Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,037,789 C→A 9.5% D122Y (GAT→TAT)  yedV ← putative sensory kinase in two‑component regulatory system with YedW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,037,7890CA 9.5% 144.2 / 3.6 42D122Y (GAT→TAT) yedVputative sensory kinase in two‑component regulatory system with YedW
Reads supporting (aligned to +/- strand):  ref base C (22/16);  new base A (2/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

GCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCAAGATATC  >  NC_000913/2037650‑2037921
                                                                                                                                           |                                                                                                                                    
gCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATc                                                                                                                                      <  2:748472/140‑1 (MQ=255)
gCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATc                                                                                                                                      <  2:747780/140‑1 (MQ=255)
               ataATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATAAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCt                                                                                                                       >  2:320319/1‑140 (MQ=255)
                        ttAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGcc                                                                                                              >  2:122585/1‑140 (MQ=255)
                        ttAATTTTATACTGTTCAAGCAAGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAAAGATGGCGAAACTACATCAATATTAATACGAAAAGCATCAAACTCTGTATCATAAATAAAGCTACTGAAAATGcc                                                                                                              >  2:1170196/1‑140 (MQ=255)
                          aaTTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAg                                                                                                            <  1:844984/140‑1 (MQ=255)
                               tataCTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgcg                                                                                                       <  2:649117/140‑1 (MQ=255)
                                    cgTTCAAGCATGTTATGACAGGCAGAAGCCAAATTAGCCACAGTAACCGATAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGAt                                                                                                  <  1:1231957/139‑1 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  1:876803/1‑140 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  1:697674/1‑140 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  1:584097/1‑140 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  1:584064/1‑140 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  1:807047/1‑140 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  1:191289/1‑140 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  1:1164476/1‑140 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATAATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  2:122610/1‑140 (MQ=255)
                                      ttCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATAATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTg                                                                                                >  2:119239/1‑140 (MQ=255)
                                                ttATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGc                                                                                      >  2:2757/1‑140 (MQ=255)
                                                     tCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTa                                                                                 <  2:998530/140‑1 (MQ=255)
                                                               gCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTaa                                                                       >  2:240959/1‑140 (MQ=255)
                                                               gCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTaa                                                                       >  1:1161151/1‑140 (MQ=255)
                                                                      tAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCa                                                                <  2:262032/140‑1 (MQ=255)
                                                                      tAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCa                                                                <  2:262007/140‑1 (MQ=255)
                                                                      tAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCa                                                                <  2:239293/140‑1 (MQ=255)
                                                                       aGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCAt                                                               >  2:425312/1‑140 (MQ=255)
                                                                                         tgatgGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGt                                             >  1:851254/1‑140 (MQ=255)
                                                                                             ggCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAAc                                         >  1:1199352/1‑140 (MQ=255)
                                                                                             ggCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAAc                                         >  1:490713/1‑140 (MQ=255)
                                                                                             ggCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAAc                                         >  1:386875/1‑140 (MQ=255)
                                                                                             ggCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTGCTGTAAATGCCAGCTGCGCTGATTGTCTCAATAGCAGGTATGTTATTTAACATGCCATCACTTACATTTGTCCGGTTAAc                                         >  2:666034/1‑140 (MQ=255)
                                                                                                    cTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTa                                  <  1:1031170/140‑1 (MQ=255)
                                                                                                    cTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTa                                  <  2:662942/140‑1 (MQ=255)
                                                                                                        atcaATATTAATTCGTAAAGCATCTATCTCTGTAACATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGa                              <  2:484723/140‑1 (MQ=255)
                                                                                                                       tAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGaa               >  1:238307/1‑140 (MQ=255)
                                                                                                                          aGCATCTATCTCTGTATAATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGaataa            <  1:122610/140‑1 (MQ=255)
                                                                                                                          aGCATCTATCTCTGTATAATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGaataa            <  1:119239/140‑1 (MQ=255)
                                                                                                                              tctatctCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCaa        >  1:680417/1‑140 (MQ=255)
                                                                                                                              tctatctCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCaa        >  1:16692/1‑140 (MQ=255)
                                                                                                                                    tctGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCAAGATATc  <  1:407771/140‑1 (MQ=255)
                                                                                                                                    tctGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCAAGATATc  <  1:723716/140‑1 (MQ=255)
                                                                                                                                    tctGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCAAGATATc  <  1:991222/140‑1 (MQ=255)
                                                                                                                                    tctGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCAAGATATc  <  1:723725/140‑1 (MQ=255)
                                                                                                                                    tctGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCAAGATATc  <  1:874241/140‑1 (MQ=255)
                                                                                                                                    tctGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCAAGATATc  <  1:874171/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                    
GCGACAATGCAAATTATAATGCTATTAATTTTATACTGTTCAAGCATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTCACTAGCAGGTATGTTATTTAACATGCCATCACTGACATTTGTCCGGTTAACAATTTTATTGATGCTATCACCATGAATAATCAAGATATC  >  NC_000913/2037650‑2037921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: