Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,832,097	C→A	5.7%	G65C (GGT→TGT)	norR ←	anaerobic nitric oxide reductase DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,832,097	0	C	A	5.7%	247.7 / 2.6	70	G65C (GGT→TGT)	norR	anaerobic nitric oxide reductase DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (34/32);  new base A (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCA  >  NC_000913/2831958‑2832233                                                                                                                                             |                                                                                                                                         tGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTAcc                                                                                                                                          >  1:702350/1‑140 (MQ=255)   aaCCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGa                                                                                                                                        >  2:598523/1‑140 (MQ=255)      cTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTa                                                                                                                                     <  2:422712/140‑1 (MQ=255)      cTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTa                                                                                                                                     <  2:531186/140‑1 (MQ=255)      cTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAAGTCGCTGTCTGCGGGAAAGCGCACCACCTCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTa                                                                                                                                     <  2:879488/140‑1 (MQ=255)        tCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACAACCCCGGCGCGGGCATTCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCAACCGAGTACa                                                                                                                                   >  2:719089/1‑140 (MQ=255)          aGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATc                                                                                                                                 <  1:622695/140‑1 (MQ=255)          aGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATc                                                                                                                                 >  1:1139749/1‑140 (MQ=255)          aGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATc                                                                                                                                 >  1:1139772/1‑140 (MQ=255)          aGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATc                                                                                                                                 <  1:431572/140‑1 (MQ=255)                tcCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGTGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGc                                                                                                                           >  1:645455/1‑140 (MQ=255)                tcCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGc                                                                                                                           >  2:244168/1‑140 (MQ=255)                tcCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGc                                                                                                                           >  2:265175/1‑140 (MQ=255)                tcCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGc                                                                                                                           >  2:90380/1‑140 (MQ=255)                tcCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACAGCCTTTGc                                                                                                                           >  2:813144/1‑140 (MQ=255)                          gAATCAAACCGTCATAGGGATCGGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAAACGATTCCAGCCGTGTATGCCCTGCCACCGCAAACCGACAACAGAGTCAAACCTTTGCCAGACCgac                                                                                                                  >  1:1166867/1‑138 (MQ=255)                          gAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACAGAGTACATCCTTTGCCAGACCGTCg                                                                                                                 >  1:280190/1‑140 (MQ=255)                                  ccGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAg                                                                                                         <  2:393656/140‑1 (MQ=255)                                  ccGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAg                                                                                                         <  2:1187466/140‑1 (MQ=255)                                  ccGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAg                                                                                                         <  2:393615/140‑1 (MQ=255)                                             aTC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCTCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTGTGCCAGACCGACCATGGCAAGCGCAATAAACt                                                                                              >  2:653819/1‑140 (MQ=255)                                             aTC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGACTAAACt                                                                                              >  2:809951/1‑140 (MQ=255)                                             aTC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACt                                                                                              >  1:925836/1‑140 (MQ=255)                                             aTC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACt                                                                                              >  2:653736/1‑140 (MQ=255)                                               c‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                                                            >  2:1097762/1‑140 (MQ=255)                                               c‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                                                            >  1:1110236/1‑140 (MQ=255)                                               c‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                                                            >  2:756073/1‑140 (MQ=255)                                                  ggCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCcg                                                                                          >  1:724255/1‑140 (MQ=255)                                                  ggCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCcg                                                                                          <  1:105039/140‑1 (MQ=255)                                                    cAATTCGCTGTCTGCGGGAAAGCGCTCCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCcgcg                                                                                        <  2:1152583/140‑1 (MQ=255)                                                       ttCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAAt                                                                                     >  1:1007913/1‑140 (MQ=255)                                                       ttCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAAt                                                                                     >  1:192741/1‑140 (MQ=255)                                                                 gCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAg                                                                           <  2:38358/140‑1 (MQ=255)                                                                        aGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCa                                                                    >  2:7076/1‑140 (MQ=255)                                                                        aGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCa                                                                    >  1:927162/1‑140 (MQ=255)                                                                        aGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCa                                                                    >  1:861039/1‑140 (MQ=255)                                                                        aGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCa                                                                    >  1:629467/1‑140 (MQ=255)                                                                            caccacATCCCCGTCGCGGGCAATCGCTTCCAGCCGTTGATGCCCTTCCAGCACAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACg                                                                <  2:982261/140‑1 (MQ=255)                                                                            caccacATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACg                                                                >  2:1201335/1‑140 (MQ=255)                                                                               cacaTCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCag                                                             >  2:927515/1‑139 (MQ=255)                                                                               cacaTCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCAt                                                             >  2:224179/1‑140 (MQ=255)                                                                               cacaTCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGGCCATCCTTTGCCAGTCCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCag                                                             >  2:927602/1‑139 (MQ=255)                                                                               cacaTCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGAAGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCCATGCCAAGCGGTCTAAACAGCCGCGAATCGTAACGTAGCAACGCAGACGCAt                                                             >  2:927589/1‑140 (MQ=255)                                                                                 caTCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCg                                                           <  1:539437/140‑1 (MQ=255)                                                                                    ccccGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTACCAGCGCAAAGCGTCTACCGAGTACAACCTTTGCCAGACCGTCGATTGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACCCAGACGCAACGCAc                                                        >  1:1145660/1‑140 (MQ=255)                                                                                         gcgcGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAg                                                   >  1:966255/1‑140 (MQ=255)                                                                                         gcgcGGGCAATCGATTCCAGCCGTGGATGACATTCCAGCGCAAAGCGTCTAACGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTTCCGCGAATCGTAAAGTAGCAACGCAGACGCATCTCACTCCAg                                                   >  1:45623/1‑140 (MQ=255)                                                                                            cgGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCAc                                                >  1:197596/1‑140 (MQ=255)                                                                                                  aTCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACg                                          <  2:73160/140‑1 (MQ=255)                                                                                                    cGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTa                                        <  1:482905/140‑1 (MQ=255)                                                                                                       ttCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCg                                     <  1:128103/140‑1 (MQ=255)                                                                                                       ttCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCg                                     <  2:702350/140‑1 (MQ=255)                                                                                                        tCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgt                                    <  1:454396/140‑1 (MQ=255)                                                                                                         ccAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                   <  2:252090/140‑1 (MQ=255)                                                                                                         ccAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                   <  2:733270/140‑1 (MQ=255)                                                                                                         ccAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACAGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                   <  2:280190/140‑1 (MQ=255)                                                                                                         ccAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACAGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                   <  2:1166867/140‑1 (MQ=255)                                                                                                                 ggATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAg                           <  2:568559/140‑1 (MQ=255)                                                                                                                  gATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAgg                          <  2:231055/140‑1 (MQ=255)                                                                                                                  gATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAgg                          <  2:713307/140‑1 (MQ=255)                                                                                                                           ccAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaaaa                 <  1:159007/140‑1 (MQ=255)                                                                                                                           ccAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaaaa                 <  2:374147/140‑1 (MQ=255)                                                                                                                            cAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAAc                >  1:4767/1‑140 (MQ=255)                                                                                                                             aGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACg               <  1:216507/140‑1 (MQ=255)                                                                                                                             aGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACg               <  1:689918/140‑1 (MQ=255)                                                                                                                              gcgcATAGCGTCTTCCGAGTACATCATTGGCCAGAGCGTCGATGGCAAGGGGAATATACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGTCGTATCGTGGTGATCAGGCGCTGATAACGa              <  2:683030/140‑1 (MQ=255)                                                                                                                              gagcAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGAAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGa              <  2:402868/138‑1 (MQ=255)                                                                                                                                   aaGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTg         <  1:1123911/140‑1 (MQ=255)                                                                                                                                   aaGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTg         <  1:1123883/140‑1 (MQ=255)                                                                                                                                        tCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGAc    <  1:265175/140‑1 (MQ=255)                                                                                                                                          tACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCa  >  1:520438/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                         TGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATC‑GGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCA  >  NC_000913/2831958‑2832233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑