Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 845,660 C→A 5.6% intergenic (‑180/+81) ybiO ← / ← glnQ mechanosensitive channel protein, intermediate conductance/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913845,6600CA 5.6% 255.3 / 2.5 72intergenic (‑180/+81)ybiO/glnQmechanosensitive channel protein, intermediate conductance/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (43/25);  new base A (2/2);  total (45/27)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

ATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAACA  >  NC_000913/845522‑845799
                                                                                                                                          |                                                                                                                                           
aTGCGGGAGGTGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGGGAAAGGGAGGAAGGGTTATTAAGCAGGTTGCTGACAACGTGCGGGCGTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                                                                                            >  2:454546/1‑140 (MQ=39)
aTGCGGGAGGAGGGGCGATTTTAAATGAGAGGATTCTGGTGTGCCTCCCTTTCGGGTGATAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACCTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                            >  2:454537/1‑140 (MQ=39)
 tGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCt                                                                                                                                           >  1:467641/1‑140 (MQ=255)
     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCTTCCTACaa                                                                                                                                       >  1:58173/1‑140 (MQ=255)
     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                                                                                                       >  2:922999/1‑140 (MQ=255)
     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                                                                                                       >  1:646218/1‑140 (MQ=255)
     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                                                                                                       >  2:556745/1‑140 (MQ=255)
     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                                                                                                       >  1:204142/1‑140 (MQ=255)
     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                                                                                                       >  1:204245/1‑140 (MQ=255)
     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                                                                                                       >  2:556758/1‑140 (MQ=255)
     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTATTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                                                                                                       >  1:646379/1‑140 (MQ=255)
            gggCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTACTACAAGTTCATg                                                                                                                                >  1:1140556/1‑140 (MQ=255)
            gggCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTACTACAAGTTCATg                                                                                                                                >  1:1141361/1‑140 (MQ=255)
                           agagGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGtt                                                                                                                 <  2:277386/140‑1 (MQ=255)
                           agagGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGtt                                                                                                                 <  2:943987/140‑1 (MQ=255)
                             agGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGc                                                                                                               >  1:1034440/1‑140 (MQ=255)
                              ggAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGcc                                                                                                              <  1:186772/140‑1 (MQ=255)
                                 aTCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGa                                                                                                           <  2:1018402/140‑1 (MQ=255)
                                  tCTGGTGTGCCTCCCTTTCGGGTGTAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGACTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGcctgtt                                                                                                          >  2:851132/1‑136 (MQ=255)
                                  tCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGat                                                                                                          <  1:171478/140‑1 (MQ=255)
                                   cTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGata                                                                                                         <  2:200054/140‑1 (MQ=255)
                                        cttccTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTg                                                                                                    >  2:394917/4‑140 (MQ=255)
                                             tCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGc                                                                                               <  1:582774/140‑1 (MQ=255)
                                                  ttCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGAt                                                                                          <  1:931752/140‑1 (MQ=255)
                                                  ttCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGAt                                                                                          <  2:60341/140‑1 (MQ=255)
                                                        tGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGt                                                                                    >  2:448687/1‑140 (MQ=255)
                                                        tGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGt                                                                                    >  2:819104/1‑140 (MQ=255)
                                                        tGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGt                                                                                    >  1:891940/1‑140 (MQ=255)
                                                               gaggaAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCAt                                                                             <  2:204245/140‑1 (MQ=255)
                                                               gaggaAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCAt                                                                             <  2:204142/140‑1 (MQ=255)
                                                                       gATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                                                                     <  2:116411/140‑1 (MQ=255)
                                                                       gATATAAAGCTGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATAACCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                                                                     <  2:116735/140‑1 (MQ=255)
                                                                        atatTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAtg                                                                    >  2:933154/1‑140 (MQ=255)
                                                                          atTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAtgtg                                                                  >  1:1057271/1‑140 (MQ=255)
                                                                          atTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAtgtg                                                                  >  1:532539/1‑140 (MQ=255)
                                                                            tAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTc                                                                >  1:142085/1‑140 (MQ=255)
                                                                            tAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTc                                                                >  2:639133/1‑140 (MQ=255)
                                                                              atcAGATTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCtt                                                              <  2:1025433/138‑1 (MQ=255)
                                                                              aGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCtt                                                              <  2:467641/140‑1 (MQ=255)
                                                                                 aGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCttatt                                                           >  2:935177/1‑140 (MQ=255)
                                                                                   gTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTaa                                                         >  1:732704/1‑140 (MQ=255)
                                                                                     tGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAaga                                                       >  2:682633/1‑140 (MQ=255)
                                                                                        tGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGACCGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGAc                                                    >  1:738696/1‑140 (MQ=255)
                                                                                             aCGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCTGGCAATGTGTCTTATTAAGAGACGtgct                                               >  2:348062/1‑140 (MQ=255)
                                                                                             aCGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGtgct                                               >  1:80440/1‑140 (MQ=255)
                                                                                             aCGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGtgct                                               >  1:78144/1‑140 (MQ=255)
                                                                                             aCGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGtgct                                               <  2:1057271/140‑1 (MQ=255)
                                                                                              cGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGtgctg                                              >  2:663172/1‑140 (MQ=255)
                                                                                                         ttttGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCt                                   >  1:1115392/1‑140 (MQ=255)
                                                                                                          tttGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTg                                  >  2:915996/1‑140 (MQ=255)
                                                                                                          tttGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTg                                  >  2:915924/1‑140 (MQ=255)
                                                                                                          tttGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTg                                  >  2:915766/1‑140 (MQ=255)
                                                                                                          tttGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTg                                  >  2:915368/1‑140 (MQ=255)
                                                                                                                ggATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAgcg                            <  1:501264/140‑1 (MQ=255)
                                                                                                                 gATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAgcgc                           >  1:604213/1‑140 (MQ=255)
                                                                                                                     ctgcgTGTACGCCTTTTCCTTCCTACAAGTTCATGCTATTTTAATATGTTGCCTGATATCCTGTAGGCCTGATAAGCGTAGCGCTTCTGGCTTTGTGTATTATTAAGAGACTTGCTGCATAAATTCCTGCAAGCGCTGGc                       <  2:1104973/138‑1 (MQ=255)
                                                                                                                     cggcgTGCACGCCTTATCCGTCCTACTAGTTCATGCAATTTTTATAAGTTGCCAGATATCCTGTAGGCCTGATTAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGGAAAAATTCCTGCAAGCGCTGGc                       <  2:1104942/140‑1 (MQ=38)
                                                                                                                     cggcgTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGc                       <  1:639133/140‑1 (MQ=255)
                                                                                                                      ggcgTGAACGCCTTATCCGTACTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCt                      <  2:1141361/140‑1 (MQ=255)
                                                                                                                      ggcgTGAACGCCTTATCCGTACTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCt                      <  2:1140556/140‑1 (MQ=255)
                                                                                                                           gAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTcggcg                 <  1:1149876/140‑1 (MQ=255)
                                                                                                                                ccTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTc            <  1:912570/140‑1 (MQ=255)
                                                                                                                                 cTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCt           <  1:663172/140‑1 (MQ=255)
                                                                                                                                 cTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCt           <  2:732704/140‑1 (MQ=255)
                                                                                                                                   tATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTg         <  1:1148196/140‑1 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATGTCCTGTAGGCCTGAGAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAATTTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  1:716414/1‑140 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  1:109190/1‑140 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  1:123056/1‑140 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  1:550624/1‑140 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  1:628959/1‑140 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  2:370363/1‑140 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  2:309090/1‑140 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  1:716639/1‑140 (MQ=255)
                                                                                                                                         tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAac   >  2:1152848/1‑140 (MQ=255)
                                                                                                                                          ccTACATGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAaca  >  2:197580/1‑140 (MQ=255)
                                                                                                                                          ccTACAAGTTCATGCAATTTTAAAATGTTGCCCGATATCCTGTTGGCCTGATAAGCGTCGCGCAGCCGGGATTGTGTCTTATTATGTGTCGTGCTGCAGTAATTCCTGCTAGAGCTGGCTCGGCGGGTTCTAGAtgatca  >  2:1210570/1‑135 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAACA  >  NC_000913/845522‑845799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: