Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,595,174 G→T 5.5% S229* (TCA→TAA)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,595,1740GT 5.5% 260.1 / 2.6 73S229* (TCA→TAA) tmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (38/31);  new base T (2/2);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAG  >  NC_000913/2595040‑2595313
                                                                                                                                      |                                                                                                                                           
gATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCt                                                                                                                                        >  1:632672/1‑140 (MQ=255)
gATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCt                                                                                                                                        >  1:632016/1‑140 (MQ=255)
gATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCt                                                                                                                                        >  1:589071/1‑140 (MQ=255)
gATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACGTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCt                                                                                                                                        >  1:1206791/1‑140 (MQ=255)
  tGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGTCCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCttt                                                                                                                                      >  1:855995/1‑140 (MQ=255)
  tGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGTCCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCttt                                                                                                                                      >  1:856064/1‑140 (MQ=255)
  tGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCttt                                                                                                                                      >  1:878676/1‑140 (MQ=255)
      acaaTGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCg                                                                                                                                  <  2:395469/140‑1 (MQ=255)
      acaaTGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCg                                                                                                                                  >  1:713921/1‑140 (MQ=255)
      acaaTGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCg                                                                                                                                  >  1:616635/1‑140 (MQ=255)
      acaaTGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCg                                                                                                                                  <  1:212902/140‑1 (MQ=255)
      acaaTGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCg                                                                                                                                  <  1:212883/140‑1 (MQ=255)
      acaaTGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCg                                                                                                                                  >  1:1131847/1‑140 (MQ=255)
      acaaTGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCg                                                                                                                                  >  1:1132051/1‑140 (MQ=255)
       caaTGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGAgg                                                                                                                                 >  2:830013/1‑140 (MQ=255)
          tGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAACGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGgcg                                                                                                                              <  1:108442/140‑1 (MQ=255)
          tGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGgcg                                                                                                                              <  1:145925/140‑1 (MQ=255)
              gcAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGt                                                                                                                          <  1:1115528/140‑1 (MQ=255)
               cAGGTATGGCTGCGGCTTCAGCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCGTACAAGGCATCCGGCGCAAAAAAGCGAAACGACTCGCCCGCAAAGTGAGCCAGGTCTTCCGGTGACGCTTTGGCGGCCGGGGTg                                                                                                                         >  2:8007/1‑140 (MQ=255)
                     tGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTTACGCTTTTGCGGGCGCGGTGACAATc                                                                                                                   >  1:357096/1‑140 (MQ=255)
                     tGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTTACGCTTTTGCGGGCGCGGTGACAATc                                                                                                                   >  1:897480/1‑140 (MQ=255)
                     tGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATc                                                                                                                   >  1:417094/1‑140 (MQ=255)
                       gCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCGTTTGCGGGCGCGGTGACAATCGc                                                                                                                 >  2:631104/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACCTCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:682276/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:636916/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:637124/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:682272/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:1123777/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:1165039/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:93392/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:636909/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  2:505204/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  1:32333/1‑140 (MQ=255)
                           cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTg                                                                                                             >  1:32332/1‑140 (MQ=255)
                            ggCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGc                                                                                                            >  1:3991/1‑140 (MQ=255)
                            ggCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGc                                                                                                            >  1:39778/1‑140 (MQ=255)
                             gggacctCGACCACCTGCCAGTCGGGTTGCTCATCGCTGGCTAACACGGCATCCGGCGCACTATCGCGAAACTTCTCGCCCGCATCGTGTGGCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACATTCGCTCTGcc                                                                                                           <  2:791234/134‑1 (MQ=255)
                             gCTTCAACGACCACCAGCCAGTCGGATTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGcc                                                                                                           <  2:790961/140‑1 (MQ=255)
                                  aTCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACTAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCaa                                                                                                      <  2:154446/140‑1 (MQ=255)
                                  aTCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCaa                                                                                                      <  1:171995/140‑1 (MQ=255)
                                   tCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAAt                                                                                                     >  2:1183877/1‑140 (MQ=255)
                                    cGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATa                                                                                                    <  2:889477/140‑1 (MQ=255)
                                     gACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATAc                                                                                                   <  1:468913/140‑1 (MQ=255)
                                        caccaGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACgag                                                                                                >  2:593422/1‑140 (MQ=255)
                                        caccaGCCAGTCGGCGTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCACTACgag                                                                                                >  2:821098/1‑140 (MQ=255)
                                          ccagccagTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGaa                                                                                              <  2:351928/140‑1 (MQ=255)
                                          ccagccagTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGaa                                                                                              >  2:491650/1‑140 (MQ=255)
                                          ccagccagTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGaa                                                                                              >  2:433350/1‑140 (MQ=255)
                                                     gCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCACTAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGccc                                                                                   >  1:1194123/1‑140 (MQ=255)
                                                     gCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGccc                                                                                   >  1:1194281/1‑140 (MQ=255)
                                                                         cAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACcgc                                                               >  2:357358/1‑140 (MQ=255)
                                                                                  cGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCAcgcg                                                      <  2:638901/140‑1 (MQ=255)
                                                                                  cGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCAcgcg                                                      <  1:516862/140‑1 (MQ=255)
                                                                                  cGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCAcgcg                                                      <  1:719371/140‑1 (MQ=255)
                                                                                    gcgcAATAACGCGAACCTACTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACACTCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCa                                                    <  2:353654/140‑1 (MQ=255)
                                                                                            aaGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTAc                                            <  1:561802/140‑1 (MQ=255)
                                                                                                      tctcGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACg                                  <  2:656450/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTTACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:357096/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTTACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:897480/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCACTACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:854717/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:855729/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:829634/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  1:1066685/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:417094/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:1180548/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  1:584691/140‑1 (MQ=255)
                                                                                                                aaaTTGTGCCAGTACATCCGTTGACGCTTTAGCGGGCGCGGTGACAATAGCTCTGCCCGCCATACGAGAAAAGAGTTGCCCTGCCAACGCCGCCTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:660406/140‑1 (MQ=255)
                                                                                                                        ccAGTACATCCGTTGACACTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCAtt                >  2:703884/1‑140 (MQ=255)
                                                                                                                           gTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGATCGGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgc             <  2:1228413/140‑1 (MQ=255)
                                                                                                                           gTACAGCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTGGCCCTGCCAACGCCGCCGTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgc             <  2:1228406/140‑1 (MQ=255)
                                                                                                                            tACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        <  2:1064025/128‑1 (MQ=255)
                                                                                                                            tACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                        >  1:1064025/1‑128 (MQ=255)
                                                                                                                                  cGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTa      <  2:957382/140‑1 (MQ=255)
                                                                                                                                      gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAagag  >  1:1139949/1‑140 (MQ=255)
                                                                                                                                      gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAagag  >  1:1139913/1‑140 (MQ=255)
                                                                                                                                      gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAagag  >  1:1139896/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                           
GATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAG  >  NC_000913/2595040‑2595313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: