Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 513,920 C→A 5.6% intergenic (+52/‑73) ybaT → / → cueR putative amino acid transporter/copper‑responsive regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913513,9200CA 5.6% 254.5 / 2.6 71intergenic (+52/‑73)ybaT/cueRputative amino acid transporter/copper‑responsive regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (42/25);  new base A (2/2);  total (44/27)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

GGGTAGCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGA  >  NC_000913/513781‑514059
                                                                                                                                           |                                                                                                                                           
gggTAGCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTc                                                                                                                                             >  2:1188297/1‑140 (MQ=255)
     gCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACt                                                                                                                                        >  1:673913/1‑140 (MQ=255)
     gCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACt                                                                                                                                        >  1:673909/1‑140 (MQ=255)
     gCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGGCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACt                                                                                                                                        >  1:533203/1‑140 (MQ=255)
      ccGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTg                                                                                                                                       <  1:240818/140‑1 (MQ=255)
          gCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCaa                                                                                                                                   >  1:609722/1‑140 (MQ=255)
          gCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCaa                                                                                                                                   >  1:609716/1‑140 (MQ=255)
               gATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATTGTCAAGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCaaaaaaa                                                                                                                              <  2:1034093/140‑1 (MQ=255)
               gATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCaaaaaaa                                                                                                                              <  2:1034175/140‑1 (MQ=255)
                atatGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCaaaaaatc                                                                                                                             >  2:1072354/1‑138 (MQ=255)
                atatGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAAc                                                                                                                             >  2:448424/1‑140 (MQ=255)
                     gATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCt                                                                                                                        <  2:971039/140‑1 (MQ=255)
                     gATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCt                                                                                                                        <  2:601847/140‑1 (MQ=255)
                        tattGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGa                                                                                                                     >  1:93369/1‑140 (MQ=255)
                        tattGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGa                                                                                                                     >  1:322972/1‑140 (MQ=255)
                          ttGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAat                                                                                                                   >  1:266443/1‑140 (MQ=255)
                          ttGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAat                                                                                                                   >  2:385247/1‑140 (MQ=255)
                            tgCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTATTTTCTAAGTAATTCATTTATTATTTTGGTTGATTTCGTTATTCTAAATGTAAAAAAAACGTGCTTGTatat                                                                                                                 <  2:249287/139‑1 (MQ=255)
                             gCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATc                                                                                                                <  2:381906/140‑1 (MQ=255)
                             gCTCACTCTTACACAGCCTTATTGTTGCAATGGTCCTGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAATAAACGTGATTGAATATc                                                                                                                <  2:380008/140‑1 (MQ=255)
                               tCACTCTTACTCAGCCTTATTGTGGCTATGGTCATGTAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACGTCCTGTATTATTGTGGAGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTg                                                                                                              >  2:889966/1‑140 (MQ=255)
                               tCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAAAAAAACCGTATAACATCTCTCAGTGCGCAGTACTTCCTGTATTATTGAGGTGGCGGACGATATTCGCACTTGCAAAAAAACGTGCTTTAATATCTg                                                                                                              >  2:890257/1‑140 (MQ=255)
                                cACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGt                                                                                                             >  1:218400/1‑140 (MQ=255)
                                   tctTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGa                                                                                                          <  1:646116/140‑1 (MQ=255)
                                   tctTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGa                                                                                                          <  1:645822/140‑1 (MQ=255)
                                       aCTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAAcc                                                                                                      >  2:1198906/1‑140 (MQ=255)
                                       aCTCAGCCTTATTGTGGCAATGGTAATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTAGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAAcc                                                                                                      >  1:1117333/1‑140 (MQ=255)
                                          cAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCtt                                                                                                   <  1:479798/140‑1 (MQ=255)
                                          cAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCtt                                                                                                   <  1:92430/140‑1 (MQ=255)
                                          cAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCtt                                                                                                   <  1:92798/140‑1 (MQ=255)
                                           aGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCttt                                                                                                  >  2:697649/1‑140 (MQ=255)
                                           aGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGAGGCGCTCGATATTCGCACTGGCAAAAAAACGAGCTTGAATATCTGATGAAACCCttt                                                                                                  >  2:697645/1‑140 (MQ=255)
                                             ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGGTTGTATATCTGTTGTAACCCTTTaa                                                                                                >  1:387301/1‑140 (MQ=255)
                                             ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTaa                                                                                                >  2:174838/1‑140 (MQ=255)
                                             ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTaa                                                                                                >  2:639519/1‑140 (MQ=255)
                                             ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTaa                                                                                                >  1:1079619/1‑140 (MQ=255)
                                             ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTaa                                                                                                >  1:197588/1‑140 (MQ=255)
                                             ccTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTaa                                                                                                >  1:567917/1‑140 (MQ=255)
                                               ttATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACa                                                                                              <  1:230951/140‑1 (MQ=255)
                                                       gCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACag                                                                                      >  2:886086/1‑140 (MQ=255)
                                                       gCAATGGTAATGAATCGCAAAAAAACAGTATAACATCTCTCTGTGCGCAGTACTTCCTGTAATATAGTGGTGGCGGTCGATAATCGCACTGGCAAAAAAACCTGCTTGAATAACTGATGAAACCCTTGAACAAAGCACag                                                                                      >  2:1096799/1‑140 (MQ=255)
                                                              tCATGAAGCGCAATAAAACCGTATATCATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGt                                                                               <  2:609716/140‑1 (MQ=255)
                                                              tCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGt                                                                               <  2:609722/140‑1 (MQ=255)
                                                                    aGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGcgcg                                                                         <  1:138605/140‑1 (MQ=255)
                                                                    aGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGcgcg                                                                         <  1:138712/140‑1 (MQ=255)
                                                                    aGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGcgcg                                                                         <  2:342412/140‑1 (MQ=255)
                                                                        cAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACg                                                                     >  1:91735/1‑140 (MQ=255)
                                                                        cAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAAAGTGGTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACg                                                                     >  2:766593/1‑140 (MQ=255)
                                                                              aaCCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCTATATTCGCACTGGAAAAAAAACGTGATTTAATATCTGTTGAAACCATTTAAAAAAGCACAGGAGGCGTTGCGCGAACTATGAAc                                                               <  1:424080/140‑1 (MQ=255)
                                                                                   tataACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAg                                                          >  1:154941/1‑140 (MQ=255)
                                                                                          tctctcTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTa                                                   >  2:835279/1‑140 (MQ=255)
                                                                                          tctctcTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTa                                                   >  2:835325/1‑140 (MQ=255)
                                                                                          tctctcTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTa                                                   >  2:915693/1‑140 (MQ=255)
                                                                                          tctctcTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTa                                                   >  2:1029475/1‑140 (MQ=255)
                                                                                           ctctctGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGCTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAg                                                  >  1:326554/1‑140 (MQ=255)
                                                                                                  tgCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAAt                                           >  1:1062987/1‑140 (MQ=255)
                                                                                                        gTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACa                                                                                              <  1:34825/83‑1 (MQ=255)
                                                                                                        gTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACa                                                                                              >  2:34825/1‑83 (MQ=255)
                                                                                                         tACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTAGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGc                                    <  1:208144/140‑1 (MQ=255)
                                                                                                                   attattGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCa                          >  2:201200/1‑140 (MQ=255)
                                                                                                                     tattGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCaaa                        >  2:1154984/1‑140 (MQ=255)
                                                                                                                           ggtggCGGTCGATATTCGCTCTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCAAtt                  <  1:761141/140‑1 (MQ=255)
                                                                                                                           ggtggCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCAtt                  <  2:413095/140‑1 (MQ=255)
                                                                                                                            gtggCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTc                 >  1:977846/1‑140 (MQ=255)
                                                                                                                            gtggCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTc                 >  2:507443/1‑140 (MQ=255)
                                                                                                                            gtggCGGTCGATATTAGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGaa                                                                <  2:769531/93‑1 (MQ=255)
                                                                                                                            gtggCGGTCGATATTAGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGaa                                                                >  1:769607/1‑93 (MQ=255)
                                                                                                                            gtggCGGTCGATATTAGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGaa                                                                >  1:769531/1‑93 (MQ=255)
                                                                                                                            gtggCGGTCGATATTAGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGaa                                                                <  2:769607/93‑1 (MQ=255)
                                                                                                                             tggCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCg                <  1:135810/140‑1 (MQ=255)
                                                                                                                               gCGGTCGATATTCGCACTGGCAAAAAATCGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAATAATTACCGGCCTGACCAGCAAAGCCATTCGCt              >  2:424841/1‑140 (MQ=255)
                                                                                                                                 ggTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGTAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCTAAAATTACCGGCCTGACCAGCAAAGCCATTCGCttg            >  1:791081/1‑139 (MQ=255)
                                                                                                                                           cGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAgaga  <  1:201200/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GGGTAGCCGTGCGTTGATATGGATTATTGGCTCACTCTTACTCAGCCTTATTGTGGCAATGGTCATGAAGCGCAATAAAACCGTATAACATCTCTCTGTGCGCAGTACTTCCTGTATTATTGTGGTGGCGGTCGATATTCGCACTGGCAAAAAAACGTGCTTGAATATCTGTTGAAACCCTTTAACAAAGCACAGGAGGCGTTGCGCGAACGATGAACATCAGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGA  >  NC_000913/513781‑514059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: