Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,843,836 G→T 5.1% L125L (CTC→CTA hycG ← hydrogenase 3 and formate hydrogenase complex, HycG subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,843,8360GT 5.1% 296.8 / 2.4 79L125L (CTC→CTAhycGhydrogenase 3 and formate hydrogenase complex, HycG subunit
Reads supporting (aligned to +/- strand):  ref base G (34/41);  new base T (2/2);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GTGAATTTTCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGT  >  NC_000913/2843707‑2843965
                                                                                                                                 |                                                                                                                                 
gTGAATTTTCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGa                                                                                                                         >  1:1071917/1‑140 (MQ=255)
gTGAATTTTCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGACCCACACGCAGTAGAGATCGTGGa                                                                                                                         >  1:1072070/1‑140 (MQ=255)
 tGAATTTTCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGaa                                                                                                                        >  2:1042204/1‑140 (MQ=255)
 tGAATTTTCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGaa                                                                                                                        >  2:790596/1‑140 (MQ=255)
   aaTTTTCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGa                                                                                                                      <  2:545750/140‑1 (MQ=255)
         cTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGc                                                                                                                <  1:1016737/140‑1 (MQ=255)
         cTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGc                                                                                                                <  1:1038126/140‑1 (MQ=255)
         cTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGc                                                                                                                <  1:1016408/140‑1 (MQ=255)
          tGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGcc                                                                                                               <  1:678280/140‑1 (MQ=255)
                 gcagGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTATAGATCGTGGAAGATCCCGCCACTGTTa                                                                                                        >  2:110420/1‑140 (MQ=255)
                 gcagGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTATAGATCGTGGAAGATCCCGCCACTGTTa                                                                                                        >  2:110602/1‑140 (MQ=255)
                 gcagGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTa                                                                                                        >  2:770192/1‑140 (MQ=255)
                 gcagGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTa                                                                                                        >  2:281489/1‑140 (MQ=255)
                        gAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTATAGATCGTGGAAGATCCCGCCACTGTTACCGCAgg                                                                                                 <  1:110602/140‑1 (MQ=255)
                        gAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTATAGATCGTGGAAGATCCCGCCACTGTTACCGCAgg                                                                                                 <  1:110420/140‑1 (MQ=255)
                        gAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAgg                                                                                                 <  1:281489/140‑1 (MQ=255)
                         aGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGc                                                                                                >  1:293236/1‑140 (MQ=255)
                                           tACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAAt                                                                              >  2:578825/1‑140 (MQ=255)
                                                cGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCg                                                                         >  1:321754/1‑140 (MQ=255)
                                                cGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCg                                                                         >  1:321832/1‑140 (MQ=255)
                                                  tGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAATTTTTCggg                                                                       >  1:996545/1‑140 (MQ=255)
                                                  tGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCggg                                                                       >  1:996990/1‑140 (MQ=255)
                                                  tGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCggg                                                                       >  1:801564/1‑140 (MQ=255)
                                                  tGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCggg                                                                       >  1:377741/1‑140 (MQ=255)
                                                  tGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCggg                                                                       >  1:378050/1‑140 (MQ=255)
                                                  tGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCggg                                                                       >  1:757870/1‑140 (MQ=255)
                                                   ggcggcAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGt                                                                      <  1:362904/140‑1 (MQ=255)
                                                   ggcggcAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGt                                                                      <  1:751484/140‑1 (MQ=255)
                                                        cAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGgc                                                                 <  2:547958/140‑1 (MQ=255)
                                                        cAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGgc                                                                 <  2:58750/140‑1 (MQ=255)
                                                               tGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACt                                                          <  2:246800/140‑1 (MQ=255)
                                                               tGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACt                                                          <  2:246799/140‑1 (MQ=255)
                                                               tGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACt                                                          <  2:1158652/140‑1 (MQ=255)
                                                                     gCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCcacg                                                    >  1:833934/1‑140 (MQ=255)
                                                                      cAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCcacgc                                                   <  1:374423/140‑1 (MQ=255)
                                                                         ccAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCcacgcacg                                                <  2:512845/140‑1 (MQ=255)
                                                                         ccAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCcacgcacg                                                <  1:251021/140‑1 (MQ=255)
                                                                         ccAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCcacgcacg                                                <  1:250941/140‑1 (MQ=255)
                                                                         ccAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCcacgcacg                                                <  1:385728/140‑1 (MQ=255)
                                                                           aGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCAcgcacgca                                              <  1:1030276/140‑1 (MQ=255)
                                                                              gATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCAcgcagcg                                           <  2:447281/140‑1 (MQ=255)
                                                                                tataAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCAcgcagcgca                                         <  1:914024/140‑1 (MQ=255)
                                                                                tataAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCAcgcagcgca                                         <  1:266363/140‑1 (MQ=255)
                                                                                  taAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAgg                                       >  2:78171/1‑140 (MQ=255)
                                                                                   aaaCATCCACAGGGACAATTTTCTCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAggg                                      <  2:924353/140‑1 (MQ=255)
                                                                                   aaaCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGATACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCCCGCAGCGCAggg                                      >  2:649971/1‑140 (MQ=255)
                                                                                   aaaCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACAGTTACCGCAGGCACCGGAGGCGATACAAATTTTCGGGTCCGGCGCGGCCTGCCACGCACGCACCGCAggg                                      >  2:698098/1‑140 (MQ=255)
                                                                                     aCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGa                                    <  1:942094/140‑1 (MQ=255)
                                                                                     aCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGa                                    <  2:1008637/140‑1 (MQ=255)
                                                                                     aCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGa                                    <  1:942030/140‑1 (MQ=255)
                                                                                     aCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGa                                    <  1:822773/140‑1 (MQ=255)
                                                                                     aCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGa                                    <  2:1180974/140‑1 (MQ=255)
                                                                                     aCATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGa                                    <  1:456519/140‑1 (MQ=255)
                                                                                        tCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCg                                 <  1:1189699/140‑1 (MQ=255)
                                                                                        tCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCg                                 <  2:559605/140‑1 (MQ=255)
                                                                                         ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGc                                >  2:474517/1‑140 (MQ=255)
                                                                                         ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGc                                >  1:162212/1‑140 (MQ=255)
                                                                                         ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGc                                >  2:731108/1‑140 (MQ=255)
                                                                                         ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGc                                >  2:669556/1‑140 (MQ=255)
                                                                                         ccACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGc                                >  1:394699/1‑140 (MQ=255)
                                                                                           acaGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCAt                              >  1:424466/1‑140 (MQ=255)
                                                                                           acaGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCAt                              >  1:424101/1‑140 (MQ=255)
                                                                                           acaGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCAt                              >  1:357575/1‑140 (MQ=255)
                                                                                            caGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCAtt                             <  1:828087/140‑1 (MQ=255)
                                                                                            caGGGACAATTCTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCAtt                             <  1:828561/140‑1 (MQ=255)
                                                                                                    aTTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGt                     >  2:762069/1‑140 (MQ=255)
                                                                                                           ccGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACcgcg              <  1:1042204/140‑1 (MQ=255)
                                                                                                              tACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCg           <  2:162212/140‑1 (MQ=255)
                                                                                                               aCCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCCCCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCgg          >  2:157039/1‑140 (MQ=255)
                                                                                                               aCCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCgg          >  1:1143517/1‑140 (MQ=255)
                                                                                                               aCCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCAGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCgg          >  1:956184/1‑140 (MQ=255)
                                                                                                               aCCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGGAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCgg          >  2:157043/1‑140 (MQ=255)
                                                                                                                 cGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTa        <  1:179902/140‑1 (MQ=255)
                                                                                                                 cGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTa        <  1:78171/140‑1 (MQ=255)
                                                                                                                  gCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTaa       <  1:260141/140‑1 (MQ=255)
                                                                                                                    cccACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAAc     <  2:1117784/140‑1 (MQ=255)
                                                                                                                       acacGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGt  <  2:174582/140‑1 (MQ=255)
                                                                                                                              gTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAAc     <  2:553550/130‑1 (MQ=255)
                                                                                                                              gTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAAc     >  1:553550/1‑130 (MQ=255)
                                                                                                                                 |                                                                                                                                 
GTGAATTTTCTGCTCCAGCAGGCCGAGCGCCATTGCAAAGCCGTACAGCGTGGCGGCAGGCGTTGGCGGGCAGCCAGGGATATAAACATCCACAGGGACAATTTTATCCGTACCGCCCCACACGCAGTAGAGATCGTGGAAGATCCCGCCACTGTTACCGCAGGCACCGTAGGAGATACAAATTTTCGGGTCCGGCGCGGACTGCCACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGT  >  NC_000913/2843707‑2843965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: