Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,936,090 T→A 5.4% L268Q (CTG→CAG)  rbsC → D‑ribose ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,936,0900TA 5.4% 240.1 / 3.4 74L268Q (CTG→CAG) rbsCD‑ribose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (47/23);  new base A (2/2);  total (49/25)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

TTCTCTTTGTGGTCTGCTGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGG  >  NC_000913/3935959‑3936227
                                                                                                                                   |                                                                                                                                         
ttCTCTTTGTGGTCTGCTGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCCCGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTggcgggcg                                                                                                                                   >  2:926038/1‑140 (MQ=255)
ttCTCTTTGTGGTCTGCTGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTggcgggcg                                                                                                                                   >  2:48907/1‑140 (MQ=255)
            tCTGCTGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCa                                                                                                                       <  2:761379/140‑1 (MQ=255)
            tCTGCTGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCa                                                                                                                       <  1:759579/140‑1 (MQ=255)
             ctgctgGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCag                                                                                                                      >  1:365091/1‑139 (MQ=255)
                          tGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGTCGGTACGAGTCTGGCGGGCGGAAAAGGTCTCATTGTTGGGACGTTg                                                                                                         >  2:900180/1‑140 (MQ=255)
                             ccGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCAGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATc                                                                                                      >  2:175082/1‑140 (MQ=255)
                             ccGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCAGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATc                                                                                                      >  2:175118/1‑140 (MQ=255)
                               gggATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCgg                                                                                                    <  1:1132277/140‑1 (MQ=255)
                                  aTCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTAGGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGAGCGgcgc                                                                                                 >  1:16060/1‑140 (MQ=255)
                                             ggCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTg                                                                                      <  1:697189/140‑1 (MQ=255)
                                             ggCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTg                                                                                      <  1:697109/140‑1 (MQ=255)
                                               cgcgTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGc                                                                                    <  1:247691/140‑1 (MQ=255)
                                                  gTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTc                                                                                 <  1:287507/140‑1 (MQ=255)
                                                  gTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTc                                                                                 <  1:184160/140‑1 (MQ=255)
                                                    ctctcCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCt                                                                               <  1:1145410/140‑1 (MQ=255)
                                                            cGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATg                                                                       >  2:171124/1‑140 (MQ=255)
                                                            cGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCAGTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATg                                                                       >  2:171283/1‑140 (MQ=255)
                                                            cGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCGGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTTATTCTTGGCTTCCTTAATAATg                                                                       >  2:171288/1‑140 (MQ=255)
                                                              cacaACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGa                                                                     >  1:514984/1‑140 (MQ=255)
                                                              cacaACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGa                                                                     >  1:536472/1‑140 (MQ=255)
                                                              cacaACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGa                                                                     >  1:214221/1‑140 (MQ=255)
                                                              cacaACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGa                                                                     >  1:210909/1‑140 (MQ=255)
                                                                   cccACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGaa                                                                <  1:413162/140‑1 (MQ=255)
                                                                   cccACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGaa                                                                <  2:302053/140‑1 (MQ=255)
                                                                   cccACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGaa                                                                <  1:531933/140‑1 (MQ=255)
                                                                    ccACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAAt                                                               <  1:471451/140‑1 (MQ=255)
                                                                         gcggGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGtt                                                          >  2:1031676/1‑140 (MQ=255)
                                                                         gcggGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGtt                                                          >  2:1077255/1‑140 (MQ=255)
                                                                          cggGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTa                                                         <  1:733038/140‑1 (MQ=255)
                                                                          cggGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTa                                                         <  2:957833/140‑1 (MQ=255)
                                                                          cggGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTa                                                         <  2:55013/140‑1 (MQ=255)
                                                                          cggGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCAATAATTCTTGTCTTCCTTAATAATGGATTGAATTTGTTa                                                         <  2:773137/140‑1 (MQ=255)
                                                                             ggACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGgt                                                      >  1:594679/1‑140 (MQ=255)
                                                                             ggACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGgt                                                      >  1:363161/1‑140 (MQ=255)
                                                                             ggACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGgt                                                      >  2:651385/1‑140 (MQ=255)
                                                                             ggACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGgt                                                      >  1:1158528/1‑140 (MQ=255)
                                                                             ggACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGgt                                                      >  2:165578/1‑140 (MQ=255)
                                                                              gACGGGCTATCAGCTGGATGCTATTGCTGCGGTGGGTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCAGTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAAGAAGGGATTCACTTTCTAAggag                                                     >  2:627701/1‑138 (MQ=255)
                                                                                 tGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGttt                                                  <  2:697262/140‑1 (MQ=255)
                                                                                  ggCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATACTGGATTGAATTTGTTAGGTGTTtc                                                 <  1:956355/140‑1 (MQ=255)
                                                                                  ggCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTtc                                                 >  1:1029700/1‑140 (MQ=255)
                                                                                    cTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTtcct                                               >  2:62448/1‑140 (MQ=255)
                                                                                       tGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTcctcct                                            >  1:386606/1‑140 (MQ=255)
                                                                                       agAGCTGGTTGCTATTGCTGCGGTGGTTCTGGGCGCTACGAGTCTGGCGGGCTGTAAAGGTCGCATTGTTGGGACGTTTATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTTAATTTGTTAGGTGTTTcctcct                                            <  2:258445/139‑1 (MQ=255)
                                                                                         aGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAAAGGATTGAATTTGTTAGGTGTTTCCTCCTAt                                          >  2:678786/1‑140 (MQ=255)
                                                                                             ggATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTAcc                                      >  1:199855/1‑140 (MQ=255)
                                                                                             ggATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTAcc                                      >  1:199756/1‑140 (MQ=255)
                                                                                             ggATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTAcc                                      >  1:199679/1‑140 (MQ=255)
                                                                                              gATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCa                                     >  2:675274/1‑140 (MQ=255)
                                                                                              gATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCa                                     >  2:629761/1‑140 (MQ=255)
                                                                                              gATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCa                                     >  2:629736/1‑140 (MQ=255)
                                                                                                  cTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAgatg                                 <  2:16060/140‑1 (MQ=255)
                                                                                                        ctgcGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTc                           >  2:221635/1‑140 (MQ=255)
                                                                                                        ctgcGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTc                           >  2:304120/1‑140 (MQ=255)
                                                                                                        ctgcGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTc                           >  2:362273/1‑140 (MQ=255)
                                                                                                        ctgcGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTc                           >  2:372836/1‑140 (MQ=255)
                                                                                                        ctgcGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTc                           >  2:41081/1‑140 (MQ=255)
                                                                                                             gtggtTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGc                      >  2:134587/1‑140 (MQ=255)
                                                                                                             gtggtTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGc                      >  2:1124737/1‑140 (MQ=255)
                                                                                                             gtggtTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGc                      >  2:134576/1‑140 (MQ=255)
                                                                                                             gtggtTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGc                      >  1:543135/1‑140 (MQ=255)
                                                                                                             gtggtTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGc                      >  1:876310/1‑140 (MQ=255)
                                                                                                             gtggtTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGc                      >  1:876319/1‑140 (MQ=255)
                                                                                                                      ggCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGAt             >  1:1115443/1‑140 (MQ=255)
                                                                                                                         ggTACGAGTCAGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGAtttt          <  1:175118/140‑1 (MQ=255)
                                                                                                                         ggTACGAGTCAGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTACTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCATATTACCAGATGATCGTCAAAGCGGTGGTGAtttt          <  1:175082/140‑1 (MQ=255)
                                                                                                                           tACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCTTCAAAGCGGTGGTGATTTTGc        >  2:674821/1‑140 (MQ=255)
                                                                                                                           tACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGc        >  2:977487/1‑140 (MQ=255)
                                                                                                                           tACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGc        >  2:54300/1‑140 (MQ=255)
                                                                                                                           tACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGc        >  1:1084793/1‑140 (MQ=255)
                                                                                                                              gAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTgg     <  2:1154453/140‑1 (MQ=255)
                                                                                                                                gTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCg   >  2:42818/1‑140 (MQ=255)
                                                                                                                                 tCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCgg  <  1:792861/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                         
TTCTCTTTGTGGTCTGCTGGCATCGCTGGCCGGGATCATTGAAGTGGCGCGTCTCTCCTCCGCACAACCCACGGCGGGGACTGGCTATGAGCTGGATGCTATTGCTGCGGTGGTTCTGGGCGGTACGAGTCTGGCGGGCGGAAAAGGTCGCATTGTTGGGACGTTGATCGGCGCATTAATTCTTGGCTTCCTTAATAATGGATTGAATTTGTTAGGTGTTTCCTCCTATTACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGG  >  NC_000913/3935959‑3936227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: