Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,381,217 G→T 8.9% pseudogene (397/957 nt) ycjV → pseudogene; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,381,2170GT 8.9% 150.7 / 3.4 45pseudogene (397/957 nt)ycjVpseudogene; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base G (15/26);  new base T (2/2);  total (17/28)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ATCCGCATATGACGGTTTACGACAACATGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTGATGCCAAGCTGCGCGTGCAAATGCGCGCAGAGATC  >  NC_000913/1381083‑1381351
                                                                                                                                      |                                                                                                                                      
aTCCGCATATGACGGTTTACGACAACATGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGgcg                                                                                                                                   >  2:802420/1‑140 (MQ=255)
       tatGACGGTTTACGACAACATGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCcg                                                                                                                            >  2:186603/1‑140 (MQ=255)
       tatGACGGTTTACGACAACATGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCcg                                                                                                                            >  2:187024/1‑140 (MQ=255)
                         caTGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAg                                                                                                          <  2:319066/140‑1 (MQ=255)
                         caTGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAg                                                                                                          <  2:1147331/140‑1 (MQ=255)
                         caTGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAg                                                                                                          >  2:1061181/1‑140 (MQ=255)
                         caTGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAg                                                                                                          <  1:902757/140‑1 (MQ=255)
                         caTGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAg                                                                                                          <  1:902622/140‑1 (MQ=255)
                         caTGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAg                                                                                                          >  2:241918/1‑140 (MQ=255)
                         caTGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAg                                                                                                          >  2:241998/1‑140 (MQ=255)
                              cGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGgcg                                                                                                     >  2:855246/1‑140 (MQ=255)
                               gTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGgcgc                                                                                                    <  1:191801/140‑1 (MQ=255)
                                   ggTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGTGGGCGCTTTCCGGCGGGCa                                                                                                                     <  1:426815/119‑1 (MQ=255)
                                   ggTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGTGGGCGCTTTCCGGCGGGCa                                                                                                                     <  1:426542/119‑1 (MQ=255)
                                   ggTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGTGGGCGCTTTCCGGCGGGCa                                                                                                                     >  2:426815/1‑119 (MQ=255)
                                   ggTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACCAAAGCCGTGGGCGCTTTCCGGCGGGCa                                                                                                                     >  2:426542/1‑119 (MQ=255)
                                       tGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTgggcgg                                                                                            >  1:725768/1‑140 (MQ=255)
                                        gAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTgggcggg                                                                                           >  1:260554/1‑140 (MQ=255)
                                              gCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTg                                                                                     >  1:948745/1‑140 (MQ=255)
                                                  aaaaTCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTAcg                                                                                 <  2:171484/140‑1 (MQ=255)
                                                   aaaTCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTAcgc                                                                                >  2:849101/1‑140 (MQ=255)
                                                                    attgatGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGttttt                                                               <  2:930128/140‑1 (MQ=255)
                                                                    attgatGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGttttt                                                               <  2:930021/140‑1 (MQ=255)
                                                                      tgatgaGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTgggcgg                                                                                            >  1:564110/1‑109 (MQ=255)
                                                                      tgatgaGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTgggcgg                                                                                            <  2:564110/109‑1 (MQ=255)
                                                                                 tGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCg                                                  <  2:34472/140‑1 (MQ=255)
                                                                                 tGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCg                                                  <  1:1146487/140‑1 (MQ=255)
                                                                                 tGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCg                                                  <  1:1206410/140‑1 (MQ=255)
                                                                                       gggCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGctctct                                            <  1:198975/140‑1 (MQ=255)
                                                                                       gggCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGctctct                                            <  2:81193/140‑1 (MQ=255)
                                                                                       gggCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGctctct                                            <  2:81143/140‑1 (MQ=255)
                                                                                            gcgcAAATTCTCGGCCTGCGTGTGTACCTGAAACTTAAGCCGGGGGCGCTTTCGGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCt                                       <  2:258234/140‑1 (MQ=255)
                                                                                            gcgcAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCt                                       <  1:565180/140‑1 (MQ=255)
                                                                                            gcgcAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCt                                       <  2:517716/140‑1 (MQ=255)
                                                                                            gcgcAAATTCTCGGCCTGAGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCt                                       <  2:224979/140‑1 (MQ=255)
                                                                                              gcAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTg                                     <  2:464102/140‑1 (MQ=255)
                                                                                              gcAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTg                                     <  1:1106393/140‑1 (MQ=255)
                                                                                              gcAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTg                                     <  1:1106470/140‑1 (MQ=255)
                                                                                              gcAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTg                                     <  2:464105/140‑1 (MQ=255)
                                                                                                      tcGGCCAGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTGATGCCAAg                             <  2:418081/140‑1 (MQ=255)
                                                                                                              cGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTGATGCCAAGCTGCGCGt                     >  1:1084065/1‑140 (MQ=255)
                                                                                                                     aCCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTGATGCCAAGCTGCGCGTGCAAATg              <  2:43806/140‑1 (MQ=255)
                                                                                                                           aaCGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTGATGCCAAGCTGCGCGTGCAAATGCGCGCa        <  1:802420/140‑1 (MQ=255)
                                                                                                                              gTAAGCCGGGGGCTCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTGTTAATGGATGAACCGCTCTCTATCCTTGATGCCAAGCTGCGCGTGCGAATGCGCGCagag     >  1:768409/1‑140 (MQ=255)
                                                                                                                                 aGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTGATGCCAAGCTGCGCGTGCAAATGCGCGCAGAGATc  >  1:203008/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                      
ATCCGCATATGACGGTTTACGACAACATGGCGTTTGGTCTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAAATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGTCAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAACCGCTCTCTAACCTTGATGCCAAGCTGCGCGTGCAAATGCGCGCAGAGATC  >  NC_000913/1381083‑1381351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑