Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,089,011	C→A	10.5%	A40S (GCC→TCC)	yeeZ ←	putative epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,089,011	0	C	A	10.5%	181.2 / 7.4	57	A40S (GCC→TCC)	yeeZ	putative epimerase
Reads supporting (aligned to +/- strand):  ref base C (21/30);  new base A (3/3);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATA  >  NC_000913/2088872‑2089144                                                                                                                                            |                                                                                                                                      tCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGc                                                                                                                                       <  2:494703/140‑1 (MQ=255)   aTCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCg                                                                                                                                     >  1:361320/1‑140 (MQ=255)   aTCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCg                                                                                                                                     >  1:361142/1‑140 (MQ=255)   aTCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCg                                                                                                                                     >  2:693119/1‑140 (MQ=255)      ggcGGGGCCGCTACGACGTGCCGGAAGCGTATGGACCAGCGCATCGGCTTCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGGTCAGGCTCCATGCGATGCAGATAGATTTCAATGCCACTCATTCGGGCCGCtt                                                                                                                                  <  2:343057/138‑1 (MQ=255)          gggCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGac                                                                                                                              <  2:999884/140‑1 (MQ=255)           ggCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCATAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGaca                                                                                                                             <  1:225606/140‑1 (MQ=255)           ggCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCATAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGaca                                                                                                                             <  1:225634/140‑1 (MQ=255)             ccGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACAcc                                                                                                                           >  1:85936/1‑140 (MQ=255)             ccGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACAcc                                                                                                                           >  1:756210/1‑140 (MQ=255)             ccGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACAcc                                                                                                                           >  1:756360/1‑140 (MQ=255)                     acgTGCCGGAAGCGTAATGTCCAGCGCATCGGCATCCATCAGGGCATCCATATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGTAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTtgtg                                                                                                                   <  1:785904/140‑1 (MQ=255)                     acgTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTtgtg                                                                                                                   <  1:1193379/140‑1 (MQ=255)                     acgTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTtgtg                                                                                                                   <  2:342906/140‑1 (MQ=255)                     acgTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTtgtg                                                                                                                   <  1:932326/140‑1 (MQ=255)                        tGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTgg                                                                                                                >  1:150712/1‑140 (MQ=255)                        tGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTgg                                                                                                                >  1:375482/1‑140 (MQ=255)                          ccGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGtt                                                                                                              >  1:1122704/1‑140 (MQ=255)                          ccGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGtt                                                                                                              >  1:406500/1‑140 (MQ=255)                          ccGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGtt                                                                                                              >  1:968524/1‑140 (MQ=255)                           cGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACAATCTTGTGTGGttt                                                                                                             <  2:860768/140‑1 (MQ=255)                                  gTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTccc                                                                                                      <  1:1005208/140‑1 (MQ=255)                                   tAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCg                                                                                                     >  2:562963/1‑140 (MQ=255)                                   tAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCg                                                                                                     >  2:560294/1‑140 (MQ=255)                                   tAATGACCAGCGCATCGGCATCCATCAGGGAATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAAAGCCACTCATTCGGGCCGATTCGACACCATCTTGTGTGGTTTTACTCCCg                                                                                                     >  2:574347/1‑140 (MQ=255)                                     aTGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCTTTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGt                                                                                                   <  1:570196/140‑1 (MQ=255)                                           aGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTg                                                                                             >  2:916154/1‑140 (MQ=255)                                            gcgcATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGc                                                                                            <  1:149662/140‑1 (MQ=255)                                                       tccatcAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCgcgc                                                                                 <  2:756210/140‑1 (MQ=255)                                                       tccatcAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCgcgc                                                                                 <  2:756360/140‑1 (MQ=255)                                                       tccatcAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGACGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCgcgc                                                                                 <  2:85936/140‑1 (MQ=255)                                                            cAGGGAAACCAGATCGTCAGAATCGCAAACAAGCTCAGGCTCCATGCGAAGCATATAGCTATCAATGACACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGaaa                                                                            >  2:115166/1‑140 (MQ=255)                                                                         tCGACAGAAACGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTATACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAg                                                               >  1:1184014/1‑140 (MQ=255)                                                                               gAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCAt                                                         <  2:1147588/140‑1 (MQ=255)                                                                                      aaaCTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAac                                                  <  1:936525/140‑1 (MQ=255)                                                                                      aaaCTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAac                                                  <  1:936402/140‑1 (MQ=255)                                                                                      aaaCTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAac                                                  <  1:936357/140‑1 (MQ=255)                                                                                         cTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAaccac                                               <  2:427669/140‑1 (MQ=255)                                                                                         cTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCACCAGCGGCATGCCTAaccac                                               <  2:66839/140‑1 (MQ=255)                                                                                              tCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTaa                                          <  2:968524/140‑1 (MQ=255)                                                                                              tCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTaa                                          <  2:406500/140‑1 (MQ=255)                                                                                              tCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTaa                                          <  2:1122704/140‑1 (MQ=255)                                                                                                        tGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAAtt                                >  1:536827/1‑140 (MQ=255)                                                                                                            aaGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGa                            >  2:170887/1‑140 (MQ=255)                                                                                                             aGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGAc                           <  2:1133887/140‑1 (MQ=255)                                                                                                               cAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCt                         <  2:180811/140‑1 (MQ=255)                                                                                                               cAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCt                         <  2:180796/140‑1 (MQ=255)                                                                                                                aGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCtt                        >  1:894933/1‑140 (MQ=255)                                                                                                                 gATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCttt                       <  1:1003287/140‑1 (MQ=255)                                                                                                                      cTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCAt                  <  1:58278/140‑1 (MQ=255)                                                                                                                         tCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCg               >  1:266238/1‑140 (MQ=255)                                                                                                                            aTGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTa            >  2:550686/1‑140 (MQ=255)                                                                                                                            aTGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTa            <  2:536827/140‑1 (MQ=255)                                                                                                                               ccACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTAtct         <  2:327566/140‑1 (MQ=255)                                                                                                                                 aCTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTcc       >  2:46466/1‑140 (MQ=255)                                                                                                                                   tCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTg     >  1:1090222/1‑140 (MQ=255)                                                                                                                                    cATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGa    <  1:492040/140‑1 (MQ=255)                                                                                                                                      ttCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATa  <  2:222718/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                      TCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGCCACTCATTCGGGCCGCTTCGACACCATCTTGTGTGGTTTTACTCCCGGTGACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATA  >  NC_000913/2088872‑2089144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑