Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,088,950	G→T	5.3%	S60Y (TCT→TAT)	yeeZ ←	putative epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,088,950	0	G	T	5.3%	260.7 / 2.4	76	S60Y (TCT→TAT)	yeeZ	putative epimerase
Reads supporting (aligned to +/- strand):  ref base G (51/21);  new base T (2/2);  total (53/23)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

GAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCAT  >  NC_000913/2088816‑2089089                                                                                                                                       |                                                                                                                                            gAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCg                                                                                                                                        <  1:623701/140‑1 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCACACTAGCTc                                                                                                                             >  2:1033952/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  2:1034089/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  1:1042901/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  1:1060667/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  2:988215/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  2:1112976/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  2:1182605/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  2:348297/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  2:5488/1‑140 (MQ=255)            gCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTc                                                                                                                             >  2:5514/1‑140 (MQ=255)                gcgcgcTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGc                                                                                                                         <  1:1033123/140‑1 (MQ=255)                 cgcgcTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCt                                                                                                                        >  1:224589/1‑140 (MQ=255)                 cgcgcTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGAGCGTCAGAATCGCAAACAAGCTCAGGCt                                                                                                                        >  2:17605/1‑140 (MQ=255)                      tATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATg                                                                                                                   >  1:340748/1‑140 (MQ=255)                      tATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATg                                                                                                                   >  1:983840/1‑140 (MQ=255)                        tCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCg                                                                                                                 <  2:827590/140‑1 (MQ=255)                        tCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCg                                                                                                                 <  1:721493/140‑1 (MQ=255)                            cTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGc                                                                                                             <  1:599308/140‑1 (MQ=255)                               aCTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGa                                                                                                          >  1:830046/1‑140 (MQ=255)                                ctctTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGAt                                                                                                         >  2:1048271/1‑140 (MQ=255)                                  ctTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAg                                                                                                       >  2:1003287/1‑140 (MQ=255)                                    tGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCt                                                                                                     >  2:822981/1‑140 (MQ=255)                                    tGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCt                                                                                                     >  2:1167019/1‑140 (MQ=255)                                    tGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCt                                                                                                     >  2:641377/1‑140 (MQ=255)                                           cTTGTAAATAGTACTCATCGGCGGGGCCGCTTCGTCGTGGCGGTTGCGTAATGACCAGCGCATCGGCTTCCATGTGGGCATCCAGATCGTCAGATTCGCAATCTAGCTGAGGCTCCATGCGTAGCAGTTTGCTATCAATg                                                                                              >  2:1125748/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGTCGTGCCGGAAGCGTAATGTCCAGCGCATCGGCATCCATCAGGGCTTCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:1125781/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCATAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:225606/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCATAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:225634/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:1113427/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:714495/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:644617/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:209917/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:352077/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:40810/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  1:327566/1‑140 (MQ=255)                                           cTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAAACGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATg                                                                                              >  2:1058365/1‑140 (MQ=255)                                              gTAAATCGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCa                                                                                           <  2:818068/140‑1 (MQ=255)                                              gTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCACTGCCa                                                                                           <  2:818045/140‑1 (MQ=255)                                                      aaCTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCg                                                                                   >  2:884157/1‑140 (MQ=255)                                                      aaCTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCg                                                                                   >  2:884152/1‑140 (MQ=255)                                                      aaCTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCg                                                                                   >  1:232529/1‑140 (MQ=255)                                                      aaCTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCg                                                                                   >  1:617720/1‑140 (MQ=255)                                                         tCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGc                                                                                <  2:494703/140‑1 (MQ=255)                                                           aTCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCg                                                                              >  2:693119/1‑140 (MQ=255)                                                           aTCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCg                                                                              >  1:361142/1‑140 (MQ=255)                                                           aTCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCg                                                                              >  1:361320/1‑140 (MQ=255)                                                              ggcGGGGCCGCTACGACGTGCCGGAAGCGTATGGACCAGCGCATCGGCTTCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGGTCAGGCTCCATGCGATGCAGATAGATTTCAATGCCACTCATTCGGGCCGCtt                                                                           <  2:343057/138‑1 (MQ=255)                                                                  gggCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGac                                                                       <  2:999884/140‑1 (MQ=255)                                                                   ggCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCATAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGaca                                                                      <  1:225634/140‑1 (MQ=255)                                                                   ggCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCATAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGaca                                                                      <  1:225606/140‑1 (MQ=255)                                                                     ccGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACAcc                                                                    >  1:756210/1‑140 (MQ=255)                                                                     ccGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACAcc                                                                    >  1:756360/1‑140 (MQ=255)                                                                     ccGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACAcc                                                                    >  1:85936/1‑140 (MQ=255)                                                                             acgTGCCGGAAGCGTAATGTCCAGCGCATCGGCATCCATCAGGGCATCCATATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGTAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTtgtg                                                            <  1:785904/140‑1 (MQ=255)                                                                             acgTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTtgtg                                                            <  2:342906/140‑1 (MQ=255)                                                                             acgTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTtgtg                                                            <  1:932326/140‑1 (MQ=255)                                                                             acgTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTtgtg                                                            <  1:1193379/140‑1 (MQ=255)                                                                                tGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTgg                                                         >  1:375482/1‑140 (MQ=255)                                                                                tGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTgg                                                         >  1:150712/1‑140 (MQ=255)                                                                                  ccGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGtt                                                       >  1:968524/1‑140 (MQ=255)                                                                                  ccGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGtt                                                       >  1:1122704/1‑140 (MQ=255)                                                                                  ccGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGtt                                                       >  1:406500/1‑140 (MQ=255)                                                                                   cGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACAATCTTGTGTGGttt                                                      <  2:860768/140‑1 (MQ=255)                                                                                          gTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTccc                                               <  1:1005208/140‑1 (MQ=255)                                                                                           tAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCg                                              >  2:562963/1‑140 (MQ=255)                                                                                           tAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCg                                              >  2:560294/1‑140 (MQ=255)                                                                                           tAATGACCAGCGCATCGGCATCCATCAGGGAATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAAAGCCACTCATTCGGGCCGATTCGACACCATCTTGTGTGGTTTTACTCCCg                                              >  2:574347/1‑140 (MQ=255)                                                                                             aTGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCTTTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGt                                            <  1:570196/140‑1 (MQ=255)                                                                                                   aGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTg                                      >  2:916154/1‑140 (MQ=255)                                                                                                    gcgcATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGc                                     <  1:149662/140‑1 (MQ=255)                                                                                                               tccatcAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCgcgc                          <  2:756210/140‑1 (MQ=255)                                                                                                               tccatcAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCgcgc                          <  2:756360/140‑1 (MQ=255)                                                                                                               tccatcAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCgcgc                          <  2:85936/140‑1 (MQ=255)                                                                                                                    cAGGGAAACCAGATCGTCAGAATCGCAAACAAGCTCAGGCTCCATGCGAAGCATATAGCTATCAATGACACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGaaa                     >  2:115166/1‑140 (MQ=255)                                                                                                                                 tCGACAGAAACGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTATACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAg        >  1:1184014/1‑140 (MQ=255)                                                                                                                                       gAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCAt  <  2:1147588/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                            GAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCAT  >  NC_000913/2088816‑2089089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑