Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,957,410 G→T 5.6% V137V (GTC→GTA ilvY ← transcriptional activator of ilvC; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,957,4100GT 5.6% 392.9 / 5.9 107V137V (GTC→GTAilvYtranscriptional activator of ilvC; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (48/53);  new base T (3/3);  total (51/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 110 total aligned reads displayed.

CTACCGACACCTGATTACGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGC  >  NC_000913/3957271‑3957549
                                                                                                                                           |                                                                                                                                           
ctACCGACACCTGATTACGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTg                                                                                                                                             >  2:1111198/1‑140 (MQ=255)
ctACCGACACCTGATTACGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTg                                                                                                                                             >  2:242636/1‑140 (MQ=255)
 tACCGACACCTGATTACGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGa                                                                                                                                            <  1:367880/140‑1 (MQ=255)
   ccGACACCTGATTACGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGacc                                                                                                                                          <  1:628391/140‑1 (MQ=255)
        acCTGATTACGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCtt                                                                                                                                     >  1:426592/1‑140 (MQ=255)
         ccTGATTACGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCttt                                                                                                                                    >  2:560731/1‑140 (MQ=255)
                aCGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCg                                                                                                                             <  2:494144/140‑1 (MQ=255)
                 cGCACCGGGCAGGGCAGCGCTGGGTCAATCAGCACTACTGCCAGATACACCAGCATCGACAACGCCACATCGCAGGGCAAGGTAACCGCTTTACCCCCAATCGCCACATCCACTTCACCCGTGACCAGCTATTACATCGc                                                                                                                            >  2:1227413/1‑140 (MQ=255)
                 cGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGc                                                                                                                            >  1:1006228/1‑140 (MQ=255)
                 cGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGc                                                                                                                            >  1:742409/1‑140 (MQ=255)
                    aCCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATc                                                                                                                         >  2:795877/1‑140 (MQ=255)
                        ggcagggcagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGcc                                                                                                                     >  1:305546/1‑140 (MQ=255)
                          cagggcagCGCGGGGGCAATCAGCCCTACTGCCAGATTCTCCAGCATCGAATACGCCTCTGCGCCGGGCAAGGTTTCAGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGGGTCCACCTTTTCCATCGCATCTGCCGc                                                                                                                   <  1:1095658/140‑1 (MQ=255)
                          cagggcagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGc                                                                                                                   <  2:853976/140‑1 (MQ=255)
                               ctgcgcGGGGGCAATCGGCACTACTGCCAGATTCTCCAGCATCGATAACTCCACTGCGCCGGGCAAGGATTCCTGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTGTCCATCTCATCTGCCGCATCAc                                                                                                              <  2:171447/138‑1 (MQ=255)
                               cagCTCGGGGGCAATCTGCACTACTTCCTGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCATTCGCAAGTTCCGCTTCAACAGTGTCCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                              <  2:294362/140‑1 (MQ=255)
                               cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                              <  2:143422/140‑1 (MQ=255)
                               cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                              <  2:143362/140‑1 (MQ=255)
                               cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                              <  2:613310/140‑1 (MQ=255)
                               cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                              <  1:172115/140‑1 (MQ=255)
                               cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                              <  2:1168828/140‑1 (MQ=255)
                                  cgcgGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCag                                                                                                           >  1:831953/1‑140 (MQ=255)
                                  cgcgGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCag                                                                                                           >  1:156726/1‑140 (MQ=255)
                                     gggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtag                                                                                                        <  1:474283/140‑1 (MQ=255)
                                      ggggCTATGAGCACTACTGGCATAGTCTCCAGCATCTACAACGCCACTGAGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                       <  2:518712/140‑1 (MQ=255)
                                      ggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCTCCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                       <  2:797254/140‑1 (MQ=255)
                                      ggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                       <  2:561355/140‑1 (MQ=255)
                                      ggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                       <  2:561401/140‑1 (MQ=255)
                                      ggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                       <  1:185929/140‑1 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                              <  2:208668/132‑1 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                              <  2:207993/132‑1 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                              <  2:207815/132‑1 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                              >  1:208668/1‑132 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                              >  1:207993/1‑132 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                              >  1:207815/1‑132 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAgtagta                                                                                                      >  2:920165/1‑140 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAgtagta                                                                                                      >  2:797475/1‑140 (MQ=255)
                                       gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAgtagta                                                                                                      >  1:668018/1‑140 (MQ=255)
                                            aTCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGttt                                                                                                 >  1:436238/1‑140 (MQ=255)
                                                      ctGCCAGATTCTCAAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCAcc                                                                                       <  2:500705/140‑1 (MQ=255)
                                                      ctGCCAGATACACCAGCATCGAAAACGCCACAGCGCCGGGCAAGGATTCCGGTTTACCCGCAATCGCCCGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCAcc                                                                                       <  2:962750/140‑1 (MQ=255)
                                                      cggCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCAcc                                                                                       <  2:801365/138‑1 (MQ=255)
                                                       tGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCg                                                                                      >  2:804355/1‑140 (MQ=255)
                                                       tGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCg                                                                                      >  2:804395/1‑140 (MQ=255)
                                                        gCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGa                                                                                     <  2:156726/140‑1 (MQ=255)
                                                         ccAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCTAGGTTTCCGGTTTACCCGCAATCGCCAGTTCCGCTTCTCCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAAGCTCCACAGAc                                                                                    >  2:1122739/1‑140 (MQ=255)
                                                         ccAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGAc                                                                                    >  1:232332/1‑140 (MQ=255)
                                                           aGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACgg                                                                                  >  1:517803/1‑140 (MQ=255)
                                                           aGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGAACCGCTTCACCAGTGACCACCATTTCCATCGCATCTGCCGCCTCACCAGTAGTAAGGTTAATCTCCACCGACgg                                                                                  >  2:91381/1‑140 (MQ=255)
                                                              ttCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACAAGTAGTAAGTTTAATCTCCACCGACGGgtg                                                                               >  2:133430/1‑140 (MQ=255)
                                                              ttCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGCTTTACCCGCAATCGCCAGATCAGCTTCACCAGTGACCACCGTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGgtg                                                                               >  2:584033/1‑140 (MQ=255)
                                                                ctcCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTACTCTCCACCGACGGGTGtt                                                                             <  1:950083/140‑1 (MQ=255)
                                                                ctcCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGtt                                                                             <  1:452591/140‑1 (MQ=255)
                                                                ctcCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGtt                                                                             <  1:932074/140‑1 (MQ=255)
                                                                   cAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcg                                                                          >  1:156390/1‑140 (MQ=255)
                                                                   cAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcg                                                                          >  1:1187584/1‑140 (MQ=255)
                                                                   cAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcg                                                                          >  1:1186305/1‑140 (MQ=255)
                                                                      cATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcgcgc                                                                       >  1:847650/1‑140 (MQ=255)
                                                                      cATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcgcgc                                                                       >  1:848458/1‑140 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTCAGTTTAAACTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  <  1:407687/140‑1 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  >  1:66235/1‑140 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  <  1:37562/140‑1 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  >  1:988966/1‑140 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  <  2:810138/140‑1 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  <  2:32702/140‑1 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  <  2:320114/140‑1 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  >  1:66359/1‑140 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  >  2:204541/1‑140 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  >  1:66236/1‑140 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGGAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                  >  2:652371/1‑140 (MQ=255)
                                                                           aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGAAGTAAGTTTAATCTCCACCGACGGGTGTTACGCGCGGAAg                                                                  >  1:664147/1‑140 (MQ=255)
                                                                                     tGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGaa                                                        >  1:575342/1‑140 (MQ=255)
                                                                                     tGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGaa                                                        >  1:459557/1‑140 (MQ=255)
                                                                                         ccGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATcgg                                                    <  2:213131/140‑1 (MQ=255)
                                                                                                aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGa                                             <  2:868143/140‑1 (MQ=255)
                                                                                                aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGa                                             <  2:867906/140‑1 (MQ=255)
                                                                                                aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGa                                             <  2:868121/140‑1 (MQ=255)
                                                                                                 ggTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGAt                                            >  2:955497/1‑140 (MQ=255)
                                                                                                  gTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATg                                           <  2:517803/140‑1 (MQ=255)
                                                                                                     tCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCt                                        <  1:1020007/140‑1 (MQ=255)
                                                                                                     tCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCt                                        <  1:508347/140‑1 (MQ=255)
                                                                                                      ccGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTg                                       >  1:354250/1‑140 (MQ=255)
                                                                                                      ccGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTg                                       >  1:194640/1‑140 (MQ=255)
                                                                                                             aCCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAg                                >  2:1136182/1‑140 (MQ=255)
                                                                                                             aCCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAg                                >  1:606693/1‑140 (MQ=255)
                                                                                                                    aTCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCAc                         >  1:891345/1‑140 (MQ=255)
                                                                                                                        ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                     <  2:515061/140‑1 (MQ=255)
                                                                                                                        ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                     <  1:911437/140‑1 (MQ=255)
                                                                                                                        ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                     <  2:805315/140‑1 (MQ=255)
                                                                                                                           gATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCag                  <  1:639671/140‑1 (MQ=255)
                                                                                                                            aTCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCaga                 <  1:607991/140‑1 (MQ=255)
                                                                                                                              ccGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCagaag               <  2:369371/140‑1 (MQ=255)
                                                                                                                              ccGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCagaag               <  2:893455/140‑1 (MQ=255)
                                                                                                                              ccGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCagaag               <  1:1030131/140‑1 (MQ=255)
                                                                                                                                 cTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGata            >  2:754643/1‑140 (MQ=255)
                                                                                                                                      ccAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTaa       <  2:152390/140‑1 (MQ=255)
                                                                                                                                          tGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                       >  2:453840/1‑40 (MQ=255)
                                                                                                                                          tGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                       <  1:453840/40‑1 (MQ=255)
                                                                                                                                          tGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCg   <  1:217225/140‑1 (MQ=255)
                                                                                                                                           gACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGTGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGc  <  1:488085/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTACCGACACCTGATTACGCACCGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGC  >  NC_000913/3957271‑3957549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: