Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,928,424 C→T 6.9% D394N (GAT→AAT)  viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,928,4240CT 6.9% 194.5 / 4.3 58D394N (GAT→AAT) viaAstimulator of RavA ATPase activity; von Willebrand factor domain protein
Reads supporting (aligned to +/- strand):  ref base C (16/38);  new base T (2/2);  total (18/40)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATG  >  NC_000913/3928286‑3928557
                                                                                                                                          |                                                                                                                                     
gCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCg                                                                                                                                      >  2:423884/1‑140 (MQ=255)
  tGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGt                                                                                                                                    >  1:20855/1‑140 (MQ=255)
    cAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTgc                                                                                                                                  >  2:916946/1‑140 (MQ=255)
         ctTTCACTTTACTCGTCACGTCTTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCa                                                                                                                             <  2:403252/140‑1 (MQ=255)
           ttCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATTGGTGCCGCCACg                                                                                                                           >  1:992787/1‑140 (MQ=255)
           ttCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATTGGTGCCGCCACg                                                                                                                           >  1:992567/1‑140 (MQ=255)
                 ttACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAActg                                                                                                                     >  1:1220213/1‑140 (MQ=255)
                 ttACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAActg                                                                                                                     >  2:966077/1‑140 (MQ=255)
                 ttACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAActg                                                                                                                     >  2:725549/1‑140 (MQ=255)
                         cACGTCGTCTGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGACAACCACTGGCAAGATCGGTGCCGCCACGACACTGCTGGCTTa                                                                                                             >  2:907973/1‑140 (MQ=255)
                                   ggCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGAt                                                                                                   >  2:88037/1‑140 (MQ=255)
                                      aaCCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGc                                                                                                <  1:424258/140‑1 (MQ=255)
                                             gAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCTCGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCg                                                                                         <  1:1121803/140‑1 (MQ=255)
                                                cGATAAAATCAGAAATCACCACCTCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATg                                                                                      <  2:751676/140‑1 (MQ=255)
                                                   tAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCt                                                                                   >  2:482417/1‑140 (MQ=255)
                                                    aaaaTCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCtt                                                                                  <  1:29772/140‑1 (MQ=255)
                                                     aaaTCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTtg                                                                                 <  1:319804/140‑1 (MQ=255)
                                                     aaaTCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTtg                                                                                 <  1:872284/140‑1 (MQ=255)
                                                            aaaTCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCt                                                                          >  1:670697/1‑140 (MQ=255)
                                                              aTCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCATGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGa                                                                        <  1:985573/140‑1 (MQ=255)
                                                              aTCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGa                                                                        <  1:985574/140‑1 (MQ=255)
                                                                        cATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATaa                                                              <  2:692762/140‑1 (MQ=255)
                                                                        cATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATaa                                                              <  2:1011229/140‑1 (MQ=255)
                                                                                  aaaCCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGAtct                                                    <  1:275701/140‑1 (MQ=255)
                                                                                   aaCCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGAtctc                                                   >  1:97138/1‑140 (MQ=255)
                                                                                     ccATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCgg                                                 <  1:592612/140‑1 (MQ=255)
                                                                                     ccATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCgg                                                 <  1:233442/140‑1 (MQ=255)
                                                                                       aTTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTg                                               <  1:476620/140‑1 (MQ=255)
                                                                                       aTTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCACGGTg                                               <  1:240873/140‑1 (MQ=255)
                                                                                        ttCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTgg                                              >  2:992146/1‑140 (MQ=255)
                                                                                          cccTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCATGAAAGCTCATAACGGACGATATCGGTGGaa                                            <  2:485857/140‑1 (MQ=255)
                                                                                            cTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATTGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGaaaa                                          <  2:992567/140‑1 (MQ=255)
                                                                                            cTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATTGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGaaaa                                          <  2:992787/140‑1 (MQ=255)
                                                                                            cTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGaaaa                                          <  2:961275/140‑1 (MQ=255)
                                                                                            cTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGaaaa                                          <  1:786701/140‑1 (MQ=255)
                                                                                             tGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAAt                                         >  2:542385/1‑140 (MQ=255)
                                                                                               cTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGTGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAg                                       <  2:371934/140‑1 (MQ=255)
                                                                                                tttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGc                                      >  2:897070/1‑140 (MQ=255)
                                                                                                 ttGCAAGCGTTCCTTATTGGCGCGATAATATCTGGCATGATCGGTGCCTGCACGAAACTGCTGGCTTAATTATCTGATTGCTTGTTGGATGACTTTTGTGTCTGAATGCTCTTTTCGGTCGTTCTCGGTGGTAAATAGCa                                     <  2:557368/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATCATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTTATAAACGGTTTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGTTCTCGGTGGAAAATAGCa                                     <  1:870546/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  1:938529/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  2:354493/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  1:86713/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  1:128213/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  1:128628/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  1:560141/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  1:308322/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGATCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  1:225615/140‑1 (MQ=255)
                                                                                                 ttGCAAGCGGTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCa                                     <  2:20855/140‑1 (MQ=255)
                                                                                                            ccATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATagcag                          >  2:207982/1‑140 (MQ=255)
                                                                                                              aTACTGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAgcg                        <  1:432431/140‑1 (MQ=255)
                                                                                                              aTAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAgcg                        <  1:432371/140‑1 (MQ=255)
                                                                                                                       cgAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTc               >  1:538442/1‑140 (MQ=255)
                                                                                                                          aaaCAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGc            <  1:1013223/140‑1 (MQ=255)
                                                                                                                          aaaCAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGc            <  1:1013030/140‑1 (MQ=255)
                                                                                                                               acTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGagag       <  1:542385/140‑1 (MQ=255)
                                                                                                                                    cAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATg  <  1:655781/140‑1 (MQ=255)
                                                                                                                                    cAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATg  <  1:729779/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
GCTGCAGCTCTTTCACTTTACTCGTCACGTCGTCAGGCAACCGCTGAGCGATAAAATCAGAAATCACCACCGCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATGCCTTGTGGGCCTGAAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATG  >  NC_000913/3928286‑3928557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑