Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,092 T→A 5.3% intergenic (+75/‑123) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0920TA 5.3% 2000.6 / 21.5 598intergenic (+75/‑123)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (271/275);  new base A (41/2);  total (320/278)
Fisher's exact test for biased strand distribution p-value = 3.49e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 633 total aligned reads displayed.

GATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCA  >  NC_000913/3780943‑3781241
                                                                                                                                                      |                                                                                                                                                     
     gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaa                                                                                                                                                  <  2:1545581/150‑1 (MQ=255)
      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                                                 <  1:4135250/150‑1 (MQ=255)
      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                                                 <  1:7582505/150‑1 (MQ=255)
       tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                                                                                <  2:620536/150‑1 (MQ=255)
       tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCAAACCCACACCCACAGGGGGAGGGGGCCCGTGGGCGCTGTATTTACAACCGCACTCAGAAAc                                                                                                                                                >  1:554621/1‑150 (MQ=255)
         gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAACCCACACCCACACGGAGAGGGGACCGTTCGGCGCTGTATGTACAACCTCACACAGAAAAGa                                                                                                                                              >  2:1386857/1‑150 (MQ=255)
            cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCAAACCCACGACCACAGGGGGGGGGGGCCGGTAGGCGCTGTGTTTAAACCCGCACACAGAAACGacac                                                                                                                                           >  1:9215479/1‑150 (MQ=255)
              ggggCTGGTTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGTAAGGGAATGCGTCTTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGATAGGGGACCGTTCGGCGCTTTTTGTACTCCCTCACTCTGAAACGAAACCg                                                                                                                                         <  2:8480714/150‑1 (MQ=255)
              ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCg                                                                                                                                         <  2:3210284/150‑1 (MQ=255)
              ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCg                                                                                                                                         <  2:6989089/150‑1 (MQ=255)
                ggCTGGGTAAAGAGTTGCCTGCGGCAGTGGCTCCCTTGGCTAATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                                                       <  1:6736291/150‑1 (MQ=255)
                ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                                                       <  2:4913933/150‑1 (MQ=255)
                ggCGGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGGAATGCGGCATTGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGTGGGGACGGTTCGGCGCGGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                                                       <  1:2142480/150‑1 (MQ=255)
                 gCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACAACCACAGGGAGAGGGGGCCCTTCGGCGCGGGATGTAAACCCACACACTGAAAAGACACCCCAc                                                                                                                                      >  1:5968248/1‑150 (MQ=255)
                   tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACAACCGCAGGGAGAGGGGGCCCGTAGGCGCGGTATGTACTACCACACACAGAAAACACACCCCAcaa                                                                                                                                    >  1:5312120/1‑148 (MQ=255)
                      gTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCAAACCCAATCCCTCAGGGGGAGGGGGCCCTTCGGCGCTGGATGTACTCCCGCACTCGGGAACGACACCCCAcacatt                                                                                                                                 >  2:5217770/1‑145 (MQ=255)
                       tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                                                <  2:9057186/150‑1 (MQ=255)
                        aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                                               <  2:6697884/150‑1 (MQ=255)
                        aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                                               <  1:4926392/150‑1 (MQ=255)
                          agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                                             <  2:4557621/150‑1 (MQ=255)
                           gagTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                                            <  1:1941453/150‑1 (MQ=255)
                            agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                                                           <  1:2487157/150‑1 (MQ=255)
                            agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                                                           <  2:3033958/150‑1 (MQ=255)
                                  cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                                                                     <  1:7096202/150‑1 (MQ=255)
                                   tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                                                                                                    <  1:1424909/150‑1 (MQ=255)
                                     cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                                                                                  <  2:3649316/150‑1 (MQ=255)
                                          cTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCg                                                                                                             <  1:279468/150‑1 (MQ=255)
                                          cTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCTAAACCTCACCCGCAGGGAGAGGGGGCCGTTCGGGGCGGGATGTACTCCCGCACGCTGAGACGACCCCGCACTCTGTTTTTCTCCCACGCCCCACCg                                                                                                             >  2:7699307/1‑150 (MQ=255)
                                           tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                            <  2:6594246/150‑1 (MQ=255)
                                            ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                           <  1:4984780/150‑1 (MQ=255)
                                            ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                           <  1:6642540/150‑1 (MQ=255)
                                              cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTTTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                         <  1:1005444/150‑1 (MQ=255)
                                              cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                         <  1:466366/150‑1 (MQ=255)
                                              cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                         <  1:3351245/150‑1 (MQ=255)
                                                cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                       <  1:6127124/150‑1 (MQ=255)
                                                cccATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                       <  1:7372997/150‑1 (MQ=255)
                                                 ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                      <  2:720426/150‑1 (MQ=255)
                                                 ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                      <  1:7770784/150‑1 (MQ=255)
                                                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                    <  1:3079237/150‑1 (MQ=255)
                                                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                    <  1:5408696/150‑1 (MQ=255)
                                                       cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgggagaa                                                                                                <  2:6058024/150‑5 (MQ=255)
                                                       cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                <  1:5131632/150‑1 (MQ=255)
                                                       cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCGAACCCACACCCCCCGGGAGAGGGGCCCCTTCGGCGCGGGATGGACGCCCCACCACAGGAACCCCACCCCACCCGATTTTTCTCCCCCCCCCCACCCGGGCGAGGGcccg                                                                                                >  2:6440651/1‑148 (MQ=255)
                                                        gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGACCggg                                                                                               <  2:2173946/150‑1 (MQ=255)
                                                            gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                           <  1:7893470/150‑1 (MQ=255)
                                                            gggAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACAACCGCAGGGGCTGTGGGACCTTCGGCGCTGTATTTACGACCACACACTGAAAAGACACCGCACTAATTTTTTGTCCCGAGCCCCGACGGGGGGAGGGGCggggggg                                                                                           >  2:7917778/1‑147 (MQ=255)
                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGTGGGGACGGTTCGGCGCTGTTTGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGTAGAGGGCCGGGGTGAg                                                                                          <  1:8788748/150‑1 (MQ=255)
                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                          <  2:1704839/150‑1 (MQ=255)
                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                          <  2:4694274/150‑1 (MQ=255)
                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCTAACCCACTACCTCAGGGAGAGGGGACCGTTCGGCGCGGGATGTAATCCCCCACACTGAAACGACACCCCACTCTTTTTTTATACCACGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                          >  2:5264118/1‑150 (MQ=255)
                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCTAAACCACAGGGGGAGGGGACCCTTCGGCGCGGTATGTACTCCCGCACTAAGAAACCAAACCGCACACATTTTTTATACCACGCCCCACCGGGGAGAGGGCCCGGGTGAg                                                                                          >  2:5408703/1‑150 (MQ=255)
                                                              gAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                         <  1:5769303/150‑1 (MQ=255)
                                                               aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                        <  2:5723600/150‑1 (MQ=255)
                                                                aTGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACAACCAAAGGGCGTGGGGAACCTTAGGCGCGGTATTTAATCCCACAAGCTGAAACCACACCCCAAACATTTTTTATACCACCCCCCACCCGGGGGAGGGGCGGGGTGAgggg                                                                                       >  2:402746/1‑150 (MQ=255)
                                                                    ggCATAGTCGTTTGCGCCCCCTCACCCTCACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTCTGTACTCCCTCACTCTCAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCTGGGTGAGGGGaaaa                                                                                   <  2:3990071/150‑1 (MQ=255)
                                                                          gTCGTTTGTGCCCCCTCACCCAAAACCTCAACCACAGGGAGAGGGGACCGTTAGGCGCTGTATTTAAAACCACACTCTGAAAAGACACCGCACAATTTTTTTATCCCACGCCCCAACCGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGc                                                                             >  2:919773/1‑150 (MQ=255)
                                                                             gTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACACTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACt                                                                          >  2:8836464/1‑150 (MQ=255)
                                                                              tttGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCCCTg                                                                         >  2:4327250/1‑150 (MQ=255)
                                                                                       ccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTCCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAAt                                                                >  2:5055270/1‑150 (MQ=255)
                                                                                         tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGCAAAGGCCGCACTGCTCACCACttt                                                              >  2:8952557/1‑150 (MQ=255)
                                                                                         tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAttt                                                              >  1:6591917/1‑150 (MQ=255)
                                                                                             ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                          >  2:1206477/1‑150 (MQ=255)
                                                                                              cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                         >  1:1313558/1‑150 (MQ=255)
                                                                                               tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                        >  2:7526762/1‑150 (MQ=255)
                                                                                                  cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                     >  1:6812058/1‑150 (MQ=255)
                                                                                                   ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                    >  1:2312033/1‑150 (MQ=255)
                                                                                                     tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                  >  2:278965/1‑150 (MQ=255)
                                                                                                      ctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc                                                 >  1:6265032/1‑150 (MQ=255)
                                                                                                         ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                              >  2:3568727/1‑150 (MQ=255)
                                                                                                          cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                             >  1:8095136/1‑150 (MQ=255)
                                                                                                               ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                        >  2:8090936/1‑150 (MQ=255)
                                                                                                               ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                        >  2:1826889/1‑150 (MQ=255)
                                                                                                                gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                       >  1:1657917/1‑150 (MQ=255)
                                                                                                                 cgcggggACCGGGCGGCGCTTTTTGAACCCCCTCACTATCACCCCCCCCCCCACTATTTTTTCCCCCCCCGCCCCTCCGGGGTGCGGGCGGGGGTGCGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa                                      <  2:3803390/147‑1 (MQ=255)
                                                                                                                 agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa                                      >  2:8691567/1‑150 (MQ=255)
                                                                                                                    ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                   >  1:4514934/1‑150 (MQ=255)
                                                                                                                      gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGTCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                  >  2:8310896/1‑150 (MQ=255)
                                                                                                                      gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                  >  2:3332495/1‑150 (MQ=255)
                                                                                                                      gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTATTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACAAAgggg                                  >  1:3874286/1‑150 (MQ=255)
                                                                                                                        gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                >  1:8194010/1‑150 (MQ=255)
                                                                                                                        gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                >  1:3620650/1‑150 (MQ=255)
                                                                                                                         aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGGACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                               >  1:7221507/1‑150 (MQ=255)
                                                                                                                         aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGcc                               >  2:1123321/1‑149 (MQ=255)
                                                                                                                           cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                             >  2:9104427/1‑150 (MQ=255)
                                                                                                                           cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACGAAGGGGGCCGt                             >  1:3180175/1‑150 (MQ=255)
                                                                                                                            gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                            >  2:246412/1‑150 (MQ=255)
                                                                                                                                ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                        >  2:8338217/1‑150 (MQ=255)
                                                                                                                                       tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                 >  1:8441628/1‑150 (MQ=255)
                                                                                                                                         tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt               >  1:4802911/1‑150 (MQ=255)
                                                                                                                                          gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa              >  1:2567006/1‑150 (MQ=255)
                                                                                                                                            aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTCAACCTCGTACt            >  2:5603112/1‑150 (MQ=255)
                                                                                                                                            aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt            >  1:6398477/1‑150 (MQ=255)
                                                                                                                                              tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt          >  1:8927878/1‑150 (MQ=255)
                                                                                                                                                ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTCAGGGGGCAGTATGCTAAACATCGTACTTTAc        >  2:4962540/1‑150 (MQ=255)
                                                                                                                                                ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc        >  2:3482851/1‑150 (MQ=255)
                                                                                                                                                ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc        >  1:2081668/1‑150 (MQ=255)
                                                                                                                                                ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc        >  2:6867962/1‑150 (MQ=255)
                                                                                                                                                 cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg       >  1:1112637/1‑150 (MQ=255)
                                                                                                                                                    aCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTCTGCTAAACAGCGTACTTTACGAAcc   >  1:1782299/1‑150 (MQ=255)
                                                                                                                                                      tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa  >  2:5870548/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCA  >  NC_000913/3780943‑3781241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: