Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,023,452 A→T 5.7% K239N (AAA→AAT tatC → TatABCE protein translocation system subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,023,4520AT 5.7% 1869.2 / 17.3 597K239N (AAA→AATtatCTatABCE protein translocation system subunit
Reads supporting (aligned to +/- strand):  ref base A (362/168);  new base T (2/49);  total (364/233)
Fisher's exact test for biased strand distribution p-value = 1.51e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 610 total aligned reads displayed.

CGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGAC  >  NC_000913/4023303‑4023601
                                                                                                                                                     |                                                                                                                                                     
cGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTaaa                                                                                                                                                       <  1:6243508/150‑1 (MQ=255)
 gCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAg                                                                                                                                                      >  2:1016725/1‑150 (MQ=255)
      aaaCGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGa                                                                                                                                                 >  1:8996724/1‑150 (MQ=255)
           cccGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCg                                                                                                                                            <  2:7908238/150‑1 (MQ=255)
           cccGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCg                                                                                                                                            >  2:6453855/1‑150 (MQ=255)
            ccttATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCgg                                                                                                                                           >  1:2945757/4‑150 (MQ=255)
            ccGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCgg                                                                                                                                           <  1:3418633/150‑1 (MQ=255)
            ccGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCgg                                                                                                                                           <  2:8244748/150‑1 (MQ=255)
            ccGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCgg                                                                                                                                           >  1:6496964/1‑150 (MQ=255)
                 tgtgCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAaga                                                                                                                                      <  1:8945907/150‑1 (MQ=255)
                  gtgCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAagag                                                                                                                                     <  2:5136839/150‑1 (MQ=255)
                     cTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGaa                                                                                                                                  >  2:3596820/1‑150 (MQ=255)
                     cTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGaa                                                                                                                                  >  1:7600490/1‑150 (MQ=255)
                       ggttggtGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGaaaa                                                                                                                                <  1:9123463/150‑1 (MQ=255)
                       ggttggtGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGaaaa                                                                                                                                <  2:4908611/150‑1 (MQ=255)
                        gttggtGCTTTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAac                                                                                                                               >  1:1879548/1‑150 (MQ=255)
                           ggtGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAacgac                                                                                                                            >  1:7862886/1‑150 (MQ=255)
                             tGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAACCGACGc                                                                                                                          >  1:6058428/1‑150 (MQ=255)
                               cATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTg                                                                                                                        >  1:4447451/1‑150 (MQ=255)
                                aTTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGa                                                                                                                       >  2:1153620/1‑150 (MQ=255)
                                 ttCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGaa                                                                                                                      >  2:5354546/1‑150 (MQ=255)
                                 ttCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGaa                                                                                                                      >  2:143726/1‑150 (MQ=255)
                                 ttCGTTGTCGGGATGTTGCTGACGCCACCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGaa                                                                                                                      >  2:1898721/1‑150 (MQ=255)
                                     ttGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGAGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAg                                                                                                                  >  1:8544756/1‑150 (MQ=255)
                                        tCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGaca                                                                                                               >  1:5729147/1‑148 (MQ=255)
                                        tCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGaaa                                                                                                               >  1:867570/1‑150 (MQ=255)
                                        tCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGaaa                                                                                                               >  1:3694796/1‑150 (MQ=255)
                                          gggATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGACGAGGAAAACGACGCTGAAGCAGAAAGc                                                                                                             >  1:7123278/1‑150 (MQ=255)
                                          gggATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGc                                                                                                             >  2:2564128/1‑150 (MQ=255)
                                                ttGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGaaaaa                                                                                                       >  1:4649055/1‑150 (MQ=255)
                                                ttGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGaaaaa                                                                                                       >  1:5539974/1‑150 (MQ=255)
                                                   cTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCTGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACt                                                                                                    >  1:1029139/1‑150 (MQ=255)
                                                   cTGACGCCGCCGGATGTCTTCTCGCAAACGCTGGTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTGGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGCAAGCGACACAAcg                                                                                                    >  2:8782799/1‑149 (MQ=255)
                                                      ccgccgcGGGTTGTCTTCTCGCAAACGCTGTTGGCGATCCCGCTGTACTGTCTGTTTGAATTGGGTGTCTTCTTCTCACGCTTTTACGTTGGTAATGGGCGAAATCGGGATGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTgaa                                                                                                 <  2:7359139/149‑1 (MQ=255)
                                                      aCGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTgaa                                                                                                 >  2:6007997/1‑150 (MQ=255)
                                                       cgccgcCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTgaag                                                                                                <  2:6147856/150‑1 (MQ=255)
                                                             cgGTTGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTTTTTTAAATCGGTGTCTTCTTCTCCCGTTTTTAGGTTGGTAAGGGGCTAAATCGGGAAGTGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATaaa                                                                                          <  1:1475647/150‑1 (MQ=255)
                                                             cgGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTTTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGTAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATaaa                                                                                          <  2:7082144/150‑1 (MQ=255)
                                                              ggATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAAt                                                                                         >  2:6316492/1‑150 (MQ=255)
                                                                aTGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGTAAGAGTAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTc                                                                                       <  2:2649694/150‑1 (MQ=255)
                                                                aTGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTc                                                                                       >  2:5620783/1‑150 (MQ=255)
                                                                   tcttctCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAAc                                                                                    >  1:3251343/1‑150 (MQ=255)
                                                                     ttctCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAATTCGGTGTCTTCTTCTCACGCTTTTAGGTTGGTAATGGGCGAAATCGGGAAGTGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCg                                                                                  <  2:4090492/150‑1 (MQ=255)
                                                                     ttctCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCg                                                                                  >  1:1323729/1‑150 (MQ=255)
                                                                     ttctCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCg                                                                                  >  1:5899827/1‑150 (MQ=255)
                                                                     ttctCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCg                                                                                  >  1:7279401/1‑150 (MQ=255)
                                                                     ttctCACAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCg                                                                                  >  1:3553475/1‑150 (MQ=255)
                                                                        tcGCAAAGGCTGTTGGCGATCCCGATTTACTGTCTTTTTGAAATGGGTTTCTTCTTCTTACGGTTTTACTTTGGTAATGGGCGAAATCGGTAATTGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGccc                                                                               <  2:3198199/150‑1 (MQ=255)
                                                                        tcGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGccc                                                                               >  1:2112077/1‑150 (MQ=255)
                                                                              aCGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAgg                                                                         <  2:304796/150‑1 (MQ=255)
                                                                               cGCTTTTGGCTATCCCGATGTACTGTCTTTTTTAAATCGGTTTCTTCTTCTCACGCTTTTACGTTGTTAAAGGGCTAAATCGGGAATAGTAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAggg                                                                        <  1:5204541/150‑1 (MQ=255)
                                                                               cGCTGTGGGCGATCCCGTTGTACTGTCTGTTTTAATTGGGTTTCTTCTTCTCACGATTTTACTTTGTTAATGGGCTAACTCGGGATGTGTAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAggg                                                                        <  1:3021271/150‑1 (MQ=255)
                                                                                 cTGTTGGCGATCCCGATGTACTGACTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGTAATAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCg                                                                      <  2:4780300/150‑1 (MQ=255)
                                                                                  tGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCgg                                                                     >  1:4316720/1‑150 (MQ=255)
                                                                                    ggggCGCTCCCGCTGTACTGTCTTTTTTAAATCGGGGTATTCTTCTCACGGTTTTCCGTTGGTAAAGGGCGAAATCGGTAAGATGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGtt                                                                   <  2:7743403/148‑1 (MQ=255)
                                                                                      ggCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGt                                                                 >  1:5050924/1‑150 (MQ=255)
                                                                                        cGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCa                                                               <  2:5390490/150‑1 (MQ=255)
                                                                                         gATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCat                                                              >  2:2029557/1‑150 (MQ=255)
                                                                                          aTCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCata                                                             >  1:7478470/1‑150 (MQ=255)
                                                                                          aTCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCata                                                             >  2:3419849/1‑150 (MQ=255)
                                                                                            cccGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCTGACGGGCGGTTTTccagtg                                                           >  1:8272294/1‑145 (MQ=255)
                                                                                            cccGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATg                                                           >  2:9099040/1‑150 (MQ=255)
                                                                                              cGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGa                                                         <  2:7986825/150‑1 (MQ=255)
                                                                                                   tACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACa                                                    <  2:7560402/150‑1 (MQ=255)
                                                                                                      tgtctgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGa                                                 >  2:8394112/1‑150 (MQ=255)
                                                                                                      tgtctgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGa                                                 >  1:3098745/1‑150 (MQ=255)
                                                                                                          tgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGtt                                             >  2:1594450/1‑150 (MQ=255)
                                                                                                           gtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGttt                                            <  2:6630172/150‑1 (MQ=255)
                                                                                                            tttGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCg                                                                                  >  1:1678691/1‑111 (MQ=255)
                                                                                                               gAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGata                                        >  2:449904/1‑150 (MQ=255)
                                                                                                                aaaTCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGatat                                       >  1:2512558/1‑150 (MQ=255)
                                                                                                                          cttcttctCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAt                             >  1:2178066/1‑150 (MQ=255)
                                                                                                                          cttcttctCACGATTTTACGTTGGTAATGGGCGAACTCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAt                             <  2:1748499/150‑1 (MQ=255)
                                                                                                                           ttcttctCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAtt                            >  1:2293572/1‑150 (MQ=255)
                                                                                                                            tcttctCACTATTTAAGGTTGTTAATGGGCGAAACCGGGATTTGTAAAACGACGCTGAAGCATAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAttt                           <  1:5617489/150‑1 (MQ=255)
                                                                                                                            tcttctCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATAGGGAGTACAGGATGTTTGATATCGGCGTTAAttt                           >  2:8087491/1‑150 (MQ=255)
                                                                                                                            tcttctCACGCTTTTACGGTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAttt                           >  2:2283637/1‑150 (MQ=255)
                                                                                                                              ttctCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGa                         <  2:725522/150‑1 (MQ=255)
                                                                                                                              ttcaCACGCTTTTACGTTGGTAACGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGa                         <  1:6756944/150‑1 (MQ=255)
                                                                                                                              tactcCCGATTTTACGTTGTTAAAGGGCTAAATCGGGAAGAGTAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGa                         <  1:8003200/148‑1 (MQ=255)
                                                                                                                               tctcACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGAc                        >  2:8462623/1‑150 (MQ=255)
                                                                                                                               tctcACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGAc                        >  2:4261955/1‑150 (MQ=255)
                                                                                                                               tctcACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGAc                        <  2:1181816/150‑1 (MQ=255)
                                                                                                                                 tcACGCTTTTAGGTTGTTAAAGGGCTAATTCGGTAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCa                      <  1:2735482/150‑1 (MQ=255)
                                                                                                                                 tcACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCa                      <  2:6890819/150‑1 (MQ=255)
                                                                                                                                   aCGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGt                    >  1:1574953/1‑150 (MQ=255)
                                                                                                                                   aCGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGt                    >  1:2410177/1‑150 (MQ=255)
                                                                                                                                    cGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGtt                   <  2:180964/150‑1 (MQ=255)
                                                                                                                                     gCTTTTACGTTGGTAAAGGGCTAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTc                  <  2:371885/150‑1 (MQ=255)
                                                                                                                                     gCTTTTACGTTGGTAAAGGGCGAAATCGGTAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTc                  <  2:8626024/150‑1 (MQ=255)
                                                                                                                                      cTTTTACGTTGTTAAAGGGCGAAATCGGGAAGAGTAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCg                 <  1:410467/150‑1 (MQ=255)
                                                                                                                                       ttttACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGc                >  1:8838304/1‑150 (MQ=255)
                                                                                                                                        tttACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCa               >  1:8411524/1‑150 (MQ=255)
                                                                                                                                            cGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAAttt           >  1:684654/1‑150 (MQ=255)
                                                                                                                                             gTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTg          >  1:4883836/1‑150 (MQ=255)
                                                                                                                                              ttGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGATCGCAATTTGc         >  2:2782405/1‑150 (MQ=255)
                                                                                                                                                 gTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGaa      <  1:6080810/150‑1 (MQ=255)
                                                                                                                                                 gTAAAGGCCGAAATCGGCAAGAGGAAAACGACGCAGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGaa      <  2:5038878/150‑1 (MQ=255)
                                                                                                                                                  tAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGaaa     <  1:3866309/150‑1 (MQ=255)
                                                                                                                                                  tAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGaaa     <  2:5923989/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGAC  >  NC_000913/4023303‑4023601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: