Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,527,358 A→G 6.0% intergenic (+206/‑544) yncH → / → rhsE IPR020099 family protein/pseudogene, Rhs family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,527,3580AG 6.0% 219.1 / 3.9 67intergenic (+206/‑544)yncH/rhsEIPR020099 family protein/pseudogene, Rhs family
Reads supporting (aligned to +/- strand):  ref base A (40/23);  new base G (2/2);  total (42/25)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

AGTCAGAGCTAAAACAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCTTATTA  >  NC_000913/1527219‑1527495
                                                                                                                                           |                                                                                                                                         
aGTCAGAGCTAAAACAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGa                                                                                                                                           <  2:122938/140‑1 (MQ=255)
          aaaaCAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCAtt                                                                                                                                 >  1:1069527/1‑140 (MQ=255)
          aaaaCAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCAtt                                                                                                                                 >  1:444526/1‑140 (MQ=255)
          aaaaCAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCAtt                                                                                                                                 >  1:793839/1‑140 (MQ=255)
            aaCAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCAtttt                                                                                                                               <  1:188310/140‑1 (MQ=255)
            aaCAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCAtttt                                                                                                                               <  1:186585/140‑1 (MQ=255)
                 cAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGac                                                                                                                          >  1:795647/1‑139 (MQ=255)
                 cAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGaa                                                                                                                          >  1:796010/1‑140 (MQ=255)
                 cAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGaa                                                                                                                          >  1:902551/1‑140 (MQ=255)
                  aaTTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAAc                                                                                                                         >  1:738742/1‑140 (MQ=255)
                       aTTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGc                                                                                                                    <  1:931499/140‑1 (MQ=255)
                       aTTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGc                                                                                                                    <  1:364133/140‑1 (MQ=255)
                       aTTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGc                                                                                                                    <  1:364153/140‑1 (MQ=255)
                          aataaACATGTTGATATATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATc                                                                                                                 <  1:624222/140‑1 (MQ=255)
                           ataaACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCa                                                                                                                >  1:789262/1‑140 (MQ=255)
                              aaCATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGt                                                                                                             <  2:975308/140‑1 (MQ=255)
                                  tGTTGATTTATTCAAGAAAATGGATGTATTTTATATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCAGTTTGGGAACCATGCATCTGGTCctc                                                                                                         >  2:1232597/1‑140 (MQ=255)
                                  tGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTActc                                                                                                         >  2:1232672/1‑140 (MQ=255)
                                      gATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTAc                                                                                                     >  1:895666/1‑140 (MQ=255)
                                         ttATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCAc                                                                                                  <  1:719768/140‑1 (MQ=255)
                                         ttATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCAc                                                                                                  <  1:1091191/140‑1 (MQ=255)
                                                      tGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGatca                                                                                     >  2:404763/1‑140 (MQ=255)
                                                        gATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGatcatc                                                                                   <  1:749362/140‑1 (MQ=255)
                                                        gATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGatcatc                                                                                   <  1:749224/140‑1 (MQ=255)
                                                             aTTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAATATCATCCGGCa                                                                              <  2:1110971/140‑1 (MQ=255)
                                                                ttAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCa                                                                           <  1:1154567/140‑1 (MQ=255)
                                                                ttAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCa                                                                           <  1:296566/140‑1 (MQ=255)
                                                                ttAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCa                                                                           <  1:294327/140‑1 (MQ=255)
                                                                        tgcttCTTAATATAACCAAATCAATAGAGTATCAAGTGAGACCTACACAGATTTGGCGGTGCAGGGGAAAACACCAGTTTGGGAACAATGCATCAGGTACTCTTACCACGGAAGCAACTTCATCCGGCACCAggtggtgg                                                                   >  2:879674/4‑140 (MQ=255)
                                                                            tCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCa                                                               <  1:158764/140‑1 (MQ=255)
                                                                                      aCCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGa                                                     >  2:745349/1‑140 (MQ=255)
                                                                                      aCCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGa                                                     >  2:745670/1‑140 (MQ=255)
                                                                                          aaTCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGGAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCa                                                                                                   <  2:274111/90‑1 (MQ=255)
                                                                                          aaTCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGGAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCa                                                                                                   >  1:274111/1‑90 (MQ=255)
                                                                                          aaTCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGGAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCa                                                                                                   <  2:274091/90‑1 (MQ=255)
                                                                                          aaTCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGGAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCa                                                                                                   >  1:274091/1‑90 (MQ=255)
                                                                                          aaTCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATg                                                 >  1:327772/1‑140 (MQ=255)
                                                                                            tCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAATCACCATTTTGGGAAAAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGt                                               >  2:810495/1‑140 (MQ=255)
                                                                                            tCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAATTGGt                                               >  1:859313/1‑140 (MQ=255)
                                                                                            tCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGt                                               >  2:665645/1‑140 (MQ=255)
                                                                                                tAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTcccc                                           >  2:856790/1‑140 (MQ=255)
                                                                                                tAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTcccc                                           >  2:599064/1‑140 (MQ=255)
                                                                                                tAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTcccc                                           >  2:997763/1‑140 (MQ=255)
                                                                                                  gATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTc                                         >  2:1150951/1‑140 (MQ=255)
                                                                                                              agagCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTc                             >  1:184389/1‑140 (MQ=255)
                                                                                                                 gCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCatt                          <  1:139380/140‑1 (MQ=255)
                                                                                                                 gCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCatt                          <  1:665124/140‑1 (MQ=255)
                                                                                                                          tattGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTAt                 >  1:851876/1‑140 (MQ=255)
                                                                                                                          tattGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTAt                 >  2:132528/1‑140 (MQ=255)
                                                                                                                          tattGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTAt                 >  1:707383/1‑140 (MQ=255)
                                                                                                                          tattGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCAATGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTAt                 >  2:1181261/1‑140 (MQ=255)
                                                                                                                          tattGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCAATGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTAt                 >  2:1181287/1‑140 (MQ=255)
                                                                                                                               gAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTAt            >  1:1075105/1‑140 (MQ=255)
                                                                                                                                  gggCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGc                                                                                            >  1:1058603/1‑57 (MQ=255)
                                                                                                                                  gggCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGc                                                                                            <  2:1058603/57‑1 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGACATGGTCCCCTCGGGTGAGTTGTCATTATGTTCTATGCTATTTGCt       >  2:1135125/1‑140 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGTGGGAGTTGTCATTATTTTCTATGCTATTTGCt       >  2:1119002/1‑140 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCt       >  1:784641/1‑140 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCt       >  2:654054/1‑140 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCt       >  1:786754/1‑140 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCt       >  1:82725/1‑140 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCt       >  2:997308/1‑140 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGAACGCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGAGCTTTGGAAATGGTCCCCTCGGGGGAGATGTCAGTATTTTCTATGCTATTTGCt       >  2:1135126/1‑140 (MQ=255)
                                                                                                                                    gCAGGGGAAAACACCATTTTGCGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGTTGGTGCAGTGCGTGGGAAATGGTCCCCTCGGGGGAGTTGTCAATATTTACTATGCTAGTTGCt       >  2:351616/1‑140 (MQ=255)
                                                                                                                                      aGGGGAAATCACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCtta     <  1:63127/140‑1 (MQ=255)
                                                                                                                                      aGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCtta     <  1:312133/140‑1 (MQ=255)
                                                                                                                                      aGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCtta     <  1:1161955/140‑1 (MQ=255)
                                                                                                                                         ggAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCttatta  <  1:969902/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
AGTCAGAGCTAAAACAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTTGCTTATTA  >  NC_000913/1527219‑1527495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑