Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,005 A→C 8.7% intergenic (‑124/+64) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0050AC 8.7% 914.1 / 19.1 326intergenic (‑124/+64)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (247/42);  new base C (2/34);  total (249/77)
Fisher's exact test for biased strand distribution p-value = 1.47e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 369 total aligned reads displayed.

TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA  >  NC_000913/379856‑380154
                                                                                                                                                      |                                                                                                                                                       
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACaaa                                                                                                                                                         <  2:1985167/150‑1 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAag                                                                                                                                                        >  2:9103200/1‑150 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAag                                                                                                                                                        >  2:3744012/1‑150 (MQ=255)
  cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAaga                                                                                                                                                       <  2:2399815/150‑1 (MQ=255)
  cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAaga                                                                                                                                                       <  1:4392770/150‑1 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAagag                                                                                                                                                      >  1:3709940/1‑150 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTAACCGGTGAATGT‑GGTCGGAAAACAAagag                                                                                                                                                      >  2:8971575/1‑150 (MQ=255)
    tctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAgg                                                                                                                                                     >  1:2633879/1‑150 (MQ=255)
    tctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACAGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAgg                                                                                                                                                     >  1:2157619/1‑150 (MQ=255)
     ctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGATAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGa                                                                                                                                                    <  2:2900297/150‑1 (MQ=255)
      tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGa‑a                                                                                                                                                  >  1:4235959/1‑150 (MQ=255)
        ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACACAGAGGA‑AA‑g                                                                                                                                               >  1:5194579/1‑150 (MQ=255)
        ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGGATATAACACTGGAGAATAAAATATATCCGGTGAATGT‑GGTCGGAACACAAAGAGGA‑AA‑g                                                                                                                                               >  2:5758113/1‑150 (MQ=255)
             gAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCATATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑gggggg                                                                                                                                          <  1:1471886/150‑1 (MQ=255)
                    gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTa                                                                                                                                   >  1:6270506/1‑150 (MQ=255)
                      gCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAGTGT‑GGTCGGAAAACTAAGAGGACAT‑GGGGGGGGGGCTaa                                                                                                                                  >  2:6968197/1‑150 (MQ=255)
                       gttttCGCACTTTATCTTCTGCTTGATATATAAGATTTGGGGGTTTTCTATATGTCAATTAATACCCCGGTTTATTTTATTAGACTGGAGAATAATATTTTTCCGGGGAATGT‑GGTCGGAATACAATGAGGA‑AA‑GGGGGGGGGGCTAATc                                                                                                                                <  2:7504724/148‑1 (MQ=255)
                       cATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATc                                                                                                                                >  1:7014852/1‑150 (MQ=255)
                         tttCGCACCTCATCATCTGCATGCAATATACTATTGGGGGTTATTCTATATTTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GTTCGTAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCgg                                                                                                                              <  2:1293778/150‑1 (MQ=255)
                          ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGc                                                                                                                             >  1:4808687/1‑150 (MQ=255)
                            cGCACCTCCTCATCTGCCTGCAATATACGATAGGGGGGTATTCTATCGGTCAATGCAGACCCCCCTACCGTATATACCCCGGGAGCATAACATTGAGCCGGTGCAGGG‑GGTCGGAAAACCCCGCGGA‑AA‑GGGGGGGGGGCTAATCGGCAg                                                                                                                           <  2:8388628/150‑1 (MQ=255)
                            cGCACCTCATCATCTGCATGCAATATACTATCGGGGGGTATTCTATATTTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAAATTATCCGGTGAATGT‑GGTCGGAAAACAACGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAg                                                                                                                           <  2:846268/150‑1 (MQ=255)
                             gCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAgg                                                                                                                          >  1:1816051/1‑150 (MQ=255)
                              cACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTCTATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAAGCGGCAggg                                                                                                                         >  2:6066308/1‑150 (MQ=255)
                               aacTCATCATATGCATGCAATATACTATAGGGGGGTATTCTAAATGTCAATGCAAACCCCCCTATAGTAAATAACACTGGAGAATAAAATTTATCCGGTGAATGA‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTCATCGGCAGGGa                                                                                                                        >  2:5004396/3‑150 (MQ=255)
                               aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGAATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGa                                                                                                                        >  2:8521486/1‑150 (MQ=255)
                                 cTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTCTATAACACTGGAGAATAAAATTTATCCGGTGACTGT‑GGGCGGAAAACAAAGAGGC‑AC‑GGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                      >  2:8687332/1‑150 (MQ=255)
                                 cTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                      >  1:7733375/1‑150 (MQ=255)
                                 cTCATCATCTGAACGCAATATACTCTCGGGGGGTATTCTATATGTCAATGAATCCCCCCCTAGTGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGGCGGAAAACAACGACGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                      <  2:7171523/150‑1 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                     >  1:4726615/1‑150 (MQ=255)
                                    atcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                                                                   >  2:2761575/1‑150 (MQ=255)
                                     tcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGGAGGGAAGgccg                                                                                                                  >  1:509548/1‑150 (MQ=255)
                                     acaccTGCATGCAATATACGATAGGGGGGTATTCTATCTGTCAATGCATCCCCCCCTATAGTATATAACACTGGAGACTAAAATTTATCCGGTGACTGT‑GGTCGGACAACCCACAGGA‑CA‑GGGGGGGGGGCTAATCGGCAGGGAAGgccg                                                                                                                  <  2:677087/146‑1 (MQ=255)
                                           gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                                            >  1:2306060/1‑150 (MQ=255)
                                           gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                                            >  1:9218587/1‑150 (MQ=255)
                                            catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                           >  2:8168107/1‑150 (MQ=255)
                                             atgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGACCACAAAGAGGA‑AC‑GGGGGGGGGGCGAATCGGCCGGGAAGGCCGCCCCGGAt                                                                                                          >  2:4964761/1‑150 (MQ=255)
                                                caATATACCATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACGGGAGAATAAAATCTATCCGGTGAACCC‑GGCCGGACACCCAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                                                                       <  2:3530118/150‑1 (MQ=255)
                                                      aCTATCGGGGTGTATTCAACATGTAACTGCATACCCCCCTAGAGTATATACCACTGGAGAATAACCTGGAGCCGGGGCCTGG‑GGGCGGCACCCCCCGAGCA‑AA‑GGGGGGGGGGCGAAGCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                                                 <  2:6633409/150‑1 (MQ=255)
                                                             gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                                          >  1:400103/1‑150 (MQ=255)
                                                             gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                                          >  2:1252799/1‑150 (MQ=255)
                                                             gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCGAATAGGCCGGGGATGCCGCCCCGGCTAGCCGGCGGCAGAAgg                                                                                          >  1:6536/1‑150 (MQ=255)
                                                               gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                                                                        >  1:3060143/1‑150 (MQ=255)
                                                               gggTACTCTATAGGTCAAGGCATACCCCCCTATAGCATATAACACGGGAGACTCCCCTTCCGCCGGCGCCCGC‑GGCCGGCCCCCCCCGCGGA‑CC‑GGGGGGGGGGCTAAGCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGattgat                                                                                        <  2:7598024/150‑6 (MQ=255)
                                                                       tttatGTCACTGCATCCCCCCCCATAGTATAGAACCCGGCCGAATAAAATTGATCCGGGTAATGG‑GGGCGGAACACACCCCGGA‑CC‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                                <  2:4336968/148‑1 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                                >  1:2745305/1‑150 (MQ=255)
                                                                          atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                                                             >  2:6468653/1‑150 (MQ=255)
                                                                            gccAAGGCATACCCCCATATAGTATACACCCCTGGCTAATAAAATTTAGCCGGTGACTGG‑GGCCGGAAAACAACGAGGA‑CA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCACCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                           <  2:5460100/148‑1 (MQ=255)
                                                                            gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                           >  1:8070905/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                          >  1:8008438/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                          >  1:5908390/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAATAGGA‑AA‑GGGGGGGGGGCTTATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCTGAAGGAATCAGAATTTCCTGGTc                                                                        >  2:4668594/1‑150 (MQ=255)
                                                                                  gCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCCGATAGCGGGCGGCAGAAGGAATCAGAAATTACAGGTCAg                                                                      >  2:4522386/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCt                                                               >  2:6128962/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                               >  2:4006526/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                               >  2:6423871/1‑150 (MQ=255)
                                                                                         ccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg                                                              >  1:1086097/1‑150 (MQ=255)
                                                                                           ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                                                           <  1:8521964/150‑1 (MQ=255)
                                                                                             tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                                                           >  1:6165408/1‑150 (MQ=255)
                                                                                                 gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                                                      >  1:6467380/1‑150 (MQ=255)
                                                                                                  tatataACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                                     >  1:8815412/1‑150 (MQ=255)
                                                                                                   atataACACTCTAGAATACACTTTCCCCGG‑GCACGTGGGCCGGAACACCACGCGGA‑AC‑GGGGGGGGGGCAAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg                                                    <  2:2633879/150‑1 (MQ=255)
                                                                                                    tataACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                                                   >  2:1762976/1‑150 (MQ=255)
                                                                                                      taccacTGGAGACTACACTTCATCCGGTGAATGG‑GGCCGGAAAACAACGCGGA‑CC‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGccc                                                 <  2:512743/147‑1 (MQ=255)
                                                                                                      taccacGGGCGAACAAACTTCAGCCGGTGACGGC‑GGACGGAAAACCCCGAGCA‑CC‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGccc                                                 <  2:2274957/147‑1 (MQ=255)
                                                                                                      taACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                  >  1:4129877/1‑150 (MQ=255)
                                                                                                       aaCACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg                                                >  2:3781742/1‑150 (MQ=255)
                                                                                                         cacTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGtt                                              >  1:7875309/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGTAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                           >  2:31319/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                           >  1:4968688/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                           >  1:8265803/1‑150 (MQ=255)
                                                                                                              gagaATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat                                         >  1:6563715/1‑150 (MQ=255)
                                                                                                              gagaATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat                                         >  2:6543736/1‑150 (MQ=255)
                                                                                                              gagaATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat                                         >  2:7775110/1‑150 (MQ=255)
                                                                                                                gaATAACATTTCGCCGGTGACTGT‑GGTCGGCAAACCACGACGC‑AC‑GGGGGGGGGGCCAAGCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatca                                       <  2:7420673/150‑1 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc                                     >  1:1870576/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                    >  1:1645573/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                    >  2:3271928/1‑150 (MQ=255)
                                                                                                                        tttATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgg                               >  1:6804012/1‑150 (MQ=255)
                                                                                                                            tCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGTGGCTAATCGACAGGGAAGGCCGCACCGGATAGCGGGCGGCAGAAGGAATAAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg                           >  2:5262203/1‑150 (MQ=255)
                                                                                                                            tCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg                           >  1:968179/1‑150 (MQ=255)
                                                                                                                             ccGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt                          >  1:5693962/1‑150 (MQ=255)
                                                                                                                              cGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTc                         >  1:3160969/1‑150 (MQ=255)
                                                                                                                              cGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGCGTGTc                         >  2:5506762/1‑150 (MQ=255)
                                                                                                                               ggTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                        >  2:1562696/1‑150 (MQ=255)
                                                                                                                                gTGAATGT‑GGTCGGAAAACACCCCGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGt                       <  2:2177725/150‑1 (MQ=255)
                                                                                                                                 tGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa                      >  2:8839119/1‑150 (MQ=255)
                                                                                                                                      gt‑gGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACcaca                 >  1:3796919/1‑150 (MQ=255)
                                                                                                                                      gt‑gGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACcaca                 >  1:6697036/1‑150 (MQ=255)
                                                                                                                                             ggAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc           >  1:6881638/1‑150 (MQ=255)
                                                                                                                                             ggAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc           >  1:5564845/1‑150 (MQ=255)
                                                                                                                                              gAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc          >  1:7591641/1‑150 (MQ=255)
                                                                                                                                              gAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc          >  2:3367100/1‑150 (MQ=255)
                                                                                                                                                  aCAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGcc      <  2:8070905/150‑1 (MQ=255)
                                                                                                                                                    aaaGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGt    >  2:6665781/1‑150 (MQ=255)
                                                                                                                                                    aaaGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGt    >  1:7266287/1‑150 (MQ=255)
                                                                                                                                                     aaGAGGA‑AAGGGGGGGGGGGCTAAACGGCAGGGAAGGCCCCCCCGGATAGCGGGCGGCAGAAGGAATAACAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAAACAACGGTTGGGGTGTCGTACCACACTTTACCTGCCga    >  1:284342/1‑149 (MQ=255)
                                                                                                                                                     aaGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc   >  1:3476954/1‑150 (MQ=255)
                                                                                                                                                     aaGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc   >  2:2525881/1‑150 (MQ=255)
                                                                                                                                                      agagGA‑AA‑GGGGGGGGGTCTAATCGGCAGGGAAGGCCCCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAATAACATGTCAGACGGGCAGCAAGTTGCAGACCGTTAAAATCATCGGTTCGGTAGTCGTACCACACATTACCTTCCGTCa  >  1:8848700/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GGTCGGAAAACAAAGAGGA‑AA‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA  >  NC_000913/379856‑380154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑