Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,173,753 G→A 6.5% A71V (GCG→GTG)  mfd ← transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,173,7530GA 6.5% 208.2 / 3.6 61A71V (GCG→GTG) mfdtranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (38/19);  new base A (2/2);  total (40/21)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTACCAGCGTTGCACAGGC  >  NC_000913/1173616‑1173889
                                                                                                                                         |                                                                                                                                        
cgcgCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGcc                                                                                                                                        <  2:486682/140‑1 (MQ=255)
cgcgCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGcc                                                                                                                                        <  2:486644/140‑1 (MQ=255)
     tGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGAtt                                                                                                                                   >  1:1135935/1‑140 (MQ=255)
             cGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCa                                                                                                                           >  2:176430/1‑140 (MQ=255)
               tATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCAtt                                                                                                                         >  2:367103/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCagatg                                                                                                                       >  2:837823/1‑136 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                                                                                       >  1:967846/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                                                                                       >  2:603620/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                                                                                       >  2:581088/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                                                                                       >  2:354109/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                                                                                       >  2:307308/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                                                                                       >  1:943316/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                                                                                       >  1:602423/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTg                                                                                                                       >  1:288887/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCACCAGATTCATCACCATTTg                                                                                                                       >  1:926212/1‑140 (MQ=255)
                 ttCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCACCAGATTCATCACCATTTg                                                                                                                       >  1:926174/1‑140 (MQ=255)
                      cGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCgg                                                                                                                  >  2:26623/1‑140 (MQ=255)
                                          gCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTtcatca                                                                                              >  2:220976/1‑140 (MQ=255)
                                            tGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGATAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATg                                                                                            >  2:788394/1‑140 (MQ=255)
                                               ttggtcgGTAGCTGGTAATGGGTGGAATGGCGCGAGGAGATACTGTCCTGATGAGGCGAAATTCTGTCGTAGGGATGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAATCTGGCTGATTTCATCATGCaa                                                                                         <  1:837823/137‑1 (MQ=255)
                                                tcgtcgGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCaaa                                                                                        >  2:676450/1‑140 (MQ=255)
                                                   tcgGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGc                                                                                     >  1:869210/1‑140 (MQ=255)
                                                    cgGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCa                                                                                    <  2:386759/140‑1 (MQ=255)
                                                    cgGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCa                                                                                    <  2:1176686/140‑1 (MQ=255)
                                                     ggTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCag                                                                                   >  1:579638/1‑140 (MQ=255)
                                                        aGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGc                                                                                >  1:125897/1‑140 (MQ=255)
                                                        aGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGc                                                                                >  1:604663/1‑140 (MQ=255)
                                                        aGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGc                                                                                >  2:1092689/1‑140 (MQ=255)
                                                        aGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGc                                                                                >  2:137770/1‑140 (MQ=255)
                                                        aGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGc                                                                                >  2:137834/1‑140 (MQ=255)
                                                                   ggTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCatat                                                                     <  2:962828/140‑1 (MQ=255)
                                                                   ggTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCatat                                                                     <  2:962873/140‑1 (MQ=255)
                                                                    gTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCaa                                                                                         <  2:637685/119‑1 (MQ=255)
                                                                    gTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCaa                                                                                         >  1:637685/1‑119 (MQ=255)
                                                                       gAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTgg                                                                 >  1:121438/1‑140 (MQ=255)
                                                                       gAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTgg                                                                 >  1:120966/1‑140 (MQ=255)
                                                                       gAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTgg                                                                 >  1:719976/1‑140 (MQ=255)
                                                                           ggCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCa                                                             <  1:581088/140‑1 (MQ=255)
                                                                             cgcgAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAAt                                                           >  2:330179/1‑140 (MQ=255)
                                                                             cgcgAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAAt                                                           <  1:1151106/140‑1 (MQ=255)
                                                                             cgagaggagATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGAATTTTGCATATCTGGTGCAAt                                                           <  1:1150496/137‑1 (MQ=255)
                                                                               cgAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGa                                                         >  1:27564/1‑140 (MQ=255)
                                                                                 aggagATACTGTCCGGATGAGGCGAAAAACTGTCGTAGGGACGAGTGTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGt                                                       <  1:1168358/140‑1 (MQ=255)
                                                                                 aggagATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATATGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGt                                                       >  1:815063/1‑140 (MQ=255)
                                                                                      aTAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTaccac                                                  >  1:307813/1‑140 (MQ=255)
                                                                                                gatgaGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCg                                        >  2:877397/1‑140 (MQ=255)
                                                                                                             aCTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCaa                           <  1:543646/140‑1 (MQ=255)
                                                                                                                gTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAAttt                        >  2:965362/1‑140 (MQ=255)
                                                                                                                  cGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTcc                      <  2:565140/140‑1 (MQ=255)
                                                                                                                       ggAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTAc                 <  2:602423/140‑1 (MQ=255)
                                                                                                                       ggAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTAc                 <  2:108018/140‑1 (MQ=255)
                                                                                                                       ggAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTAc                 <  1:1009185/140‑1 (MQ=255)
                                                                                                                       ggAAGAGTTTCCCAGTCCACCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTAc                 <  2:926174/140‑1 (MQ=255)
                                                                                                                       ggAAGAGTTTCCCAGTCCACCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTAc                 <  2:926212/140‑1 (MQ=255)
                                                                                                                                 cccAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCACTGAGTACCACCGGACCGGCGTGACGTTCGGCCATGTCCGCTACCAGCGTTGca       >  2:445326/1‑140 (MQ=255)
                                                                                                                                 cccAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTACCAGCGTTGca       >  2:699784/1‑140 (MQ=255)
                                                                                                                                 cccAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTACCAGCGTTGca       <  2:951541/140‑1 (MQ=255)
                                                                                                                                 cccAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTACCAGCGTTGca       <  2:168626/140‑1 (MQ=255)
                                                                                                                                 cccAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCAGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTACCAGCGTAGca       >  2:699812/1‑140 (MQ=255)
                                                                                                                                      tCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTACCAGCGTTGCACAGGc  >  2:867069/1‑140 (MQ=255)
                                                                                                                                      tCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTACCAGCGTTGCACAGGc  >  2:189960/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CGCGCTGCATAAGCGTATTCACCGGAACAATCAGTACGCCACGCTGCATCGTCGGTAGCTGGTAAAGGGTGGAAAGGCGCGAGGAGATAATGTCCTGATGAGGCGAAAAACTGTCGTAGGGAAGAGTTTCCCAGTCCGCCAGATTCATCACCATTTGATCGGTAAACTGGCTGATTTCATCATGCAAACGCAGAGCATTTTGCATATCTGGTGCAATGAGTACCACCGGACCGGCGTGACGTTCGGCAATTTCCGCTACCAGCGTTGCACAGGC  >  NC_000913/1173616‑1173889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑