Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,559,943 C→A 6.9% G20C (GGC→TGC)  yjiG ← SpmB family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,559,9430CA 6.9% 213.7 / 2.9 58G20C (GGC→TGC) yjiGSpmB family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (32/22);  new base A (2/2);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTA  >  NC_000913/4559812‑4560078
                                                                                                                                   |                                                                                                                                       
tCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcg                                                                                                                                 >  2:1030354/1‑140 (MQ=255)
    cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGcc                                                                                                                             <  1:343437/140‑1 (MQ=255)
    cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGcc                                                                                                                             <  1:706856/140‑1 (MQ=255)
     acaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGTATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGCCGCGCGCCa                                                                                                                            >  2:408042/1‑140 (MQ=255)
     acaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCa                                                                                                                            >  1:378549/1‑140 (MQ=255)
         cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGa                                                                                                                        <  2:88106/140‑1 (MQ=255)
              tgaCGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAAc                                                                                                                   <  1:932967/138‑1 (MQ=255)
                   ggTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATc                                                                                                              >  2:1178406/1‑140 (MQ=255)
                       cacaGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATg                                                                                                          >  1:361275/1‑140 (MQ=255)
                        acaGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGTATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGa                                                                                                         >  1:1218963/1‑140 (MQ=255)
                        acaGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCGGCGGGTTGGTCGTGGCGAGAGTAAAGCCACGGCGCGCGCCAGCGAGAATCataggcgggg                                                                                                         >  1:1218792/1‑133 (MQ=255)
                              atGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGtttt                                                                                                   >  2:1004316/1‑140 (MQ=255)
                                gCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTg                                                                                                 <  1:420174/140‑1 (MQ=255)
                                gCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTg                                                                                                 <  1:419931/140‑1 (MQ=255)
                                 ccccACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCAACGGCGCGCGCCATCGa                                                                                                                        >  1:93557/1‑116 (MQ=255)
                                 ccccACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCAACGGCGCGCGCCATCGa                                                                                                                        >  1:93914/1‑116 (MQ=255)
                                 ccccACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCAACGGCGCGCGCCATCGa                                                                                                                        <  2:93914/116‑1 (MQ=255)
                                 ccccACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCAACGGCGCGCGCCATCGa                                                                                                                        <  2:93557/116‑1 (MQ=255)
                                  cccacccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCTGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCg                                                                                               <  1:506276/140‑1 (MQ=255)
                                    cacccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGa                                                                                             >  2:504366/1‑140 (MQ=255)
                                    cacccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGa                                                                                             >  1:1039439/1‑140 (MQ=255)
                                    cacccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGa                                                                                             >  1:1040991/1‑140 (MQ=255)
                                    cacccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGa                                                                                             >  2:731537/1‑140 (MQ=255)
                                    cacccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGGTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGa                                                                                             >  1:358853/1‑140 (MQ=255)
                                      cccaGTCGAGCAGGCCGTTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCGGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                                                                                           >  1:811925/1‑140 (MQ=255)
                                      cccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                                                                                           >  2:258824/1‑140 (MQ=255)
                                      cccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                                                                                           >  1:745317/1‑140 (MQ=255)
                                      cccaGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                                                                                           >  1:793695/1‑140 (MQ=255)
                                         aGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGa                                                                                        >  1:352565/1‑140 (MQ=255)
                                         aGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGa                                                                                        >  1:852746/1‑140 (MQ=255)
                                         aGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGa                                                                                        >  1:625120/1‑140 (MQ=255)
                                         aGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGa                                                                                        >  1:624037/1‑140 (MQ=255)
                                         aGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACAACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATACATGACGTTTTTGAGGACCTgt                                                                                        >  1:1020953/1‑139 (MQ=255)
                                           tCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGt                                                                                      <  2:537098/140‑1 (MQ=255)
                                           tCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGt                                                                                      >  1:106248/1‑140 (MQ=255)
                                           tCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGt                                                                                      <  2:536487/140‑1 (MQ=255)
                                           tCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGt                                                                                      <  2:1050422/140‑1 (MQ=255)
                                                cAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCa                                                                                 <  2:338706/140‑1 (MQ=255)
                                                     cGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCtt                                                                            >  2:1125916/1‑140 (MQ=255)
                                                        tAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTgcg                                                                         <  2:982556/140‑1 (MQ=255)
                                                            tttCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTcc                                                                     <  2:378549/140‑1 (MQ=255)
                                                                      tGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTggg                                                           <  1:1030354/140‑1 (MQ=255)
                                                                              gaCAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACg                                                   <  2:361275/140‑1 (MQ=255)
                                                                              gaCAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACg                                                   <  1:702771/140‑1 (MQ=255)
                                                                                  aaCGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTAGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGttct                                               >  2:79541/1‑140 (MQ=255)
                                                                                  aaCGCCATCACCACGTGTGGCAGCAGGGTGGGCGTGGCGATAGTAAAGCCACGGCGCGCGCCCTCGATAAACATATCCATGACGTTTTTGCGGACCTCAGTTGACATGCTTGCGCTCCTTGCGTTGGGTTGCGACGttct                                               >  2:352543/1‑140 (MQ=255)
                                                                                   aCGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGttctt                                              >  1:118921/1‑140 (MQ=255)
                                                                                   aCGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGttctt                                              >  1:198830/1‑140 (MQ=255)
                                                                                         tCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGt                                        >  1:1017254/1‑140 (MQ=255)
                                                                                                       agcagGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACgcgc                          >  1:476426/1‑140 (MQ=255)
                                                                                                                gTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTAGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTg                 >  2:1158157/1‑140 (MQ=255)
                                                                                                                       cGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGa          <  2:745317/140‑1 (MQ=255)
                                                                                                                           aGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACaaa      >  2:455517/1‑140 (MQ=255)
                                                                                                                               aaGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTa  <  1:222898/140‑1 (MQ=255)
                                                                                                                               aaGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTa  <  1:525717/140‑1 (MQ=255)
                                                                                                                               aaGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTa  <  1:720614/140‑1 (MQ=255)
                                                                                                                               aaGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTa  <  1:1018935/140‑1 (MQ=255)
                                                                                                                               aaGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTa  <  1:83488/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
TCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTA  >  NC_000913/4559812‑4560078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑