Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 377,374 T→C 5.1% intergenic (+63/+161) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913377,3740TC 5.1% 436.7 / 11.4 138intergenic (+63/+161)yaiL/frmBDUF2058 family protein/S‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base T (54/75);  new base C (2/5);  total (57/81)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 146 total aligned reads displayed.

CGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTC  >  NC_000913/377236‑377507
                                                                                                                                          |                                                                                                                                     
cgcgcTCAGCGCCGAAGATCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTCTGCGCGTCTTATCAGGCCTACGGtt                                                                                                                                      >  1:138236/1‑140 (MQ=255)
cgcgcTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGtt                                                                                                                                      >  1:549446/1‑140 (MQ=255)
cgcgcTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGtt                                                                                                                                      >  1:549407/1‑140 (MQ=255)
cgcgcTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGtt                                                                                                                                      >  1:401866/1‑140 (MQ=255)
cgcgcTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGtt                                                                                                                                      >  1:129611/1‑140 (MQ=255)
cgcgcTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGAAGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGtt                                                                                                                                      >  1:600566/1‑140 (MQ=255)
   gatgcgacgctaacgcgtcttatcaggcctacaaattcccgcgccatccgtaggccggataaggcgttcacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGCTtac                                                                                                                                   <  2:819487‑M2/26‑2 (MQ=255)
   gatgcgacgctaacgcgtcttatcaggcctacaaattcccgcgccatccgtaggccggataaggcgttcacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGCTtac                                                                                                                                   <  2:101617‑M2/26‑2 (MQ=255)
   gatgcgaagcttacgcttcttaacatgccaactacttccctcgccatacgtagtccggataaggctttcacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGCTtac                                                                                                                                   <  2:1118546‑M2/26‑2 (MQ=255)
    atgcgacgctaacgcgtcttatcaggcctacaaattcccgcgccatccgtaggccggataaggcgttcacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGTTTAt                                                                                                                                   <  2:189796‑M2/26‑1 (MQ=255)
       aGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGc                                                                                                                               <  2:97312/140‑1 (MQ=255)
        gcgcCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCg                                                                                                                              <  1:253299/140‑1 (MQ=255)
        gcgcCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCg                                                                                                                              <  1:853344/140‑1 (MQ=255)
        gcgcCGAAGAGCAGGATGAACACGACCCGTCTGCCGACTTCAAAGTGCCTGACGCTTGGATGTGGGAAACATAGATAAGGCGTGCGCAGATGCCTGATGCGACGCGATGAGCGACTTAACAGGCCTACGGTTTATGGGCg                                                                                                                              <  2:357315/140‑1 (MQ=255)
          gcCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGaa                                                                                                                            >  1:1207081/1‑140 (MQ=255)
             gAAGATCAGGCTGAACACGACCCGTCTGCCGACTTCAAAGTGCCTGATGAATAGATGTGCTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAgtg                                                                                                                         <  2:852793/140‑1 (MQ=37)
              aaGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAgtgt                                                                                                                        >  1:439967/1‑140 (MQ=255)
                 agCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGa                                                                                                                     <  2:1207081/140‑1 (MQ=255)
                 agCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGa                                                                                                                     <  1:678230/140‑1 (MQ=255)
                 agCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGa                                                                                                                     <  1:806642/140‑1 (MQ=255)
                 agCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGa                                                                                                                     <  2:1011613/140‑1 (MQ=255)
                  gCAGGAAGCCGACGACCCGTTAGCCGACATCAAAGTGACTGATGATTTGATGTGGTAAACATATATACGTCGTGCGCAGATGCCTGATGCGACGCTATGCTCGTCGTATCAGGCCTACGGTTTATGGGCGACGTGTAGAc                                                                                                                    <  2:165328/140‑1 (MQ=37)
                   cAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGAcc                                                                                                                   >  2:68893/1‑140 (MQ=255)
                     ttatcaggcctacaaattcccgcgccatccgtaggccggataaggcgtttacgccgcatccggcagtcgtgcgcagatGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCgg                                                                                                                 >  1:1015724‑M2/79‑140 (MQ=255)
                      ttatcaggcctacaaattcccgcgccatccgtaggccggataaggcgttcacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGCTtac                                                                                                                                   <  1:343336‑M2/26‑2 (MQ=255)
                      ttatcaggcctacaaattcccgcgccatccgtaggccggataaggcgttcacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGCTtac                                                                                                                                   >  2:343336‑M2/96‑120 (MQ=255)
                               gacCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGtt                                                                                                       <  2:783924/140‑1 (MQ=255)
                                acCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTc                                                                                                      >  1:1096686/1‑140 (MQ=255)
                                acCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTc                                                                                                      >  1:1096852/1‑140 (MQ=255)
                                acCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCGATGCGCGTCTTAGCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATCAGGCGTTc                                                                                                      >  2:925875/1‑140 (MQ=255)
                                   cGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAACCATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCAcg                                                                                                   <  2:945799/140‑1 (MQ=255)
                                   cGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCAcg                                                                                                   <  1:865685/140‑1 (MQ=255)
                                     tATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCg                                                                                                                              <  1:197809/111‑1 (MQ=255)
                                      tcccgcgccatccgtaggccggataaggcgtttacgccgcatccggcagtcgtgcgcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGtt                                                                                                       <  2:658178‑M2/72‑1 (MQ=255)
                                       tcccgcgccatccgtaggccggataaggcgtttacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGtt                                                                                                       >  1:691351‑M2/79‑132 (MQ=255)
                                       tcccgcgccatccgtaggccggataaggcgtttacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGtt                                                                                                       <  2:188544‑M2/54‑1 (MQ=255)
                                       tcccgcgccatccgtaggccggataaggcgtttacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGtt                                                                                                       <  2:691351‑M2/54‑1 (MQ=255)
                                       tcccgcgccatccgtaggccggataaggcgtttacgccgcatccggcagtcgtgcgcagatgcctgatgcgacgctaaCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGtt                                                                                                       >  1:188544‑M2/79‑132 (MQ=255)
                                              ttCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                        >  2:922291/1‑140 (MQ=255)
                                                   gtaggccggataaggcgtttacgccgcatccggcagtcgtgcgcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  2:258363‑M2/92‑1 (MQ=255)
                                                   aGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  2:940731/140‑1 (MQ=255)
                                                   aGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  1:1806/140‑1 (MQ=255)
                                                   aGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  1:346827/140‑1 (MQ=255)
                                                   aGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  2:891505/140‑1 (MQ=255)
                                                   aGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  1:93930/140‑1 (MQ=255)
                                                   aGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  2:285273/140‑1 (MQ=255)
                                                   aGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  2:285032/140‑1 (MQ=255)
                                                    gTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATGCGGCGTGAACGCCTTATCCGGCAGTCGt                                                                                  <  2:719853/140‑1 (MQ=25)
                                                    gTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGt                                                                                  <  1:1215969/140‑1 (MQ=255)
                                                    gTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGt                                                                                  <  2:26240/140‑1 (MQ=255)
                                                       ccTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTgcg                                                                               >  1:687854/1‑140 (MQ=255)
                                                        cTGATAATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTgcgc                                                                              >  1:27855/1‑140 (MQ=255)
                                                                        gTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACg                                                              >  2:207119/1‑140 (MQ=255)
                                                                        gTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACg                                                              >  1:200301/1‑140 (MQ=255)
                                                                        gTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACg                                                              >  1:201622/1‑140 (MQ=255)
                                                                        gTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACg                                                              >  1:201711/1‑140 (MQ=255)
                                                                             cATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTg                                                         <  2:755536/140‑1 (MQ=255)
                                                                             cATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTg                                                         <  2:179771/140‑1 (MQ=255)
                                                                                aGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTgcgc                                                      >  2:581113/1‑140 (MQ=255)
                                                                                 gATACGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCACATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGcgcg                                                     <  2:436178/140‑1 (MQ=255)
                                                                                  aTAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGt                                                    >  2:46475/1‑140 (MQ=255)
                                                                                  aTAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGt                                                    >  1:1007078/1‑140 (MQ=255)
                                                                                  aTAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGt                                                    >  1:1006974/1‑140 (MQ=255)
                                                                                       tcgtgcgcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTAt                                               >  1:387608‑M2/13‑140 (MQ=255)
                                                                                       tcgtgcgcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTAt                                               <  2:616235‑M2/128‑1 (MQ=255)
                                                                                       tcgtgcgcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTAt                                               <  2:387608‑M2/128‑1 (MQ=255)
                                                                                          tgcgcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAg                                            >  2:541539‑M2/10‑140 (MQ=255)
                                                                                          tgcgcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAg                                            >  1:25984‑M2/10‑140 (MQ=255)
                                                                                            cgcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGc                                          >  1:512751‑M2/8‑140 (MQ=255)
                                                                                             gcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGa                                                                                                                             <  2:469033‑M2/50‑1 (MQ=255)
                                                                                             gcagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGa                                                                                                                             >  1:469033‑M2/7‑56 (MQ=255)
                                                                                              cagatGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTAt                                                                                                                                   <  2:362613‑M2/44‑1 (MQ=255)
                                                                                                           CGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGc                           <  1:270310‑M2/140‑1 (MQ=255)
                                                                                                            gtCGCTATGCGCGTCTTATCATGCCTACGGGTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGGCATGCCTGCAGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCg                          <  2:305028‑M2/138‑1 (MQ=255)
                                                                                                            GACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCg                          <  2:1058944‑M2/140‑1 (MQ=255)
                                                                                                            GACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCg                          <  2:790913‑M2/140‑1 (MQ=255)
                                                                                                            GACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGGTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCg                          <  2:304465‑M2/140‑1 (MQ=255)
                                                                                                                     CGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAgg                 <  2:788646‑M2/140‑1 (MQ=255)
                                                                                                                      GCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGc                <  1:880937‑M2/140‑1 (MQ=255)
                                                                                                                      GCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGc                <  2:20696‑M2/140‑1 (MQ=255)
                                                                                                                      GCGTCTCAACAGGCCTACGGTGGATGGGCGAAGTGTAGACCGGATTAGGCGTTGAGGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGc                <  2:20669‑M2/140‑1 (MQ=255)
                                                                                                                        GTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCg              >  1:752234‑M2/1‑140 (MQ=255)
                                                                                                                          CTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGc                                                                  <  2:4824‑M2/86‑1 (MQ=255)
                                                                                                                          CTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGc                                                                  >  1:4824‑M2/1‑86 (MQ=255)
                                                                                                                          CTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGc                                                                  <  2:4876‑M2/86‑1 (MQ=255)
                                                                                                                          CTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGc                                                                  >  1:4876‑M2/1‑86 (MQ=255)
                                                                                                                          CTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGa            >  1:1216342‑M2/1‑140 (MQ=255)
                                                                                                                          CTTATCAGGCCTACGGGTAATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCGTTGCGCGTCTTATCAGGCCTACGGCGAACGGGCGAAGGGAAGGCCGGa            >  2:1039298‑M2/1‑140 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTc                                                                                    >  1:786459‑M2/1‑67 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTc                                                                                    >  1:18763‑M2/1‑67 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg                                                                                   <  1:526633‑M2/68‑1 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGt                                                                                  <  2:511885‑M2/69‑1 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTAc                               >  1:413579‑M2/1‑120 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTAc                               <  2:1143144‑M2/120‑1 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTAc                               <  2:413579‑M2/120‑1 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTAc                               >  1:1143144‑M2/1‑120 (MQ=255)
                                                                                                                           TTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGGGTAGGCCGGAt           >  1:642425‑M2/1‑140 (MQ=255)
                                                                                                                             ATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATaa         <  1:806515‑M2/140‑1 (MQ=255)
                                                                                                                                   CCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGtt   <  2:1069026‑M2/140‑1 (MQ=255)
                                                                                                                                    CTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTc  >  2:1197758‑M2/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                     
CGCGCTCAGCGCCGAAGAGCAGGATGAAGACGACCCGTATGCCGACTTCAAAGTGCCTGATGATTTGATGTGGTAAACATAGATAAGTCGTGCGCAGATGCCTGATGCGACGCTATGCGCGTCTTATCAGGCCTACGGTTTATGGGCGAAGTGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCCATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTACGGCTTACGGGCGAAGTGTAGGCCGGATAAGGCGTTC  >  NC_000913/377236‑377507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: