Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,202 A→G 9.8% noncoding (35/171 nt) rttR ← rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,2020AG 9.8% 265.3 / 20.4 106noncoding (35/171 nt)rttRrtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base A (53/39);  new base G (8/2);  total (63/43)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 109 total aligned reads displayed.

GATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTC  >  NC_000913/1287064‑1287339
                                                                                                                                          |                                                                                                                                         
gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTaa                                                                                                                                          >  2:326/1‑140 (MQ=255)
gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTCGACTCGAACCTTTGTCCAAGCTTCACATCCATCCCCGCATGGGCAGAAAATTTaa                                                                                                                                          >  1:1115032/1‑140 (MQ=11)
gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACACTCGCAGGCGCACGAGTAGAACCTTTGTCGAAGCATCTCATCCATCCCCGCATTGGCAGAATATTTaa                                                                                                                                          >  1:1114651/1‑140 (MQ=11)
     tggtggGGGAAGGATTACTCAGCGCTGCGCACTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                                                                     <  1:493623/140‑1 (MQ=37)
                ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                                                                          <  1:767032/140‑1 (MQ=40)
                     aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGtt                                                                                                                     >  2:341178/1‑140 (MQ=255)
                     aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGtt                                                                                                                     >  2:341168/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGGAACGCACTCTCGCTGGCGCTCGAGTCGTACCTTGGTGGAAGCTTCTGATCCTTCCCCGCATGGGCAGTATTTTTTATTGCGGTTTGGGTGGGAAGTTGAgggt                                                                                                               >  1:365862/1‑139 (MQ=14)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTAATTGCGGGTTCGGTGGGAAGTTCAGGGa                                                                                                               >  2:332152/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                                               >  2:1079489/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                                               >  1:1231915/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                                               >  2:428086/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGGTCAGGGa                                                                                                               >  2:173032/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                                               >  2:1011045/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                                               >  2:71391/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTCAATGCGGATTCGTTGGGAAGTTCAGGGa                                                                                                               >  2:773414/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAAACATCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGTGAAGTTAAGGGa                                                                                                               >  1:1152433/1‑140 (MQ=14)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCGTACCCGCCTGGGCAGCAGATTTAATTGCGGATTCGGTGGGAAGTTCAGGGa                                                                                                               >  1:173721/1‑140 (MQ=14)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATACTTCCCCGCAGTGGCCGAAAATTTAATTGCGGATTCGTGGGGAAGTACAGCGa                                                                                                               >  2:552308/1‑140 (MQ=14)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCACGAGTCGAACCGTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTGCGGTTTCGTTGGGTAGTTCAGGGa                                                                                                               >  1:1231882/1‑140 (MQ=14)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAAGAGTTAATAGCGGATTCGGTGGGAAGGTCAGGGa                                                                                                               >  2:508950/1‑140 (MQ=14)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCAACCTTCCCCGCATGGGCCGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                                               >  2:552265/1‑140 (MQ=255)
                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAAGGGCCGAATATTTAATGGCGGGTTCGTTGGGTAGTTCAGGGa                                                                                                               >  2:589834/1‑140 (MQ=14)
                            gcTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCACTCGCAGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATCCTTACCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTAAGGGaa                                                                                                              >  2:882519/1‑139 (MQ=14)
                            gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTGGTTGGGAAGTTAAGGGAc                                                                                                              >  2:852627/1‑140 (MQ=255)
                            gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTAAGGGAc                                                                                                              >  2:777178/1‑140 (MQ=255)
                            gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGAc                                                                                                              >  2:901697/1‑140 (MQ=255)
                            gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCAACCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTGAGGGAc                                                                                                              >  2:882518/1‑140 (MQ=255)
                            gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGAc                                                                                                              >  2:852631/1‑140 (MQ=255)
                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCTTTGGGAAAAAATTTAATTTCGGATAGGTTGGGAAGTTAATGGACgttt                                                                                                          >  2:886531/1‑140 (MQ=14)
                                   gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                                                                       <  2:1139943/140‑1 (MQ=255)
                                      tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGt                                                                                                    <  2:524100/140‑1 (MQ=255)
                                       cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTg                                                                                                   <  1:731810/140‑1 (MQ=255)
                                       cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCATAATATTTAATTGCGGATTCGTTGGGAAGTTCAGTGACTTTTGAAAGTg                                                                                                   <  1:155924/140‑1 (MQ=255)
                                        gCCCTACGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAGCCGTCCCCGCATGGGCAGAATCTTTAATTGCGGATTCGAGGGGCAGTTCCCGGCCTTTTGAAAGTGa                                                                                                  <  2:714818/140‑1 (MQ=14)
                                          ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                                                                <  2:526049/140‑1 (MQ=255)
                                          ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAATTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                                                                <  2:526017/140‑1 (MQ=255)
                                                      tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                                                    <  1:418538/140‑1 (MQ=255)
                                                      tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                                                    <  1:945327/140‑1 (MQ=255)
                                                      tGCCTGCGGCAACGCTCTCTCGCTGGCGCACAAGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCGCTTGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                                                    <  1:1046385/140‑1 (MQ=14)
                                                      tGCCTGCGGCAACGCTCTCTCGCTGGCCCTCGAGGCGAACCGTGGTCGAAGCTTCACATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTAAGGGCCTTGTGAAAGTGATGGTGGTGGGGGaa                                                                                    >  2:748539/1‑140 (MQ=14)
                                                      tGCCAGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                                                    <  1:796644/140‑1 (MQ=255)
                                                           tcGGCAACGCTCTCTCGCTTGCGCTCGAGTCGAACCTTGGTCGAAGCTTCACAACCATCCCCGCAAGGGCAGAAAATTTAATTTCGGAATCGTTGGGAAAATCAGGGACTTTTTAAATTGATGGTGGTGGGGGAACGAtt                                                                               >  2:470937/2‑140 (MQ=14)
                                                                    ctctctCGCTGGCGCTCTTTTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                                                                      <  1:82585/140‑1 (MQ=255)
                                                                               gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                                                           >  1:249451/1‑140 (MQ=255)
                                                                                cgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGAc                                                          <  2:97956/140‑1 (MQ=255)
                                                                                  cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCTGACTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGAGTTTTGAAAGTGCTGGTGGTGGGGGAAGGATTCGCACCTTCGAAGTCGATGACgg                                                        <  2:402289/140‑1 (MQ=18)
                                                                                          gAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTa                                                <  1:1227199/140‑1 (MQ=255)
                                                                                            aCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa                                              <  1:203156/140‑1 (MQ=255)
                                                                                            aCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa                                              <  1:1035689/140‑1 (MQ=255)
                                                                                            aCCTTGGTCGAAGCTTCTCATCCTTCACCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGTACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa                                              <  1:247028/140‑1 (MQ=255)
                                                                                            aCCTTGGTCGAAGCTTCTCATCCTACCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa                                              <  2:142592/140‑1 (MQ=255)
                                                                                             ccTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg                                             <  2:1042304/140‑1 (MQ=255)
                                                                                                 ggTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                         >  2:973807/1‑140 (MQ=255)
                                                                                                 ggTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                         <  1:426800/140‑1 (MQ=255)
                                                                                                      aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTccc                                    >  1:1229387/1‑140 (MQ=255)
                                                                                                            ctcATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGc                              <  1:312097/140‑1 (MQ=255)
                                                                                                            ctcATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGc                              <  1:312217/140‑1 (MQ=255)
                                                                                                              taTCCTTCCCCGCATTTGCTTTATATTTTATAGAGTTTTAGTTGTGTAGTTCAGGGACTTTTGATAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCg                            <  1:748539/139‑1 (MQ=17)
                                                                                                              cATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCg                            <  1:547752/140‑1 (MQ=255)
                                                                                                              cATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCg                            <  1:548041/140‑1 (MQ=255)
                                                                                                              cATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCg                            <  1:1094376/140‑1 (MQ=255)
                                                                                                                 ccTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTc                         >  1:1198599/1‑140 (MQ=17)
                                                                                                                   ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACTGTCTTCTCGCTTTGGCCGCTCgg                       >  2:316027/1‑140 (MQ=17)
                                                                                                                   ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCgg                       >  2:926805/1‑140 (MQ=18)
                                                                                                                   ttCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTg                                                                                                   <  1:799803/64‑1 (MQ=255)
                                                                                                                   ttCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGTTGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCgg                       >  2:757602/1‑140 (MQ=255)
                                                                                                                   ttCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGTCCGCTCgg                       >  2:475314/1‑140 (MQ=255)
                                                                                                                   ttCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCgg                       >  2:701304/1‑140 (MQ=255)
                                                                                                                   ttCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCgg                       >  2:432471/1‑140 (MQ=255)
                                                                                                                   ttCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTGTGGCCGCTCgg                       >  2:71143/1‑140 (MQ=255)
                                                                                                                    tCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  1:582941/1‑140 (MQ=18)
                                                                                                                    tCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGCAGTTCCGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCTGAGTTCCAGTCGGCTCCCGGTGGCCGCTCggg                      >  2:323888/1‑140 (MQ=18)
                                                                                                                    tCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      <  2:476274/140‑1 (MQ=255)
                                                                                                                    tCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  1:167659/1‑140 (MQ=255)
                                                                                                                    tCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  1:167752/1‑140 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  2:887796/1‑140 (MQ=18)
                                                                                                                    tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  2:887898/1‑140 (MQ=18)
                                                                                                                    tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  2:401034/1‑140 (MQ=18)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCGCTTTGGCCGCTCtgg                      >  2:451365/1‑140 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  1:815175/1‑140 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  2:1009085/1‑140 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  2:152837/1‑140 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  2:507627/1‑140 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      <  2:476236/140‑1 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  2:451353/1‑140 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  2:395063/1‑140 (MQ=255)
                                                                                                                    tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                      >  1:737629/1‑140 (MQ=255)
                                                                                                                     ccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGa                     <  1:323888/140‑1 (MQ=255)
                                                                                                                     ccccGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGa                     <  1:324030/140‑1 (MQ=255)
                                                                                                                       ccGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc                   <  2:1102030/140‑1 (MQ=255)
                                                                                                                       ccGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc                   <  1:449615/140‑1 (MQ=255)
                                                                                                                       ccGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc                   <  1:45453/140‑1 (MQ=255)
                                                                                                                       ccGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc                   <  1:1079489/140‑1 (MQ=255)
                                                                                                                        cGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAcc                  <  2:1229387/140‑1 (MQ=255)
                                                                                                                          cATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAcccc                >  2:1076947/1‑140 (MQ=255)
                                                                                                                          cATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAcccc                <  1:213049/140‑1 (MQ=255)
                                                                                                                           aTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCcca               >  1:628797/1‑140 (MQ=255)
                                                                                                                           aTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCcca               >  1:628793/1‑140 (MQ=255)
                                                                                                                                        ttAATTGCGGATTCGTTGGGAAGTTCAGGGACATTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACATACCAATACGATGACGGCAGATATACAGACTGCTCCCATTGGCCCCTCGGGAACCCCACCCGGGGTAATTc  >  1:280367/1‑140 (MQ=21)
                                                                                                                                          |                                                                                                                                         
GATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTC  >  NC_000913/1287064‑1287339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: