| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 211,354 | C→A | 6.1% | S559Y (TCT→TAT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 211,354 | 0 | C | A | 6.1% | 228.0 / 2.8 | 65 | S559Y (TCT→TAT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (37/24); new base A (2/2); total (39/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
GGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTT > NC_000913/211217‑211491 | ggTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCtt < 2:1013300/140‑1 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTa < 2:140705/140‑1 (MQ=255) aGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACg < 2:754970/140‑1 (MQ=255) tGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCaaaa < 2:516620/140‑1 (MQ=255) tatagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAATACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTAccc < 2:1187444/137‑1 (MQ=255) tagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTAccc < 1:912986/140‑1 (MQ=255) gagaAAACCGGCCCTTATTACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAATCGCTCTTACGATCTCAACCTGCGGATCAAAAGTATGCTTCCCGa > 1:768363/1‑140 (MQ=255) agaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAt < 2:192905/140‑1 (MQ=255) agaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTATTACGATCTCAACCTGCGGATCAAAAATATGc < 1:1201957/132‑1 (MQ=255) agaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTATTACGATCTCAACCTGCGGATCAAAAATATGc > 2:1201957/1‑132 (MQ=255) agaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTATTACGATCTCAACCTGCGGATCAAAAATATGc > 2:1201752/1‑132 (MQ=255) agaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTATTACGATCTCAACCTGCGGATCAAAAATATGc < 1:1201752/132‑1 (MQ=255) gaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAtc > 1:451434/1‑140 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAtct > 1:904829/1‑140 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAtct > 2:849006/1‑140 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAtct < 2:683014/140‑1 (MQ=255) aaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGctctct > 2:1216700/1‑140 (MQ=255) aaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATctct > 1:218937/1‑140 (MQ=255) aCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTa > 2:602015/1‑140 (MQ=255) aCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTa > 2:600462/1‑140 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATc < 1:330211/140‑1 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAATCCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 1:374165/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 2:24424/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 1:332760/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 1:355934/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 2:941794/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 1:355985/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 2:744761/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 1:912000/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 1:898468/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 1:537724/1‑140 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGAttt > 1:562610/1‑140 (MQ=255) ttAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAt > 2:136890/1‑107 (MQ=255) ttAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAt < 1:136890/107‑1 (MQ=255) tGGCATCGATAAAACCAAAGCAATGGGATTATTCCGTGGGTGGACGGAATTCAAACGCACTTACGCTCTCAACCAGCGGATCAAAAATATGCTACCCGATCTCTCTGCAGAAGATCCCGATTTCTACCGCACTATGCGTa > 2:411374/1‑140 (MQ=255) gCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTAtt > 2:1158029/1‑140 (MQ=255) gCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTAtt > 2:131875/1‑140 (MQ=255) gCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTAtt > 1:687860/1‑140 (MQ=255) gCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGAGTAtt > 2:1117902/1‑140 (MQ=255) gATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCa < 2:226878/102‑1 (MQ=255) gATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCa > 1:226878/1‑102 (MQ=255) gATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGa > 2:1161584/1‑140 (MQ=255) gATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGa > 2:1161587/1‑140 (MQ=255) gATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGa > 2:408508/1‑140 (MQ=255) cAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGcac < 2:807790/140‑1 (MQ=255) aaGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACaa > 2:992628/1‑140 (MQ=255) gCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAgg < 1:1224474/140‑1 (MQ=255) gCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAgg < 1:144538/140‑1 (MQ=255) gCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAgg < 1:744761/140‑1 (MQ=255) gCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAgg < 1:758487/140‑1 (MQ=255) tGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATc > 1:376640/1‑140 (MQ=255) tGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAtct < 1:975049/66‑1 (MQ=255) tGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGAtct > 2:975049/1‑66 (MQ=255) tGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTc > 1:1204112/1‑140 (MQ=255) tGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTc > 1:29254/1‑140 (MQ=255) tGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTc > 1:968108/1‑140 (MQ=255) tGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTc > 1:969454/1‑140 (MQ=255) tGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCAAAAGCTGATTc > 2:462702/1‑140 (MQ=255) cGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAacac < 1:973434/140‑1 (MQ=255) aaTTCAAACGCTCTAACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGAt < 1:996855/140‑1 (MQ=255) ttCAAACGCTCTTACTATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTATACACGATCt < 2:687852/140‑1 (MQ=255) ttCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCt < 2:687860/140‑1 (MQ=255) aaaCGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTGAACACGATCTTcc < 2:543083/140‑1 (MQ=255) aCGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCg < 2:854440/140‑1 (MQ=255) gCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGt < 1:433300/140‑1 (MQ=255) ctctTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGtt < 1:849006/140‑1 (MQ=255) ctctTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGtt > 1:373413/1‑140 (MQ=255) | GGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCTTACGATCTCAACCTGCGGATCAAAAATATGCTACCCGATCTCTATGCAGAAGATCCCGATTTCTACCGCAATATGCGTATTCAGGATCTGGCACAAGGGATCCATAAGCTGATTCGTAAACACGATCTTCCCGGTT > NC_000913/211217‑211491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |