Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	702,583	C→T	5.1%	D57N (GAT→AAT)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,583	0	C	T	5.1%	282.1 / 3.8	79	D57N (GAT→AAT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base C (31/44);  new base T (2/2);  total (33/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTA  >  NC_000913/702447‑702722                                                                                                                                         |                                                                                                                                            gtggtgATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACTCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGAt                                                                                                                                          >  2:250246/1‑140 (MQ=255) gtggtgATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGAt                                                                                                                                          <  1:593875/140‑1 (MQ=255) gtggtgATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGAt                                                                                                                                          <  1:594858/140‑1 (MQ=255) gtggtgATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGAt                                                                                                                                          >  1:60190/1‑140 (MQ=255) gtggtgATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGAt                                                                                                                                          >  2:250225/1‑140 (MQ=255) gtggtgATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGAt                                                                                                                                          >  1:18947/1‑140 (MQ=255)      gATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAAc                                                                                                                                     <  1:1108605/140‑1 (MQ=255)           gCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCgggg                                                                                                                                <  2:1138742/140‑1 (MQ=255)           gCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCGTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCgggg                                                                                                                                <  2:759425/140‑1 (MQ=255)            cGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCggggg                                                                                                                               <  1:1005965/140‑1 (MQ=255)            cGTCGGCAGATAGTTAGTACAGCCTGATGTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCggggg                                                                                                                               <  1:1005919/140‑1 (MQ=255)             gTCGGCAGATATTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAAACGGGGga                                                                                                                              <  2:153097/140‑1 (MQ=255)             gTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGga                                                                                                                              <  2:218484/140‑1 (MQ=255)             gTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGga                                                                                                                              <  2:152959/140‑1 (MQ=255)             gTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGga                                                                                                                              <  2:153248/140‑1 (MQ=255)             gTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGga                                                                                                                              <  2:938142/140‑1 (MQ=255)             gTCGGCAGATAGTGAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTCCGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGga                                                                                                                              <  2:458837/140‑1 (MQ=255)                  cAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGaa                                                                                                                         <  1:1038439/140‑1 (MQ=255)                               cAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAg                                                                                                            >  2:911270/1‑140 (MQ=255)                               cAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAg                                                                                                            >  1:59481/1‑140 (MQ=255)                                 gCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTg                                                                                                          >  1:1074012/1‑140 (MQ=255)                                  ccTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGa                                                                                                         >  2:1136176/1‑140 (MQ=255)                                  ccTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGa                                                                                                         >  1:468604/1‑140 (MQ=255)                                      aTTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACgt                                                                                                     >  2:415347/1‑140 (MQ=255)                                      aTTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACgt                                                                                                     >  2:415426/1‑140 (MQ=255)                                      aTTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACgt                                                                                                     >  2:1057226/1‑140 (MQ=255)                                       tttCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACgtt                                                                                                    >  2:1009804/1‑140 (MQ=255)                                         tctcATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACgttgt                                                                                                  <  1:89807/140‑1 (MQ=255)                                         tctcATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACgttgt                                                                                                  <  1:790048/140‑1 (MQ=255)                                           tcATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTc                                                                                                <  2:417113/140‑1 (MQ=255)                                            cATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCg                                                                                               >  1:711088/1‑140 (MQ=255)                                                  cTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATctct                                                                                         >  2:1213738/1‑140 (MQ=255)                                                        gCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTggcggc                                                                                   <  1:719991/140‑1 (MQ=255)                                                        gCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTggcggc                                                                                   <  1:720014/140‑1 (MQ=255)                                                        gCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTggcggc                                                                                   <  2:114871/140‑1 (MQ=255)                                                        gCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTggcggc                                                                                   <  2:113231/140‑1 (MQ=255)                                                        gCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTggcggc                                                                                   <  1:121901/140‑1 (MQ=255)                                                             aTTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTc                                                                              <  2:1021601/140‑1 (MQ=255)                                                             aTTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTc                                                                              <  2:461450/140‑1 (MQ=255)                                                                  cAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAACCCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTa                                                                         <  1:1200288/140‑1 (MQ=255)                                                                  cAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTa                                                                         <  1:1046110/140‑1 (MQ=255)                                                                   aGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTAc                                                                        >  2:851302/1‑140 (MQ=255)                                                                        ttCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGAc                                                                   >  2:751980/1‑140 (MQ=255)                                                                           cACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGa                                                                <  2:300420/140‑1 (MQ=255)                                                                           cACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGa                                                                <  1:588619/140‑1 (MQ=255)                                                                           cACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGa                                                                <  2:149309/140‑1 (MQ=255)                                                                           cACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGa                                                                <  1:224047/140‑1 (MQ=255)                                                                           cACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGa                                                                <  1:496123/140‑1 (MQ=255)                                                                           cACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGa                                                                <  1:945377/140‑1 (MQ=255)                                                                           cACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGa                                                                <  2:464829/140‑1 (MQ=255)                                                                           cACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGa                                                                <  1:628843/140‑1 (MQ=255)                                                                                    cGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTaa                                                       >  2:205898/1‑140 (MQ=255)                                                                                     gCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAAt                                                      <  1:1136176/140‑1 (MQ=255)                                                                                            cGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCa                                               >  1:755141/1‑140 (MQ=255)                                                                                               tgtCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCa                                            <  1:803551/140‑1 (MQ=255)                                                                                                 tCGTTAAACTGTACGCCGCCGCAGCCTTATAACTGCACATCGATAAAACCTGGGGAGAGAATGGCCCCGTTCAGTGAACGTTTTTAGATCTCTGGCGGCAGTTCCGCAACCGGACAGACGCTTTTAATCAGGCCATCAGc                                          >  2:1053551/1‑140 (MQ=255)                                                                                                 tCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGc                                          <  2:963263/140‑1 (MQ=255)                                                                                                    ttAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGAt                                       <  1:184347/140‑1 (MQ=255)                                                                                                    ttAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGAt                                       >  2:768201/1‑140 (MQ=255)                                                                                                            tACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACcg                               >  1:392595/1‑140 (MQ=255)                                                                                                            tACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACcg                               >  2:949101/1‑140 (MQ=255)                                                                                                            tACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAAAcg                               >  1:930550/1‑140 (MQ=255)                                                                                                              cgccgccgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACcgcg                             >  1:623166/1‑140 (MQ=255)                                                                                                                   ccgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGtc                        >  1:462891/1‑140 (MQ=255)                                                                                                                   ccgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGtc                        >  1:462950/1‑140 (MQ=255)                                                                                                                   ccgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGtc                        >  1:463199/1‑140 (MQ=255)                                                                                                                           gTTTAACTGCACATTGATAAAACCGGGGGAGAGAGTGGCCCCg                                                                                                                 <  1:563644/43‑1 (MQ=255)                                                                                                                           gTTTAACTGCACATTGATAAAACCGGGGGAGAGAGTGGCCCCg                                                                                                                 >  2:563644/1‑43 (MQ=255)                                                                                                                           gTTTAACTGCACATTGATAAAACCGGGGGAGAGAATGGCCCCg                                                                                                                 <  1:563598/43‑1 (MQ=255)                                                                                                                           gTTTAACTGCACATTGATAAAACCGGGGGAGAGAATGGCCCCg                                                                                                                 >  2:563598/1‑43 (MQ=255)                                                                                                                                   gCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTgg        >  1:255031/1‑140 (MQ=255)                                                                                                                                   gCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTgg        >  1:567798/1‑140 (MQ=255)                                                                                                                                    cacaTCGATCAACCCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCTTCAAGAAATTCGTGGc       <  2:1104542/140‑1 (MQ=255)                                                                                                                                    cacaTCGATAACACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGc       <  1:1103120/140‑1 (MQ=255)                                                                                                                                    cacaTCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACCACCGCGTGGTCATCAAGAAATTCGTGGc       <  1:1103163/140‑1 (MQ=255)                                                                                                                                    cacaTCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGc       <  2:60190/140‑1 (MQ=255)                                                                                                                                    cacaTCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGc       >  2:101285/1‑140 (MQ=255)                                                                                                                                    cacaTCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGc       <  1:139769/140‑1 (MQ=255)                                                                                                                                    cacaTCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGc       <  1:454608/140‑1 (MQ=255)                                                                                                                                         cGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTa  <  1:795233/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                            GTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTA  >  NC_000913/702447‑702722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑