Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,957,432 G→T 5.1% A130E (GCA→GAA)  ilvY ← transcriptional activator of ilvC; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,957,4320GT 5.1% 430.7 / 6.6 117A130E (GCA→GAA) ilvYtranscriptional activator of ilvC; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (48/63);  new base T (3/3);  total (51/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 119 total aligned reads displayed.

CGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTG  >  NC_000913/3957293‑3957571
                                                                                                                                           |                                                                                                                                           
cGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTg                                                                                                                                             >  2:172115/1‑140 (MQ=255)
  ggcagggcagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGcc                                                                                                                                           >  1:305546/1‑140 (MQ=255)
    cagggcagCGCGGGGGCAATCAGCCCTACTGCCAGATTCTCCAGCATCGAATACGCCTCTGCGCCGGGCAAGGTTTCAGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGGGTCCACCTTTTCCATCGCATCTGCCGc                                                                                                                                         <  1:1095658/140‑1 (MQ=255)
    cagggcagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGc                                                                                                                                         <  2:853976/140‑1 (MQ=255)
         ctgcgcGGGGGCAATCGGCACTACTGCCAGATTCTCCAGCATCGATAACTCCACTGCGCCGGGCAAGGATTCCTGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTGTCCATCTCATCTGCCGCATCAc                                                                                                                                    <  2:171447/138‑1 (MQ=255)
         cagCTCGGGGGCAATCTGCACTACTTCCTGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCATTCGCAAGTTCCGCTTCAACAGTGTCCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                                                    <  2:294362/140‑1 (MQ=255)
         cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                                                    <  1:172115/140‑1 (MQ=255)
         cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                                                    <  2:143422/140‑1 (MQ=255)
         cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                                                    <  2:1168828/140‑1 (MQ=255)
         cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                                                    <  2:613301/140‑1 (MQ=255)
         cagCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCAc                                                                                                                                    <  2:613310/140‑1 (MQ=255)
            cgcgGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCag                                                                                                                                 >  1:156726/1‑140 (MQ=255)
            cgcgGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCag                                                                                                                                 >  1:831953/1‑140 (MQ=255)
               gggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtag                                                                                                                              <  1:474283/140‑1 (MQ=255)
                ggggCTATGAGCACTACTGGCATAGTCTCCAGCATCTACAACGCCACTGAGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                                             <  2:518712/140‑1 (MQ=255)
                ggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCTCCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                                             <  2:797254/140‑1 (MQ=255)
                ggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                                             <  1:185929/140‑1 (MQ=255)
                ggggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                                             <  2:561401/140‑1 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                                                    >  1:207993/1‑132 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                                                    >  1:208668/1‑132 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                                                    >  1:207815/1‑132 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                                                    <  2:207815/132‑1 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                                                    <  2:207993/132‑1 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTTACCACCTTTTCCATCGCATCTTCCGCATCAc                                                                                                                                    <  2:208668/132‑1 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAgtagta                                                                                                                            >  2:797475/1‑140 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAgtagta                                                                                                                            >  2:920165/1‑140 (MQ=255)
                 gggCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAgtagta                                                                                                                            >  1:668018/1‑140 (MQ=255)
                                ctGCCAGATTCTCAAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCAcc                                                                                                             <  2:500705/140‑1 (MQ=255)
                                ctGCCAGATACACCAGCATCGAAAACGCCACAGCGCCGGGCAAGGATTCCGGTTTACCCGCAATCGCCCGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCAcc                                                                                                             <  2:962750/140‑1 (MQ=255)
                                cggCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCAcc                                                                                                             <  2:801365/138‑1 (MQ=255)
                                 tGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCg                                                                                                            >  2:804395/1‑140 (MQ=255)
                                 tGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCg                                                                                                            >  2:804355/1‑140 (MQ=255)
                                  gCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGa                                                                                                           <  2:156726/140‑1 (MQ=255)
                                   cctgaatctcCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGGAGTTAGGTTAATCACCACCGAc                                                                                                          >  2:1122713/7‑140 (MQ=255)
                                   ccAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCTAGGTTTCCGGTTTACCCGCAATCGCCAGTTCCGCTTCTCCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAAGCTCCACAGAc                                                                                                          >  2:1122739/1‑140 (MQ=255)
                                   ccAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGAc                                                                                                          >  1:232332/1‑140 (MQ=255)
                                     aGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACgg                                                                                                        >  1:517803/1‑140 (MQ=255)
                                     aGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGAACCGCTTCACCAGTGACCACCATTTCCATCGCATCTGCCGCCTCACCAGTAGTAAGGTTAATCTCCACCGACgg                                                                                                        >  2:91381/1‑140 (MQ=255)
                                        ttCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACAAGTAGTAAGTTTAATCTCCACCGACGGgtg                                                                                                     >  2:133430/1‑140 (MQ=255)
                                        ttCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGCTTTACCCGCAATCGCCAGATCAGCTTCACCAGTGACCACCGTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGgtg                                                                                                     >  2:584033/1‑140 (MQ=255)
                                          ctcCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTACTCTCCACCGACGGGTGtt                                                                                                   <  1:950083/140‑1 (MQ=255)
                                          ctcCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGtt                                                                                                   <  1:452591/140‑1 (MQ=255)
                                             cAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcg                                                                                                >  1:1186305/1‑140 (MQ=255)
                                             cAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcg                                                                                                >  1:156390/1‑140 (MQ=255)
                                                cATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcgcgc                                                                                             >  1:848458/1‑140 (MQ=255)
                                                cATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCcgcgc                                                                                             >  1:847650/1‑140 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTCAGTTTAAACTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        <  1:407687/140‑1 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        >  1:988966/1‑140 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        <  1:378155/140‑1 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        <  1:37562/140‑1 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        >  1:66235/1‑140 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        <  2:320114/140‑1 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        <  2:32702/140‑1 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        >  1:66236/1‑140 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAg                                                                                        >  1:66359/1‑140 (MQ=255)
                                                     aaaaCGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGAAGTAAGTTTAATCTCCACCGACGGGTGTTACGCGCGGAAg                                                                                        >  1:664147/1‑140 (MQ=255)
                                                               tGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGaa                                                                              >  1:575342/1‑140 (MQ=255)
                                                               tGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGaa                                                                              >  1:459557/1‑140 (MQ=255)
                                                                   ccGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATcgg                                                                          <  2:213131/140‑1 (MQ=255)
                                                                          aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGa                                                                   <  2:868143/140‑1 (MQ=255)
                                                                          aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGa                                                                   <  2:868121/140‑1 (MQ=255)
                                                                          aGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGa                                                                   <  2:867906/140‑1 (MQ=255)
                                                                           ggTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGAt                                                                  >  2:955497/1‑140 (MQ=255)
                                                                            gTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATg                                                                 <  2:517803/140‑1 (MQ=255)
                                                                               tCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCt                                                              <  1:1020007/140‑1 (MQ=255)
                                                                                ccGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTg                                                             >  1:354250/1‑140 (MQ=255)
                                                                                ccGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTg                                                             >  1:194640/1‑140 (MQ=255)
                                                                                       aCCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAg                                                      >  2:1136182/1‑140 (MQ=255)
                                                                                       aCCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAg                                                      >  1:606693/1‑140 (MQ=255)
                                                                                              aTCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCAc                                               >  1:891345/1‑140 (MQ=255)
                                                                                                  ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                                           <  2:515061/140‑1 (MQ=255)
                                                                                                  ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                                           <  2:805315/140‑1 (MQ=255)
                                                                                                  ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                                           <  1:911437/140‑1 (MQ=255)
                                                                                                  ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGGAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAg                                           <  2:805564/140‑1 (MQ=255)
                                                                                                      aTCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCaga                                       <  1:607991/140‑1 (MQ=255)
                                                                                                        ccGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCagaag                                     <  1:1030131/140‑1 (MQ=255)
                                                                                                        ccGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCagaag                                     <  2:369597/140‑1 (MQ=255)
                                                                                                        ccGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCagaag                                     <  2:369371/140‑1 (MQ=255)
                                                                                                           cTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGata                                  >  2:754643/1‑140 (MQ=255)
                                                                                                                ccAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTaa                             <  2:152390/140‑1 (MQ=255)
                                                                                                                ccAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGGAGGAAGTTGAATCTCCACCGACGGGGGTGCCGCGCGGAAGCGATCCAGACTCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTaa                             >  1:531253/1‑140 (MQ=255)
                                                                                                                    tGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                                             >  2:453840/1‑40 (MQ=255)
                                                                                                                    tGACCACCTTTTCCATCGCATCTGCCGCATCACCagtagt                                                                                                                             <  1:453840/40‑1 (MQ=255)
                                                                                                                     gACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGTGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGc                        <  1:488085/140‑1 (MQ=255)
                                                                                                                      accaccTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGcc                       >  1:192407/1‑140 (MQ=255)
                                                                                                                          ccTTTTCCATCGCATCTGCCGCATCACCAGTAGTATGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCagag                   <  2:1013464/140‑1 (MQ=255)
                                                                                                                          ccTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCagag                   <  1:157029/140‑1 (MQ=255)
                                                                                                                          ccTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCagag                   <  2:1058319/140‑1 (MQ=255)
                                                                                                                              ttCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCg               <  1:111697/140‑1 (MQ=255)
                                                                                                                              ttCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCg               >  1:1153024/1‑140 (MQ=255)
                                                                                                                               tCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGa              <  1:955497/140‑1 (MQ=255)
                                                                                                                                 cATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACg            <  1:754643/140‑1 (MQ=255)
                                                                                                                                  aTCGCATCTGCCGCATCACCAGAAGTAAGTTAAATCTCCACCGACTGGTGTTACGCGCTGAAGCGATCCAGAATCGGCGGCATATGGCTGTAGGCAGCGGTCACCGAGCACAAGATATGTAATTAGCCTGAGAGCGACgg           <  2:947662/140‑1 (MQ=255)
                                                                                                                                     gCATCTGCCGCATCACCCGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATAGGTAATTCGCCAGAGTGCGACGGccc        >  2:219437/1‑140 (MQ=255)
                                                                                                                                     gCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGccc        >  2:680725/1‑140 (MQ=255)
                                                                                                                                        tCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTg     <  2:220382/140‑1 (MQ=255)
                                                                                                                                        tCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTg     <  2:587730/140‑1 (MQ=255)
                                                                                                                                        tCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTg     <  2:781907/140‑1 (MQ=255)
                                                                                                                                         cTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGc    >  2:849787/1‑140 (MQ=255)
                                                                                                                                           gCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTg  <  2:712813/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CGGGCAGGGCAGCGCGGGGGCAATCAGCACTACTGCCAGATTCTCCAGCATCGAAAACGCCACTGCGCCGGGCAAGGTTTCCGGTTTACCCGCAATCGCCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTG  >  NC_000913/3957293‑3957571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: