Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,830,202	G→T	6.0%	L130L (CTG→CTT)	srlQ →	D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,830,202	0	G	T	6.0%	240.3 / 2.8	67	L130L (CTG→CTT)	srlQ	D‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (18/45);  new base T (2/2);  total (20/47)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

ATGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGAT  >  NC_000913/2830068‑2830341                                                                                                                                       |                                                                                                                                            atgatCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTTGGCCt                                                                                                                                        >  1:1113846/1‑140 (MQ=255) atgatCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTTGGCCt                                                                                                                                        >  1:1113878/1‑140 (MQ=255)  tgatCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTg                                                                                                                                       <  2:253738/140‑1 (MQ=255)  tgatCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTg                                                                                                                                       <  2:253774/140‑1 (MQ=255)  tgatCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTAGTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTg                                                                                                                                       <  2:633657/140‑1 (MQ=255)  tgatCGAAAGCCGCGATGTGATGCTGTTTATCTCGTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTg                                                                                                                                       <  1:391080/140‑1 (MQ=255)    atCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTggc                                                                                                                                     <  1:74576/140‑1 (MQ=255)            ccGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAg                                                                                                                             <  1:864872/140‑1 (MQ=255)            ccGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAg                                                                                                                             <  1:1163028/140‑1 (MQ=255)            ccGCGATGTGATGCTGTTGATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAg                                                                                                                             <  2:455887/140‑1 (MQ=255)               cgATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCgg                                                                                                                          >  1:358023/1‑140 (MQ=255)                 aTGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTg                                                                                                                        <  2:398087/140‑1 (MQ=255)                      aTGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGa                                                                                                                   <  1:46418/140‑1 (MQ=255)                         cTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTGCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGatat                                                                                                                <  1:665600/140‑1 (MQ=255)                         cTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGatat                                                                                                                <  2:777813/140‑1 (MQ=255)                         cTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGatat                                                                                                                <  1:59352/140‑1 (MQ=255)                         cTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGatat                                                                                                                <  1:631297/140‑1 (MQ=255)                                 ctctTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAg                                                                                                        <  1:413424/140‑1 (MQ=255)                                      aCTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAAcgc                                                                                                   >  2:361284/1‑140 (MQ=255)                                       cTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAAcgcg                                                                                                  <  1:372024/140‑1 (MQ=255)                                       cTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAAcgcg                                                                                                  <  1:790527/140‑1 (MQ=255)                                            gTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATTACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGcc                                                                                             >  2:64438/1‑140 (MQ=255)                                             tGGCGCGAAGGAACTGTATCTTATTATTACGCGACTGGAAGATAAATCTATCGCCCTGCTGCCGATGACCGGCAAACCGACGTCACCGCTGGGCCTCGCGGCAAAAGCGGTGCTGGAAATCTCCTTAGAACGCGAAGCCt                                                                                            <  2:815501/140‑1 (MQ=255)                                                    aaGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGGGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGAt                                                                                     <  2:906945/140‑1 (MQ=255)                                                    aaGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGAt                                                                                     <  2:479615/140‑1 (MQ=255)                                                     aGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATg                                                                                    <  1:372366/140‑1 (MQ=255)                                                                 tGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCg                                                                        <  1:480494/140‑1 (MQ=255)                                                                  gATTATTCCGCTTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCTCTGGGCCAGGCGGCATATTCGGAGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGGACCTTGCGCCGa                                                                       >  2:575215/1‑140 (MQ=255)                                                                  gATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGa                                                                       >  2:227259/1‑140 (MQ=255)                                                                   attattCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGAc                                                                      >  2:161193/1‑140 (MQ=255)                                                                     tattCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAg                                                                                                                             >  1:785520/1‑83 (MQ=255)                                                                     tattCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAg                                                                                                                             <  2:785520/83‑1 (MQ=255)                                                                       ttCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTcc                                                                  >  2:1017591/1‑140 (MQ=255)                                                                       ttCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTcc                                                                  >  2:1017641/1‑140 (MQ=255)                                                                        tCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCa                                                                 <  2:858858/140‑1 (MQ=255)                                                                        tCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCAGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCa                                                                 >  1:103190/1‑140 (MQ=255)                                                                           gcgTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCa                                                              <  2:779066/140‑1 (MQ=255)                                                                           gcgTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCa                                                              <  2:778769/140‑1 (MQ=255)                                                                           gcgTCTGGAAGATAAATCTATCGCGCTGCTGGCGACGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAg                                                                                               <  2:1197372/107‑1 (MQ=255)                                                                           gcgTCTGGAAGATAAATCTATCGCGCTGCTGGCGACGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAg                                                                                               >  1:1197372/1‑107 (MQ=255)                                                                            cgTCTGGATGATAATTCTATCGCGCTGCTGGCGATGACCTGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCAc                                                             <  1:886675/140‑1 (MQ=255)                                                                            cgTCTGGAAGATATATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCAc                                                             <  1:146097/140‑1 (MQ=255)                                                                            cgTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCAc                                                             <  1:21137/140‑1 (MQ=255)                                                                            cgTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCAc                                                             <  2:184114/140‑1 (MQ=255)                                                                            cgTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCAc                                                             <  2:371161/140‑1 (MQ=255)                                                                             gTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCTAACCGACGTCACCGCTGGGCCTGGCGGCAAATGCGGTGCTGGATATCTCCGTAGTACGCGAAGCCTGCCCGTTGCACCTTGCGCCGACCTCCAGCAcc                                                            >  2:1076937/1‑140 (MQ=255)                                                                             gTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCAcc                                                            <  1:841147/140‑1 (MQ=255)                                                                                  gAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCaa                                                       <  2:105298/140‑1 (MQ=255)                                                                                  gAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCaa                                                       <  2:105346/140‑1 (MQ=255)                                                                                      aTAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATAcc                                                   <  2:256741/140‑1 (MQ=255)                                                                                      aTAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATAcc                                                   <  2:256922/140‑1 (MQ=255)                                                                                      aTAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATAcc                                                   <  2:15509/140‑1 (MQ=255)                                                                                      aTAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATAcc                                                   <  2:15647/140‑1 (MQ=255)                                                                                      aTAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATAcc                                                   <  2:15525/140‑1 (MQ=255)                                                                                           tCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCtgat                                              <  2:512087/140‑1 (MQ=255)                                                                                               tCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGatgatg                                          >  1:282707/1‑140 (MQ=255)                                                                                               acgcgCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGTCGGCAAAAGCGGTGCTGGATATCTCCGTCGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGatgatg                                          >  1:282601/2‑140 (MQ=255)                                                                                                 gcgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCTCCGTCAATACACTGATGATtgg                                        >  2:1227038/1‑140 (MQ=255)                                                                                                 gcgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATggg                                        >  2:1227032/1‑140 (MQ=255)                                                                                                  cgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGt                                       >  1:647309/1‑140 (MQ=255)                                                                                                  cgcTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTGGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGt                                       >  1:647327/1‑140 (MQ=255)                                                                                                   gctgctGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTg                                      <  2:700094/140‑1 (MQ=255)                                                                                                   gctgctGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGAACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTg                                      <  2:580214/140‑1 (MQ=255)                                                                                                       ctgGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGAcgc                                  <  2:1188085/140‑1 (MQ=255)                                                                                                        tgGCGATGACCGGCAAACCGACGTCACCGCTTGGCCTGGCGTCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTTATGATGGGTGAcgcg                                 <  2:1113846/140‑1 (MQ=255)                                                                                                        tgGCGATGACCGGCAAACCGACGTCACCGCTTGGCCTGGCGTCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTTATGATGGGTGAcgcg                                 <  2:1113878/140‑1 (MQ=255)                                                                                                                     cAAACCGACGTCACCGCTGGGCCTGGCGGCTAAAGCGGTGCTGGATTTCTCCGTAGATCGCGACGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCgg                    <  2:115200/140‑1 (MQ=255)                                                                                                                                       gggCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGAAGGGTGACGCGCTGGCGATGGCGGTCAAGCAGGCGCGCGGAt  >  2:1134534/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                            ATGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAAAAGCGGTGCTGGATATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGAT  >  NC_000913/2830068‑2830341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑