Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,142,565 A→G 6.3% L142P (CTG→CCG)  hybC ← hydrogenase 2, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,142,5650AG 6.3% 206.8 / 4.6 63L142P (CTG→CCG) hybChydrogenase 2, large subunit
Reads supporting (aligned to +/- strand):  ref base A (30/29);  new base G (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

ATCAGGTTCACTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGC  >  NC_000913/3142433‑3142701
                                                                                                                                    |                                                                                                                                        
aTCAGGTTCACTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCa                                                                                                                                   >  2:1100808/1‑140 (MQ=255)
aTCAGGTTCACTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCa                                                                                                                                   >  2:1100902/1‑140 (MQ=255)
     gTTCACTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCg                                                                                                                              >  2:1138936/1‑140 (MQ=255)
     gTTCACTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCg                                                                                                                              >  2:300352/1‑140 (MQ=255)
             ccGGCGGCACATACATCCCCGGAAGACCCCAGTACCCATTACCCAAAAAACCCAACTCACCGCTGGCAACCAGATCTTTCATCTTGTTCTGAACTTTGCAGAACACTACCCCACTGTTCAGGTGCCAGCTCGAAAcccca                                                                                                                      <  2:334405/140‑2 (MQ=255)
               ggcggcAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCGGGTGCCAGGTCGAAACGCCttt                                                                                                                    >  2:27519/1‑140 (MQ=255)
               ggcggcAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCGGGTGCCAGGTCGAAACGCCttt                                                                                                                    >  2:27514/1‑140 (MQ=255)
                         tCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCg                                                                                                          <  1:710983/140‑1 (MQ=255)
                                   aTGACCCCAGTAGCCCTTAGCGAAAATCCCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTttgg                                                                                                <  1:729562/140‑1 (MQ=255)
                                        cccAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTggg                                                                                           <  1:1084315/140‑1 (MQ=255)
                                           aggaGCCATTCGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCa                                                                                        <  2:695539/137‑1 (MQ=255)
                                           aGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTAGATCTTGTTCTGAACTTTGGAGAACACTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCa                                                                                        <  2:694801/140‑1 (MQ=255)
                                            gTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAg                                                                                       >  1:533853/1‑140 (MQ=255)
                                            gTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAg                                                                                       >  1:972003/1‑140 (MQ=255)
                                            gTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAg                                                                                       >  1:1058735/1‑140 (MQ=255)
                                            gTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAg                                                                                       >  1:1114076/1‑140 (MQ=255)
                                              aGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCt                                                                                     <  2:384738/140‑1 (MQ=255)
                                                ccATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTtg                                                                                   <  1:180326/140‑1 (MQ=255)
                                                ccATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCCGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTtg                                                                                   <  1:1057909/140‑1 (MQ=255)
                                                ccATTAGCGAAAATACCAAACTGACCGCTGGCAACCAGAACTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTACGGAGGCTTTGGTTGGGTCAGCTtg                                                                                   <  1:746056/140‑1 (MQ=255)
                                                 cATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTtgc                                                                                  >  2:1108339/1‑140 (MQ=255)
                                                  aTTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTtgca                                                                                 >  2:107032/1‑140 (MQ=255)
                                                         aaaaTACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGa                                                                          <  2:1124473/140‑1 (MQ=255)
                                                            aTACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGt                                                                       <  1:1118476/140‑1 (MQ=255)
                                                            aTACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGt                                                                       <  1:1050533/140‑1 (MQ=255)
                                                            aTACCAAACTGACCGCTGCCAACCAGATCTTTGATCTTGTTCTGAACTATGGGGAACTCTTCCGGACTGTTCAGGTGCCAGGACGAAAAGCCTTTCAGCATTACGTAGGCTTTGGTTGGGTCAGCTTGCTGTGCAGAAGt                                                                       <  2:182496/140‑1 (MQ=255)
                                                                ccAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGAtt                                                                   >  2:1218190/1‑139 (MQ=255)
                                                                         cGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAg                                                          >  1:1165320/1‑140 (MQ=255)
                                                                                      aTCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGaaa                                             <  2:331949/140‑1 (MQ=255)
                                                                                      aTCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGaaa                                             <  2:332371/140‑1 (MQ=255)
                                                                                      aTCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGaaa                                             <  2:453349/140‑1 (MQ=255)
                                                                                       acTTTGCTCATGGTCTGAACTTTGGTGAACTCTTCCGGACAGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGCGTCAGCTTGCAGTGCAGAAGAGATGTCCACCCAGTCCAGCGCCGAAAg                                            <  2:267243/139‑1 (MQ=255)
                                                                                          ttGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTg                                         >  1:536916/1‑140 (MQ=255)
                                                                                           tGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCGGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGa                                        <  1:27519/140‑1 (MQ=255)
                                                                                           tGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCGGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGa                                        <  1:27514/140‑1 (MQ=255)
                                                                                                ttGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCTGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGaa                                   <  2:497925/140‑1 (MQ=255)
                                                                                                ttGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGaa                                   <  2:254362/140‑1 (MQ=255)
                                                                                                ttGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGaa                                   <  1:342572/140‑1 (MQ=255)
                                                                                                ttGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGaa                                   >  2:459997/1‑140 (MQ=255)
                                                                                                  gTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAAt                                 >  1:792934/1‑140 (MQ=255)
                                                                                                  gTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAAt                                 >  1:884018/1‑140 (MQ=255)
                                                                                                  gTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAAt                                 >  1:1034385/1‑140 (MQ=255)
                                                                                                  gTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGGTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAAt                                 >  1:1034497/1‑140 (MQ=255)
                                                                                                   ttCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATg                                <  1:921305/140‑1 (MQ=255)
                                                                                                     cTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGaa                              <  1:496372/140‑1 (MQ=255)
                                                                                                          cTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAata                         <  1:324584/140‑1 (MQ=255)
                                                                                                             tGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATgg                      >  1:939832/1‑140 (MQ=255)
                                                                                                              ggTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt                     <  1:527687/140‑1 (MQ=255)
                                                                                                              ggTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt                     <  1:38795/140‑1 (MQ=255)
                                                                                                                  aaCTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATg                 >  1:298114/1‑140 (MQ=255)
                                                                                                                  aaCTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATg                 >  1:426641/1‑140 (MQ=255)
                                                                                                                  aaCTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATg                 >  1:422558/1‑140 (MQ=255)
                                                                                                                             aCTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTgcg      <  1:864786/140‑1 (MQ=255)
                                                                                                                             aCTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTgcg      <  1:863991/140‑1 (MQ=255)
                                                                                                                             aCTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTgcg      <  1:317867/140‑1 (MQ=255)
                                                                                                                               tGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCa    >  1:960760/1‑140 (MQ=255)
                                                                                                                               tGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCa    >  1:412205/1‑140 (MQ=255)
                                                                                                                                 ttCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGc  >  2:628424/1‑140 (MQ=255)
                                                                                                                                 ttCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGc  >  2:1117605/1‑140 (MQ=255)
                                                                                                                                 ttCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGc  >  1:1058011/1‑140 (MQ=255)
                                                                                                                                 ttCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGc  >  2:723406/1‑140 (MQ=255)
                                                                                                                                 ttCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGc  >  2:755836/1‑140 (MQ=255)
                                                                                                                                 ttCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGc  >  2:959284/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                        
ATCAGGTTCACTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGC  >  NC_000913/3142433‑3142701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: