Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,484,773 A→G 5.6% T393T (ACT→ACC pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,484,7730AG 5.6% 238.5 / 3.8 71T393T (ACT→ACCpepAmultifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
Reads supporting (aligned to +/- strand):  ref base A (13/54);  new base G (2/2);  total (15/56)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTCGCCATATCGGCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGTCGGTGTTCAG  >  NC_000913/4484636‑4484907
                                                                                                                                         |                                                                                                                                      
ttCGCCATATCGGCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAATCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGt                                                                                                                                      <  2:636775/140‑1 (MQ=255)
ttCGCCATATCGGCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGt                                                                                                                                      <  2:543260/140‑1 (MQ=255)
  cGCCATATCGGCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTaa                                                                                                                                    <  2:272693/140‑1 (MQ=255)
  cGCCATATCGGCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTaa                                                                                                                                    <  2:272644/140‑1 (MQ=255)
  cGCCATATCGGCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTaa                                                                                                                                    <  2:272659/140‑1 (MQ=255)
     cATATCGGCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAatat                                                                                                                                 >  1:4841/1‑140 (MQ=255)
      tgctCGGCATCATTGGACTCCAGTTGGTGCTGTGCCTCGTGCCCCCGCGGTAAGCGCCAGGCGCGGTCACCGGTTTGTTCAGACGCGGCTATCAGTTCATGGGCCAGGGGATTATGGTTCGCCATCAGACCAGTCATctg                                                                                                                                <  2:1200249/137‑1 (MQ=255)
            gCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATGAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGAcc                                                                                                                          >  2:1071521/1‑140 (MQ=255)
            gCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGAcc                                                                                                                          >  2:1071550/1‑140 (MQ=255)
                 aTTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAgcg                                                                                                                     <  2:927839/140‑1 (MQ=255)
                 aTTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAgcg                                                                                                                     <  1:134317/140‑1 (MQ=255)
                 aTTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAgcg                                                                                                                     <  2:927838/140‑1 (MQ=255)
                 aTTGGACTCCAGTCGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTACAGACGCGGCAAACAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAgcg                                                                                                                     <  1:134401/140‑1 (MQ=255)
                          cAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGc                                                                                                            <  1:28616/140‑1 (MQ=255)
                          cAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGc                                                                                                            <  1:29916/140‑1 (MQ=255)
                          cAGTTGTTCCTGAAACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGATCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGCTGACCCAGCGCGATCACGc                                                                                                            <  1:29966/140‑1 (MQ=255)
                                ttCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCAc                                                                                                      >  1:749274/1‑140 (MQ=255)
                                  ccTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCg                                                                                                    >  2:690101/1‑140 (MQ=255)
                                  ccTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCg                                                                                                    >  2:540671/1‑140 (MQ=255)
                                            gTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCACTCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCg                                                                                          >  2:272557/1‑140 (MQ=255)
                                            gTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCg                                                                                          >  2:411129/1‑140 (MQ=255)
                                            gTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCg                                                                                          >  2:272556/1‑140 (MQ=255)
                                              cACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGcc                                                                                        <  1:86152/140‑1 (MQ=255)
                                              cACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGcc                                                                                        <  1:377073/140‑1 (MQ=255)
                                              cACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGcc                                                                                        <  2:754852/140‑1 (MQ=255)
                                              cACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGcc                                                                                        <  1:86428/140‑1 (MQ=255)
                                               aCCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCa                                                                                       <  2:859255/140‑1 (MQ=255)
                                                                           ccGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACg                                                           <  2:106370/140‑1 (MQ=255)
                                                                           ccGGATTGTTCAGACGCGGCAATCAGTTCAAGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACg                                                           <  2:106455/140‑1 (MQ=255)
                                                                             ggATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCt                                                         <  1:787276/140‑1 (MQ=255)
                                                                                ttGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCaa                                                      <  2:1186516/140‑1 (MQ=255)
                                                                                  gTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACg                                                    <  2:700427/140‑1 (MQ=255)
                                                                                            ggCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAaca                                          <  2:844635/140‑1 (MQ=255)
                                                                                             gCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCGGTAATGTGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAacac                                         >  1:1093054/1‑140 (MQ=255)
                                                                                             gCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCGGTAATGTGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAacac                                         >  1:1093244/1‑140 (MQ=255)
                                                                                             gCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAacac                                         >  1:528076/1‑140 (MQ=255)
                                                                                             gCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAacac                                         >  1:22437/1‑140 (MQ=255)
                                                                                              cAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGCCCAGTACTATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACg                                        <  1:239321/140‑1 (MQ=255)
                                                                                              cAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACg                                        <  1:1198920/140‑1 (MQ=255)
                                                                                              cAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACg                                        <  1:341280/140‑1 (MQ=255)
                                                                                              cAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACg                                        <  1:1198935/140‑1 (MQ=255)
                                                                                                 tCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCg                                     <  2:579793/140‑1 (MQ=255)
                                                                                                 tCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCg                                     <  1:1034614/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTACTATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:47764/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:438517/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:1153807/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:12006/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:175920/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:207030/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  2:324536/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:418466/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:438568/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:486771/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:909727/140‑1 (MQ=255)
                                                                                                   aGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGca                                   <  1:1062911/140‑1 (MQ=255)
                                                                                                             ccAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAgg                         <  2:791551/140‑1 (MQ=255)
                                                                                                              cAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAggc                        <  2:297742/140‑1 (MQ=255)
                                                                                                                  ggATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGcc                    <  2:188522/140‑1 (MQ=255)
                                                                                                                          gTTCGCCATCAGACCGGTAATGTGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGt            <  2:1093054/140‑1 (MQ=255)
                                                                                                                          gTTCGCCATCAGACCGGTAATGTGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGt            <  2:1093244/140‑1 (MQ=255)
                                                                                                                          gTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGt            <  2:964745/140‑1 (MQ=255)
                                                                                                                          gTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGt            <  1:889649/140‑1 (MQ=255)
                                                                                                                            tCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACATTACCAGGCGGCCTTCAGCGTCg          <  1:518648/140‑1 (MQ=255)
                                                                                                                            tCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGTCg          <  1:508127/140‑1 (MQ=255)
                                                                                                                            tCGCCATCAGACCAGATATATGATGACCCAGCGCGATCACGCAGGCACCGGTCCGCGTCGCCACGACAATCACCGCTTCCGGCTCAAAACGCACAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGTCg          <  2:1209315/140‑1 (MQ=255)
                                                                                                                             cGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAgg                         <  2:353243/124‑1 (MQ=255)
                                                                                                                             cGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAgg                         >  1:353243/1‑124 (MQ=255)
                                                                                                                              gCCATCAGACCAGTAATATGTTGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCATCGTAAGTTATCACGTCGCACAGTACCAGGCGGCCTTCAGCGTCGgt        >  2:27820/1‑140 (MQ=255)
                                                                                                                                 aTCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGTCGGTGtt     <  2:517930/140‑1 (MQ=255)
                                                                                                                                 aTCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGTCGGTGtt     <  2:515725/140‑1 (MQ=255)
                                                                                                                                    aGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGTCGGTGTTCAg  <  2:1059038/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TTCGCCATATCGGCAAAATTGGACTCCAGTTGTTCCTGATACTCGTCACCCAGCGGTAAGCGCCATGCGCGGTCACCGGATTGTTCAGACGCGGCAATCAGTTCATGGGCCAGCGGATTATGGTTCGCCATCAGACCAGTAATATGATGACCCAGCGCGATCACGCAGGCACCGGTCAGCGTCGCCACGTCAATCACCGCTTCCGGCTCAAAACGCTCAACGTAAGTTAACACGTCGCACAGTACCAGGCGGCCTTCAGCGTCGGTGTTCAG  >  NC_000913/4484636‑4484907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: