BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,728,120	T→G	5.8%	T418P (ACG→CCG)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,120	0	T	G	5.8%	2642.2 / 38.4	886	T418P (ACG→CCG)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base T (280/533); new base G (70/2); total (350/536)
Fisher's exact test for biased strand distribution p-value = 7.03e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 900 total aligned reads displayed.

TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  NC_000913/3727971‑3728269

tGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGt                                                                                                                                                       >  1:4419798/1‑150 (MQ=255)

  gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGtcc                                                                                                                                                     >  2:1963121/1‑148 (MQ=255)

   cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACg                                                                                                                                                    <  1:8304485/150‑1 (MQ=255)

   cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACg                                                                                                                                                    <  2:5621929/150‑1 (MQ=255)

   cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACg                                                                                                                                                    <  2:3348361/150‑1 (MQ=255)

    tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACgg                                                                                                                                                   <  2:4459470/150‑1 (MQ=255)

       tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGtgg                                                                                                                                                >  2:2812452/1‑148 (MQ=255)

         aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTCCCCCCCCGTGCGGtgggg                                                                                                                                              >  1:5614708/1‑146 (MQ=255)

           cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGa                                                                                                                                            <  1:3447418/150‑1 (MQ=255)

            ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAg                                                                                                                                           <  1:9203255/150‑1 (MQ=255)

             gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTGAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                          <  2:4945697/150‑1 (MQ=255)

             gCATAAAGATGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTTCGGCAAAAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCGCCCGTACGGTAAtggggc                                                                                                                                          >  2:4222891/1‑145 (MQ=255)

                tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATACAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgc                                                                                                                                       <  2:6734650/150‑1 (MQ=255)

                 aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                      <  2:2977979/150‑1 (MQ=255)

                   cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTACGGTAATCGCGctgctgg                                                                                                                                    >  2:8158755/1‑149 (MQ=255)

                    gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGAc                                                                                                                                   <  2:79199/150‑1 (MQ=255)

                    cagtcgcATCTACCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTCCGGTACGCGAGctgctggc                                                                                                                                   >  2:4705752/5‑148 (MQ=255)

                      tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGGCCCCCCCCGTGCGGGGGGCGAGGTGCTGGCCg                                                                                                                                 >  2:3621970/1‑150 (MQ=255)

                       gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTGATCGAGCTGCTGACCGc                                                                                                                                >  2:3716681/1‑150 (MQ=255)

                       gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                                <  2:8021258/150‑1 (MQ=255)

                        cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGTCAACAATTCTATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCATCCTTGCTGCTCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                               <  1:9036342/150‑1 (MQ=255)

                        cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGTTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                               <  1:2533189/150‑1 (MQ=255)

                        cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                               <  2:4855869/150‑1 (MQ=255)

                         gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGGTCTCGTGCTGGTGACCGCTg                                                                                                                              >  2:3407150/1‑150 (MQ=255)

                              tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                                 <  1:8442323/142‑1 (MQ=255)

                               gttAGATGCGAGTGTTCTAACGGTTGTTGCGGCAACCATTCACTGAGCGATTGCTCTGGAGTCGCCGCGTTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTGGGCCCCCGTACGGGCATCGCGCTGCTGACCGCCGATATcc                                                                                                                        <  2:4032064/148‑1 (MQ=255)

                               ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                        <  1:3111181/150‑1 (MQ=255)

                                gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCg                                                                                                                       <  1:8036811/150‑1 (MQ=255)

                                gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                                                       <  2:8691617/150‑1 (MQ=255)

                                 tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTCCTGGAGCTGCTGGCCGCTGATATCCGc                                                                                                                      >  2:1481327/1‑150 (MQ=255)

                                  aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                                                     <  1:5495719/150‑1 (MQ=255)

                                   gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                    <  2:3150079/150‑1 (MQ=255)

                                      gCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGCCGCGGGCTGCGGGCCGCTGAGCTccgcccgcg                                                                                                                 >  2:8854872/1‑149 (MQ=255)

                                         aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                              <  2:5801987/150‑1 (MQ=255)

                                             ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                          <  1:5894204/150‑1 (MQ=255)

                                               cTAACGGTAGTTGCGGCAGCAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGcc                                                                                                        >  2:1267153/1‑150 (MQ=255)

                                                tAACGGTTGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                       <  2:8526656/150‑1 (MQ=255)

                                                tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCATCTGACGcct                                                                                                       >  2:2331224/1‑149 (MQ=255)

                                                 aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                                                      <  1:6938447/150‑1 (MQ=255)

                                                  aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGCGGTCGTCGGGCTGCTGGCGGCGGATGTGCGCCCGCGGCTGGCGCCAGt                                                                                                     >  1:4173611/1‑150 (MQ=255)

                                                    ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTGCGGGGCTCGTGCGGCTGAGCGCGGGGAGCCGGCCGCAGCTGGCGcccggtg                                                                                                   >  1:2011781/1‑145 (MQ=255)

                                                     gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGGGGTGCGCGGGCTGCTGGGGGCGGATCGCCGCCGGCTGCTGACGCCAGtggt                                                                                                  >  2:5476931/1‑147 (MQ=255)

                                                       aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                                                                <  1:8913642/150‑1 (MQ=255)

                                                       aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                                                                <  2:1125814/150‑1 (MQ=255)

                                                        gTTGCGGCAACAATTCAATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                               <  2:8367619/150‑1 (MQ=255)

                                                          tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                             <  2:7698874/150‑1 (MQ=255)

                                                           gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                                            <  1:226224/150‑1 (MQ=255)

                                                            cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg                                                                                           <  1:7695780/150‑1 (MQ=255)

                                                            cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                                                                                          <  2:3868711/150‑1 (MQ=255)

                                                                aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGGAGTCGAGCTGCTGACGGCTGTGGTCCGCCGGGGTGTGACGCCAGTACTCGCGACGcgcc                                                                                       >  2:74856/1‑150 (MQ=255)

                                                                aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTGAGCGAGCTGCTGACCGCTGATAGCCGCCCGCATCTGACGCCAGTTCTCGCTACGcgcc                                                                                       >  2:7088480/1‑150 (MQ=255)

                                                                   aaTTCAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGACTCGAGCTGCTGACCGCGGATATCCGCCCGCATCTGTCGCCAGTACTCACTACGcgccccg                                                                                    >  2:2431831/1‑150 (MQ=255)

                                                                     ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                  >  2:3544366/1‑150 (MQ=255)

                                                                            aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGGCGGGACTCGCGCTGCGGGCCGCTGGGATCCGCGGGCCGCTGACGGCCGTCCGCGCGGGGCGCCCCGcgcccgctc                                                                           >  1:1616594/1‑146 (MQ=255)

                                                                            aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTAGGGTCATCGAGCTGCTGAGCGCTGATATCCGCCCGCAGCTGACGCCCGGCCTCACTCCGCGCCCCGCCCCCCATc                                                                           >  2:7831877/1‑150 (MQ=255)

                                                                            aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTCCGGGCAGCGGGCTGCTGCCCGCGGCTGGCCGCCAGCATCTGACGCCGGGCCTCACTACGCGCCCCGCCCCCaagc                                                                           >  2:1028790/1‑148 (MQ=255)

                                                                              cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGGGCGGTAATCGGGCTGCTGACCGCGGAGCTCCGCCAGCCTCTGACGCCCGTACTCACGACGCGCCCCGcccccgagcag                                                                         >  2:6410843/1‑144 (MQ=255)

                                                                               gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                        <  1:1145250/150‑1 (MQ=255)

                                                                                  ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGGGCTGCTGACCGCGGATCTCCGCCGGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCGACgga                                                                     >  2:8995466/1‑148 (MQ=255)

                                                                                    ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGGCCGCTGGTCTCCGCCAGCATCTGACGCCGGTGCTCCCGACGCGCCCCGCCCCCCATCAACGTAac                                                                   >  2:2259285/1‑150 (MQ=255)

                                                                                     tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGCTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAGGCCACGTAacg                                                                  >  2:6877650/1‑149 (MQ=255)

                                                                                     tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAaca                                                                  <  1:8692693/150‑1 (MQ=255)

                                                                                          aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                                             <  1:4862959/150‑1 (MQ=255)

                                                                                            tCGCCGCGATCTGCGCCAGCCTTTCTGCGCCGAGTGCTGGCCCCACATCCCCCCCCGTACGGTTATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTTACGCGAGTACTGACTACGCGCCCCGCCCCCTATCAACGTAACACTTTGCg                                                           <  2:7352029/150‑1 (MQ=255)

                                                                                             cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGAATCGAGCTGCTGACCGCGGGTATCCGCCAGCAGCTGGCGCCCGTACTCACTGCGCGCCCCGCCCCCACTCAACGTAACACGGTGCgg                                                          >  2:6268934/1‑150 (MQ=255)

                                                                                              gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGGAATCGAGCTGCTGTCCGCTGATCTCCGCCAGCATCTGACGCCAGTGCGCACTACGCGCCCCGCCCCCCATCAACGGGCCCCTTTGCGGt                                                         >  2:2664875/1‑150 (MQ=255)

                                                                                               ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCGGTACTCACTACGCGCCCCGCCCCCACTCAACGTACCGCTTTGCgggt                                                        >  2:6053973/1‑148 (MQ=255)

                                                                                               ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGTCAGGGGGCTGCTGTCCGCTGAGATCCGCCAGCCTCTGGCGCCAGTGCTCACTACGCGCCCCGCCCCCGGTCAGCGGAACACGTTGCGGtg                                                        >  2:4549152/1‑149 (MQ=255)

                                                                                               ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                        <  2:2165229/150‑1 (MQ=255)

                                                                                                  cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                                     <  1:6780758/150‑1 (MQ=255)

                                                                                                    aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                   <  2:5909381/150‑1 (MQ=255)

                                                                                                       tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                                <  2:7224766/150‑1 (MQ=255)

                                                                                                         cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                                              <  1:4001237/150‑1 (MQ=255)

                                                                                                          gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                             <  2:1844754/150‑1 (MQ=255)

                                                                                                              gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                         <  2:2531962/150‑1 (MQ=255)

                                                                                                                cTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                       <  1:6474912/150‑1 (MQ=255)

                                                                                                                cTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                       <  1:8562802/150‑1 (MQ=255)

                                                                                                                 ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa                                      <  1:1031036/150‑1 (MQ=255)

                                                                                                                 ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGAACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAAACCACGTAACACTTTGCGGTTTAATACCGCAGGCATGcc                                      <  1:2981104/150‑2 (MQ=255)

                                                                                                                        gcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGTGCTGCTGCCCGCGGATATCCGCCAGCATCTGGCGCCCGTGCTCGCTACGCGCCCCGCCCCCAATCGACGGAACACGTTGCGGTGGAATACCGCAGGCCTGCACggcctc                               >  1:3722088/1‑145 (MQ=255)

                                                                                                                        gcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                               <  1:2742178/150‑1 (MQ=255)

                                                                                                                        gcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                               <  1:61243/150‑1 (MQ=255)

                                                                                                                        gcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                               <  1:8170985/150‑1 (MQ=255)

                                                                                                                         cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATGTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGACCCCTTTGCGGGTTAATACCGCAGGCATGCACGACATcc                              >  1:5146935/1‑150 (MQ=255)

                                                                                                                           cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                            <  1:7869247/150‑1 (MQ=255)

                                                                                                                           cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGTTATCCGCCAGCATCTGGCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTGACACTTTGCGGTTTAATTCAGCAGGCATGCACGACAGCCAt                            >  1:1834970/1‑150 (MQ=255)

                                                                                                                            aGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg                           >  2:5282441/1‑150 (MQ=255)

                                                                                                                             gTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATCTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc                          <  2:4152444/150‑1 (MQ=255)

                                                                                                                                cTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCAGACGCCAGTACGCACTACGCGCCCCGCCCCCAATCAACCTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCaa                       >  1:3314131/1‑149 (MQ=255)

                                                                                                                                    ccccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCCCGACATCCATGCCATCTGc                   >  2:6580582/1‑150 (MQ=255)

                                                                                                                                    ccccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCAGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                   <  1:1448836/150‑1 (MQ=255)

                                                                                                                                        acaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc               <  1:7077099/150‑1 (MQ=255)

                                                                                                                                             cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa          >  1:6355684/1‑150 (MQ=255)

                                                                                                                                              ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGGCAGCGCATaa         <  1:7206208/150‑1 (MQ=255)

                                                                                                                                              ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa         <  1:4681586/150‑1 (MQ=255)

                                                                                                                                               cccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc        >  2:4324227/1‑150 (MQ=255)

                                                                                                                                               cccccGGACGGGAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACGCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc        <  2:5129599/150‑1 (MQ=255)

                                                                                                                                                     tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc  <  2:1618824/150‑1 (MQ=255)

                                                                                                                                                     tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc  >  1:522321/1‑150 (MQ=255)

                                                                                                                                                     tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc  >  1:7439433/1‑150 (MQ=255)

TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  NC_000913/3727971‑3728269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`