| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,767,845 | G→A | 7.3% | G46R (GGG→AGG) | yfjP → | CP4‑57 prophage; 50S ribosome‑binding GTPase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,767,845 | 0 | G | A | 7.3% | 186.1 / 3.8 | 55 | G46R (GGG→AGG) | yfjP | CP4‑57 prophage; 50S ribosome‑binding GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base G (24/27); new base A (2/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAACACTTTATGACCATCGTGGATCTGCC > NC_000913/2767713‑2767984 | taaaaTTTTGAAGTTCTGCAGCCACTTCAGAACTCCCTTTAGGTTCTACAGCTATGGGTATCTGAACGTATATTGCAGCTGATAAATCAGTTAACCCACTACGAGCCAGTGAACGGCATCATGGGTAAAACTGGGGCggg < 2:926804/139‑1 (MQ=255)aaaaaTTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTCCCGCGATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCggg < 1:1004085/140‑1 (MQ=255)aaaaaTTATGAAGTTCTGCACCCACTACAGAACTCCCTTTCCGGTATACCGCTAGGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCgtg < 2:926834/140‑3 (MQ=39) gCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCa < 1:1068576/140‑1 (MQ=255) aCTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGccc > 2:763496/1‑140 (MQ=255) aGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGttt > 2:757824/1‑140 (MQ=255) aaCTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTAGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGc > 1:374765/1‑140 (MQ=255) aaCTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTAGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGc > 1:374495/1‑140 (MQ=255) aCTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGcc > 1:736829/1‑140 (MQ=255) ccTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTg > 1:283750/1‑140 (MQ=255) ccTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTg > 2:431495/1‑140 (MQ=255) ccGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGt < 1:1155227/140‑1 (MQ=255) cTATGGGTATCTGAACGTCTCTTGCAGCAGATAAATCAGTTACCCCACTCCGTGCCTGTGATCGGCTTCATGGGTAAAACTGGGGCGGGAATGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTTAAGTATCGCCGGTCAg < 2:253938/140‑1 (MQ=37) cTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAg < 2:831654/140‑1 (MQ=255) tGGGTATCTGAACGTATATTGCAGCCGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGa > 2:1130383/1‑140 (MQ=255) ggTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATg > 2:737276/1‑140 (MQ=255) ggTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATg > 2:319269/1‑140 (MQ=255) ggTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATg > 2:319271/1‑140 (MQ=255) tATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTa < 1:178396/140‑1 (MQ=255) tATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTa < 1:852619/140‑1 (MQ=255) tATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTa < 2:996823/140‑1 (MQ=255) aTCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGAGGTAg > 2:524236/1‑140 (MQ=255) cTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCg < 2:654498/140‑1 (MQ=255) gAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGc < 2:259686/140‑1 (MQ=255) gAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGc < 2:709047/140‑1 (MQ=255) gAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGc < 1:229217/140‑1 (MQ=255) gAACGTATATTGCAGCAGAGAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGc < 1:990164/140‑1 (MQ=255) gATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCtt > 1:475323/1‑140 (MQ=255) gATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCtt > 1:475271/1‑140 (MQ=255) gATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCtt > 1:475239/1‑140 (MQ=255) gATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCtt > 1:475234/1‑140 (MQ=255) cACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCa < 1:1019150/140‑1 (MQ=255) cACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCa < 1:851615/140‑1 (MQ=255) cACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCa < 1:278118/140‑1 (MQ=255) cTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGa < 1:781864/140‑1 (MQ=255) tACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGAt > 2:94826/1‑140 (MQ=255) tACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGAt > 2:214930/1‑140 (MQ=255) tACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGAt > 2:214953/1‑140 (MQ=255) aGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGga > 1:1025782/1‑140 (MQ=255) aGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGga > 1:762592/1‑140 (MQ=255) gCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGgag > 1:1184166/1‑140 (MQ=255) cAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTGTCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGaa < 1:731043/140‑1 (MQ=255) cAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGaa < 1:775279/140‑1 (MQ=255) cAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGaa < 1:171705/140‑1 (MQ=255) cAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGaa < 1:171719/140‑1 (MQ=255) cAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGaa < 1:427110/140‑1 (MQ=255) cAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGaa < 1:775100/140‑1 (MQ=255) aGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAac < 1:214953/140‑1 (MQ=255) aGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAac < 1:214930/140‑1 (MQ=255) tGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAacac > 2:522047/1‑140 (MQ=255) tGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAacac > 1:971378/1‑140 (MQ=255) ggCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAACACTTTAt > 2:146877/1‑140 (MQ=255) ggCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAACACTTTAt > 2:146801/1‑140 (MQ=255) catcatGGGTAAAACTAGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAACACTTTATGa < 2:374765/140‑1 (MQ=255) catcatGGGTAAAACTAGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAACACTTTATGa < 2:374495/140‑1 (MQ=255) ggggCGGGTAAGAGTAGGCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGAAGTTTGGAGAACACTTTATGACCATCGTGGATCTGcc < 1:689133/140‑1 (MQ=255) ggggCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAACACTTTATGACCATCGTGGATCTGcc < 1:698680/140‑1 (MQ=255) | AAAAATTTTGAAGTTCTGCAGCCACTACAGAACTCCCTTTCCGGTCTACCGCTATGGGTATCTGAACGTATATTGCAGCAGATAAATCAGTTAACCCACTACGAGCCAGTGATCGGCATCATGGGTAAAACTGGGGCGGGAAAGAGTAGCCTTTGCAATGCCCTGTTTGCCGGTGAAGTATCGCCGGTCAGCGATGTAGCGGCCTGTACACGTGATCCTTTACGCTTTCGCCTGCAGATTGGAGAACACTTTATGACCATCGTGGATCTGCC > NC_000913/2767713‑2767984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |