Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,192,944 C→A 8.0% intergenic (‑148/+115) yehE ← / ← mrp DUF2574 family protein/antiporter inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,192,9440CA 8.0% 225.4 / 7.8 75intergenic (‑148/+115)yehE/mrpDUF2574 family protein/antiporter inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (20/49);  new base A (3/3);  total (23/52)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

CTGTAGTAAAGGTTATGCCTGGATAGAATGAGTGCATAACAAACTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATAGG  >  NC_000913/2192809‑2193056
                                                                                                                                       |                                                                                                                
cTGTAGTAAAGGTTATGCCTGGATAGAATGAGTGCATAACAAACTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCa                                                                                                              >  1:160956/1‑140 (MQ=255)
   tagtaAAGGTTATGCCTGGATAGAATGAGTGCATAACAAACTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGaa                                                                                                           >  1:777546/1‑140 (MQ=255)
            ttATGCCTGGATAGAATGAGTGCATAACAAACTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCCGAATATTCGCAg                                                                                                  <  2:482639/140‑1 (MQ=255)
             taatccTGGATAGAATGAGTTCATAAAAAAAAATAGCTGTACATCCAATACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTACGCAGAATATTCGCAGt                                                                                                 >  2:753562/5‑140 (MQ=255)
             tATGCCTGGATAGAATGATTGCATAAAAAAATAAAGCTGTAAATCAAATACACAGCCACGAAGGATGATAATGAATCAATGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTACGCAGAATATTCGCAGt                                                                                                 >  2:749982/1‑140 (MQ=255)
             tATGCCTGGATAGAATGAGTGCATAACAAACTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGt                                                                                                 >  1:21465/1‑140 (MQ=255)
             tATGCCTGGATAGAATGAGTGCATAAAAAACTAAAGCTGTAAATAAACTAAACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTACGCAGAATATTCGCAGt                                                                                                 >  2:638970/1‑140 (MQ=255)
                     gATAGAATGAGTGCATAACAAACTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATa                                                                                         >  1:281173/1‑140 (MQ=255)
                      aTAGAATGAGTGCATAACAAACTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATaa                                                                                        <  1:608162/140‑1 (MQ=255)
                                        aaaCTATCGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:125872/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCGACTACACAGCCTCGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:83041/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTCCACAGCCACGAAGGATGATAATGTAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:1181555/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCTCGAAGGATGATTATGAAGAATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:955613/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGATGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGATGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:814743/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGTTGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:924283/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGTAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:1225108/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:255072/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:247454/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:21465/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:341970/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:384848/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:125917/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:441953/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:465071/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:578213/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:470535/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:888245/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  1:475375/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:1030063/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:1004261/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:735249/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:735177/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATAAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:125529/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGAATGATCAATCGACTTTGTAGTGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:1159227/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCATGTATGAACAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAACTGTTCATTAATGCGGCa                                                                      <  2:160956/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAAAGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:568178/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACGACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCATTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:674720/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATCCACAACACAGCCACGAAGGATGATAATGAAGCATAGCCTGTATGAACAATCGACTTTGTAGAGTTACGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:888113/140‑1 (MQ=255)
                                        aaaCTATAGCTGTACATACACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:689401/140‑1 (MQ=255)
                                          aCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCaaa                                                                    <  2:773157/140‑1 (MQ=255)
                                          aCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCaaa                                                                    >  2:501590/1‑140 (MQ=255)
                                          aCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCaaa                                                                    <  1:240375/140‑1 (MQ=255)
                                          aCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCaaa                                                                    <  1:240342/140‑1 (MQ=255)
                                          aCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:643087/138‑1 (MQ=255)
                                          aCTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      >  1:643087/1‑138 (MQ=255)
                                                 cTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      >  1:453812/1‑131 (MQ=255)
                                                 cTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCa                                                                      <  2:453812/131‑1 (MQ=255)
                                                  tGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATa                                                            >  2:175135/1‑140 (MQ=255)
                                                    tACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATaca                                                          >  1:1190445/1‑140 (MQ=255)
                                                      cATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGTAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGCCCAAAACTAATACAcc                                                        >  1:243317/1‑140 (MQ=255)
                                                         ccACTACACAGCCACGAAGGATGATAATGAAGCATGGCCTGTATGATCAATCGACTTTGGAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCTGTATTAAAGATGTGTTCTTTAATGCGGCAAAAACGAATACACCGCa                                                     >  2:1206779/1‑140 (MQ=255)
                                                           aCTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAATAACTAATACACCGCATc                                                   >  1:1225595/1‑140 (MQ=255)
                                                           aCTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATc                                                   >  1:1225501/1‑140 (MQ=255)
                                                                         aaGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTTAATAATTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAAAATctct                                     >  2:282422/1‑140 (MQ=255)
                                                                            gatgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCg                                  <  2:965393/140‑1 (MQ=255)
                                                                             atgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg                                 <  2:713572/140‑1 (MQ=255)
                                                                             atgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg                                 <  2:801636/140‑1 (MQ=255)
                                                                             atgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg                                 <  2:75220/140‑1 (MQ=255)
                                                                             atgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg                                 <  2:295218/140‑1 (MQ=255)
                                                                             atgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg                                 <  2:1034889/140‑1 (MQ=255)
                                                                             atgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg                                 <  2:1064241/140‑1 (MQ=255)
                                                                             atgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg                                 <  2:117590/140‑1 (MQ=255)
                                                                             atgatAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg                                 <  2:1190445/140‑1 (MQ=255)
                                                                              tgatAAAGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGgt                                >  1:247147/1‑140 (MQ=255)
                                                                                       aGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATgaaga                       <  1:903247/140‑1 (MQ=255)
                                                                                       aGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATgaaga                       <  1:925890/140‑1 (MQ=255)
                                                                                       aGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATgaaga                       <  1:103860/140‑1 (MQ=255)
                                                                                                tGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGa              >  2:946851/1‑140 (MQ=255)
                                                                                                 gTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTACGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGaa             <  1:753562/140‑1 (MQ=255)
                                                                                                 gTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTACGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGaa             <  1:638970/140‑1 (MQ=255)
                                                                                                 gTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTACGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGaa             <  1:749982/140‑1 (MQ=255)
                                                                                                          aTCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATa    >  2:491338/1‑140 (MQ=255)
                                                                                                          aTCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATa    >  2:491250/1‑140 (MQ=255)
                                                                                                            cGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATAgg  >  1:506517/1‑140 (MQ=255)
                                                                                                            cGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATAgg  >  1:763104/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                
CTGTAGTAAAGGTTATGCCTGGATAGAATGAGTGCATAACAAACTATAGCTGTACATCCACTACACAGCCACGAAGGATGATAATGAAGCATTGCCTGTATGATCAATCGACTTTGTAGAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATAGG  >  NC_000913/2192809‑2193056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑