Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,048,768 A→C 6.9% K601N (AAA→AAC polA → 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,048,7680AC 6.9% 1578.4 / 25.2 529K601N (AAA→AACpolA5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
Reads supporting (aligned to +/- strand):  ref base A (303/177);  new base C (2/46);  total (305/224)
Fisher's exact test for biased strand distribution p-value = 1.43e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 545 total aligned reads displayed.

CTGCACAATCATTCTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAA  >  NC_000913/4048619‑4048917
                                                                                                                                                     |                                                                                                                                                     
      aaTCATTCTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCg                                                                                                                                                 <  2:4042160/150‑1 (MQ=255)
         cATTCTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCCGAAGAAAACGCCGGGt                                                                                                                                              >  2:3482635/1‑150 (MQ=255)
            tCTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGgc                                                                                                                                           <  2:4454958/150‑1 (MQ=255)
              tGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGgcgc                                                                                                                                         <  2:3882749/150‑1 (MQ=255)
                 agagCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGcc                                                                                                                                      <  2:1313483/150‑1 (MQ=255)
                 agagCTCACCCGTCGTCGGGCTGAGCTGGAAAAGACAGCGCATGACCTTGCAGGTGAGGAATTTACCCTTTCTTCCACCACGCAGTTACAACCCATTCTCTTTGAAAAACAGGGCATTAACCCGCTGAAGAACACGCCGGGTGGCGCGcc                                                                                                                                      <  2:7495771/150‑1 (MQ=255)
                    gCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTc                                                                                                                                   >  2:6577552/1‑150 (MQ=255)
                      tCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCaa                                                                                                                                 >  1:4034381/1‑150 (MQ=255)
                       cACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACGAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCacc                                                                                                                                >  1:5905875/1‑148 (MQ=255)
                        aCCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACg                                                                                                                               >  2:2010966/1‑150 (MQ=255)
                         cccTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTGTGAAAAACAGGGCCTTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGt                                                                                                                              >  2:5255717/1‑150 (MQ=255)
                           cTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCg                                                                                                                            >  1:3506882/1‑150 (MQ=255)
                            ttCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCgg                                                                                                                           >  2:461316/1‑150 (MQ=255)
                            ttCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGCGGCGCGCCGTCAACGTCgg                                                                                                                           >  2:7650751/1‑150 (MQ=255)
                                tgtGGCTGTGCTGGAAAAGAAAGCGCATGAAATGGCTGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTTAAAAACAGGGAATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAaga                                                                                                                       <  2:783290/148‑1 (MQ=255)
                                tCTGGCTGCGGTGGAATAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCTGTTACAAACCATTCTCTTTGTAAAACAGGGCATTAAACCGGTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAaga                                                                                                                       <  2:8058942/150‑1 (MQ=255)
                                tCTGGCTGAGCTGGAAAAGAAAGCGCATGAGATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAaga                                                                                                                       >  2:6218962/1‑150 (MQ=255)
                                tCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGTAATTTAACCTTTCTTCCACCAAGATGTTACAAACCATTATATTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAaga                                                                                                                       <  2:3178472/150‑1 (MQ=255)
                                tCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAaga                                                                                                                       >  2:5096521/1‑150 (MQ=255)
                                tCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTGTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAcga                                                                                                                       >  2:8701548/1‑150 (MQ=255)
                                   ggCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGt                                                                                                                    >  1:3619411/1‑150 (MQ=255)
                                    gCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCACCGTCGGACGAGGtc                                                                                                                   >  1:769066/1‑149 (MQ=255)
                                          cTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGTAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaa                                                                                                             <  2:1045797/150‑1 (MQ=255)
                                            ggAAAAGAAAGCGCATGAACTTGCAGGTGAGGAATTTAACCTTTCTTCCACCACGCCGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaaga                                                                                                           <  2:8257869/150‑1 (MQ=255)
                                            ggAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaaga                                                                                                           >  2:2470750/1‑150 (MQ=255)
                                            ggAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaaga                                                                                                           >  1:7415539/1‑150 (MQ=255)
                                             gAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaagaa                                                                                                          >  1:8531328/1‑150 (MQ=255)
                                             gAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaagaa                                                                                                          >  2:5163951/1‑150 (MQ=255)
                                             gAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaagaa                                                                                                          >  1:2749586/1‑150 (MQ=255)
                                             gAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaagaa                                                                                                          >  1:1861472/1‑150 (MQ=255)
                                               aaagaaagCGCGTGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACt                                                                                                        >  1:4722923/1‑150 (MQ=255)
                                                 agaaagCGCTTTATTTTGCAGTTGAGTAATTTAAAATTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGTAATgg                                                                                                      <  2:4371727/150‑1 (MQ=255)
                                                 agaaagCGCATGAAATTGCTGGTTAGTATTTTACCGTTTCTTCCACAATGCTGTTAAAAACCTTACTCTTTCAAAAACAGGGCATAAACCCGCTTAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTgg                                                                                                      <  1:5498644/150‑1 (MQ=255)
                                                 agaaagCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTgg                                                                                                      >  2:7315721/1‑150 (MQ=255)
                                                  gaaagCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGgc                                                                                                     >  1:3785824/1‑150 (MQ=255)
                                                    aagCGCATGAAATTGCTGGTGTGGAATTTAACCTTTCTTCCACCACGCAGTTAAAAACAATTCTCTTTTAAAAACAGGGCATCAACCCGCTGAATAACACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGgcgc                                                                                                   <  1:7494757/150‑1 (MQ=255)
                                                     agCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAATCAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGATGAGGTACTGGAAGTACTGGCGCt                                                                                                  >  1:703671/1‑150 (MQ=255)
                                                      gcgcATGAAAGTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTg                                                                                                 <  2:6006068/150‑1 (MQ=255)
                                                        gcATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGa                                                                                               >  1:3140427/1‑150 (MQ=255)
                                                          aTGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACt                                                                                             >  1:1368942/1‑150 (MQ=255)
                                                          aTGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACt                                                                                             >  1:4589366/1‑150 (MQ=255)
                                                              atttGCTGGTGAGGAATTTAACCTTTATTCCACCAAGCTGTTACAAACCATTCTCTTTGAAAAACAGGGCATAAACCCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATcc                                                                                         <  1:2195209/148‑1 (MQ=255)
                                                                 tggctgtgtGGATTTTAAATTTTATTCCACCAAGCATTAAAAAACCATTCTTTTTTAAAAACTGGGCATTAAACCGCTTAAGCAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGtt                                                                                      <  1:1754622/145‑1 (MQ=255)
                                                                      gtgtgtaaTTTAACTTTTATTCCACCAAGCCTTTACAAACCATTCTATTTTAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCaa                                                                                 <  1:6939132/144‑1 (MQ=255)
                                                                        gAGGAATTTAACCTTTCTTCCACCACGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCaaaa                                                                               <  1:4926285/150‑1 (MQ=255)
                                                                        gAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCaaaa                                                                               <  2:4865066/150‑1 (MQ=255)
                                                                         aGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAg                                                                              >  1:3862709/1‑150 (MQ=255)
                                                                          ggAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGt                                                                             >  1:3269186/1‑150 (MQ=255)
                                                                            aaTTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACCGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGa                                                                           <  2:8875021/150‑1 (MQ=255)
                                                                            aaTTTAACCTTTCTTCCACCAAGCAGTTACAAACAATTCTCTTTGAAAAACAGGGCATTAAACCGCTTAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGa                                                                           <  2:7259567/150‑1 (MQ=255)
                                                                              tttAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGAtt                                                                         >  2:6862373/1‑150 (MQ=255)
                                                                                tAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCt                                                                       <  2:6173010/150‑1 (MQ=255)
                                                                                 aaCCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACTGGGCATTAACCCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTg                                                                      <  1:2923479/150‑1 (MQ=255)
                                                                                 aaCCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTg                                                                      <  1:7567915/150‑1 (MQ=255)
                                                                                  aCCTTTCTTCCACCACGCTGTTACAACCCATTCTCTTTTAAAAACAGGGCATTAACCCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTgg                                                                     <  1:1952411/150‑1 (MQ=255)
                                                                                   ccTTTCTTCCAACAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATAAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGa                                                                    <  1:7825783/150‑1 (MQ=255)
                                                                                    cTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAg                                                                   >  1:6248006/1‑150 (MQ=255)
                                                                                     tttCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGt                                                                  >  2:5527762/1‑150 (MQ=255)
                                                                                     tttCTTCCACCAAGCAGTTACAAACCATTCTCTTGGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGt                                                                  >  1:2116036/1‑150 (MQ=255)
                                                                                        cttcCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATc                                                               <  1:6396544/150‑1 (MQ=255)
                                                                                        cttcCACCAAGCAGTTACAAACCATTCTATTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATc                                                               <  1:9017977/150‑1 (MQ=255)
                                                                                         ttcCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCg                                                              >  2:26664/1‑150 (MQ=255)
                                                                                         ttcCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCg                                                              >  2:3749371/1‑150 (MQ=255)
                                                                                            caccaAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTgg                                                           >  2:2741444/1‑150 (MQ=255)
                                                                                             accaAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCACCGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGGTGCCACAAGTGATTCTGGAGGATCGTggg                                                          >  2:2328276/1‑149 (MQ=255)
                                                                                             accaAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGt                                                          >  1:8088635/1‑150 (MQ=255)
                                                                                              ccaAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTc                                                         >  2:9122112/1‑150 (MQ=255)
                                                                                                  gCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGc                                                     <  2:9218170/150‑1 (MQ=255)
                                                                                                     gTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGaa                                                  >  2:3065865/1‑150 (MQ=255)
                                                                                                      ttACAAACCATTCTCTTTGAAAAACAGGGCATTAACCCGCTGAAGAACACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAg                                                 <  1:2292540/150‑1 (MQ=255)
                                                                                                      ttAAAAACCATTCTCTTTTAAAAACCGGGCATTAACCCGCTTAAGAACACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAg                                                 <  1:8696194/150‑1 (MQ=255)
                                                                                                        aCAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCt                                               <  1:4282160/150‑1 (MQ=255)
                                                                                                          aaaCCATTCTCTTTGAAAAAGAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGa                                             <  1:4811968/150‑1 (MQ=255)
                                                                                                          aaaCCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGa                                             >  1:5379656/1‑150 (MQ=255)
                                                                                                          aaaCCATTATATTTGAAAAAAAGGGCAATAAAACCCAGAAGAAAACCCCCGGTGGCGCGCCCTCAAAGTCGGAAGATGTACAGGAAGAACTGGCGCGGGACTATACGGTTCCAAAAGTGATTCTGGAGTATCGTGGTCTTGCCAAGCAGa                                             >  2:7946849/1‑150 (MQ=255)
                                                                                                            aCCATTCTCTTTGAAAAACCGGGCATTACACCGCTGAAGAACACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGaaa                                           <  1:7228807/150‑1 (MQ=255)
                                                                                                                ttCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGa                                       <  1:9156917/150‑1 (MQ=255)
                                                                                                                 tctcTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGAc                                      >  1:5169107/1‑150 (MQ=255)
                                                                                                                 tctcTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGAc                                      <  2:2162424/150‑1 (MQ=255)
                                                                                                                      tttaaaaaCAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTaca                                 <  1:6623420/147‑1 (MQ=255)
                                                                                                                       tGAAAAACAGGGCATTAACCCGCTGAAGAACACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGCTGCCAAAAGTGATTCTGGAGTTTATTTTTCTGGCGTAGCTGAAATCGACCTacac                                <  2:3342160/150‑1 (MQ=255)
                                                                                                                       tGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTacac                                >  1:3412252/1‑150 (MQ=255)
                                                                                                                          aaaaCAGGGCATTAACCCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGACGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCTTGGTCTGTCGAAGCTGAAATCGACCTACACCGa                             <  2:1157708/150‑1 (MQ=255)
                                                                                                                            aaCAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACa                           >  2:7005974/1‑150 (MQ=255)
                                                                                                                                 ggCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTg                      >  1:8001305/1‑150 (MQ=255)
                                                                                                                                  gCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGc                     >  2:6445397/1‑150 (MQ=255)
                                                                                                                                  gCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGc                     <  1:8831836/150‑1 (MQ=255)
                                                                                                                                     ttAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGc                  >  1:679140/1‑150 (MQ=255)
                                                                                                                                       aaaCCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCtg                >  2:2921483/1‑150 (MQ=255)
                                                                                                                                        aaCCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGc                                                     >  1:7880366/1‑112 (MQ=255)
                                                                                                                                        aaCCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCtga               <  1:5652573/150‑1 (MQ=255)
                                                                                                                                        aaCCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCtga               >  1:7972228/1‑150 (MQ=255)
                                                                                                                                        aaCCGCTGAAGAAAACGCCGGGTGGCGCGCCGACAACGTCGGAAGAGGTACTGGAACAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCtga               <  1:6037737/150‑1 (MQ=255)
                                                                                                                                           cGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCtgatga            >  1:1063397/1‑150 (MQ=255)
                                                                                                                                            gCTGAAGAACACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTgatgat           <  1:4399889/150‑1 (MQ=255)
                                                                                                                                             cTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATc          >  2:1413604/1‑150 (MQ=255)
                                                                                                                                              tGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCa         >  1:1526960/1‑150 (MQ=255)
                                                                                                                                                 agaaAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAAcc      >  2:177952/1‑150 (MQ=255)
                                                                                                                                                  gaaAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAAccc     <  2:1642027/150‑1 (MQ=255)
                                                                                                                                                     aaCGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGaa  <  2:8305943/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGCACAATCATTCTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAA  >  NC_000913/4048619‑4048917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: