Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,328,032 C→A 5.8% W109L (TGG→TTG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,328,0320CA 5.8% 285.0 / 2.7 77W109L (TGG→TTG) btuRcob(I)yrinic acid a,c‑diamide adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (47/25);  new base A (2/3);  total (49/28)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGCCTTTAATAAA  >  NC_000913/1327893‑1328168
                                                                                                                                           |                                                                                                                                        
cGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCAAATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGcc                                                                                                                                          >  1:383444/1‑140 (MQ=255)
    tGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGAc                                                                                                                                      >  1:629941/1‑140 (MQ=255)
            tgGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGc                                                                                                                              >  1:540481/1‑140 (MQ=255)
            tgGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGc                                                                                                                              >  1:540587/1‑140 (MQ=255)
              gACGTTCATTTAACGCCTGCACCACTTCTTCCAGGGGAAAATAGTCATTCGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCCTCCGCTTTGCATGTTGCCAGACTTCGCGGCAg                                                                                                                            <  2:567436/140‑1 (MQ=255)
               aCGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATCCGTCAGTTCATCAAGCAACCCCATCTCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAgg                                                                                                                           <  2:1005567/140‑1 (MQ=255)
                  ttCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAggcgg                                                                                                                        <  2:613207/140‑1 (MQ=255)
                   tCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAggcggc                                                                                                                       >  1:419754/1‑140 (MQ=255)
                   tCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAggcggc                                                                                                                       >  1:880593/1‑140 (MQ=255)
                                tcaccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTcg                                                                                                          <  2:638972/139‑1 (MQ=255)
                                gCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTcg                                                                                                          <  1:831771/140‑1 (MQ=255)
                                gCACCACTTCTTCCAGTGGCAAATAGTCAGACGCCACCATATACGTCAGTTCATCAAGCTAAACCATATCCAGTGAGCAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCTCGGCAGGCGGCGGTATCAGACTcg                                                                                                          <  2:1011546/140‑1 (MQ=255)
                                 caccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTcgc                                                                                                         >  2:563895/1‑140 (MQ=255)
                                 caccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTcgc                                                                                                         >  2:563292/1‑140 (MQ=255)
                                 caccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCAAGACTTCGCGGCAGGCGGCGGTATCAGACTcgc                                                                                                         >  1:274752/1‑140 (MQ=255)
                                 caccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCAAGACTTCGCGGCAGGCGGCGGTATCAGACTcgc                                                                                                         >  1:274849/1‑140 (MQ=255)
                                     acTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGtt                                                                                                     <  2:412885/140‑1 (MQ=255)
                                        tcttcCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTtg                                                                                                  <  1:172965/140‑1 (MQ=255)
                                        tcttcCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTtg                                                                                                  <  1:172538/140‑1 (MQ=255)
                                        tcttcCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTtg                                                                                                  <  2:1090330/140‑1 (MQ=255)
                                        tcttcCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTtg                                                                                                  <  2:942769/140‑1 (MQ=255)
                                         cttcCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTtgt                                                                                                 <  2:629941/140‑1 (MQ=255)
                                          ttcCAGTGGCAAATAGTCATACGCCACCATATACGTCATTTCATAAAGCAAAACCATAGCCAGTGAGGAATCAGAAAGCATCCGCTTTGCTTTTTGCAATACTTCGCGGCTGGGGGGGGTATCAGACTCGCGTTTTtgtg                                                                                                <  2:344128/140‑1 (MQ=255)
                                           tcCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTgtgt                                                                                               >  2:335610/1‑140 (MQ=255)
                                              aGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATc                                                                                            >  1:327369/1‑140 (MQ=255)
                                               gTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATcc                                                                                           <  1:312641/140‑1 (MQ=255)
                                               gTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATcc                                                                                           <  1:977028/140‑1 (MQ=255)
                                                           cATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGccc                                                                               >  1:589857/1‑140 (MQ=255)
                                                            aTACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCg                                                                              >  1:866534/1‑140 (MQ=255)
                                                              aCGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGtt                                                                            >  2:175546/1‑140 (MQ=255)
                                                                  caccaTATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCa                                                                        >  2:22647/1‑140 (MQ=255)
                                                                   accaTATACGTCAGTTGATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGAGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCAt                                                                       <  2:1157075/140‑1 (MQ=255)
                                                                    ccaTATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATc                                                                      >  1:1095710/1‑140 (MQ=255)
                                                                    ccaTATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATc                                                                      >  1:153860/1‑140 (MQ=255)
                                                                    ccaTATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATc                                                                      >  1:786722/1‑140 (MQ=255)
                                                                    ccaTATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATc                                                                      >  1:154151/1‑140 (MQ=255)
                                                                    ccaTATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATc                                                                      >  1:52758/1‑140 (MQ=255)
                                                                          aCGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGa                                                                >  1:1004524/1‑140 (MQ=255)
                                                                            gTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGaaa                                                              >  1:235201/1‑140 (MQ=255)
                                                                            gTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGATTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGaaa                                                              >  1:201232/1‑140 (MQ=255)
                                                                              cAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCTCCTGAAACt                                                            >  2:1218240/1‑140 (MQ=255)
                                                                               aGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTc                                                           >  1:975454/1‑140 (MQ=255)
                                                                               aGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTc                                                           >  1:975076/1‑140 (MQ=255)
                                                                               aGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTc                                                           >  2:1078758/1‑140 (MQ=255)
                                                                               aGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTc                                                           >  1:770590/1‑140 (MQ=255)
                                                                               aGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAAGATGCCAGTCTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTATTGTGTATCCCAGGTAAAGCCCGATTCCATCACCTGAAACTc                                                           >  2:1047755/1‑140 (MQ=255)
                                                                                 ttCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCaa                                                         >  1:427144/1‑140 (MQ=255)
                                                                                 ttCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCaa                                                         >  2:189947/1‑140 (MQ=255)
                                                                                 ttCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCaa                                                         >  2:558745/1‑140 (MQ=255)
                                                                                 ttCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCaa                                                         >  1:18946/1‑140 (MQ=255)
                                                                                 ttCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCaa                                                         >  1:15902/1‑140 (MQ=255)
                                                                                 ttCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCaa                                                         >  1:957573/1‑140 (MQ=255)
                                                                                 ttCATCAAGCAAAAACATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGccc                                                                               >  1:354781/1‑118 (MQ=255)
                                                                                 ttCATCAAGCAAAAACATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGccc                                                                               <  2:354781/118‑1 (MQ=255)
                                                                                   catcaAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTTAAACTAAACg                                                       >  2:361862/1‑140 (MQ=255)
                                                                                              aCCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTc                                            >  2:290938/1‑140 (MQ=255)
                                                                                              aCCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTc                                            >  2:289289/1‑140 (MQ=255)
                                                                                                    tCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCcagcag                                      >  1:279597/1‑140 (MQ=255)
                                                                                                         tGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGc                                 >  1:342515/1‑140 (MQ=255)
                                                                                                           agtaaTCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCCCCGTTTTGTGTATCCCAGGTAAACCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGt                               <  2:1203000/137‑1 (MQ=255)
                                                                                                            ggAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGtt                              <  2:913189/140‑1 (MQ=255)
                                                                                                             gAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTc                             >  2:501930/1‑140 (MQ=255)
                                                                                                              aaTCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCg                            >  2:267851/1‑140 (MQ=255)
                                                                                                              aaTCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCg                            >  2:495988/1‑140 (MQ=255)
                                                                                                              aaTCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCg                            >  2:1077882/1‑140 (MQ=255)
                                                                                                                     aagcaTCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAg                     >  2:287767/1‑140 (MQ=255)
                                                                                                                          tCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAg                <  1:454312/140‑1 (MQ=255)
                                                                                                                            cGCTTTGCATGTTGCAAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGt              <  2:274849/140‑1 (MQ=255)
                                                                                                                            cGCTTTGCATGTTGCAAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGt              <  2:274752/140‑1 (MQ=255)
                                                                                                                              ctgtGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGc            <  2:1150947/137‑1 (MQ=255)
                                                                                                                              cTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGc            <  2:1150334/140‑1 (MQ=255)
                                                                                                                              cTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGc            <  2:1150857/140‑1 (MQ=255)
                                                                                                                              cTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGc            <  2:1150921/140‑1 (MQ=255)
                                                                                                                              cTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGc            <  2:1056049/140‑1 (MQ=255)
                                                                                                                              cTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCAATAGGCCAGGTGc            >  2:283406/1‑140 (MQ=255)
                                                                                                                              cTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTAACAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGc            <  2:727259/140‑1 (MQ=255)
                                                                                                                                   cATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCCGGTGCCTTta       >  2:474968/1‑140 (MQ=255)
                                                                                                                                        tGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGCCTTtattaat  >  1:333029/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTGCGTTCGCCATTAGGCCAGGTGCCTTTAATAAA  >  NC_000913/1327893‑1328168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑