGTCCCCGCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                            <  1:5714544/150‑1 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                            >  1:2806726/1‑150 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                            >  2:8362484/1‑150 (MQ=255)
                                                                                                             aCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGc                                          >  2:8190075/1‑150 (MQ=255)
                                                                                                               cGATCGCAACATTGAGCGCGATCGGTCCCCACGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTATAGCaa                                        >  1:4788331/1‑150 (MQ=255)
                                                                                                                gATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                       <  2:3085653/150‑1 (MQ=255)
                                                                                                                 agcGCAACTTTGAGCGCGCTCGGTCCCCGCGCCCCGCGGGGGAGTGGGTTCGGGTGTGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAcc                                      <  2:7413161/148‑1 (MQ=255)
                                                                                                                 aTCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAcc                                      >  1:3091719/1‑150 (MQ=255)
                                                                                                                    gCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGt                                   >  1:630321/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  >  2:2263992/1‑150 (MQ=255)
                                                                                                                          ttGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt                             >  2:219268/1‑150 (MQ=255)
                                                                                                                          ttGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt                             >  2:1561663/1‑150 (MQ=255)
                                                                                                                             aGCGCTATCGGACCCCTCGCCCCTGTGGGTAGTGGGTTAGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcc                          <  1:2095302/150‑1 (MQ=255)
                                                                                                                              gcgcGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCa                         >  2:812107/1‑150 (MQ=255)
                                                                                                                               cgcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                        >  1:7655135/1‑150 (MQ=255)
                                                                                                                               cgcgATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                        >  2:5756101/1‑150 (MQ=255)
                                                                                                                                   cgcGGCCCCCGCCCCCCTCGGGGGTGTGGTTTGGGGGGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                    <  2:348870/148‑1 (MQ=255)
                                                                                                                                   aTCGGTCCCCGCGCCCCTGTGGGGCGTGGGTTAGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                    <  1:175335/150‑1 (MQ=255)
                                                                                                                                       gTCCCCTCGCCCCTGTGGGGTGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTAc                <  2:6746079/150‑1 (MQ=255)
                                                                                                                                       gTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTAc                >  2:4419184/1‑150 (MQ=255)
                                                                                                                                          cccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaa             >  2:418393/1‑150 (MQ=255)
                                                                                                                                               gCCCCTGTGGGGTGTGGGTTAGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        <  2:1913737/150‑1 (MQ=255)
                                                                                                                                               gCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        >  2:7774357/1‑150 (MQ=255)
                                                                                                                                               gCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        >  1:6034829/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat   >  1:3436258/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat   >  1:1599132/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATA  >  NC_000913/3608546‑3608844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: