| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,581,618 | G→A | 7.5% | V661V (GTG→GTA) | tktB → | transketolase 2, thiamine triphosphate‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,581,618 | 0 | G | A | 7.5% | 174.7 / 4.5 | 53 | V661V (GTG→GTA) | tktB | transketolase 2, thiamine triphosphate‑binding |
| Reads supporting (aligned to +/- strand): ref base G (22/27); new base A (2/2); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
ATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGG > NC_000913/2581482‑2581757 | aTTACTGGTACAAGTATGTTGGTCTGAACGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGGTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAACGCGCATAAGGTGCTg < 2:1084473/140‑1 (MQ=255)aTTACTGCTACAACTATGTCGGTCTGCAAGGGGCAATTGTCGGCATGACGGGTCACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCATTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTg < 2:816606/140‑1 (MQ=255) ttACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTgg > 1:364579/1‑140 (MQ=255) ttACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGAGCTgg > 1:364426/1‑140 (MQ=255) gTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGa > 1:689143/1‑140 (MQ=255) gTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGa > 1:1061016/1‑140 (MQ=255) gTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGa > 1:280048/1‑140 (MQ=255) gTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTg > 1:14747/1‑140 (MQ=255) ttGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGa > 2:86551/1‑140 (MQ=255) tGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGAt < 1:607748/140‑1 (MQ=255) cTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtg > 2:266384/1‑140 (MQ=255) tGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtga < 1:1182033/140‑1 (MQ=255) tGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtga < 1:322932/140‑1 (MQ=255) tGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtga < 1:323083/140‑1 (MQ=255) aGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtgattgc < 1:569889/140‑1 (MQ=255) ggCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGATAATATTGTGGCAAAAGCGCATAAGGTACTGGGAGTGAAAGGTGCCTGATGGtgattgccgt > 2:788426/1‑139 (MQ=255) ggCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGATAATATTGTGGCAAAAGCGCATAAGGTACTGGGAGTGAAAGGTGCCTGATGGtgattgccgt > 2:788796/1‑139 (MQ=255) ggCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtgattgccgg > 2:448091/1‑140 (MQ=255) gCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtgattgccgga > 1:288544/1‑140 (MQ=255) ttGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtgattgccggatgct > 1:505119/1‑140 (MQ=255) ttGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGtgattgccggatgct > 1:505228/1‑140 (MQ=255) aTCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCTGATGCGACGCTGAcgcg > 2:537904/1‑140 (MQ=255) aTCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGAcgcg > 1:1060781/1‑140 (MQ=255) aTCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGAcgcg > 1:371728/1‑140 (MQ=255) cTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTa > 2:49786/1‑140 (MQ=255) cTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTa > 2:352499/1‑140 (MQ=255) tCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTAt < 1:385747/140‑1 (MQ=255) tCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTAt < 1:138534/140‑1 (MQ=255) tCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTAt < 2:14747/140‑1 (MQ=255) tCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTAt < 2:284864/140‑1 (MQ=255) accGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTAt < 2:58942/139‑1 (MQ=255) ggcggATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCg > 2:808965/1‑140 (MQ=255) ggcggATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCg > 2:809013/1‑140 (MQ=255) ggcggATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCg < 2:387511/140‑1 (MQ=255) tAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTAc > 1:959084/1‑140 (MQ=255) tGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGt < 1:1096104/140‑1 (MQ=255) ccGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTAt < 1:631121/140‑1 (MQ=255) ccGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTAt < 1:195081/140‑1 (MQ=255) ccGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTAt < 1:1002881/140‑1 (MQ=255) gCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGTAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACa < 2:1123604/140‑1 (MQ=255) cgccgAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCg < 2:160504/140‑1 (MQ=255) gagaATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCAt < 1:1007977/140‑1 (MQ=255) gaATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCAttt < 1:1008521/140‑1 (MQ=255) tgGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAAc < 2:112409/140‑1 (MQ=255) tgGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAAc < 2:1109672/140‑1 (MQ=255) tgGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAAc < 1:1125019/140‑1 (MQ=255) cAAAAGCGCATAAGGTGCTGGGAGTGAATGGTGCCTGATGGGGATTGCCGGATGCTGATTGCCGGATGCGACGCTGTCGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACggg < 2:339002/140‑1 (MQ=255) aaaGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGTTGGTGATAGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTg > 2:202876/1‑140 (MQ=255) gcATAAGGTACTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGTATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCca < 1:788796/140‑1 (MQ=255) gcATAAGGTACTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGTATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCca < 1:788426/140‑1 (MQ=255) aGGTGCTGGTAGTGAAAGGTGCCTGATGGTGATTGCCGGATTCTGATTGCCGTATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACg < 1:1169498/140‑1 (MQ=255) aGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACg < 1:1169415/140‑1 (MQ=255) ggTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGt > 2:964999/1‑140 (MQ=255) gCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTggg > 1:841539/1‑140 (MQ=255) gCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTAATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTggg > 1:441767/1‑140 (MQ=255) | ATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGG > NC_000913/2581482‑2581757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |